methylobacterium_medium (CultureMech:000718)

Overview

External ontology

mediadive.medium:125 (source link unavailable) — METHYLOBACTERIUM MEDIUM

Applications

Microbial cultivation

pH

6.8

Notes

Source: DSMZ | Link: https://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium125.pdf Common basal table for two source-listed alternative preparations: add 5 ml methanol or incubate under methane-air (4:1). Neither alternative is selected or combined here. The retained g/L entries are provider-declared basal-table rates, not independently verified final concentrations after choosing a carbon preparation. The 1000 ml water entry is a batch input, not a measured final volume. Methanol purity, final volume, methanol final concentration and dissolved methane concentration remain unspecified. Existing chemical mappings and strain-modified source-duplicate links are preserved, not newly validated by this recipe evidence.

Composition

flowchart LR
    CultureMech_000718["methylobacterium_medium"]
    ing0_KNO3["KNO3"]
    CultureMech_000718 --> ing0_KNO3
    CHEBI_63043["CHEBI:63043"]
    ing0_KNO3 -.-> CHEBI_63043
    ing1_MgSO4_x_7_H2O["MgSO4 x 7 H2O"]
    CultureMech_000718 --> ing1_MgSO4_x_7_H2O
    CHEBI_31795["CHEBI:31795"]
    ing1_MgSO4_x_7_H2O -.-> CHEBI_31795
    ing2_CaCl2_x_2_H2O["CaCl2 x 2 H2O"]
    CultureMech_000718 --> ing2_CaCl2_x_2_H2O
    CHEBI_86158["CHEBI:86158"]
    ing2_CaCl2_x_2_H2O -.-> CHEBI_86158
    ing3_Na2HPO4["Na2HPO4"]
    CultureMech_000718 --> ing3_Na2HPO4
    CHEBI_34683["CHEBI:34683"]
    ing3_Na2HPO4 -.-> CHEBI_34683
    ing4_NaH2PO4["NaH2PO4"]
    CultureMech_000718 --> ing4_NaH2PO4
    CHEBI_37585["CHEBI:37585"]
    ing4_NaH2PO4 -.-> CHEBI_37585
    ing5_FeSO4_x_7_H2O["FeSO4 x 7 H2O"]
    CultureMech_000718 --> ing5_FeSO4_x_7_H2O
    CHEBI_75836["CHEBI:75836"]
    ing5_FeSO4_x_7_H2O -.-> CHEBI_75836
    ing6_CuSO4_x_5_H2O["CuSO4 x 5 H2O"]
    CultureMech_000718 --> ing6_CuSO4_x_5_H2O
    CHEBI_31440["CHEBI:31440"]
    ing6_CuSO4_x_5_H2O -.-> CHEBI_31440
    ing7_H3BO3["H3BO3"]
    CultureMech_000718 --> ing7_H3BO3
    CHEBI_33118["CHEBI:33118"]
    ing7_H3BO3 -.-> CHEBI_33118
    ing8_MnSO4_x_5_H2O["MnSO4 x 5 H2O"]
    CultureMech_000718 --> ing8_MnSO4_x_5_H2O
    CHEBI_131524["CHEBI:131524"]
    ing8_MnSO4_x_5_H2O -.-> CHEBI_131524
    ing9_ZnSO4_x_7_H2O["ZnSO4 x 7 H2O"]
    CultureMech_000718 --> ing9_ZnSO4_x_7_H2O
    CHEBI_32312["CHEBI:32312"]
    ing9_ZnSO4_x_7_H2O -.-> CHEBI_32312
    ing10_MoO3["MoO3"]
    CultureMech_000718 --> ing10_MoO3
    CHEBI_30627["CHEBI:30627"]
    ing10_MoO3 -.-> CHEBI_30627
    ing11_Agar["Agar"]
    CultureMech_000718 --> ing11_Agar
    CHEBI_2509["CHEBI:2509"]
    ing11_Agar -.-> CHEBI_2509
    ing12_Distilled_water["Distilled water"]
    CultureMech_000718 --> ing12_Distilled_water

Ingredients (13)

#	Ingredient	Concentration	Ontology	Notes
1	KNO3 K.NO3 101.102 g/mol	1 G_PER_L	CHEBI:63043 potassium nitrate
2	MgSO4 x 7 H2O 7H2O.Mg.O4S 246.473 g/mol	0.2 G_PER_L	CHEBI:31795 magnesium sulfate heptahydrate
3	CaCl2 x 2 H2O Ca.2Cl.2H2O 147.014 g/mol	0.02 G_PER_L	CHEBI:86158 calcium chloride dihydrate
4	Na2HPO4 HO4P.2Na 141.958 g/mol	0.23 G_PER_L	CHEBI:34683 disodium hydrogenphosphate
5	NaH2PO4 H2O4P.Na 119.976 g/mol	0.07 G_PER_L	CHEBI:37585 sodium dihydrogenphosphate
6	FeSO4 x 7 H2O Fe.7H2O.O4S 278.013 g/mol	0.001 G_PER_L	CHEBI:75836 iron(2+) sulfate heptahydrate
7	CuSO4 x 5 H2O Cu.5H2O.O4S 249.684 g/mol	5e-06 G_PER_L	CHEBI:31440 copper(II) sulfate pentahydrate
8	H3BO3 H3BO3 61.833 g/mol	1e-05 G_PER_L	CHEBI:33118 boric acid
9	MnSO4 x 5 H2O 5H2O.Mn.O4S 241.076 g/mol	1e-05 G_PER_L	CHEBI:131524 manganese(II) sulfate pentahydrate
10	ZnSO4 x 7 H2O 7H2O.O4S.Zn 287.558 g/mol	7e-05 G_PER_L	CHEBI:32312 zinc sulfate heptahydrate
11	MoO3 MoO3 143.937 g/mol	1e-05 G_PER_L	CHEBI:30627 molybdenum trioxide
12	Agar	12 G_PER_L	CHEBI:2509 agar
13	Distilled water	1 L	mediadive.compound:4 (source link unavailable) Distilled water	Batch distilled-water input: 1000 ml = 1 L; not a percentage or a measured final medium volume.

Preparation

Adjust pH to 6.8.

Target organisms

No target organisms are recorded.

Recipe variants

Name
Methanol preparation option
Relationship
SUPPLEMENTED_VARIANT
Description
Source-listed methanol alternative to methane-air incubation; common basal table unchanged.
Modifications
- Add 5 ml methanol to the source's common basal batch; do not combine this option with the methane-air alternative.
- This is a batch addition, not 5 ml/L or 0.5% final-medium methanol; final volume and methanol purity are not specified.
Purpose
Represent the source-listed methanol preparation without choosing it for every use.
Evidence
- Reference
  https://mediadive.dsmz.de/rest/medium/125
  Supports
  SUPPORT
  Snippet
  Add 5 ml methanol or incubate under methane-air (4:1). Adjust pH to 6.8.
  Explanation
  variants[0] (Methanol preparation option): Source-listed alternatives, not simultaneous components. Common basal table for two source-listed alternative preparations: add 5 ml methanol or incubate under methane-air (4:1). Neither alternative is selected or combined here. The retained g/L entries are provider-declared basal-table rates, not independently verified final concentrations after choosing a carbon preparation. The 1000 ml water entry is a batch input, not a measured final volume. Methanol purity, final volume, methanol final concentration and dissolved methane concentration remain unspecified. Existing chemical mappings and strain-modified source-duplicate links are preserved, not newly validated by this recipe evidence. Locator=$.data.solutions[0].steps[0].step. Retrieved 2026-10-07T10:42:34.501511+00:00; response SHA256=2ccb593505b6c8f5cd63fd63bf1ecee7b33357bca201285eb2e958b70aff5ba1.
Name
Methane-air incubation option
Relationship
DERIVED_FROM
Description
Source-listed incubation alternative to methanol addition; common basal table unchanged.
Modifications
- Incubate under methane-air (4:1) as the alternative to adding methanol.
- The gas ratio is not a liquid ingredient dose or a dissolved methane concentration; no gas pressure or total volume is specified.
Purpose
Represent the source-listed methane-air alternative without asserting a dissolved carbon concentration.
Evidence
- Reference
  https://mediadive.dsmz.de/rest/medium/125
  Supports
  SUPPORT
  Snippet
  Add 5 ml methanol or incubate under methane-air (4:1). Adjust pH to 6.8.
  Explanation
  variants[1] (Methane-air incubation option): Source-listed alternatives, not simultaneous components. Common basal table for two source-listed alternative preparations: add 5 ml methanol or incubate under methane-air (4:1). Neither alternative is selected or combined here. The retained g/L entries are provider-declared basal-table rates, not independently verified final concentrations after choosing a carbon preparation. The 1000 ml water entry is a batch input, not a measured final volume. Methanol purity, final volume, methanol final concentration and dissolved methane concentration remain unspecified. Existing chemical mappings and strain-modified source-duplicate links are preserved, not newly validated by this recipe evidence. Locator=$.data.solutions[0].steps[0].step. Retrieved 2026-10-07T10:42:34.501511+00:00; response SHA256=2ccb593505b6c8f5cd63fd63bf1ecee7b33357bca201285eb2e958b70aff5ba1.

Provenance

Timestamp	Curator	Action	Notes
2026-01-26T17:53:10.259882Z	mediadive-import	Imported from MediaDive	Source: DSMZ, ID: 125
2026-03-13T06:18:57.746970Z	culturemech-id-assigner-v1.0	Assigned CultureMech ID	Assigned stable identifier: CultureMech:000718
2026-03-17T06:30:06.000000Z	mediaingredientmech-enrichment-v1.0	Added MediaIngredientMech links	Linked ingredients to MediaIngredientMech identifiers
2026-03-17T19:45:09.065082+00:00	normalize_media_names_to_snake_case.py	NAME_NORMALIZED	Normalized name from "METHYLOBACTERIUM MEDIUM" to "methylobacterium_medium" for consistent matching
2026-06-05T07:41:33.021631+00:00	mim-legacy-to-chebi-migration-v1.0	Refreshed legacy MediaIngredientMech links to CHEBI keying	Replaced 11 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme.
2026-06-10T07:37:35.504058+00:00	primary-term-regrounding-v1.0	Re-grounded wrong primary term id (OAK-verified)	1 primary term(s); ingredient name resolved by OAK sqlite:obo:chebi.
2026-06-10T07:47:07.517399+00:00	primary-term-label-canonicalization-v1.0	Canonicalized primary term label to OBO canonical (OAK)	2 primary term(s); ingredient name resolved by OAK sqlite:obo:chebi.
2026-10-07T10:55:41.454416+00:00	codex	CURATED_MEDIUM_CONCENTRATION_SOURCE_EVIDENCE	Exact medium/source membership, ingredient inventory/forms, physical-state conditions, literal snippets, and arithmetic checked. Stock boundaries, source-only rows, preparation context, and variants remain explicit follow-up tasks; secondary chemical mappings unchanged.
2026-10-07T22:16:38.387474+00:00	codex	RESTORED_DSMZ125_ALTERNATIVE_CARBON_PREPARATIONS_AND_WATER_INPUT	Preserved basal provider rates and prior evidence. Corrected/restored batch water input and represented alternative carbon preparations without inferring final volume, methane dissolution or methanol concentration. Preserved IDs, chemical mappings, history and source-duplicate links; no growth association added.