caldimicrobium_thiodismutans_medium

CultureMech:000740 bacterial DEFINED LIQUID
Original name: CALDIMICROBIUM THIODISMUTANS MEDIUM

Overview

External ontology
mediadive.medium:1277b (source link unavailable) — CALDIMICROBIUM THIODISMUTANS MEDIUM
Applications
  • Microbial cultivation
pH range
{'min': 7.5, 'max': 8.0}
Notes
Source: DSMZ | Link: https://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium1277b.pdf

Composition

flowchart LR
    CultureMech_000740["caldimicrobium_thiodismutans_medium"]
    ing0_MgCl2_x_6_H2O["MgCl2 x 6 H2O"]
    CultureMech_000740 --> ing0_MgCl2_x_6_H2O
    CHEBI_86345["CHEBI:86345"]
    ing0_MgCl2_x_6_H2O -.-> CHEBI_86345
    ing1_CaCl2_x_2_H2O["CaCl2 x 2 H2O"]
    CultureMech_000740 --> ing1_CaCl2_x_2_H2O
    CHEBI_86158["CHEBI:86158"]
    ing1_CaCl2_x_2_H2O -.-> CHEBI_86158
    ing2__NH4_2SO4["(NH4)2SO4"]
    CultureMech_000740 --> ing2__NH4_2SO4
    CHEBI_62946["CHEBI:62946"]
    ing2__NH4_2SO4 -.-> CHEBI_62946
    ing3_KH2PO4["KH2PO4"]
    CultureMech_000740 --> ing3_KH2PO4
    CHEBI_63036["CHEBI:63036"]
    ing3_KH2PO4 -.-> CHEBI_63036
    ing4_KCl["KCl"]
    CultureMech_000740 --> ing4_KCl
    CHEBI_32588["CHEBI:32588"]
    ing4_KCl -.-> CHEBI_32588
    ing5_Na2CO3["Na2CO3"]
    CultureMech_000740 --> ing5_Na2CO3
    CHEBI_29377["CHEBI:29377"]
    ing5_Na2CO3 -.-> CHEBI_29377
    ing6_Na2S2O5["Na2S2O5"]
    CultureMech_000740 --> ing6_Na2S2O5
    CHEBI_114786["CHEBI:114786"]
    ing6_Na2S2O5 -.-> CHEBI_114786
    ing7_HCl["HCl"]
    CultureMech_000740 --> ing7_HCl
    CHEBI_17883["CHEBI:17883"]
    ing7_HCl -.-> CHEBI_17883
    ing8_FeCl2_x_4_H2O["FeCl2 x 4 H2O"]
    CultureMech_000740 --> ing8_FeCl2_x_4_H2O
    CHEBI_86249["CHEBI:86249"]
    ing8_FeCl2_x_4_H2O -.-> CHEBI_86249
    ing9_ZnCl2["ZnCl2"]
    CultureMech_000740 --> ing9_ZnCl2
    CHEBI_49976["CHEBI:49976"]
    ing9_ZnCl2 -.-> CHEBI_49976
    ing10_MnCl2_x_4_H2O["MnCl2 x 4 H2O"]
    CultureMech_000740 --> ing10_MnCl2_x_4_H2O
    CHEBI_86368["CHEBI:86368"]
    ing10_MnCl2_x_4_H2O -.-> CHEBI_86368
    ing11_H3BO3["H3BO3"]
    CultureMech_000740 --> ing11_H3BO3
    CHEBI_33118["CHEBI:33118"]
    ing11_H3BO3 -.-> CHEBI_33118
    ing12_CoCl2_x_6_H2O["CoCl2 x 6 H2O"]
    CultureMech_000740 --> ing12_CoCl2_x_6_H2O
    CHEBI_53503["CHEBI:53503"]
    ing12_CoCl2_x_6_H2O -.-> CHEBI_53503
    ing13_CuCl2_x_2_H2O["CuCl2 x 2 H2O"]
    CultureMech_000740 --> ing13_CuCl2_x_2_H2O
    CHEBI_86318["CHEBI:86318"]
    ing13_CuCl2_x_2_H2O -.-> CHEBI_86318
    ing14_NiCl2_x_6_H2O["NiCl2 x 6 H2O"]
    CultureMech_000740 --> ing14_NiCl2_x_6_H2O
    CHEBI_34887["CHEBI:34887"]
    ing14_NiCl2_x_6_H2O -.-> CHEBI_34887
    ing15_Na2MoO4_x_2_H2O["Na2MoO4 x 2 H2O"]
    CultureMech_000740 --> ing15_Na2MoO4_x_2_H2O
    CHEBI_75213["CHEBI:75213"]
    ing15_Na2MoO4_x_2_H2O -.-> CHEBI_75213
    ing16_NaOH["NaOH"]
    CultureMech_000740 --> ing16_NaOH
    CHEBI_32145["CHEBI:32145"]
    ing16_NaOH -.-> CHEBI_32145
    ing17_Na2SeO3_x_5_H2O["Na2SeO3 x 5 H2O"]
    CultureMech_000740 --> ing17_Na2SeO3_x_5_H2O
    CHEBI_131361["CHEBI:131361"]
    ing17_Na2SeO3_x_5_H2O -.-> CHEBI_131361
    ing18_Na2WO4_x_2_H2O["Na2WO4 x 2 H2O"]
    CultureMech_000740 --> ing18_Na2WO4_x_2_H2O
    CHEBI_63939["CHEBI:63939"]
    ing18_Na2WO4_x_2_H2O -.-> CHEBI_63939
    ing19_FeCl3_x_6_H2O["FeCl3 x 6 H2O"]
    CultureMech_000740 --> ing19_FeCl3_x_6_H2O
    CHEBI_86254["CHEBI:86254"]
    ing19_FeCl3_x_6_H2O -.-> CHEBI_86254
    ing20_Vitamin_B12["Vitamin B12"]
    CultureMech_000740 --> ing20_Vitamin_B12
    CHEBI_176843["CHEBI:176843"]
    ing20_Vitamin_B12 -.-> CHEBI_176843
    ing21_p_Aminobenzoic_acid["p-Aminobenzoic acid"]
    CultureMech_000740 --> ing21_p_Aminobenzoic_acid
    CHEBI_30753["CHEBI:30753"]
    ing21_p_Aminobenzoic_acid -.-> CHEBI_30753
    ing22_D_____biotin["D-(+)-biotin"]
    CultureMech_000740 --> ing22_D_____biotin
    CHEBI_15956["CHEBI:15956"]
    ing22_D_____biotin -.-> CHEBI_15956
    ing23_Nicotinic_acid["Nicotinic acid"]
    CultureMech_000740 --> ing23_Nicotinic_acid
    CHEBI_15940["CHEBI:15940"]
    ing23_Nicotinic_acid -.-> CHEBI_15940
    ing24_Calcium_pantothenate["Calcium pantothenate"]
    CultureMech_000740 --> ing24_Calcium_pantothenate
    CHEBI_31345["CHEBI:31345"]
    ing24_Calcium_pantothenate -.-> CHEBI_31345
    ing25_Pyridoxine_hydrochloride["Pyridoxine hydrochloride"]
    CultureMech_000740 --> ing25_Pyridoxine_hydrochloride
    CHEBI_30961["CHEBI:30961"]
    ing25_Pyridoxine_hydrochloride -.-> CHEBI_30961
    ing26_Thiamine_HCl_x_2_H2O["Thiamine-HCl x 2 H2O"]
    CultureMech_000740 --> ing26_Thiamine_HCl_x_2_H2O
    CHEBI_132751["CHEBI:132751"]
    ing26_Thiamine_HCl_x_2_H2O -.-> CHEBI_132751

Ingredients (27)

# Ingredient Concentration Ontology Notes
1 MgCl2 x 6 H2O
2Cl.6H2O.Mg 203.301 g/mol
0.2 G_PER_L CHEBI:86345
magnesium dichloride hexahydrate
2 CaCl2 x 2 H2O
Ca.2Cl.2H2O 147.014 g/mol
0.1 G_PER_L CHEBI:86158
calcium chloride dihydrate
3 (NH4)2SO4
2H4N.O4S 132.141 g/mol
0.1 G_PER_L CHEBI:62946
ammonium sulfate
4 KH2PO4
H2O4P.K 136.084 g/mol
0.1 G_PER_L CHEBI:63036
potassium dihydrogen phosphate
5 KCl
Cl.K 74.551 g/mol
0.1 G_PER_L CHEBI:32588
potassium chloride
6 Na2CO3
CO3.2Na 105.988 g/mol
2.5 G_PER_L CHEBI:29377
sodium carbonate
7 Na2S2O5
2Na.O5S2 190.109 g/mol
0.9 G_PER_L CHEBI:114786
sodium disulfite
8 HCl
HCl 36.461 g/mol
2.5 G_PER_L CHEBI:17883
hydrogen chloride
9 FeCl2 x 4 H2O
Cl2Fe.4H2O 198.811 g/mol
1.5 G_PER_L CHEBI:86249
iron dichloride tetrahydrate
10 ZnCl2
2Cl.Zn 136.296 g/mol
0.07 G_PER_L CHEBI:49976
zinc dichloride
11 MnCl2 x 4 H2O
Cl2Mn.4H2O 197.904 g/mol
0.1 G_PER_L CHEBI:86368
manganese(II) chloride tetrahydrate
12 H3BO3
H3BO3 61.833 g/mol
0.006 G_PER_L CHEBI:33118
boric acid
13 CoCl2 x 6 H2O
2Cl.Co.6H2O 237.929 g/mol
0.19 G_PER_L CHEBI:53503
cobalt chloride hexahydrate
14 CuCl2 x 2 H2O
Cl2Cu.2H2O 170.482 g/mol
0.002 G_PER_L CHEBI:86318
copper(II) chloride dihydrate
15 NiCl2 x 6 H2O
Cl2Ni 129.599 g/mol
0.024 G_PER_L CHEBI:34887
nickel dichloride
16 Na2MoO4 x 2 H2O
2H2O.MoO4.2Na 241.946 g/mol
0.036 G_PER_L CHEBI:75213
sodium molybdate dihydrate
17 NaOH
HO.Na 39.997 g/mol
20.5 G_PER_L CHEBI:32145
sodium hydroxide
 [Merged 2 duplicates: 0.5, 20.0]
18 Na2SeO3 x 5 H2O
5H2O.2Na.O3Se 263.012 g/mol
0.003 G_PER_L CHEBI:131361
disodium selenite pentahydrate
19 Na2WO4 x 2 H2O
2H2O.2Na.O4W 329.846 g/mol
0.004 G_PER_L CHEBI:63939
sodium tungstate dihydrate
20 FeCl3 x 6 H2O
Cl3Fe.6H2O 270.294 g/mol
320 G_PER_L CHEBI:86254
iron trichloride hexahydrate
21 Vitamin B12 0.1 G_PER_L CHEBI:176843
vitamin B12
22 p-Aminobenzoic acid
C7H7NO2 137.138 g/mol
0.08 G_PER_L CHEBI:30753
4-aminobenzoic acid
23 D-(+)-biotin
C10H16N2O3S 244.316 g/mol
0.02 G_PER_L CHEBI:15956
biotin
24 Nicotinic acid
C6H5NO2 123.111 g/mol
0.2 G_PER_L CHEBI:15940
nicotinic acid
25 Calcium pantothenate
2C9H16NO5.Ca 476.536 g/mol
0.1 G_PER_L CHEBI:31345
Calcium pantothenate
26 Pyridoxine hydrochloride
C8H11NO3.HCl 205.641 g/mol
0.3 G_PER_L CHEBI:30961
pyridoxine hydrochloride
27 Thiamine-HCl x 2 H2O
C12H18N4OS.2Cl.2H2O 373.306 g/mol
0.2 G_PER_L CHEBI:132751
thiamine hydrochloride dihydrate

Preparation

  1. Dissolve ingredients (except carbonate, pyrosulfite and vitamins), then sparge medium with 80% N2 and 20% CO2 gas mixture for 30 - 45 min to make it anoxic. Dispense medium under same gas atmosphere into Hungate-type tubes or serum vials and autoclave. Add pyrosulfite from a 10% (w/v) stock solution adjusted to pH 7.5-8.0 with NaOH, made anoxic with 100% N2 gas and sterilized by filtration. Vitamins are added from a stock solution prepared under 100% N2 gas atmosphere and sterilized by filtration and carbonate is added from a sterile stock solution prepared under 80% N2 and 20% CO2 gas atmosphere. Adjust pH of complete medium to 7.5 - 8.0. Incubate without shaking.
  2. First dissolve FeCl2 in the HCl, then dilute in water, add and dissolve the other salts. Finally make up to 1000.00 ml.
  3. Slowly titrate 320 ml of a FeCl3 x 6 H2O stock solution (60.00 g/l) with 10% (w/v) NaOH to pH 8.0-8.5 under agitation (use magnetic stirrer). Total amount of added NaOH approx. 80 - 100 ml. The precipitated Fe(OH)3 should be stored at room temperature overnight with surface covered with water. Thereafter, centrifuge at 2000 rpm for 5 min and discard the supernatant. Wash several times with distilled water. Resuspend the pellet in medium as described above. For storage autoclave under 100% N2 atmosphere.

Target organisms

No target organisms are recorded.

Provenance

TimestampCuratorActionNotes
2026-01-26T17:53:13.968904Z mediadive-import Imported from MediaDive Source: DSMZ, ID: 1277b
2026-03-13T03:08:38.575438Z data-quality-cleanup-v1.0 Data quality fixes Merged 1 duplicate ingredient(s)
2026-03-13T06:18:57.976545Z culturemech-id-assigner-v1.0 Assigned CultureMech ID Assigned stable identifier: CultureMech:000740
2026-03-17T06:29:40.000000Z mediaingredientmech-enrichment-v1.0 Added MediaIngredientMech links Linked ingredients to MediaIngredientMech identifiers
2026-03-17T19:45:09.318707+00:00 normalize_media_names_to_snake_case.py NAME_NORMALIZED Normalized name from "CALDIMICROBIUM THIODISMUTANS MEDIUM" to "caldimicrobium_thiodismutans_medium" for consistent matching
2026-05-20T06:01:10.689874+00:00 migrate_ph_range.py MIGRATED_PH_RANGE ph_range '7.5-8' -> {'min': 7.5, 'max': 8.0}
2026-06-05T07:40:52.175976+00:00 mim-legacy-to-chebi-migration-v1.0 Refreshed legacy MediaIngredientMech links to CHEBI keying Replaced 27 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme.
2026-06-10T07:46:54.177809+00:00 primary-term-label-canonicalization-v1.0 Canonicalized primary term label to OBO canonical (OAK) 4 primary term(s); ingredient name resolved by OAK sqlite:obo:chebi.