acidithiobacillus_ferrivorans_medium

CultureMech:000693 bacterial DEFINED LIQUID
Original name: ACIDITHIOBACILLUS FERRIVORANS MEDIUM

Overview

External ontology
mediadive.medium:1234 (source link unavailable) — ACIDITHIOBACILLUS FERRIVORANS MEDIUM
Applications
  • Microbial cultivation
Notes
Source: DSMZ | Link: https://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium1234.pdf

Composition

flowchart LR
    CultureMech_000693["acidithiobacillus_ferrivorans_medium"]
    ing0_Na2SO4["Na2SO4"]
    CultureMech_000693 --> ing0_Na2SO4
    CHEBI_32149["CHEBI:32149"]
    ing0_Na2SO4 -.-> CHEBI_32149
    ing1__NH4_2SO4["(NH4)2SO4"]
    CultureMech_000693 --> ing1__NH4_2SO4
    CHEBI_62946["CHEBI:62946"]
    ing1__NH4_2SO4 -.-> CHEBI_62946
    ing2_KCl["KCl"]
    CultureMech_000693 --> ing2_KCl
    CHEBI_32588["CHEBI:32588"]
    ing2_KCl -.-> CHEBI_32588
    ing3_MgSO4_x_7_H2O["MgSO4 x 7 H2O"]
    CultureMech_000693 --> ing3_MgSO4_x_7_H2O
    CHEBI_31795["CHEBI:31795"]
    ing3_MgSO4_x_7_H2O -.-> CHEBI_31795
    ing4_K2HPO4["K2HPO4"]
    CultureMech_000693 --> ing4_K2HPO4
    CHEBI_131527["CHEBI:131527"]
    ing4_K2HPO4 -.-> CHEBI_131527
    ing5_Ca_NO3_2_x_4_H2O["Ca(NO3)2 x 4 H2O"]
    CultureMech_000693 --> ing5_Ca_NO3_2_x_4_H2O
    CHEBI_86159["CHEBI:86159"]
    ing5_Ca_NO3_2_x_4_H2O -.-> CHEBI_86159
    ing6__NH4_2Ni_SO4_2_x_6_H2O["(NH4)2Ni(SO4)2 x 6 H2O"]
    CultureMech_000693 --> ing6__NH4_2Ni_SO4_2_x_6_H2O
    CHEBI_86149["CHEBI:86149"]
    ing6__NH4_2Ni_SO4_2_x_6_H2O -.-> CHEBI_86149
    ing7_K2S4O6["K2S4O6"]
    CultureMech_000693 --> ing7_K2S4O6
    CHEBI_86466["CHEBI:86466"]
    ing7_K2S4O6 -.-> CHEBI_86466
    ing8_FeSO4_x_7_H2O["FeSO4 x 7 H2O"]
    CultureMech_000693 --> ing8_FeSO4_x_7_H2O
    CHEBI_75836["CHEBI:75836"]
    ing8_FeSO4_x_7_H2O -.-> CHEBI_75836
    ing9_Nitrilotriacetic_acid["Nitrilotriacetic acid"]
    CultureMech_000693 --> ing9_Nitrilotriacetic_acid
    CHEBI_44557["CHEBI:44557"]
    ing9_Nitrilotriacetic_acid -.-> CHEBI_44557
    ing10_MnSO4_x_H2O["MnSO4 x H2O"]
    CultureMech_000693 --> ing10_MnSO4_x_H2O
    CHEBI_86364["CHEBI:86364"]
    ing10_MnSO4_x_H2O -.-> CHEBI_86364
    ing11_NaCl["NaCl"]
    CultureMech_000693 --> ing11_NaCl
    CHEBI_26710["CHEBI:26710"]
    ing11_NaCl -.-> CHEBI_26710
    ing12_CoSO4_x_7_H2O["CoSO4 x 7 H2O"]
    CultureMech_000693 --> ing12_CoSO4_x_7_H2O
    CHEBI_91244["CHEBI:91244"]
    ing12_CoSO4_x_7_H2O -.-> CHEBI_91244
    ing13_CaCl2_x_2_H2O["CaCl2 x 2 H2O"]
    CultureMech_000693 --> ing13_CaCl2_x_2_H2O
    CHEBI_86158["CHEBI:86158"]
    ing13_CaCl2_x_2_H2O -.-> CHEBI_86158
    ing14_ZnSO4_x_7_H2O["ZnSO4 x 7 H2O"]
    CultureMech_000693 --> ing14_ZnSO4_x_7_H2O
    CHEBI_32312["CHEBI:32312"]
    ing14_ZnSO4_x_7_H2O -.-> CHEBI_32312
    ing15_CuSO4_x_5_H2O["CuSO4 x 5 H2O"]
    CultureMech_000693 --> ing15_CuSO4_x_5_H2O
    CHEBI_31440["CHEBI:31440"]
    ing15_CuSO4_x_5_H2O -.-> CHEBI_31440
    ing16_AlK_SO4_2_x_12_H2O["AlK(SO4)2 x 12 H2O"]
    CultureMech_000693 --> ing16_AlK_SO4_2_x_12_H2O
    CHEBI_86465["CHEBI:86465"]
    ing16_AlK_SO4_2_x_12_H2O -.-> CHEBI_86465
    ing17_H3BO3["H3BO3"]
    CultureMech_000693 --> ing17_H3BO3
    CHEBI_33118["CHEBI:33118"]
    ing17_H3BO3 -.-> CHEBI_33118
    ing18_Na2MoO4_x_2_H2O["Na2MoO4 x 2 H2O"]
    CultureMech_000693 --> ing18_Na2MoO4_x_2_H2O
    CHEBI_75213["CHEBI:75213"]
    ing18_Na2MoO4_x_2_H2O -.-> CHEBI_75213
    ing19_NiCl2_x_6_H2O["NiCl2 x 6 H2O"]
    CultureMech_000693 --> ing19_NiCl2_x_6_H2O
    CHEBI_34887["CHEBI:34887"]
    ing19_NiCl2_x_6_H2O -.-> CHEBI_34887
    ing20_Na2SeO3_x_5_H2O["Na2SeO3 x 5 H2O"]
    CultureMech_000693 --> ing20_Na2SeO3_x_5_H2O
    CHEBI_131361["CHEBI:131361"]
    ing20_Na2SeO3_x_5_H2O -.-> CHEBI_131361
    ing21_Na2WO4_x_2_H2O["Na2WO4 x 2 H2O"]
    CultureMech_000693 --> ing21_Na2WO4_x_2_H2O
    CHEBI_63939["CHEBI:63939"]
    ing21_Na2WO4_x_2_H2O -.-> CHEBI_63939

Ingredients (22)

# Ingredient Concentration Ontology Notes
1 Na2SO4
2Na.O4S 142.043 g/mol
1.4 G_PER_L CHEBI:32149
sodium sulfate
2 (NH4)2SO4
2H4N.O4S 132.141 g/mol
3 G_PER_L CHEBI:62946
ammonium sulfate
3 KCl
Cl.K 74.551 g/mol
0.1 G_PER_L CHEBI:32588
potassium chloride
4 MgSO4 x 7 H2O
7H2O.Mg.O4S 246.473 g/mol
3.5 G_PER_L CHEBI:31795
magnesium sulfate heptahydrate
 [Merged 2 duplicates: 0.5, 3.0]
5 K2HPO4
HO4P.2K 174.174 g/mol
0.05 G_PER_L CHEBI:131527
dipotassium hydrogen phosphate
6 Ca(NO3)2 x 4 H2O
Ca.4H2O.2NO3 236.146 g/mol
0.02 G_PER_L CHEBI:86159
calcium nitrate tetrahydrate
7 (NH4)2Ni(SO4)2 x 6 H2O
6H2O.2H4N.Ni.2O4S 394.987 g/mol
0.02 G_PER_L CHEBI:86149
ammonium nickel sulfate hexahydrate
8 K2S4O6
2K.O6S4 302.458 g/mol
0.8 G_PER_L CHEBI:86466
potassium tetrathionate
9 FeSO4 x 7 H2O
Fe.7H2O.O4S 278.013 g/mol
0.13 G_PER_L CHEBI:75836
iron(2+) sulfate heptahydrate
 [Merged 2 duplicates: 0.03, 0.1]
10 Nitrilotriacetic acid
C6H9NO6 191.139 g/mol
1.5 G_PER_L CHEBI:44557
nitrilotriacetic acid
11 MnSO4 x H2O
H2O.Mn.O4S 169.016 g/mol
0.5 G_PER_L CHEBI:86364
manganese(II) sulfate monohydrate
12 NaCl
Cl.Na 58.443 g/mol
1 G_PER_L CHEBI:26710
sodium chloride
13 CoSO4 x 7 H2O
Co.7H2O.O4S 281.101 g/mol
0.18 G_PER_L CHEBI:91244
cobalt(2+) sulfate heptahydrate
14 CaCl2 x 2 H2O
Ca.2Cl.2H2O 147.014 g/mol
0.1 G_PER_L CHEBI:86158
calcium chloride dihydrate
15 ZnSO4 x 7 H2O
7H2O.O4S.Zn 287.558 g/mol
0.18 G_PER_L CHEBI:32312
zinc sulfate heptahydrate
16 CuSO4 x 5 H2O
Cu.5H2O.O4S 249.684 g/mol
0.01 G_PER_L CHEBI:31440
copper(II) sulfate pentahydrate
17 AlK(SO4)2 x 12 H2O
Al.12H2O.K.2O4S 474.386 g/mol
0.02 G_PER_L CHEBI:86465
potassium aluminium sulfate dodecahydrate
18 H3BO3
H3BO3 61.833 g/mol
0.01 G_PER_L CHEBI:33118
boric acid
19 Na2MoO4 x 2 H2O
2H2O.MoO4.2Na 241.946 g/mol
0.01 G_PER_L CHEBI:75213
sodium molybdate dihydrate
20 NiCl2 x 6 H2O
Cl2Ni 129.599 g/mol
0.03 G_PER_L CHEBI:34887
nickel dichloride
21 Na2SeO3 x 5 H2O
5H2O.2Na.O3Se 263.012 g/mol
0.0003 G_PER_L CHEBI:131361
disodium selenite pentahydrate
22 Na2WO4 x 2 H2O
2H2O.2Na.O4W 329.846 g/mol
0.0004 G_PER_L CHEBI:63939
sodium tungstate dihydrate

Preparation

  1. Dissolve ingredients (except tetrathionate and ferrous sulfate), adjust pH to 2.5 with H2SO4 and autoclave. After cooling, add tetrathionate from a 100 mM stock solution and FeSO4 from a 10 mM stock solution (prepared in water adjusted to pH 2.0 with H2SO4). Both solutions should be freshly prepared and sterilized by filtration.
  2. First dissolve nitrilotriacetic acid and adjust pH to 6.5 with KOH, then add minerals. Adjust final to pH 7.0 with KOH.

Target organisms

No target organisms are recorded.

Provenance

TimestampCuratorActionNotes
2026-01-26T17:53:13.799152Z mediadive-import Imported from MediaDive Source: DSMZ, ID: 1234
2026-01-28T19:48:19.598341Z chebi-enrichment Added CHEBI ontology terms to ingredients Enriched using MicrobeMediaParam and MediaDive chemical mappings
2026-03-13T03:09:01.712905Z data-quality-cleanup-v1.0 Data quality fixes Merged 2 duplicate ingredient(s)
2026-03-13T06:18:57.514150Z culturemech-id-assigner-v1.0 Assigned CultureMech ID Assigned stable identifier: CultureMech:000693
2026-03-17T06:30:07.000000Z mediaingredientmech-enrichment-v1.0 Added MediaIngredientMech links Linked ingredients to MediaIngredientMech identifiers
2026-03-17T19:45:08.806832+00:00 normalize_media_names_to_snake_case.py NAME_NORMALIZED Normalized name from "ACIDITHIOBACILLUS FERRIVORANS MEDIUM" to "acidithiobacillus_ferrivorans_medium" for consistent matching
2026-06-05T07:40:47.345671+00:00 mim-legacy-to-chebi-migration-v1.0 Refreshed legacy MediaIngredientMech links to CHEBI keying Replaced 22 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme.
2026-06-10T07:46:52.612249+00:00 primary-term-label-canonicalization-v1.0 Canonicalized primary term label to OBO canonical (OAK) 4 primary term(s); ingredient name resolved by OAK sqlite:obo:chebi.