nutriment_agar_with_150_g_l_nacl

CultureMech:000716 bacterial COMPLEX SOLID_AGAR
Original name: NUTRIMENT AGAR WITH 150 G/L NACL

Overview

External ontology
mediadive.medium:1258 (source link unavailable) — NUTRIMENT AGAR WITH 150 G/L NACL
Applications
  • Microbial cultivation
pH
7.2
Notes
Source: DSMZ | Link: https://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium1258.pdf

Composition

flowchart LR
    CultureMech_000716["nutriment_agar_with_150_g_l_nacl"]
    ing0_Peptone["Peptone"]
    CultureMech_000716 --> ing0_Peptone
    ing1_Meat_extract["Meat extract"]
    CultureMech_000716 --> ing1_Meat_extract
    ing2_Yeast_extract["Yeast extract"]
    CultureMech_000716 --> ing2_Yeast_extract
    ing3_NaCl["NaCl"]
    CultureMech_000716 --> ing3_NaCl
    CHEBI_26710["CHEBI:26710"]
    ing3_NaCl -.-> CHEBI_26710
    ing4_Agar["Agar"]
    CultureMech_000716 --> ing4_Agar
    CHEBI_2509["CHEBI:2509"]
    ing4_Agar -.-> CHEBI_2509

Ingredients (5)

# Ingredient Concentration Ontology Notes
1 Peptone 5 G_PER_L MICRO:0000178 (source link unavailable)
Peptone
2 Meat extract 1 G_PER_L
3 Yeast extract 2 G_PER_L FOODON:03315426
Yeast extract
4 NaCl
Cl.Na 58.443 g/mol
150 G_PER_L CHEBI:26710
sodium chloride
5 Agar 20 G_PER_L CHEBI:2509
agar

Preparation

  1. Adjust to pH 7.2
  2. Autoclave at 121°C 15 min.

Target organisms

No target organisms are recorded.

Provenance

TimestampCuratorActionNotes
2026-01-26T17:53:13.879695Z mediadive-import Imported from MediaDive Source: DSMZ, ID: 1258
2026-03-13T06:18:57.738111Z culturemech-id-assigner-v1.0 Assigned CultureMech ID Assigned stable identifier: CultureMech:000716
2026-03-17T06:29:53.000000Z mediaingredientmech-enrichment-v1.0 Added MediaIngredientMech links Linked ingredients to MediaIngredientMech identifiers
2026-03-17T19:45:09.055520+00:00 normalize_media_names_to_snake_case.py NAME_NORMALIZED Normalized name from "NUTRIMENT AGAR WITH 150 G/L NACL" to "nutriment_agar_with_150_g_l_nacl" for consistent matching
2026-06-05T07:41:40.256301+00:00 mim-legacy-to-chebi-migration-v1.0 Refreshed legacy MediaIngredientMech links to CHEBI keying Replaced 2 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme.
2026-09-13T00:00:00-07:00 repair_bacterial_score10_exact_terms_batch6.py GROUNDED_BACTERIAL_SCORE10_EXACT_TERMS_BATCH6 Applied local mappings for Peptone, Yeast extract.
2026-10-07T13:32:40.086487+00:00 codex CURATED_MEDIUM_CONCENTRATION_SOURCE_EVIDENCE Exact medium/source membership, ingredient inventory/forms, physical-state conditions, literal snippets, and arithmetic checked. Stock boundaries, source-only rows, preparation context, and variants remain explicit follow-up tasks; secondary chemical mappings unchanged.