georgfuchsia_medium

CultureMech:000736 bacterial DEFINED LIQUID
Original name: GEORGFUCHSIA MEDIUM

Overview

External ontology
mediadive.medium:1275 (source link unavailable) — GEORGFUCHSIA MEDIUM
Applications
  • Microbial cultivation
pH
7.3
Notes
Source: DSMZ | Link: https://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium1275.pdf

Composition

flowchart LR
    CultureMech_000736["georgfuchsia_medium"]
    ing0_KH2PO4["KH2PO4"]
    CultureMech_000736 --> ing0_KH2PO4
    CHEBI_63036["CHEBI:63036"]
    ing0_KH2PO4 -.-> CHEBI_63036
    ing1_Na2HPO4["Na2HPO4"]
    CultureMech_000736 --> ing1_Na2HPO4
    CHEBI_34683["CHEBI:34683"]
    ing1_Na2HPO4 -.-> CHEBI_34683
    ing2_Resazurin["Resazurin"]
    CultureMech_000736 --> ing2_Resazurin
    CHEBI_8806["CHEBI:8806"]
    ing2_Resazurin -.-> CHEBI_8806
    ing3_CaCl2["CaCl2"]
    CultureMech_000736 --> ing3_CaCl2
    CHEBI_3312["CHEBI:3312"]
    ing3_CaCl2 -.-> CHEBI_3312
    ing4_MgCl2["MgCl2"]
    CultureMech_000736 --> ing4_MgCl2
    CHEBI_6636["CHEBI:6636"]
    ing4_MgCl2 -.-> CHEBI_6636
    ing5_NH4HCO3["NH4HCO3"]
    CultureMech_000736 --> ing5_NH4HCO3
    CHEBI_184335["CHEBI:184335"]
    ing5_NH4HCO3 -.-> CHEBI_184335
    ing6_NaHCO3["NaHCO3"]
    CultureMech_000736 --> ing6_NaHCO3
    CHEBI_32139["CHEBI:32139"]
    ing6_NaHCO3 -.-> CHEBI_32139
    ing7_L_Cysteine_HCl_x_H2O["L-Cysteine HCl x H2O"]
    CultureMech_000736 --> ing7_L_Cysteine_HCl_x_H2O
    CHEBI_91248["CHEBI:91248"]
    ing7_L_Cysteine_HCl_x_H2O -.-> CHEBI_91248
    ing8_NaNO3["NaNO3"]
    CultureMech_000736 --> ing8_NaNO3
    CHEBI_63005["CHEBI:63005"]
    ing8_NaNO3 -.-> CHEBI_63005
    ing9_Toluene["Toluene"]
    CultureMech_000736 --> ing9_Toluene
    CHEBI_17578["CHEBI:17578"]
    ing9_Toluene -.-> CHEBI_17578
    ing10_HCl["HCl"]
    CultureMech_000736 --> ing10_HCl
    CHEBI_17883["CHEBI:17883"]
    ing10_HCl -.-> CHEBI_17883
    ing11_FeCl2_x_4_H2O["FeCl2 x 4 H2O"]
    CultureMech_000736 --> ing11_FeCl2_x_4_H2O
    CHEBI_86249["CHEBI:86249"]
    ing11_FeCl2_x_4_H2O -.-> CHEBI_86249
    ing12_ZnCl2["ZnCl2"]
    CultureMech_000736 --> ing12_ZnCl2
    CHEBI_49976["CHEBI:49976"]
    ing12_ZnCl2 -.-> CHEBI_49976
    ing13_MnCl2_x_4_H2O["MnCl2 x 4 H2O"]
    CultureMech_000736 --> ing13_MnCl2_x_4_H2O
    CHEBI_86368["CHEBI:86368"]
    ing13_MnCl2_x_4_H2O -.-> CHEBI_86368
    ing14_H3BO3["H3BO3"]
    CultureMech_000736 --> ing14_H3BO3
    CHEBI_33118["CHEBI:33118"]
    ing14_H3BO3 -.-> CHEBI_33118
    ing15_CoCl2_x_6_H2O["CoCl2 x 6 H2O"]
    CultureMech_000736 --> ing15_CoCl2_x_6_H2O
    CHEBI_53503["CHEBI:53503"]
    ing15_CoCl2_x_6_H2O -.-> CHEBI_53503
    ing16_CuCl2_x_2_H2O["CuCl2 x 2 H2O"]
    CultureMech_000736 --> ing16_CuCl2_x_2_H2O
    CHEBI_86318["CHEBI:86318"]
    ing16_CuCl2_x_2_H2O -.-> CHEBI_86318
    ing17_NiCl2_x_6_H2O["NiCl2 x 6 H2O"]
    CultureMech_000736 --> ing17_NiCl2_x_6_H2O
    CHEBI_34887["CHEBI:34887"]
    ing17_NiCl2_x_6_H2O -.-> CHEBI_34887
    ing18_Na2MoO4_x_2_H2O["Na2MoO4 x 2 H2O"]
    CultureMech_000736 --> ing18_Na2MoO4_x_2_H2O
    CHEBI_75213["CHEBI:75213"]
    ing18_Na2MoO4_x_2_H2O -.-> CHEBI_75213
    ing19_NaOH["NaOH"]
    CultureMech_000736 --> ing19_NaOH
    CHEBI_32145["CHEBI:32145"]
    ing19_NaOH -.-> CHEBI_32145
    ing20_Na2SeO3_x_5_H2O["Na2SeO3 x 5 H2O"]
    CultureMech_000736 --> ing20_Na2SeO3_x_5_H2O
    CHEBI_131361["CHEBI:131361"]
    ing20_Na2SeO3_x_5_H2O -.-> CHEBI_131361
    ing21_Na2WO4_x_2_H2O["Na2WO4 x 2 H2O"]
    CultureMech_000736 --> ing21_Na2WO4_x_2_H2O
    CHEBI_63939["CHEBI:63939"]
    ing21_Na2WO4_x_2_H2O -.-> CHEBI_63939
    ing22_Biotin["Biotin"]
    CultureMech_000736 --> ing22_Biotin
    CHEBI_15956["CHEBI:15956"]
    ing22_Biotin -.-> CHEBI_15956
    ing23_Folic_acid["Folic acid"]
    CultureMech_000736 --> ing23_Folic_acid
    CHEBI_27470["CHEBI:27470"]
    ing23_Folic_acid -.-> CHEBI_27470
    ing24_Pyridoxine_hydrochloride["Pyridoxine hydrochloride"]
    CultureMech_000736 --> ing24_Pyridoxine_hydrochloride
    CHEBI_30961["CHEBI:30961"]
    ing24_Pyridoxine_hydrochloride -.-> CHEBI_30961
    ing25_Thiamine_HCl_x_2_H2O["Thiamine-HCl x 2 H2O"]
    CultureMech_000736 --> ing25_Thiamine_HCl_x_2_H2O
    CHEBI_132751["CHEBI:132751"]
    ing25_Thiamine_HCl_x_2_H2O -.-> CHEBI_132751
    ing26_Riboflavin["Riboflavin"]
    CultureMech_000736 --> ing26_Riboflavin
    CHEBI_17015["CHEBI:17015"]
    ing26_Riboflavin -.-> CHEBI_17015
    ing27_Nicotinic_acid["Nicotinic acid"]
    CultureMech_000736 --> ing27_Nicotinic_acid
    CHEBI_15940["CHEBI:15940"]
    ing27_Nicotinic_acid -.-> CHEBI_15940
    ing28_D_Calcium_pantothenate["D-Calcium pantothenate"]
    CultureMech_000736 --> ing28_D_Calcium_pantothenate
    CHEBI_31345["CHEBI:31345"]
    ing28_D_Calcium_pantothenate -.-> CHEBI_31345
    ing29_Vitamin_B12["Vitamin B12"]
    CultureMech_000736 --> ing29_Vitamin_B12
    CHEBI_176843["CHEBI:176843"]
    ing29_Vitamin_B12 -.-> CHEBI_176843
    CultureMech_000736 --> more["...2 more"]

Ingredients (32)

# Ingredient Concentration Ontology Notes
1 KH2PO4
H2O4P.K 136.084 g/mol
0.718232 G_PER_L CHEBI:63036
potassium dihydrogen phosphate
2 Na2HPO4
HO4P.2Na 141.958 g/mol
0.220994 G_PER_L CHEBI:34683
disodium hydrogenphosphate
3 Resazurin
C12H7NO4 229.191 g/mol
0.000552486 G_PER_L CHEBI:8806
Resazurin
4 CaCl2
Ca.2Cl 110.984 g/mol
2.2 G_PER_L CHEBI:3312
calcium dichloride
5 MgCl2
2Cl.Mg 95.211 g/mol
2 G_PER_L CHEBI:6636
magnesium dichloride
6 NH4HCO3
CHO3.H4N 79.055 g/mol
8.8 G_PER_L CHEBI:184335
Ammonium bicarbonate
7 NaHCO3
CHO3.Na 84.006 g/mol
74.6 G_PER_L CHEBI:32139
sodium hydrogencarbonate
8 L-Cysteine HCl x H2O
C3H7NO2S.H2O.HCl 175.637 g/mol
0.6 G_PER_L CHEBI:91248
L-cysteine hydrochloride hydrate
9 NaNO3
NO3.Na 84.994 g/mol
34 G_PER_L CHEBI:63005
sodium nitrate
10 Toluene
C7H8 92.141 g/mol
58 G_PER_L CHEBI:17578
toluene
11 HCl
HCl 36.461 g/mol
2.5 G_PER_L CHEBI:17883
hydrogen chloride
12 FeCl2 x 4 H2O
Cl2Fe.4H2O 198.811 g/mol
1.5 G_PER_L CHEBI:86249
iron dichloride tetrahydrate
13 ZnCl2
2Cl.Zn 136.296 g/mol
0.07 G_PER_L CHEBI:49976
zinc dichloride
14 MnCl2 x 4 H2O
Cl2Mn.4H2O 197.904 g/mol
0.1 G_PER_L CHEBI:86368
manganese(II) chloride tetrahydrate
15 H3BO3
H3BO3 61.833 g/mol
0.006 G_PER_L CHEBI:33118
boric acid
16 CoCl2 x 6 H2O
2Cl.Co.6H2O 237.929 g/mol
0.19 G_PER_L CHEBI:53503
cobalt chloride hexahydrate
17 CuCl2 x 2 H2O
Cl2Cu.2H2O 170.482 g/mol
0.002 G_PER_L CHEBI:86318
copper(II) chloride dihydrate
18 NiCl2 x 6 H2O
Cl2Ni 129.599 g/mol
0.024 G_PER_L CHEBI:34887
nickel dichloride
19 Na2MoO4 x 2 H2O
2H2O.MoO4.2Na 241.946 g/mol
0.036 G_PER_L CHEBI:75213
sodium molybdate dihydrate
20 NaOH
HO.Na 39.997 g/mol
0.5 G_PER_L CHEBI:32145
sodium hydroxide
21 Na2SeO3 x 5 H2O
5H2O.2Na.O3Se 263.012 g/mol
0.003 G_PER_L CHEBI:131361
disodium selenite pentahydrate
22 Na2WO4 x 2 H2O
2H2O.2Na.O4W 329.846 g/mol
0.004 G_PER_L CHEBI:63939
sodium tungstate dihydrate
23 Biotin
C10H16N2O3S 244.316 g/mol
0.02 G_PER_L CHEBI:15956
biotin
24 Folic acid
C19H19N7O6 441.404 g/mol
0.02 G_PER_L CHEBI:27470
folic acid
25 Pyridoxine hydrochloride
C8H11NO3.HCl 205.641 g/mol
0.1 G_PER_L CHEBI:30961
pyridoxine hydrochloride
26 Thiamine-HCl x 2 H2O
C12H18N4OS.2Cl.2H2O 373.306 g/mol
0.05 G_PER_L CHEBI:132751
thiamine hydrochloride dihydrate
27 Riboflavin
C17H20N4O6 376.369 g/mol
0.05 G_PER_L CHEBI:17015
riboflavin
28 Nicotinic acid
C6H5NO2 123.111 g/mol
0.05 G_PER_L CHEBI:15940
nicotinic acid
29 D-Calcium pantothenate
2C9H16NO5.Ca 476.536 g/mol
0.05 G_PER_L CHEBI:31345
Calcium pantothenate
30 Vitamin B12 0.001 G_PER_L CHEBI:176843
vitamin B12
31 p-Aminobenzoic acid
C7H7NO2 137.138 g/mol
0.05 G_PER_L CHEBI:30753
4-aminobenzoic acid
32 Lipoic acid
C8H14O2S2 206.332 g/mol
0.05 G_PER_L CHEBI:16494
lipoic acid

Preparation

  1. Solutions are prepared anaerobically under N2:CO2. Solutions A, B, C and D are autoclaved. After cooling, the solutions are combined under N2 gas. To 10 ml solution A, add 0.5 ml solution B, 0.5 ml solution C, 0.25 ml solution D and 0.4 ml solution E.
  2. pH should be 7.3 without further adjustment. Add new toluene solution to the cultures about every 7 days.
  3. Add 1 ml vitamin solution (see below) after autoclaving and cooling.
  4. Sterile distilled water gassed with dinitrogen, 100.00 ml. Add 58 µl toluene.
  5. First dissolve FeCl2 in the HCl, then dilute in water, add and dissolve the other salts. Finally make up to 1000.00 ml.

Target organisms

No target organisms are recorded.

Provenance

TimestampCuratorActionNotes
2026-01-26T17:53:13.952403Z mediadive-import Imported from MediaDive Source: DSMZ, ID: 1275
2026-01-28T19:48:28.159153Z chebi-enrichment Added CHEBI ontology terms to ingredients Enriched using MicrobeMediaParam and MediaDive chemical mappings
2026-03-13T06:18:57.927174Z culturemech-id-assigner-v1.0 Assigned CultureMech ID Assigned stable identifier: CultureMech:000736
2026-03-17T06:30:25.000000Z mediaingredientmech-enrichment-v1.0 Added MediaIngredientMech links Linked ingredients to MediaIngredientMech identifiers
2026-03-17T19:45:09.263796+00:00 normalize_media_names_to_snake_case.py NAME_NORMALIZED Normalized name from "GEORGFUCHSIA MEDIUM" to "georgfuchsia_medium" for consistent matching
2026-06-05T07:41:06.263973+00:00 mim-legacy-to-chebi-migration-v1.0 Refreshed legacy MediaIngredientMech links to CHEBI keying Replaced 31 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme.
2026-06-10T07:46:59.067651+00:00 primary-term-label-canonicalization-v1.0 Canonicalized primary term label to OBO canonical (OAK) 2 primary term(s); ingredient name resolved by OAK sqlite:obo:chebi.