granulicella_paludicola_medium

CultureMech:000749 bacterial COMPLEX SOLID_AGAR
Original name: GRANULICELLA PALUDICOLA MEDIUM

Overview

External ontology
mediadive.medium:1285 (source link unavailable) — GRANULICELLA PALUDICOLA MEDIUM
Applications
  • Microbial cultivation
pH range
{'min': 4.0, 'max': 5.0}
Notes
Source: DSMZ | Link: https://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium1285.pdf

Composition

flowchart LR
    CultureMech_000749["granulicella_paludicola_medium"]
    ing0_Fructose["Fructose"]
    CultureMech_000749 --> ing0_Fructose
    CHEBI_28757["CHEBI:28757"]
    ing0_Fructose -.-> CHEBI_28757
    ing1_Yeast_extract["Yeast extract"]
    CultureMech_000749 --> ing1_Yeast_extract
    ing2_Casamino_acids["Casamino acids"]
    CultureMech_000749 --> ing2_Casamino_acids
    ing3_MgSO4_x_7_H2O["MgSO4 x 7 H2O"]
    CultureMech_000749 --> ing3_MgSO4_x_7_H2O
    CHEBI_31795["CHEBI:31795"]
    ing3_MgSO4_x_7_H2O -.-> CHEBI_31795
    ing4_CaCl2_x_2_H2O["CaCl2 x 2 H2O"]
    CultureMech_000749 --> ing4_CaCl2_x_2_H2O
    CHEBI_86158["CHEBI:86158"]
    ing4_CaCl2_x_2_H2O -.-> CHEBI_86158
    ing5_KH2PO4["KH2PO4"]
    CultureMech_000749 --> ing5_KH2PO4
    CHEBI_63036["CHEBI:63036"]
    ing5_KH2PO4 -.-> CHEBI_63036
    ing6__NH4_2SO4["(NH4)2SO4"]
    CultureMech_000749 --> ing6__NH4_2SO4
    CHEBI_62946["CHEBI:62946"]
    ing6__NH4_2SO4 -.-> CHEBI_62946
    ing7_Agar["Agar"]
    CultureMech_000749 --> ing7_Agar
    CHEBI_2509["CHEBI:2509"]
    ing7_Agar -.-> CHEBI_2509

Ingredients (8)

# Ingredient Concentration Ontology Notes
1 Fructose 0.5 G_PER_L CHEBI:28757
fructose
2 Yeast extract 0.05 G_PER_L
3 Casamino acids 0.05 G_PER_L
4 MgSO4 x 7 H2O
7H2O.Mg.O4S 246.473 g/mol
0.04 G_PER_L CHEBI:31795
magnesium sulfate heptahydrate
5 CaCl2 x 2 H2O
Ca.2Cl.2H2O 147.014 g/mol
0.02 G_PER_L CHEBI:86158
calcium chloride dihydrate
6 KH2PO4
H2O4P.K 136.084 g/mol
0.1 G_PER_L CHEBI:63036
potassium dihydrogen phosphate
7 (NH4)2SO4
2H4N.O4S 132.141 g/mol
0.1 G_PER_L CHEBI:62946
ammonium sulfate
8 Agar 15 G_PER_L CHEBI:2509
agar
 (for solid medium)

Preparation

  1. The medium may be solidified with 15 g/l agar. Adjust to pH 4.0 - 5.0 with alginic acid.

Target organisms

No target organisms are recorded.

Provenance

TimestampCuratorActionNotes
2026-01-26T17:53:13.997866Z mediadive-import Imported from MediaDive Source: DSMZ, ID: 1285
2026-01-28T19:48:28.300978Z chebi-enrichment Added CHEBI ontology terms to ingredients Enriched using MicrobeMediaParam and MediaDive chemical mappings
2026-03-13T06:18:58.057296Z culturemech-id-assigner-v1.0 Assigned CultureMech ID Assigned stable identifier: CultureMech:000749
2026-03-17T06:30:25.000000Z mediaingredientmech-enrichment-v1.0 Added MediaIngredientMech links Linked ingredients to MediaIngredientMech identifiers
2026-03-17T19:45:09.410029+00:00 normalize_media_names_to_snake_case.py NAME_NORMALIZED Normalized name from "GRANULICELLA PALUDICOLA MEDIUM" to "granulicella_paludicola_medium" for consistent matching
2026-05-20T06:01:21.337148+00:00 migrate_ph_range.py MIGRATED_PH_RANGE ph_range '4-5' -> {'min': 4.0, 'max': 5.0}
2026-06-05T07:41:06.697747+00:00 mim-legacy-to-chebi-migration-v1.0 Refreshed legacy MediaIngredientMech links to CHEBI keying Replaced 7 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme.
2026-06-08T23:19:39.389624+00:00 chebi-regrounding-v1.0 Corrected CHEBI ingredient grounding (audit G21/G22) Re-grounded 2 term reference(s) to the correct CHEBI (see reports/chebi_grounding_audit.md). Label-conditional fixes for pyridoxine/pyridoxamine, glycerol, MnSO4, Ca(NO3)2, selenate; casamino acids de-grounded (mixture, no single CHEBI).
2026-06-10T07:46:59.174655+00:00 primary-term-label-canonicalization-v1.0 Canonicalized primary term label to OBO canonical (OAK) 2 primary term(s); ingredient name resolved by OAK sqlite:obo:chebi.
2026-10-07T13:32:40.086487+00:00 codex CURATED_MEDIUM_CONCENTRATION_SOURCE_EVIDENCE Exact medium/source membership, ingredient inventory/forms, physical-state conditions, literal snippets, and arithmetic checked. Stock boundaries, source-only rows, preparation context, and variants remain explicit follow-up tasks; secondary chemical mappings unchanged.