Overview
- External ontology
- mediadive.medium:1284 (source link unavailable) — GRANULICELLA MEDIUM
- Applications
-
- Microbial cultivation
- pH range
- {'min': 4.5, 'max': 5.2}
- Notes
- Source: DSMZ | Link: https://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium1284.pdf
Composition
flowchart LR
CultureMech_000748["granulicella_medium"]
ing0_Glucose["Glucose"]
CultureMech_000748 --> ing0_Glucose
CHEBI_17234["CHEBI:17234"]
ing0_Glucose -.-> CHEBI_17234
ing1_Yeast_extract["Yeast extract"]
CultureMech_000748 --> ing1_Yeast_extract
ing2_Casamino_acids["Casamino acids"]
CultureMech_000748 --> ing2_Casamino_acids
ing3_MgSO4_x_7_H2O["MgSO4 x 7 H2O"]
CultureMech_000748 --> ing3_MgSO4_x_7_H2O
CHEBI_31795["CHEBI:31795"]
ing3_MgSO4_x_7_H2O -.-> CHEBI_31795
ing4_CaCl2_x_2_H2O["CaCl2 x 2 H2O"]
CultureMech_000748 --> ing4_CaCl2_x_2_H2O
CHEBI_86158["CHEBI:86158"]
ing4_CaCl2_x_2_H2O -.-> CHEBI_86158
ing5_Agar["Agar"]
CultureMech_000748 --> ing5_Agar
CHEBI_2509["CHEBI:2509"]
ing5_Agar -.-> CHEBI_2509
Ingredients (6)
| # | Ingredient | Concentration | Ontology | Notes |
|---|---|---|---|---|
| 1 |
Glucose
C6H12O6 180.156 g/mol
|
0.5 G_PER_L |
CHEBI:17234
glucose
|
|
| 2 | Yeast extract | 0.1 G_PER_L | ||
| 3 | Casamino acids | 0.1 G_PER_L | ||
| 4 |
MgSO4 x 7 H2O
7H2O.Mg.O4S 246.473 g/mol
|
0.04 G_PER_L |
CHEBI:31795
magnesium sulfate heptahydrate
|
|
| 5 |
CaCl2 x 2 H2O
Ca.2Cl.2H2O 147.014 g/mol
|
0.02 G_PER_L |
CHEBI:86158
calcium chloride dihydrate
|
|
| 6 | Agar | 15 G_PER_L |
CHEBI:2509
agar
|
(for solid medium) |
Preparation
- The medium may be solidified with 15 g/l agar. Adjust to pH 4.5 - 5.2.
Target organisms
No target organisms are recorded.
Provenance
| Timestamp | Curator | Action | Notes |
|---|---|---|---|
| 2026-01-26T17:53:13.996339Z | mediadive-import | Imported from MediaDive | Source: DSMZ, ID: 1284 |
| 2026-01-28T19:48:23.548607Z | chebi-enrichment | Added CHEBI ontology terms to ingredients | Enriched using MicrobeMediaParam and MediaDive chemical mappings |
| 2026-03-13T06:18:58.052606Z | culturemech-id-assigner-v1.0 | Assigned CultureMech ID | Assigned stable identifier: CultureMech:000748 |
| 2026-03-17T06:30:15.000000Z | mediaingredientmech-enrichment-v1.0 | Added MediaIngredientMech links | Linked ingredients to MediaIngredientMech identifiers |
| 2026-03-17T19:45:09.404501+00:00 | normalize_media_names_to_snake_case.py | NAME_NORMALIZED | Normalized name from "GRANULICELLA MEDIUM" to "granulicella_medium" for consistent matching |
| 2026-05-20T06:01:21.330467+00:00 | migrate_ph_range.py | MIGRATED_PH_RANGE | ph_range '4.5-5.2' -> {'min': 4.5, 'max': 5.2} |
| 2026-06-05T07:41:06.691588+00:00 | mim-legacy-to-chebi-migration-v1.0 | Refreshed legacy MediaIngredientMech links to CHEBI keying | Replaced 5 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme. |
| 2026-06-08T23:19:39.383313+00:00 | chebi-regrounding-v1.0 | Corrected CHEBI ingredient grounding (audit G21/G22) | Re-grounded 2 term reference(s) to the correct CHEBI (see reports/chebi_grounding_audit.md). Label-conditional fixes for pyridoxine/pyridoxamine, glycerol, MnSO4, Ca(NO3)2, selenate; casamino acids de-grounded (mixture, no single CHEBI). |
| 2026-10-07T13:32:40.086487+00:00 | codex | CURATED_MEDIUM_CONCENTRATION_SOURCE_EVIDENCE | Exact medium/source membership, ingredient inventory/forms, physical-state conditions, literal snippets, and arithmetic checked. Stock boundaries, source-only rows, preparation context, and variants remain explicit follow-up tasks; secondary chemical mappings unchanged. |