ferriphaselus_es_medium

Overview

External ontology

mediadive.medium:1267 (source link unavailable) — FERRIPHASELUS (ES) MEDIUM

Applications

• Microbial cultivation

pH range

{'min': 6.1, 'max': 6.4}

Notes

Source: DSMZ | Link: https://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium1267.pdf

Composition

flowchart LR
    CultureMech_000727["ferriphaselus_es_medium"]
    ing0_NH4Cl["NH4Cl"]
    CultureMech_000727 --> ing0_NH4Cl
    CHEBI_31206["CHEBI:31206"]
    ing0_NH4Cl -.-> CHEBI_31206
    ing1_MgSO4_x_7_H2O["MgSO4 x 7 H2O"]
    CultureMech_000727 --> ing1_MgSO4_x_7_H2O
    CHEBI_31795["CHEBI:31795"]
    ing1_MgSO4_x_7_H2O -.-> CHEBI_31795
    ing2_CaCl2_x_2_H2O["CaCl2 x 2 H2O"]
    CultureMech_000727 --> ing2_CaCl2_x_2_H2O
    CHEBI_86158["CHEBI:86158"]
    ing2_CaCl2_x_2_H2O -.-> CHEBI_86158
    ing3_K2HPO4["K2HPO4"]
    CultureMech_000727 --> ing3_K2HPO4
    CHEBI_131527["CHEBI:131527"]
    ing3_K2HPO4 -.-> CHEBI_131527
    ing4_MES["MES"]
    CultureMech_000727 --> ing4_MES
    CHEBI_39010["CHEBI:39010"]
    ing4_MES -.-> CHEBI_39010
    ing5_NaHCO3["NaHCO3"]
    CultureMech_000727 --> ing5_NaHCO3
    CHEBI_32139["CHEBI:32139"]
    ing5_NaHCO3 -.-> CHEBI_32139
    ing6_Agarose["Agarose"]
    CultureMech_000727 --> ing6_Agarose
    CHEBI_2511["CHEBI:2511"]
    ing6_Agarose -.-> CHEBI_2511
    ing7_Nitrilotriacetic_acid["Nitrilotriacetic acid"]
    CultureMech_000727 --> ing7_Nitrilotriacetic_acid
    CHEBI_44557["CHEBI:44557"]
    ing7_Nitrilotriacetic_acid -.-> CHEBI_44557
    ing8_MnSO4_x_H2O["MnSO4 x H2O"]
    CultureMech_000727 --> ing8_MnSO4_x_H2O
    CHEBI_86364["CHEBI:86364"]
    ing8_MnSO4_x_H2O -.-> CHEBI_86364
    ing9_NaCl["NaCl"]
    CultureMech_000727 --> ing9_NaCl
    CHEBI_26710["CHEBI:26710"]
    ing9_NaCl -.-> CHEBI_26710
    ing10_FeSO4_x_7_H2O["FeSO4 x 7 H2O"]
    CultureMech_000727 --> ing10_FeSO4_x_7_H2O
    CHEBI_75836["CHEBI:75836"]
    ing10_FeSO4_x_7_H2O -.-> CHEBI_75836
    ing11_CoSO4_x_7_H2O["CoSO4 x 7 H2O"]
    CultureMech_000727 --> ing11_CoSO4_x_7_H2O
    CHEBI_91244["CHEBI:91244"]
    ing11_CoSO4_x_7_H2O -.-> CHEBI_91244
    ing12_ZnSO4_x_7_H2O["ZnSO4 x 7 H2O"]
    CultureMech_000727 --> ing12_ZnSO4_x_7_H2O
    CHEBI_32312["CHEBI:32312"]
    ing12_ZnSO4_x_7_H2O -.-> CHEBI_32312
    ing13_CuSO4_x_5_H2O["CuSO4 x 5 H2O"]
    CultureMech_000727 --> ing13_CuSO4_x_5_H2O
    CHEBI_31440["CHEBI:31440"]
    ing13_CuSO4_x_5_H2O -.-> CHEBI_31440
    ing14_AlK_SO4_2_x_12_H2O["AlK(SO4)2 x 12 H2O"]
    CultureMech_000727 --> ing14_AlK_SO4_2_x_12_H2O
    CHEBI_86465["CHEBI:86465"]
    ing14_AlK_SO4_2_x_12_H2O -.-> CHEBI_86465
    ing15_H3BO3["H3BO3"]
    CultureMech_000727 --> ing15_H3BO3
    CHEBI_33118["CHEBI:33118"]
    ing15_H3BO3 -.-> CHEBI_33118
    ing16_Na2MoO4_x_2_H2O["Na2MoO4 x 2 H2O"]
    CultureMech_000727 --> ing16_Na2MoO4_x_2_H2O
    CHEBI_75213["CHEBI:75213"]
    ing16_Na2MoO4_x_2_H2O -.-> CHEBI_75213
    ing17_NiCl2_x_6_H2O["NiCl2 x 6 H2O"]
    CultureMech_000727 --> ing17_NiCl2_x_6_H2O
    CHEBI_34887["CHEBI:34887"]
    ing17_NiCl2_x_6_H2O -.-> CHEBI_34887
    ing18_Na2SeO3_x_5_H2O["Na2SeO3 x 5 H2O"]
    CultureMech_000727 --> ing18_Na2SeO3_x_5_H2O
    CHEBI_131361["CHEBI:131361"]
    ing18_Na2SeO3_x_5_H2O -.-> CHEBI_131361
    ing19_Na2WO4_x_2_H2O["Na2WO4 x 2 H2O"]
    CultureMech_000727 --> ing19_Na2WO4_x_2_H2O
    CHEBI_63939["CHEBI:63939"]
    ing19_Na2WO4_x_2_H2O -.-> CHEBI_63939
    ing20_Biotin["Biotin"]
    CultureMech_000727 --> ing20_Biotin
    CHEBI_15956["CHEBI:15956"]
    ing20_Biotin -.-> CHEBI_15956
    ing21_Folic_acid["Folic acid"]
    CultureMech_000727 --> ing21_Folic_acid
    CHEBI_27470["CHEBI:27470"]
    ing21_Folic_acid -.-> CHEBI_27470
    ing22_Pyridoxine_hydrochloride["Pyridoxine hydrochloride"]
    CultureMech_000727 --> ing22_Pyridoxine_hydrochloride
    CHEBI_30961["CHEBI:30961"]
    ing22_Pyridoxine_hydrochloride -.-> CHEBI_30961
    ing23_Thiamine_HCl["Thiamine HCl"]
    CultureMech_000727 --> ing23_Thiamine_HCl
    CHEBI_49105["CHEBI:49105"]
    ing23_Thiamine_HCl -.-> CHEBI_49105
    ing24_Riboflavin["Riboflavin"]
    CultureMech_000727 --> ing24_Riboflavin
    CHEBI_17015["CHEBI:17015"]
    ing24_Riboflavin -.-> CHEBI_17015
    ing25_Nicotinic_acid["Nicotinic acid"]
    CultureMech_000727 --> ing25_Nicotinic_acid
    CHEBI_15940["CHEBI:15940"]
    ing25_Nicotinic_acid -.-> CHEBI_15940
    ing26_Calcium_D_____pantothenate["Calcium D-(+)-pantothenate"]
    CultureMech_000727 --> ing26_Calcium_D_____pantothenate
    CHEBI_31345["CHEBI:31345"]
    ing26_Calcium_D_____pantothenate -.-> CHEBI_31345
    ing27_Vitamin_B12["Vitamin B12"]
    CultureMech_000727 --> ing27_Vitamin_B12
    CHEBI_176843["CHEBI:176843"]
    ing27_Vitamin_B12 -.-> CHEBI_176843
    ing28_p_Aminobenzoic_acid["p-Aminobenzoic acid"]
    CultureMech_000727 --> ing28_p_Aminobenzoic_acid
    CHEBI_30753["CHEBI:30753"]
    ing28_p_Aminobenzoic_acid -.-> CHEBI_30753
    ing29__DL__alpha_Lipoic_acid["(DL)-alpha-Lipoic acid"]
    CultureMech_000727 --> ing29__DL__alpha_Lipoic_acid
    CHEBI_16494["CHEBI:16494"]
    ing29__DL__alpha_Lipoic_acid -.-> CHEBI_16494
    CultureMech_000727 --> more["...1 more"]

Ingredients (31)

Preparation

1. Preparation of the bottom layer: Mix 1 volume of solution A (except MES buffer, NaHCO3, and agarose) with 1 volume of solution C and add 1% (w/v) agarose type 1, low EEO. After autoclaving, aseptically fill 1 ml of the suspension in sterile Hungate-type tubes (15 ml total volume). The bottom layer solidifies in approx. 30 min.
2. Preparation of the top layer: Add MES buffer, bicarbonate and low melt agarose to solution A and autoclave. Let the sterile solution cool to 40°C and add 10.00 ml/l of solution B. Sparge solution with sterile CO2 gas until a pH of 6.1 - 6.4 is reached. Then, aseptically pipette aliquots of 5.0 ml over the bottom layer of each tube under 100% N2 gas atmosphere and let medium equilibrate for at least three hours, but not longer than 12 hours.
3. Inoculation: Inoculate the semisolid top layer under a stream of 80% N2 and 20% CO2 gas mixture using a sterile Pasteur pipette that is inserted just above the FeS layer; the pipette tip is drawn upward as the inoculum is dispensed. After inoculation close the tube and add 1.00 ml of sterile air.
4. Note: Some hints on the inoculation of Hungate-tubes under anoxic conditions can be found in the Video tutorial on the „Proper Handling of the Double-Vial Glass Ampoules (anaerobe)" (www.dsmz.de/shop-1/faqs/video-tutorials).
5. Sterilize by filtration and store at 4°C in the dark.
6. First dissolve nitrilotriacetic acid and adjust pH to 6.5 with KOH, then add minerals. Adjust final to pH 7.0 with KOH.
7. Heat distilled water to 50°C in a 250 ml beaker with a stir bar present. While rapidly stirring the water, add the ferrous sulfate followed immediately by the sodium sulfide. The formed black FeS sludge is decanted into a glass bottle that can be stoppered. The FeS is allowed to settle for several hours and then the overlying water is decanted and replaced. This procedure is repeated at least five times to wash the FeS. After washing, the pH of the FeS solution should be close to neutrality. The FeS suspension can be kept in closed bottles or tubes under a nitrogen atmosphere for at least three months.

Target organisms

No target organisms are recorded.

Provenance