medium_gy_vl55

CultureMech:000726 bacterial COMPLEX SOLID_AGAR
Original name: MEDIUM GY VL55

Overview

External ontology
mediadive.medium:1266a (source link unavailable) — MEDIUM GY VL55
Applications
  • Microbial cultivation
pH
5.5
Notes
Source: DSMZ | Link: https://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium1266a.pdf

Composition

flowchart LR
    CultureMech_000726["medium_gy_vl55"]
    ing0_MES["MES"]
    CultureMech_000726 --> ing0_MES
    CHEBI_39010["CHEBI:39010"]
    ing0_MES -.-> CHEBI_39010
    ing1_Glucose["Glucose"]
    CultureMech_000726 --> ing1_Glucose
    CHEBI_17234["CHEBI:17234"]
    ing1_Glucose -.-> CHEBI_17234
    ing2_Yeast_extract["Yeast extract"]
    CultureMech_000726 --> ing2_Yeast_extract
    ing3_Agar["Agar"]
    CultureMech_000726 --> ing3_Agar
    CHEBI_2509["CHEBI:2509"]
    ing3_Agar -.-> CHEBI_2509
    ing4_MgSO4_x_7_H2O["MgSO4 x 7 H2O"]
    CultureMech_000726 --> ing4_MgSO4_x_7_H2O
    CHEBI_31795["CHEBI:31795"]
    ing4_MgSO4_x_7_H2O -.-> CHEBI_31795
    ing5_CaCl2_x_2_H2O["CaCl2 x 2 H2O"]
    CultureMech_000726 --> ing5_CaCl2_x_2_H2O
    CHEBI_86158["CHEBI:86158"]
    ing5_CaCl2_x_2_H2O -.-> CHEBI_86158
    ing6__NH4_2HPO4["(NH4)2HPO4"]
    CultureMech_000726 --> ing6__NH4_2HPO4
    CHEBI_63051["CHEBI:63051"]
    ing6__NH4_2HPO4 -.-> CHEBI_63051
    ing7_NaOH["NaOH"]
    CultureMech_000726 --> ing7_NaOH
    CHEBI_32145["CHEBI:32145"]
    ing7_NaOH -.-> CHEBI_32145
    ing8_KOH["KOH"]
    CultureMech_000726 --> ing8_KOH
    CHEBI_32035["CHEBI:32035"]
    ing8_KOH -.-> CHEBI_32035
    ing9_Na2SeO3_x_5_H2O["Na2SeO3 x 5 H2O"]
    CultureMech_000726 --> ing9_Na2SeO3_x_5_H2O
    CHEBI_131361["CHEBI:131361"]
    ing9_Na2SeO3_x_5_H2O -.-> CHEBI_131361
    ing10_Na2WO4_x_2_H2O["Na2WO4 x 2 H2O"]
    CultureMech_000726 --> ing10_Na2WO4_x_2_H2O
    CHEBI_63939["CHEBI:63939"]
    ing10_Na2WO4_x_2_H2O -.-> CHEBI_63939
    ing11_HCl["HCl"]
    CultureMech_000726 --> ing11_HCl
    CHEBI_17883["CHEBI:17883"]
    ing11_HCl -.-> CHEBI_17883
    ing12_FeCl2_x_4_H2O["FeCl2 x 4 H2O"]
    CultureMech_000726 --> ing12_FeCl2_x_4_H2O
    CHEBI_86249["CHEBI:86249"]
    ing12_FeCl2_x_4_H2O -.-> CHEBI_86249
    ing13_ZnCl2["ZnCl2"]
    CultureMech_000726 --> ing13_ZnCl2
    CHEBI_49976["CHEBI:49976"]
    ing13_ZnCl2 -.-> CHEBI_49976
    ing14_MnCl2_x_4_H2O["MnCl2 x 4 H2O"]
    CultureMech_000726 --> ing14_MnCl2_x_4_H2O
    CHEBI_86368["CHEBI:86368"]
    ing14_MnCl2_x_4_H2O -.-> CHEBI_86368
    ing15_H3BO3["H3BO3"]
    CultureMech_000726 --> ing15_H3BO3
    CHEBI_33118["CHEBI:33118"]
    ing15_H3BO3 -.-> CHEBI_33118
    ing16_CoCl2_x_6_H2O["CoCl2 x 6 H2O"]
    CultureMech_000726 --> ing16_CoCl2_x_6_H2O
    CHEBI_53503["CHEBI:53503"]
    ing16_CoCl2_x_6_H2O -.-> CHEBI_53503
    ing17_CuCl2_x_2_H2O["CuCl2 x 2 H2O"]
    CultureMech_000726 --> ing17_CuCl2_x_2_H2O
    CHEBI_86318["CHEBI:86318"]
    ing17_CuCl2_x_2_H2O -.-> CHEBI_86318
    ing18_NiCl2_x_6_H2O["NiCl2 x 6 H2O"]
    CultureMech_000726 --> ing18_NiCl2_x_6_H2O
    CHEBI_34887["CHEBI:34887"]
    ing18_NiCl2_x_6_H2O -.-> CHEBI_34887
    ing19_Na2MoO4_x_2_H2O["Na2MoO4 x 2 H2O"]
    CultureMech_000726 --> ing19_Na2MoO4_x_2_H2O
    CHEBI_75213["CHEBI:75213"]
    ing19_Na2MoO4_x_2_H2O -.-> CHEBI_75213

Ingredients (20)

# Ingredient Concentration Ontology Notes
1 MES 3.96341 G_PER_L CHEBI:39010
MES
2 Glucose
C6H12O6 180.156 g/mol
2 G_PER_L CHEBI:17234
glucose
3 Yeast extract 1 G_PER_L
4 Agar 40 G_PER_L CHEBI:2509
agar
 (for solid medium)
5 MgSO4 x 7 H2O
7H2O.Mg.O4S 246.473 g/mol
4.9 G_PER_L CHEBI:31795
magnesium sulfate heptahydrate
6 CaCl2 x 2 H2O
Ca.2Cl.2H2O 147.014 g/mol
4.4 G_PER_L CHEBI:86158
calcium chloride dihydrate
7 (NH4)2HPO4
2H4N.HO4P 132.056 g/mol
2.6 G_PER_L CHEBI:63051
diammonium hydrogen phosphate
8 NaOH
HO.Na 39.997 g/mol
8.5 G_PER_L CHEBI:32145
sodium hydroxide
 [Merged 2 duplicates: 8.0, 0.5]
9 KOH
HO.K 56.105 g/mol
5.6 G_PER_L CHEBI:32035
potassium hydroxide
10 Na2SeO3 x 5 H2O
5H2O.2Na.O3Se 263.012 g/mol
0.003 G_PER_L CHEBI:131361
disodium selenite pentahydrate
11 Na2WO4 x 2 H2O
2H2O.2Na.O4W 329.846 g/mol
0.004 G_PER_L CHEBI:63939
sodium tungstate dihydrate
12 HCl
HCl 36.461 g/mol
2.5 G_PER_L CHEBI:17883
hydrogen chloride
13 FeCl2 x 4 H2O
Cl2Fe.4H2O 198.811 g/mol
1.5 G_PER_L CHEBI:86249
iron dichloride tetrahydrate
14 ZnCl2
2Cl.Zn 136.296 g/mol
0.07 G_PER_L CHEBI:49976
zinc dichloride
15 MnCl2 x 4 H2O
Cl2Mn.4H2O 197.904 g/mol
0.1 G_PER_L CHEBI:86368
manganese(II) chloride tetrahydrate
16 H3BO3
H3BO3 61.833 g/mol
0.006 G_PER_L CHEBI:33118
boric acid
17 CoCl2 x 6 H2O
2Cl.Co.6H2O 237.929 g/mol
0.19 G_PER_L CHEBI:53503
cobalt chloride hexahydrate
18 CuCl2 x 2 H2O
Cl2Cu.2H2O 170.482 g/mol
0.002 G_PER_L CHEBI:86318
copper(II) chloride dihydrate
19 NiCl2 x 6 H2O
Cl2Ni 129.599 g/mol
0.024 G_PER_L CHEBI:34887
nickel dichloride
20 Na2MoO4 x 2 H2O
2H2O.MoO4.2Na 241.946 g/mol
0.036 G_PER_L CHEBI:75213
sodium molybdate dihydrate

Preparation

  1. After autoclaving, cool to 50-60°C and combine the solution by pouring solution 2 to solution 1.
  2. Adjust to pH 5.5 with NaOH/KOH solution (see below).
  3. First dissolve FeCl2 in the HCl, then dilute in water, add and dissolve the other salts. Finally make up to 1000.00 ml.

Target organisms

No target organisms are recorded.

Provenance

TimestampCuratorActionNotes
2026-01-26T17:53:13.903630Z mediadive-import Imported from MediaDive Source: DSMZ, ID: 1266a
2026-03-13T03:09:13.109445Z data-quality-cleanup-v1.0 Data quality fixes Merged 1 duplicate ingredient(s)
2026-03-13T06:18:57.811689Z culturemech-id-assigner-v1.0 Assigned CultureMech ID Assigned stable identifier: CultureMech:000726
2026-03-17T06:30:20.000000Z mediaingredientmech-enrichment-v1.0 Added MediaIngredientMech links Linked ingredients to MediaIngredientMech identifiers
2026-03-17T19:45:09.136632+00:00 normalize_media_names_to_snake_case.py NAME_NORMALIZED Normalized name from "MEDIUM GY VL55" to "medium_gy_vl55" for consistent matching
2026-06-05T07:41:30.177505+00:00 mim-legacy-to-chebi-migration-v1.0 Refreshed legacy MediaIngredientMech links to CHEBI keying Replaced 18 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme.
2026-06-10T07:37:35.426215+00:00 primary-term-regrounding-v1.0 Re-grounded wrong primary term id (OAK-verified) 1 primary term(s); ingredient name resolved by OAK sqlite:obo:chebi.
2026-06-10T07:47:06.470031+00:00 primary-term-label-canonicalization-v1.0 Canonicalized primary term label to OBO canonical (OAK) 2 primary term(s); ingredient name resolved by OAK sqlite:obo:chebi.