isp_2_medium_with_10_nacl (CultureMech:000720)

Overview

External ontology

mediadive.medium:1261 (source link unavailable) — ISP 2 MEDIUM WITH 10% NACL

Applications

Microbial cultivation

pH range

{'min': 7.3, 'max': 7.5}

Notes

Source: DSMZ | Link: https://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium1261.pdf

Composition

flowchart LR
    CultureMech_000720["isp_2_medium_with_10_nacl"]
    ing0_Yeast_extract["Yeast extract"]
    CultureMech_000720 --> ing0_Yeast_extract
    ing1_Malt_extract["Malt extract"]
    CultureMech_000720 --> ing1_Malt_extract
    ing2_Glucose["Glucose"]
    CultureMech_000720 --> ing2_Glucose
    CHEBI_17234["CHEBI:17234"]
    ing2_Glucose -.-> CHEBI_17234
    ing3_NaCl["NaCl"]
    CultureMech_000720 --> ing3_NaCl
    CHEBI_26710["CHEBI:26710"]
    ing3_NaCl -.-> CHEBI_26710
    ing4_Agar["Agar"]
    CultureMech_000720 --> ing4_Agar
    CHEBI_2509["CHEBI:2509"]
    ing4_Agar -.-> CHEBI_2509

Ingredients (5)

#	Ingredient	Concentration	Ontology
1	Yeast extract	4 G_PER_L
2	Malt extract	10 G_PER_L
3	Glucose C6H12O6 180.156 g/mol	4 G_PER_L	CHEBI:17234 glucose
4	NaCl Cl.Na 58.443 g/mol	100 G_PER_L	CHEBI:26710 sodium chloride
5	Agar	20 G_PER_L	CHEBI:2509 agar

Preparation

pH 7.3 - 7.5

Target organisms

No target organisms are recorded.

Provenance

Timestamp	Curator	Action	Notes
2026-01-26T17:53:13.884673Z	mediadive-import	Imported from MediaDive	Source: DSMZ, ID: 1261
2026-03-13T06:18:57.754144Z	culturemech-id-assigner-v1.0	Assigned CultureMech ID	Assigned stable identifier: CultureMech:000720
2026-03-17T06:29:53.000000Z	mediaingredientmech-enrichment-v1.0	Added MediaIngredientMech links	Linked ingredients to MediaIngredientMech identifiers
2026-03-17T19:45:09.073253+00:00	normalize_media_names_to_snake_case.py	NAME_NORMALIZED	Normalized name from "ISP 2 MEDIUM WITH 10% NACL" to "isp_2_medium_with_10_nacl" for consistent matching
2026-05-20T06:01:23.363197+00:00	migrate_ph_range.py	MIGRATED_PH_RANGE	ph_range '7.3-7.5' -> {'min': 7.3, 'max': 7.5}
2026-06-05T07:41:09.672672+00:00	mim-legacy-to-chebi-migration-v1.0	Refreshed legacy MediaIngredientMech links to CHEBI keying	Replaced 3 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme.
2026-10-07T13:32:40.086487+00:00	codex	CURATED_MEDIUM_CONCENTRATION_SOURCE_EVIDENCE	Exact medium/source membership, ingredient inventory/forms, physical-state conditions, literal snippets, and arithmetic checked. Stock boundaries, source-only rows, preparation context, and variants remain explicit follow-up tasks; secondary chemical mappings unchanged.