Overview
- External ontology
- mediadive.medium:1242 (source link unavailable) — ALGORIPHAGUS ALKALIPHILUS MEDIUM
- Applications
-
- Microbial cultivation
- pH range
- {'min': 7.8, 'max': 8.2}
- Notes
- Source: DSMZ | Link: https://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium1242.pdf
Composition
flowchart LR
CultureMech_000701["algoriphagus_alkaliphilus_medium"]
ing0_Yeast_extract["Yeast extract"]
CultureMech_000701 --> ing0_Yeast_extract
ing1_Proteose_peptone_no__3["Proteose peptone no. 3"]
CultureMech_000701 --> ing1_Proteose_peptone_no__3
ing2_Casamino_acids["Casamino acids"]
CultureMech_000701 --> ing2_Casamino_acids
ing3_Glucose["Glucose"]
CultureMech_000701 --> ing3_Glucose
CHEBI_17234["CHEBI:17234"]
ing3_Glucose -.-> CHEBI_17234
ing4_Pyruvic_acid["Pyruvic acid"]
CultureMech_000701 --> ing4_Pyruvic_acid
CHEBI_32816["CHEBI:32816"]
ing4_Pyruvic_acid -.-> CHEBI_32816
ing5_MgSO4_x_7_H2O["MgSO4 x 7 H2O"]
CultureMech_000701 --> ing5_MgSO4_x_7_H2O
CHEBI_31795["CHEBI:31795"]
ing5_MgSO4_x_7_H2O -.-> CHEBI_31795
ing6_K2HPO4["K2HPO4"]
CultureMech_000701 --> ing6_K2HPO4
CHEBI_131527["CHEBI:131527"]
ing6_K2HPO4 -.-> CHEBI_131527
ing7_Tris_buffer["Tris buffer"]
CultureMech_000701 --> ing7_Tris_buffer
CHEBI_9754["CHEBI:9754"]
ing7_Tris_buffer -.-> CHEBI_9754
ing8_Agar["Agar"]
CultureMech_000701 --> ing8_Agar
CHEBI_2509["CHEBI:2509"]
ing8_Agar -.-> CHEBI_2509
ing9_Nitrilotriacetic_acid["Nitrilotriacetic acid"]
CultureMech_000701 --> ing9_Nitrilotriacetic_acid
CHEBI_44557["CHEBI:44557"]
ing9_Nitrilotriacetic_acid -.-> CHEBI_44557
ing10_CaSO4_x_2_H2O["CaSO4 x 2 H2O"]
CultureMech_000701 --> ing10_CaSO4_x_2_H2O
CHEBI_32583["CHEBI:32583"]
ing10_CaSO4_x_2_H2O -.-> CHEBI_32583
ing11_NaCl["NaCl"]
CultureMech_000701 --> ing11_NaCl
CHEBI_26710["CHEBI:26710"]
ing11_NaCl -.-> CHEBI_26710
ing12_KNO3["KNO3"]
CultureMech_000701 --> ing12_KNO3
CHEBI_63043["CHEBI:63043"]
ing12_KNO3 -.-> CHEBI_63043
ing13_NaNO3["NaNO3"]
CultureMech_000701 --> ing13_NaNO3
CHEBI_63005["CHEBI:63005"]
ing13_NaNO3 -.-> CHEBI_63005
ing14_Na2HPO4["Na2HPO4"]
CultureMech_000701 --> ing14_Na2HPO4
CHEBI_34683["CHEBI:34683"]
ing14_Na2HPO4 -.-> CHEBI_34683
ing15_MnSO4_x_H2O["MnSO4 x H2O"]
CultureMech_000701 --> ing15_MnSO4_x_H2O
CHEBI_86364["CHEBI:86364"]
ing15_MnSO4_x_H2O -.-> CHEBI_86364
ing16_ZnSO4_x_7_H2O["ZnSO4 x 7 H2O"]
CultureMech_000701 --> ing16_ZnSO4_x_7_H2O
CHEBI_32312["CHEBI:32312"]
ing16_ZnSO4_x_7_H2O -.-> CHEBI_32312
ing17_H3BO3["H3BO3"]
CultureMech_000701 --> ing17_H3BO3
CHEBI_33118["CHEBI:33118"]
ing17_H3BO3 -.-> CHEBI_33118
ing18_CuSO4_x_5_H2O["CuSO4 x 5 H2O"]
CultureMech_000701 --> ing18_CuSO4_x_5_H2O
CHEBI_31440["CHEBI:31440"]
ing18_CuSO4_x_5_H2O -.-> CHEBI_31440
ing19_Na2MoO4_x_2_H2O["Na2MoO4 x 2 H2O"]
CultureMech_000701 --> ing19_Na2MoO4_x_2_H2O
CHEBI_75213["CHEBI:75213"]
ing19_Na2MoO4_x_2_H2O -.-> CHEBI_75213
ing20_CoCl2_x_6_H2O["CoCl2 x 6 H2O"]
CultureMech_000701 --> ing20_CoCl2_x_6_H2O
CHEBI_53503["CHEBI:53503"]
ing20_CoCl2_x_6_H2O -.-> CHEBI_53503
Ingredients (21)
| # | Ingredient | Concentration | Ontology | Notes |
|---|---|---|---|---|
| 1 | Yeast extract | 1 G_PER_L | ||
| 2 | Proteose peptone no. 3 | 1 G_PER_L | ||
| 3 | Casamino acids | 1 G_PER_L | ||
| 4 |
Glucose
C6H12O6 180.156 g/mol
|
1 G_PER_L |
CHEBI:17234
glucose
|
|
| 5 |
Pyruvic acid
C3H4O3 88.062 g/mol
|
0.05 G_PER_L |
CHEBI:32816
pyruvic acid
|
|
| 6 |
MgSO4 x 7 H2O
7H2O.Mg.O4S 246.473 g/mol
|
1.1 G_PER_L |
CHEBI:31795
magnesium sulfate heptahydrate
|
[Merged 2 duplicates: 0.1, 1.0] |
| 7 |
K2HPO4
HO4P.2K 174.174 g/mol
|
0.6 G_PER_L |
CHEBI:131527
dipotassium hydrogen phosphate
|
|
| 8 |
Tris buffer
C4H11NO3 121.136 g/mol
|
12.114 G_PER_L |
CHEBI:9754
tris
|
|
| 9 | Agar | 15 G_PER_L |
CHEBI:2509
agar
|
|
| 10 |
Nitrilotriacetic acid
C6H9NO6 191.139 g/mol
|
1 G_PER_L |
CHEBI:44557
nitrilotriacetic acid
|
|
| 11 |
CaSO4 x 2 H2O
Ca.2H2O.O4S 172.171 g/mol
|
0.6 G_PER_L |
CHEBI:32583
calcium sulfate dihydrate
|
|
| 12 |
NaCl
Cl.Na 58.443 g/mol
|
0.8 G_PER_L |
CHEBI:26710
sodium chloride
|
|
| 13 |
KNO3
K.NO3 101.102 g/mol
|
1.03 G_PER_L |
CHEBI:63043
potassium nitrate
|
|
| 14 |
NaNO3
NO3.Na 84.994 g/mol
|
6.89 G_PER_L |
CHEBI:63005
sodium nitrate
|
|
| 15 |
Na2HPO4
HO4P.2Na 141.958 g/mol
|
1.11 G_PER_L |
CHEBI:34683
disodium hydrogenphosphate
|
|
| 16 |
MnSO4 x H2O
H2O.Mn.O4S 169.016 g/mol
|
0.22 G_PER_L |
CHEBI:86364
manganese(II) sulfate monohydrate
|
|
| 17 |
ZnSO4 x 7 H2O
7H2O.O4S.Zn 287.558 g/mol
|
0.05 G_PER_L |
CHEBI:32312
zinc sulfate heptahydrate
|
|
| 18 |
H3BO3
H3BO3 61.833 g/mol
|
0.05 G_PER_L |
CHEBI:33118
boric acid
|
|
| 19 |
CuSO4 x 5 H2O
Cu.5H2O.O4S 249.684 g/mol
|
0.0025 G_PER_L |
CHEBI:31440
copper(II) sulfate pentahydrate
|
|
| 20 |
Na2MoO4 x 2 H2O
2H2O.MoO4.2Na 241.946 g/mol
|
0.0025 G_PER_L |
CHEBI:75213
sodium molybdate dihydrate
|
|
| 21 |
CoCl2 x 6 H2O
2Cl.Co.6H2O 237.929 g/mol
|
0.0046 G_PER_L |
CHEBI:53503
cobalt chloride hexahydrate
|
Preparation
- Adjust pH 7.8-8.2
- Autoclave at 121°C for 15 minutes.
Target organisms
No target organisms are recorded.
Provenance
| Timestamp | Curator | Action | Notes |
|---|---|---|---|
| 2026-01-26T17:53:13.826810Z | mediadive-import | Imported from MediaDive | Source: DSMZ, ID: 1242 |
| 2026-01-28T19:48:33.255800Z | chebi-enrichment | Added CHEBI ontology terms to ingredients | Enriched using MicrobeMediaParam and MediaDive chemical mappings |
| 2026-03-13T03:09:26.578944Z | data-quality-cleanup-v1.0 | Data quality fixes | Merged 1 duplicate ingredient(s) |
| 2026-03-13T06:18:57.584988Z | culturemech-id-assigner-v1.0 | Assigned CultureMech ID | Assigned stable identifier: CultureMech:000701 |
| 2026-03-17T06:30:35.000000Z | mediaingredientmech-enrichment-v1.0 | Added MediaIngredientMech links | Linked ingredients to MediaIngredientMech identifiers |
| 2026-03-17T19:45:08.889414+00:00 | normalize_media_names_to_snake_case.py | NAME_NORMALIZED | Normalized name from "ALGORIPHAGUS ALKALIPHILUS MEDIUM" to "algoriphagus_alkaliphilus_medium" for consistent matching |
| 2026-05-20T06:01:07.743619+00:00 | migrate_ph_range.py | MIGRATED_PH_RANGE | ph_range '7.8-8.2' -> {'min': 7.8, 'max': 8.2} |
| 2026-06-05T07:40:47.714435+00:00 | mim-legacy-to-chebi-migration-v1.0 | Refreshed legacy MediaIngredientMech links to CHEBI keying | Replaced 17 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme. |
| 2026-06-08T23:19:35.538437+00:00 | chebi-regrounding-v1.0 | Corrected CHEBI ingredient grounding (audit G21/G22) | Re-grounded 2 term reference(s) to the correct CHEBI (see reports/chebi_grounding_audit.md). Label-conditional fixes for pyridoxine/pyridoxamine, glycerol, MnSO4, Ca(NO3)2, selenate; casamino acids de-grounded (mixture, no single CHEBI). |
| 2026-06-10T07:37:34.489382+00:00 | primary-term-regrounding-v1.0 | Re-grounded wrong primary term id (OAK-verified) | 1 primary term(s); ingredient name resolved by OAK sqlite:obo:chebi. |
| 2026-06-10T07:46:52.735636+00:00 | primary-term-label-canonicalization-v1.0 | Canonicalized primary term label to OBO canonical (OAK) | 3 primary term(s); ingredient name resolved by OAK sqlite:obo:chebi. |