Overview
- External ontology
- mediadive.medium:1239 (source link unavailable) — DESULFURISPIRILLUM MEDIUM
- Applications
-
- Microbial cultivation
- Notes
- Source: DSMZ | Link: https://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium1239.pdf
Composition
flowchart LR
CultureMech_000698["desulfurispirillum_medium"]
ing0_NaHCO3["NaHCO3"]
CultureMech_000698 --> ing0_NaHCO3
CHEBI_32139["CHEBI:32139"]
ing0_NaHCO3 -.-> CHEBI_32139
ing1_NaCl["NaCl"]
CultureMech_000698 --> ing1_NaCl
CHEBI_26710["CHEBI:26710"]
ing1_NaCl -.-> CHEBI_26710
ing2_K2HPO4["K2HPO4"]
CultureMech_000698 --> ing2_K2HPO4
CHEBI_131527["CHEBI:131527"]
ing2_K2HPO4 -.-> CHEBI_131527
ing3_Na2CO3["Na2CO3"]
CultureMech_000698 --> ing3_Na2CO3
CHEBI_29377["CHEBI:29377"]
ing3_Na2CO3 -.-> CHEBI_29377
ing4_MgSO4["MgSO4"]
CultureMech_000698 --> ing4_MgSO4
CHEBI_32599["CHEBI:32599"]
ing4_MgSO4 -.-> CHEBI_32599
ing5_Yeast_extract["Yeast extract"]
CultureMech_000698 --> ing5_Yeast_extract
ing6_Sodium_acetate["Sodium acetate"]
CultureMech_000698 --> ing6_Sodium_acetate
CHEBI_32954["CHEBI:32954"]
ing6_Sodium_acetate -.-> CHEBI_32954
ing7_KNO3["KNO3"]
CultureMech_000698 --> ing7_KNO3
CHEBI_63043["CHEBI:63043"]
ing7_KNO3 -.-> CHEBI_63043
ing8_Na2S["Na2S"]
CultureMech_000698 --> ing8_Na2S
CHEBI_76208["CHEBI:76208"]
ing8_Na2S -.-> CHEBI_76208
ing9_Na2_EDTA["Na2-EDTA"]
CultureMech_000698 --> ing9_Na2_EDTA
CHEBI_64734["CHEBI:64734"]
ing9_Na2_EDTA -.-> CHEBI_64734
ing10_FeSO4_x_7_H2O["FeSO4 x 7 H2O"]
CultureMech_000698 --> ing10_FeSO4_x_7_H2O
CHEBI_75836["CHEBI:75836"]
ing10_FeSO4_x_7_H2O -.-> CHEBI_75836
ing11_CoCl2_x_6_H2O["CoCl2 x 6 H2O"]
CultureMech_000698 --> ing11_CoCl2_x_6_H2O
CHEBI_53503["CHEBI:53503"]
ing11_CoCl2_x_6_H2O -.-> CHEBI_53503
ing12_MnCl2_x_2_H2O["MnCl2 x 2 H2O"]
CultureMech_000698 --> ing12_MnCl2_x_2_H2O
CHEBI_131395["CHEBI:131395"]
ing12_MnCl2_x_2_H2O -.-> CHEBI_131395
ing13_ZnCl2["ZnCl2"]
CultureMech_000698 --> ing13_ZnCl2
CHEBI_49976["CHEBI:49976"]
ing13_ZnCl2 -.-> CHEBI_49976
ing14_NiCl2_x_6_H2O["NiCl2 x 6 H2O"]
CultureMech_000698 --> ing14_NiCl2_x_6_H2O
CHEBI_34887["CHEBI:34887"]
ing14_NiCl2_x_6_H2O -.-> CHEBI_34887
ing15_Na2MoO4_x_2_H2O["Na2MoO4 x 2 H2O"]
CultureMech_000698 --> ing15_Na2MoO4_x_2_H2O
CHEBI_75213["CHEBI:75213"]
ing15_Na2MoO4_x_2_H2O -.-> CHEBI_75213
ing16_H3BO3["H3BO3"]
CultureMech_000698 --> ing16_H3BO3
CHEBI_33118["CHEBI:33118"]
ing16_H3BO3 -.-> CHEBI_33118
ing17_CuCl2_x_2_H2O["CuCl2 x 2 H2O"]
CultureMech_000698 --> ing17_CuCl2_x_2_H2O
CHEBI_86318["CHEBI:86318"]
ing17_CuCl2_x_2_H2O -.-> CHEBI_86318
Ingredients (18)
| # | Ingredient | Concentration | Ontology | Notes |
|---|---|---|---|---|
| 1 |
NaHCO3
CHO3.Na 84.006 g/mol
|
30 G_PER_L |
CHEBI:32139
sodium hydrogencarbonate
|
|
| 2 |
NaCl
Cl.Na 58.443 g/mol
|
6 G_PER_L |
CHEBI:26710
sodium chloride
|
|
| 3 |
K2HPO4
HO4P.2K 174.174 g/mol
|
0.5 G_PER_L |
CHEBI:131527
dipotassium hydrogen phosphate
|
|
| 4 |
Na2CO3
CO3.2Na 105.988 g/mol
|
5.3 G_PER_L |
CHEBI:29377
sodium carbonate
|
|
| 5 |
MgSO4
Mg.O4S 120.368 g/mol
|
0.12037 G_PER_L |
CHEBI:32599
magnesium sulfate
|
|
| 6 | Yeast extract | 0.05 G_PER_L | ||
| 7 |
Sodium acetate
C2H3O2.Na 82.034 g/mol
|
1.6406 G_PER_L |
CHEBI:32954
sodium acetate
|
|
| 8 |
KNO3
K.NO3 101.102 g/mol
|
2.02206 G_PER_L |
CHEBI:63043
potassium nitrate
|
|
| 9 |
Na2S
2Na.S 78.047 g/mol
|
0.007906 G_PER_L |
CHEBI:76208
sodium sulfide (anhydrous)
|
|
| 10 |
Na2-EDTA
C10H14N2O8.2Na 336.208 g/mol
|
3 G_PER_L |
CHEBI:64734
EDTA disodium salt (anhydrous)
|
|
| 11 |
FeSO4 x 7 H2O
Fe.7H2O.O4S 278.013 g/mol
|
1.1 G_PER_L |
CHEBI:75836
iron(2+) sulfate heptahydrate
|
|
| 12 |
CoCl2 x 6 H2O
2Cl.Co.6H2O 237.929 g/mol
|
0.19 G_PER_L |
CHEBI:53503
cobalt chloride hexahydrate
|
|
| 13 |
MnCl2 x 2 H2O
Cl2Mn.2H2O 161.874 g/mol
|
0.05 G_PER_L |
CHEBI:131395
manganese(II) chloride dihydrate
|
|
| 14 |
ZnCl2
2Cl.Zn 136.296 g/mol
|
0.042 G_PER_L |
CHEBI:49976
zinc dichloride
|
|
| 15 |
NiCl2 x 6 H2O
Cl2Ni 129.599 g/mol
|
0.024 G_PER_L |
CHEBI:34887
nickel dichloride
|
|
| 16 |
Na2MoO4 x 2 H2O
2H2O.MoO4.2Na 241.946 g/mol
|
0.018 G_PER_L |
CHEBI:75213
sodium molybdate dihydrate
|
|
| 17 |
H3BO3
H3BO3 61.833 g/mol
|
0.3 G_PER_L |
CHEBI:33118
boric acid
|
|
| 18 |
CuCl2 x 2 H2O
Cl2Cu.2H2O 170.482 g/mol
|
0.002 G_PER_L |
CHEBI:86318
copper(II) chloride dihydrate
|
Preparation
- Flush the medium with N2 and dispense 20 ml into 100ml serum bottles under an N2 atmosphere, seal with butyl rubber stoppers and autoclave. To the cooled. sterile medium add, from anaerobic, sterile stock solutions, MgSO4 (1 mM), yeast extract (50 mg/l), sodium acetate (20 mM), KNO3 (20 mM) and Na2S (0.1 mM).
- Adjust pH to 6.0.
Target organisms
No target organisms are recorded.
Provenance
| Timestamp | Curator | Action | Notes |
|---|---|---|---|
| 2026-01-26T17:53:13.818389Z | mediadive-import | Imported from MediaDive | Source: DSMZ, ID: 1239 |
| 2026-03-13T06:18:57.563910Z | culturemech-id-assigner-v1.0 | Assigned CultureMech ID | Assigned stable identifier: CultureMech:000698 |
| 2026-03-17T06:30:46.000000Z | mediaingredientmech-enrichment-v1.0 | Added MediaIngredientMech links | Linked ingredients to MediaIngredientMech identifiers |
| 2026-03-17T19:45:08.864958+00:00 | normalize_media_names_to_snake_case.py | NAME_NORMALIZED | Normalized name from "DESULFURISPIRILLUM MEDIUM" to "desulfurispirillum_medium" for consistent matching |
| 2026-06-05T07:40:58.243750+00:00 | mim-legacy-to-chebi-migration-v1.0 | Refreshed legacy MediaIngredientMech links to CHEBI keying | Replaced 15 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme. |
| 2026-06-10T07:37:34.717696+00:00 | primary-term-regrounding-v1.0 | Re-grounded wrong primary term id (OAK-verified) | 1 primary term(s); ingredient name resolved by OAK sqlite:obo:chebi. |
| 2026-06-10T07:46:56.206128+00:00 | primary-term-label-canonicalization-v1.0 | Canonicalized primary term label to OBO canonical (OAK) | 5 primary term(s); ingredient name resolved by OAK sqlite:obo:chebi. |