oteb_medium

CultureMech:000704 bacterial COMPLEX LIQUID
Original name: OTEB-MEDIUM

Overview

External ontology
mediadive.medium:1245 (source link unavailable) — OTEB-MEDIUM
Applications
  • Microbial cultivation
pH range
{'min': 6.8, 'max': 7.2}
Notes
Source: DSMZ | Link: https://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium1245.pdf Superseded historical provenance, not a current formulation claim: Commercial Product: LB Medium (Luria-Bertani, Miller formulation) Supplier: Multiple suppliers (Difco, Sigma, etc.) Catalog: Multiple (L3522 Sigma, 244520 BD) Source: https://www.laboratorynotes.com/preparation-of-luria-bertani-lb-miller-broth/ Accessed: 2026-03-28 Constituent composition researched from product specification. Preserve source descriptive/preparation text without falsely encoding every paragraph as an instruction: OTEB-medium is available from Anaerobe Systems (http://www.anaerobesystems.com) The following text was taken from the above page: - ORAL TREPONEME ENRICHMENT BROTH (OTEB) - Products: AS-603 Oral Treponeme Enrichment Broth (OTEB) 10 tubes / pkg Intended Use: OTEB is an enriched medium for the isolation, cultivation and maintenance of Treponemes found as part of the oral flora. OTEB is a complex peptone-yeast extract medium containing volatile fatty acids and serum for a source of long chain fatty acids. Volatile fatty acids and serum are necessary nutrients for recovery of Treponemes from the oral flora. Inoculation of the medium can be done using a gassing cannula or inside an anaerobic chamber. This medium is prepared, stored and dispensed under oxygen-free conditions to prevent the formation of oxidized products prior to use. Final pH 7.0 +/- 0.2 at 25 degrees C. Final volume 7.0 ml +/- 0.7. The original states final pH 7.0 +/- 0.2 at 25 C: the range 6.8-7.2 remains, while 25 C is a measurement condition. The printed final volume 7.0 +/- 0.7 ml is retained, but cannot establish this formula's batch denominator. The API declares 1000 ml although listed liquid inputs sum to 1003.1 ml. No final-medium g/L or density conversion is inferred. Complete source tables were inspected. Reconstructed ingredient/stock nodes retain source-specific amounts and uncertainties; historical flattened amounts, false supplier-tagged LB ingredients and unsupported interpretations are archived in append-only history. Existing unrelated metadata and prior evidence remain preserved, not recertified.

Composition

flowchart LR
    CultureMech_000704["oteb_medium"]
    ing0_Proteose_peptone_no__2["Proteose peptone no. 2"]
    CultureMech_000704 --> ing0_Proteose_peptone_no__2
    ing1_Heart_Infusion_Broth["Heart Infusion Broth"]
    CultureMech_000704 --> ing1_Heart_Infusion_Broth
    ing2_Yeast_extract["Yeast extract"]
    CultureMech_000704 --> ing2_Yeast_extract
    ing3_Potassium_Phosphate__dibasic["Potassium Phosphate, dibasic"]
    CultureMech_000704 --> ing3_Potassium_Phosphate__dibasic
    ing4_Sodium_Chloride["Sodium Chloride"]
    CultureMech_000704 --> ing4_Sodium_Chloride
    CHEBI_26710["CHEBI:26710"]
    ing4_Sodium_Chloride -.-> CHEBI_26710
    ing5_Magnesium_sulfate["Magnesium sulfate"]
    CultureMech_000704 --> ing5_Magnesium_sulfate
    CHEBI_32599["CHEBI:32599"]
    ing5_Magnesium_sulfate -.-> CHEBI_32599
    ing6_Acetic_acid["Acetic acid"]
    CultureMech_000704 --> ing6_Acetic_acid
    CHEBI_15366["CHEBI:15366"]
    ing6_Acetic_acid -.-> CHEBI_15366
    ing7_Propionic_acid["Propionic acid"]
    CultureMech_000704 --> ing7_Propionic_acid
    CHEBI_30768["CHEBI:30768"]
    ing7_Propionic_acid -.-> CHEBI_30768
    ing8_n_Butyric_acid["n-Butyric acid"]
    CultureMech_000704 --> ing8_n_Butyric_acid
    CHEBI_30772["CHEBI:30772"]
    ing8_n_Butyric_acid -.-> CHEBI_30772
    ing9_n_Valeric_acid["n-Valeric acid"]
    CultureMech_000704 --> ing9_n_Valeric_acid
    CHEBI_17418["CHEBI:17418"]
    ing9_n_Valeric_acid -.-> CHEBI_17418
    ing10_Isovaleric_acid["Isovaleric acid"]
    CultureMech_000704 --> ing10_Isovaleric_acid
    CHEBI_28484["CHEBI:28484"]
    ing10_Isovaleric_acid -.-> CHEBI_28484
    ing11_Isobutyric_acid["Isobutyric acid"]
    CultureMech_000704 --> ing11_Isobutyric_acid
    CHEBI_16135["CHEBI:16135"]
    ing11_Isobutyric_acid -.-> CHEBI_16135
    ing12_2_Methylbutyric_acid["2-Methylbutyric acid"]
    CultureMech_000704 --> ing12_2_Methylbutyric_acid
    ing13_L_Cysteine["L-Cysteine"]
    CultureMech_000704 --> ing13_L_Cysteine
    CHEBI_17561["CHEBI:17561"]
    ing13_L_Cysteine -.-> CHEBI_17561
    ing14_Calf_Serum__inactivated["Calf Serum, inactivated"]
    CultureMech_000704 --> ing14_Calf_Serum__inactivated
    ing15_Distilled_water["Distilled water"]
    CultureMech_000704 --> ing15_Distilled_water

Ingredients (16)

# Ingredient Concentration Ontology Notes
1 Proteose peptone no. 2 Original recipe input only. No inferred denominator, density, percentage basis, salt form, or receiving-medium dilution. Preserve repeated source rows separately. Original main row 1: 5.0 g per preparation. Mass is retained in notes because the source provides no defensible final-volume denominator for this preparation; the API's g_l is not recertified as an original measured concentration.
2 Heart Infusion Broth Original recipe input only. No inferred denominator, density, percentage basis, salt form, or receiving-medium dilution. Preserve repeated source rows separately. Original main row 2: 5.0 g per preparation. Mass is retained in notes because the source provides no defensible final-volume denominator for this preparation; the API's g_l is not recertified as an original measured concentration.
3 Yeast extract Original recipe input only. No inferred denominator, density, percentage basis, salt form, or receiving-medium dilution. Preserve repeated source rows separately. Original main row 3: 5.0 g per preparation. Mass is retained in notes because the source provides no defensible final-volume denominator for this preparation; the API's g_l is not recertified as an original measured concentration.
4 Potassium Phosphate, dibasic (dibasic) Original recipe input only. No inferred denominator, density, percentage basis, salt form, or receiving-medium dilution. Preserve repeated source rows separately. Original main row 4: 2.0 g per preparation. Mass is retained in notes because the source provides no defensible final-volume denominator for this preparation; the API's g_l is not recertified as an original measured concentration. The copied monobasic or chloride-specific annotation does not establish the source's stated form. Withdraw it without inventing a new identifier.
5 Sodium Chloride
Cl.Na 58.443 g/mol
CHEBI:26710
sodium chloride
 Original recipe input only. No inferred denominator, density, percentage basis, salt form, or receiving-medium dilution. Preserve repeated source rows separately. Original main row 5: 5.0 g per preparation. Mass is retained in notes because the source provides no defensible final-volume denominator for this preparation; the API's g_l is not recertified as an original measured concentration.
6 Magnesium sulfate
Mg.O4S 120.368 g/mol
CHEBI:32599
magnesium sulfate
 Original recipe input only. No inferred denominator, density, percentage basis, salt form, or receiving-medium dilution. Preserve repeated source rows separately. Original main row 6: 0.1 g per preparation. Mass is retained in notes because the source provides no defensible final-volume denominator for this preparation; the API's g_l is not recertified as an original measured concentration.
7 Acetic acid
C2H4O2 60.052 g/mol
0.0016 L CHEBI:15366
acetic acid
 Original recipe input only. No inferred denominator, density, percentage basis, salt form, or receiving-medium dilution. Preserve repeated source rows separately. Original main row 7: 1.6 ml per preparation.
8 Propionic acid
C3H6O2 74.079 g/mol
0.0006 L CHEBI:30768
propionic acid
 Original recipe input only. No inferred denominator, density, percentage basis, salt form, or receiving-medium dilution. Preserve repeated source rows separately. Original main row 8: 0.6 ml per preparation.
9 n-Butyric acid
C4H8O2 88.106 g/mol
0.0004 L CHEBI:30772
butyric acid
 Original recipe input only. No inferred denominator, density, percentage basis, salt form, or receiving-medium dilution. Preserve repeated source rows separately. Original main row 9: 0.4 ml per preparation.
10 n-Valeric acid
C5H10O2 102.133 g/mol
0.0001 L CHEBI:17418
valeric acid
 Original recipe input only. No inferred denominator, density, percentage basis, salt form, or receiving-medium dilution. Preserve repeated source rows separately. Original main row 10: 0.1 ml per preparation.
11 Isovaleric acid
C5H10O2 102.133 g/mol
0.0001 L CHEBI:28484
isovaleric acid
 Original recipe input only. No inferred denominator, density, percentage basis, salt form, or receiving-medium dilution. Preserve repeated source rows separately. Original main row 11: 0.1 ml per preparation.
12 Isobutyric acid
C4H8O2 88.106 g/mol
0.0001 L CHEBI:16135
isobutyric acid
 Original recipe input only. No inferred denominator, density, percentage basis, salt form, or receiving-medium dilution. Preserve repeated source rows separately. Original main row 12: 0.1 ml per preparation.
13 2-Methylbutyric acid 0.0001 L Original recipe input only. No inferred denominator, density, percentage basis, salt form, or receiving-medium dilution. Preserve repeated source rows separately. Original main row 13: 0.1 ml per preparation.
14 L-Cysteine
C3H7NO2S 121.161 g/mol
CHEBI:17561
L-cysteine
 Original recipe input only. No inferred denominator, density, percentage basis, salt form, or receiving-medium dilution. Preserve repeated source rows separately. Original main row 15: 0.5 g per preparation. Mass is retained in notes because the source provides no defensible final-volume denominator for this preparation; the API's g_l is not recertified as an original measured concentration.
15 Calf Serum, inactivated 0.1 L (inactivated) Original recipe input only. No inferred denominator, density, percentage basis, salt form, or receiving-medium dilution. Preserve repeated source rows separately. Retain the original label and expose the API fetal-bovine-serum disagreement. Original main row 16: 100.0 ml per preparation. Identity conflict: the original says inactivated calf serum; the API says Fetal bovine serum. This record retains the original label without asserting biological interchangeability.
16 Distilled water 0.8 L Original recipe input only. No inferred denominator, density, percentage basis, salt form, or receiving-medium dilution. Preserve repeated source rows separately. Original main row 18: 800.0 ml per preparation.

Solutions

Vitamin K1 (1% solution)

Dose: 0.0001 L

Stock composition
IngredientConcentrationNotes
Vitamin K1 The original specifies 1% soln but does not identify w/v, w/w, or v/v. Keep that literal strength in notes, without asserting a numeric concentration or stock solvent.
Original recipe input only. No inferred denominator, density, percentage basis, salt form, or receiving-medium dilution. Preserve repeated source rows separately. The amount is 0.1 ml of a stock whose 1% basis is unresolved, not vitamin-K1 mass. Original main row 14: 0.1 ml per preparation.

Sugar Stock Solution

Dose: 0.1 L

Stock composition
IngredientConcentrationNotes
Glucose Original recipe input only. No inferred denominator, density, percentage basis, salt form, or receiving-medium dilution. Preserve repeated source rows separately. Original sugar_stock row 1: 8.0 g per preparation. Mass is retained in notes because the source provides no defensible final-volume denominator for this preparation; the API's g_l is not recertified as an original measured concentration.
Fructose Original recipe input only. No inferred denominator, density, percentage basis, salt form, or receiving-medium dilution. Preserve repeated source rows separately. Original sugar_stock row 2: 8.0 g per preparation. Mass is retained in notes because the source provides no defensible final-volume denominator for this preparation; the API's g_l is not recertified as an original measured concentration.
Sucrose Original recipe input only. No inferred denominator, density, percentage basis, salt form, or receiving-medium dilution. Preserve repeated source rows separately. Original sugar_stock row 3: 8.0 g per preparation. Mass is retained in notes because the source provides no defensible final-volume denominator for this preparation; the API's g_l is not recertified as an original measured concentration.
Maltose Superseded flattening history, not a current amount: [Collapsed 2 identical duplicates: 8.0, 8.0] Original recipe input only. No inferred denominator, density, percentage basis, salt form, or receiving-medium dilution. Preserve repeated source rows separately. Original sugar_stock row 4: 8.0 g per preparation. Mass is retained in notes because the source provides no defensible final-volume denominator for this preparation; the API's g_l is not recertified as an original measured concentration. Preserve this distinct literal source row. Whether the two 8 g rows are intentional is unresolved; do not sum or collapse them.
Maltose Superseded flattening history, not a current amount: [Collapsed 2 identical duplicates: 8.0, 8.0] Original recipe input only. No inferred denominator, density, percentage basis, salt form, or receiving-medium dilution. Preserve repeated source rows separately. Original sugar_stock row 5: 8.0 g per preparation. Mass is retained in notes because the source provides no defensible final-volume denominator for this preparation; the API's g_l is not recertified as an original measured concentration. Preserve this distinct literal source row. Whether the two 8 g rows are intentional is unresolved; do not sum or collapse them.
Ribose Original recipe input only. No inferred denominator, density, percentage basis, salt form, or receiving-medium dilution. Preserve repeated source rows separately. Original sugar_stock row 6: 8.0 g per preparation. Mass is retained in notes because the source provides no defensible final-volume denominator for this preparation; the API's g_l is not recertified as an original measured concentration.
Xylose Original recipe input only. No inferred denominator, density, percentage basis, salt form, or receiving-medium dilution. Preserve repeated source rows separately. Original sugar_stock row 7: 8.0 g per preparation. Mass is retained in notes because the source provides no defensible final-volume denominator for this preparation; the API's g_l is not recertified as an original measured concentration.
Pectin Original recipe input only. No inferred denominator, density, percentage basis, salt form, or receiving-medium dilution. Preserve repeated source rows separately. Original sugar_stock row 8: 8.0 g per preparation. Mass is retained in notes because the source provides no defensible final-volume denominator for this preparation; the API's g_l is not recertified as an original measured concentration.
Soluble Starch Original recipe input only. No inferred denominator, density, percentage basis, salt form, or receiving-medium dilution. Preserve repeated source rows separately. Original sugar_stock row 9: 8.0 g per preparation. Mass is retained in notes because the source provides no defensible final-volume denominator for this preparation; the API's g_l is not recertified as an original measured concentration.
Sodium pyruvate Original recipe input only. No inferred denominator, density, percentage basis, salt form, or receiving-medium dilution. Preserve repeated source rows separately. Original sugar_stock row 10: 8.0 g per preparation. Mass is retained in notes because the source provides no defensible final-volume denominator for this preparation; the API's g_l is not recertified as an original measured concentration.
Thiamine pyrophosphate Original recipe input only. No inferred denominator, density, percentage basis, salt form, or receiving-medium dilution. Preserve repeated source rows separately. Original sugar_stock row 11: 750.0 μg per preparation. Mass is retained in notes because the source provides no defensible final-volume denominator for this preparation; the API's g_l is not recertified as an original measured concentration. The copied monobasic or chloride-specific annotation does not establish the source's stated form. Withdraw it without inventing a new identifier.
Distilled water 1 L Original recipe input only. No inferred denominator, density, percentage basis, salt form, or receiving-medium dilution. Preserve repeated source rows separately. Original sugar_stock row 12: 1000.0 ml per preparation.
Original recipe input only. No inferred denominator, density, percentage basis, salt form, or receiving-medium dilution. Preserve repeated source rows separately. This stock addition is separate from its twelve composition rows. Original main row 17: 100.0 ml per preparation.

Target organisms

No target organisms are recorded.

Provenance

TimestampCuratorActionNotes
2026-01-26T17:53:13.839737Z mediadive-import Imported from MediaDive Source: DSMZ, ID: 1245
2026-03-13T03:09:35.969529Z data-quality-cleanup-v1.0 Data quality fixes Merged 1 duplicate ingredient(s)
2026-03-13T06:18:57.620952Z culturemech-id-assigner-v1.0 Assigned CultureMech ID Assigned stable identifier: CultureMech:000704
2026-03-17T06:30:47.000000Z mediaingredientmech-enrichment-v1.0 Added MediaIngredientMech links Linked ingredients to MediaIngredientMech identifiers
2026-03-17T19:45:08.929200+00:00 normalize_media_names_to_snake_case.py NAME_NORMALIZED Normalized name from "OTEB-MEDIUM" to "oteb_medium" for consistent matching
2026-05-20T06:02:01.671640+00:00 migrate_ph_range.py MIGRATED_PH_RANGE ph_range '6.8-7.2' -> {'min': 6.8, 'max': 7.2}
2026-06-05T07:41:40.660657+00:00 mim-legacy-to-chebi-migration-v1.0 Refreshed legacy MediaIngredientMech links to CHEBI keying Replaced 21 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme.
2026-09-02T07:38:54.521282+00:00 repair_merged_duplicates.py REPAIRED_SUMMED_DUPLICATE_MERGE Collapsed 1 ingredient row(s) whose concentration was the SUM of identical duplicate values written by cleanup_media_quality.py. Every merged part was the same value, so the pre-merge value is unambiguous and no source lookup was needed. Rows whose merged parts differ were left untouched.
2026-10-09T06:18:47.388455+00:00 codex RESTORED_OTEB_SOURCE_FORMULATIONS_AND_STOCK_SCOPES_WITH_EVIDENCE Restore exact-source OTEB formulation and stock scopes, preserve provider rates/nulls and original source conflicts, correct input units and family links, and retain prior evidence/history. No biological growth association added.