Overview
- External ontology
- mediadive.medium:1288 (source link unavailable) — RHIZOMICROBIUM MEDIUM
- Applications
-
- Microbial cultivation
- pH
- 6.8
- Notes
- Source: DSMZ | Link: https://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium1288.pdf
Composition
flowchart LR
CultureMech_000751["rhizomicrobium_medium"]
ing0_Trypticase["Trypticase"]
CultureMech_000751 --> ing0_Trypticase
ing1_Yeast_extract["Yeast extract"]
CultureMech_000751 --> ing1_Yeast_extract
ing2_K2HPO4["K2HPO4"]
CultureMech_000751 --> ing2_K2HPO4
CHEBI_131527["CHEBI:131527"]
ing2_K2HPO4 -.-> CHEBI_131527
ing3_KH2PO4["KH2PO4"]
CultureMech_000751 --> ing3_KH2PO4
CHEBI_63036["CHEBI:63036"]
ing3_KH2PO4 -.-> CHEBI_63036
ing4__NH4_2SO4["(NH4)2SO4"]
CultureMech_000751 --> ing4__NH4_2SO4
CHEBI_62946["CHEBI:62946"]
ing4__NH4_2SO4 -.-> CHEBI_62946
ing5_NaCl["NaCl"]
CultureMech_000751 --> ing5_NaCl
CHEBI_26710["CHEBI:26710"]
ing5_NaCl -.-> CHEBI_26710
ing6_MgSO4_x_7_H2O["MgSO4 x 7 H2O"]
CultureMech_000751 --> ing6_MgSO4_x_7_H2O
CHEBI_31795["CHEBI:31795"]
ing6_MgSO4_x_7_H2O -.-> CHEBI_31795
ing7_CaCl2_x_2_H2O["CaCl2 x 2 H2O"]
CultureMech_000751 --> ing7_CaCl2_x_2_H2O
CHEBI_86158["CHEBI:86158"]
ing7_CaCl2_x_2_H2O -.-> CHEBI_86158
ing8_Na2CO3["Na2CO3"]
CultureMech_000751 --> ing8_Na2CO3
CHEBI_29377["CHEBI:29377"]
ing8_Na2CO3 -.-> CHEBI_29377
ing9_L_Cysteine_HCl["L-Cysteine HCl"]
CultureMech_000751 --> ing9_L_Cysteine_HCl
CHEBI_91247["CHEBI:91247"]
ing9_L_Cysteine_HCl -.-> CHEBI_91247
ing10_Resazurin["Resazurin"]
CultureMech_000751 --> ing10_Resazurin
CHEBI_8806["CHEBI:8806"]
ing10_Resazurin -.-> CHEBI_8806
ing11_Glucose["Glucose"]
CultureMech_000751 --> ing11_Glucose
CHEBI_17234["CHEBI:17234"]
ing11_Glucose -.-> CHEBI_17234
ing12_Cellobiose["Cellobiose"]
CultureMech_000751 --> ing12_Cellobiose
CHEBI_17057["CHEBI:17057"]
ing12_Cellobiose -.-> CHEBI_17057
ing13_Maltose["Maltose"]
CultureMech_000751 --> ing13_Maltose
CHEBI_17306["CHEBI:17306"]
ing13_Maltose -.-> CHEBI_17306
ing14_Starch["Starch"]
CultureMech_000751 --> ing14_Starch
CHEBI_28017["CHEBI:28017"]
ing14_Starch -.-> CHEBI_28017
Ingredients (15)
| # | Ingredient | Concentration | Ontology | Notes |
|---|---|---|---|---|
| 1 | Trypticase | 10 G_PER_L |
MICRO:0000175 (source link unavailable)
Trypticase peptone
|
|
| 2 | Yeast extract | 5 G_PER_L | ||
| 3 |
K2HPO4
HO4P.2K 174.174 g/mol
|
0.45 G_PER_L |
CHEBI:131527
dipotassium hydrogen phosphate
|
|
| 4 |
KH2PO4
H2O4P.K 136.084 g/mol
|
0.45 G_PER_L |
CHEBI:63036
potassium dihydrogen phosphate
|
|
| 5 |
(NH4)2SO4
2H4N.O4S 132.141 g/mol
|
0.9 G_PER_L |
CHEBI:62946
ammonium sulfate
|
|
| 6 |
NaCl
Cl.Na 58.443 g/mol
|
0.9 G_PER_L |
CHEBI:26710
sodium chloride
|
|
| 7 |
MgSO4 x 7 H2O
7H2O.Mg.O4S 246.473 g/mol
|
0.09 G_PER_L |
CHEBI:31795
magnesium sulfate heptahydrate
|
|
| 8 |
CaCl2 x 2 H2O
Ca.2Cl.2H2O 147.014 g/mol
|
0.09 G_PER_L |
CHEBI:86158
calcium chloride dihydrate
|
|
| 9 |
Na2CO3
CO3.2Na 105.988 g/mol
|
0.2 G_PER_L |
CHEBI:29377
sodium carbonate
|
|
| 10 |
L-Cysteine HCl
C3H7NO2S.HCl 157.622 g/mol
|
0.3 G_PER_L |
CHEBI:91247
L-cysteine hydrochloride
|
|
| 11 |
Resazurin
C12H7NO4 229.191 g/mol
|
0.001 G_PER_L |
CHEBI:8806
Resazurin
|
|
| 12 |
Glucose
C6H12O6 180.156 g/mol
|
0.25 G_PER_L |
CHEBI:17234
glucose
|
|
| 13 |
Cellobiose
C12H22O11 342.297 g/mol
|
0.25 G_PER_L |
CHEBI:17057
cellobiose
|
|
| 14 |
Maltose
C12H22O11 342.297 g/mol
|
0.25 G_PER_L |
CHEBI:17306
maltose
|
|
| 15 | Starch | 0.25 G_PER_L |
CHEBI:28017
starch
|
Preparation
- Prepare the medium without glucose, cellobiose, maltose, and soluble starch. Boil the medium and cool under an atmosphere of N2:CO2 (80:20) and adjust the pH to 6.8. Dispense into Hungate tubes or serum bottles under the same gas atmosphere and seal with rubber stoppers. Add the glucose, cellobiose, maltose, and soluble starch from N2/CO2 gassed and sterile-filtered stock solutions (pH 6.8).
Target organisms
No target organisms are recorded.
Provenance
| Timestamp | Curator | Action | Notes |
|---|---|---|---|
| 2026-01-26T17:53:14.001262Z | mediadive-import | Imported from MediaDive | Source: DSMZ, ID: 1288 |
| 2026-01-28T19:48:21.756055Z | chebi-enrichment | Added CHEBI ontology terms to ingredients | Enriched using MicrobeMediaParam and MediaDive chemical mappings |
| 2026-03-13T06:18:58.068400Z | culturemech-id-assigner-v1.0 | Assigned CultureMech ID | Assigned stable identifier: CultureMech:000751 |
| 2026-03-17T06:30:11.000000Z | mediaingredientmech-enrichment-v1.0 | Added MediaIngredientMech links | Linked ingredients to MediaIngredientMech identifiers |
| 2026-03-17T19:45:09.423530+00:00 | normalize_media_names_to_snake_case.py | NAME_NORMALIZED | Normalized name from "RHIZOMICROBIUM MEDIUM" to "rhizomicrobium_medium" for consistent matching |
| 2026-06-05T07:41:44.222037+00:00 | mim-legacy-to-chebi-migration-v1.0 | Refreshed legacy MediaIngredientMech links to CHEBI keying | Replaced 12 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme. |
| 2026-06-10T07:47:10.913351+00:00 | primary-term-label-canonicalization-v1.0 | Canonicalized primary term label to OBO canonical (OAK) | 4 primary term(s); ingredient name resolved by OAK sqlite:obo:chebi. |
| 2026-09-22T00:03:26.686152+00:00 | repair_rejected_peptone_groundings.py | CORRECTED_REJECTED_PEPTONE_GROUNDING | CultureMech #484. /ingredients/0/term/id: Trypticase CHEBI:78018 -> MICRO:0000175 (replace_term). Reviewed identities: https://github.com/CultureBotAI/MediaIngredientMech/tree/24562493919391df4468a4285b7c997960cc8868. Publication resolver: MIM label index 9f09e4fb97fb0e6cbbd6f25baca40b36512adb88. MediaDive source IDs and concentrations preserved. |
| 2026-10-07T13:32:40.086487+00:00 | codex | CURATED_MEDIUM_CONCENTRATION_SOURCE_EVIDENCE | Exact medium/source membership, ingredient inventory/forms, physical-state conditions, literal snippets, and arithmetic checked. Stock boundaries, source-only rows, preparation context, and variants remain explicit follow-up tasks; secondary chemical mappings unchanged. |