Overview
- External ontology
- mediadive.medium:1262 (source link unavailable) — CM + YE MEDIUM + 10% NACL
- Applications
-
- Microbial cultivation
- pH
- 7.4
- High-metal flag
- True
- Notes
- Source: DSMZ | Link: https://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium1262.pdf
Composition
flowchart LR
CultureMech_000721["cm_ye_medium_10_nacl"]
ing0_Casamino_acids["Casamino acids"]
CultureMech_000721 --> ing0_Casamino_acids
ing1_Yeast_extract["Yeast extract"]
CultureMech_000721 --> ing1_Yeast_extract
ing2_MgSO4_x_7_H2O["MgSO4 x 7 H2O"]
CultureMech_000721 --> ing2_MgSO4_x_7_H2O
CHEBI_31795["CHEBI:31795"]
ing2_MgSO4_x_7_H2O -.-> CHEBI_31795
ing3_Na3_citrate["Na3-citrate"]
CultureMech_000721 --> ing3_Na3_citrate
CHEBI_53258["CHEBI:53258"]
ing3_Na3_citrate -.-> CHEBI_53258
ing4_KCl["KCl"]
CultureMech_000721 --> ing4_KCl
CHEBI_32588["CHEBI:32588"]
ing4_KCl -.-> CHEBI_32588
ing5_NaCl["NaCl"]
CultureMech_000721 --> ing5_NaCl
CHEBI_26710["CHEBI:26710"]
ing5_NaCl -.-> CHEBI_26710
ing6_Fe_SO4_x_7_H2O["Fe SO4 x 7 H2O"]
CultureMech_000721 --> ing6_Fe_SO4_x_7_H2O
CHEBI_75836["CHEBI:75836"]
ing6_Fe_SO4_x_7_H2O -.-> CHEBI_75836
ing7_Agar["Agar"]
CultureMech_000721 --> ing7_Agar
CHEBI_2509["CHEBI:2509"]
ing7_Agar -.-> CHEBI_2509
Ingredients (8)
| # | Ingredient | Concentration | Ontology | Notes |
|---|---|---|---|---|
| 1 | Casamino acids | 7.5 G_PER_L | ||
| 2 | Yeast extract | 10 G_PER_L | ||
| 3 |
MgSO4 x 7 H2O
7H2O.Mg.O4S 246.473 g/mol
|
20 G_PER_L |
CHEBI:31795
magnesium sulfate heptahydrate
|
|
| 4 |
Na3-citrate
C6H5O7.3Na 258.069 g/mol
|
3 G_PER_L |
CHEBI:53258
sodium citrate
|
|
| 5 |
KCl
Cl.K 74.551 g/mol
|
2 G_PER_L |
CHEBI:32588
potassium chloride
|
|
| 6 |
NaCl
Cl.Na 58.443 g/mol
|
300.0 G_PER_L |
CHEBI:26710
sodium chloride
|
[Merged 2 duplicates: 200.0, 100.0] |
| 7 |
Fe SO4 x 7 H2O
Fe.7H2O.O4S 278.013 g/mol
|
1 G_PER_L |
CHEBI:75836
iron(2+) sulfate heptahydrate
|
|
| 8 | Agar | 15 G_PER_L |
CHEBI:2509
agar
|
Preparation
- pH 7.4 (NaOH)
- Prepare 4.98% FeSO4 x 7 H2O (= 10 g Fe in 1l 0.001 M HCl); add 1 ml per liter medium.
Target organisms
No target organisms are recorded.
Provenance
| Timestamp | Curator | Action | Notes |
|---|---|---|---|
| 2026-01-26T17:53:13.886130Z | mediadive-import | Imported from MediaDive | Source: DSMZ, ID: 1262 |
| 2026-01-28T19:48:22.851943Z | chebi-enrichment | Added CHEBI ontology terms to ingredients | Enriched using MicrobeMediaParam and MediaDive chemical mappings |
| 2026-03-13T03:09:07.777881Z | data-quality-cleanup-v1.0 | Data quality fixes | Merged 1 duplicate ingredient(s) |
| 2026-03-13T06:18:57.758922Z | culturemech-id-assigner-v1.0 | Assigned CultureMech ID | Assigned stable identifier: CultureMech:000721 |
| 2026-03-17T06:30:14.000000Z | mediaingredientmech-enrichment-v1.0 | Added MediaIngredientMech links | Linked ingredients to MediaIngredientMech identifiers |
| 2026-03-17T19:45:09.078353+00:00 | normalize_media_names_to_snake_case.py | NAME_NORMALIZED | Normalized name from "CM + YE MEDIUM + 10% NACL" to "cm_ye_medium_10_nacl" for consistent matching |
| 2026-06-05T07:40:54.545297+00:00 | mim-legacy-to-chebi-migration-v1.0 | Refreshed legacy MediaIngredientMech links to CHEBI keying | Replaced 7 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme. |
| 2026-06-08T23:19:36.409451+00:00 | chebi-regrounding-v1.0 | Corrected CHEBI ingredient grounding (audit G21/G22) | Re-grounded 2 term reference(s) to the correct CHEBI (see reports/chebi_grounding_audit.md). Label-conditional fixes for pyridoxine/pyridoxamine, glycerol, MnSO4, Ca(NO3)2, selenate; casamino acids de-grounded (mixture, no single CHEBI). |