chthonomonas_medium

CultureMech:000754 bacterial COMPLEX LIQUID
Original name: CHTHONOMONAS MEDIUM

Overview

External ontology
mediadive.medium:1291 (source link unavailable) — CHTHONOMONAS MEDIUM
Applications
  • Microbial cultivation
pH range
{'min': 5.0, 'max': 5.3}
Notes
Source: DSMZ | Link: https://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium1291.pdf

Composition

flowchart LR
    CultureMech_000754["chthonomonas_medium"]
    ing0_Gellan_Gum["Gellan Gum"]
    CultureMech_000754 --> ing0_Gellan_Gum
    CHEBI_85248["CHEBI:85248"]
    ing0_Gellan_Gum -.-> CHEBI_85248
    ing1__NH4_2SO4["(NH4)2SO4"]
    CultureMech_000754 --> ing1__NH4_2SO4
    CHEBI_62946["CHEBI:62946"]
    ing1__NH4_2SO4 -.-> CHEBI_62946
    ing2_K2HPO4["K2HPO4"]
    CultureMech_000754 --> ing2_K2HPO4
    CHEBI_131527["CHEBI:131527"]
    ing2_K2HPO4 -.-> CHEBI_131527
    ing3_CaCl2_x_2_H2O["CaCl2 x 2 H2O"]
    CultureMech_000754 --> ing3_CaCl2_x_2_H2O
    CHEBI_86158["CHEBI:86158"]
    ing3_CaCl2_x_2_H2O -.-> CHEBI_86158
    ing4_MgCl2_x_6_H2O["MgCl2 x 6 H2O"]
    CultureMech_000754 --> ing4_MgCl2_x_6_H2O
    CHEBI_86345["CHEBI:86345"]
    ing4_MgCl2_x_6_H2O -.-> CHEBI_86345
    ing5_Yeast_extract["Yeast extract"]
    CultureMech_000754 --> ing5_Yeast_extract
    ing6_Na2_EDTA_x_2_H2O["Na2-EDTA x 2 H2O"]
    CultureMech_000754 --> ing6_Na2_EDTA_x_2_H2O
    CHEBI_64758["CHEBI:64758"]
    ing6_Na2_EDTA_x_2_H2O -.-> CHEBI_64758
    ing7_ZnCl2["ZnCl2"]
    CultureMech_000754 --> ing7_ZnCl2
    CHEBI_49976["CHEBI:49976"]
    ing7_ZnCl2 -.-> CHEBI_49976
    ing8_MnCl2_x_4_H2O["MnCl2 x 4 H2O"]
    CultureMech_000754 --> ing8_MnCl2_x_4_H2O
    CHEBI_86368["CHEBI:86368"]
    ing8_MnCl2_x_4_H2O -.-> CHEBI_86368
    ing9_H3BO3["H3BO3"]
    CultureMech_000754 --> ing9_H3BO3
    CHEBI_33118["CHEBI:33118"]
    ing9_H3BO3 -.-> CHEBI_33118
    ing10_CoCl2_x_6_H2O["CoCl2 x 6 H2O"]
    CultureMech_000754 --> ing10_CoCl2_x_6_H2O
    CHEBI_53503["CHEBI:53503"]
    ing10_CoCl2_x_6_H2O -.-> CHEBI_53503
    ing11_CuCl2_x_2_H2O["CuCl2 x 2 H2O"]
    CultureMech_000754 --> ing11_CuCl2_x_2_H2O
    CHEBI_86318["CHEBI:86318"]
    ing11_CuCl2_x_2_H2O -.-> CHEBI_86318
    ing12_NiCl2_x_6_H2O["NiCl2 x 6 H2O"]
    CultureMech_000754 --> ing12_NiCl2_x_6_H2O
    CHEBI_34887["CHEBI:34887"]
    ing12_NiCl2_x_6_H2O -.-> CHEBI_34887
    ing13_Na2MoO4_x_2_H2O["Na2MoO4 x 2 H2O"]
    CultureMech_000754 --> ing13_Na2MoO4_x_2_H2O
    CHEBI_75213["CHEBI:75213"]
    ing13_Na2MoO4_x_2_H2O -.-> CHEBI_75213
    ing14_p_Aminobenzoic_acid["p-Aminobenzoic acid"]
    CultureMech_000754 --> ing14_p_Aminobenzoic_acid
    CHEBI_30753["CHEBI:30753"]
    ing14_p_Aminobenzoic_acid -.-> CHEBI_30753
    ing15_D_____biotin["D-(+)-biotin"]
    CultureMech_000754 --> ing15_D_____biotin
    CHEBI_15956["CHEBI:15956"]
    ing15_D_____biotin -.-> CHEBI_15956
    ing16_Calcium_pantothenate["Calcium pantothenate"]
    CultureMech_000754 --> ing16_Calcium_pantothenate
    CHEBI_31345["CHEBI:31345"]
    ing16_Calcium_pantothenate -.-> CHEBI_31345

Ingredients (17)

# Ingredient Concentration Ontology Notes
1 Gellan Gum 0.5 G_PER_L CHEBI:85248
gellan gum
2 (NH4)2SO4
2H4N.O4S 132.141 g/mol
4 G_PER_L CHEBI:62946
ammonium sulfate
3 K2HPO4
HO4P.2K 174.174 g/mol
0.45 G_PER_L CHEBI:131527
dipotassium hydrogen phosphate
4 CaCl2 x 2 H2O
Ca.2Cl.2H2O 147.014 g/mol
0.15 G_PER_L CHEBI:86158
calcium chloride dihydrate
5 MgCl2 x 6 H2O
2Cl.6H2O.Mg 203.301 g/mol
1 G_PER_L CHEBI:86345
magnesium dichloride hexahydrate
6 Yeast extract 0.01 G_PER_L
7 Na2-EDTA x 2 H2O
C10H14N2O8.2H2O.2Na 372.238 g/mol
5.2 G_PER_L CHEBI:64758
EDTA disodium salt dihydrate
8 ZnCl2
2Cl.Zn 136.296 g/mol
0.07 G_PER_L CHEBI:49976
zinc dichloride
9 MnCl2 x 4 H2O
Cl2Mn.4H2O 197.904 g/mol
0.1 G_PER_L CHEBI:86368
manganese(II) chloride tetrahydrate
10 H3BO3
H3BO3 61.833 g/mol
0.006 G_PER_L CHEBI:33118
boric acid
11 CoCl2 x 6 H2O
2Cl.Co.6H2O 237.929 g/mol
0.19 G_PER_L CHEBI:53503
cobalt chloride hexahydrate
12 CuCl2 x 2 H2O
Cl2Cu.2H2O 170.482 g/mol
0.002 G_PER_L CHEBI:86318
copper(II) chloride dihydrate
13 NiCl2 x 6 H2O
Cl2Ni 129.599 g/mol
0.024 G_PER_L CHEBI:34887
nickel dichloride
14 Na2MoO4 x 2 H2O
2H2O.MoO4.2Na 241.946 g/mol
0.036 G_PER_L CHEBI:75213
sodium molybdate dihydrate
15 p-Aminobenzoic acid
C7H7NO2 137.138 g/mol
0.08 G_PER_L CHEBI:30753
4-aminobenzoic acid
16 D-(+)-biotin
C10H16N2O3S 244.316 g/mol
0.02 G_PER_L CHEBI:15956
biotin
17 Calcium pantothenate
2C9H16NO5.Ca 476.536 g/mol
0.1 G_PER_L CHEBI:31345
Calcium pantothenate

Preparation

  1. Add gellan gum and boil to dissolve. Then add the remaining ingredients (except magnesium chloride, yeast extract and vitamins), adjust the pH value to 5.0 to 5.3 and fill the medium under an air atmosphere into tubes of the Hungate type or similar vials up to 30% their volume. After autoclaving add magnesium chloride, yeast extract and vitamins from steril stock solutions. Vitamins should be sterilized by filtration.
  2. Note: Incubation with shaking may improve cell yield.
  3. Dissolve EDTA in 800 ml distilled water, adjust pH to 7 using 2 N NaOH and add ferrous chloride. After ferrous chloride has dissolved add other compounds. Finally adjust pH to 6.0 and bring volume to 1000 ml.

Solutions

Trace element solution SL-11

Dose: 1 ML_PER_L

Stock composition
IngredientConcentrationNotes
FeCl2 x 4 H2O 1.5 G_PER_L

Seven vitamins solution

Dose: 1 ML_PER_L

Stock composition
IngredientConcentrationNotes
Vitamin B12 0.1 G_PER_L
Nicotinic acid 0.2 G_PER_L
Pyridoxine hydrochloride 0.3 G_PER_L
Thiamine-HCl x 2 H2O 0.2 G_PER_L

Target organisms

No target organisms are recorded.

Provenance

TimestampCuratorActionNotes
2026-01-26T17:53:14.009662Z mediadive-import Imported from MediaDive Source: DSMZ, ID: 1291
2026-03-13T06:18:58.095631Z culturemech-id-assigner-v1.0 Assigned CultureMech ID Assigned stable identifier: CultureMech:000754
2026-03-17T06:30:56.000000Z mediaingredientmech-enrichment-v1.0 Added MediaIngredientMech links Linked ingredients to MediaIngredientMech identifiers
2026-03-17T19:45:09.454134+00:00 normalize_media_names_to_snake_case.py NAME_NORMALIZED Normalized name from "CHTHONOMONAS MEDIUM" to "chthonomonas_medium" for consistent matching
2026-05-20T06:01:11.637055+00:00 migrate_ph_range.py MIGRATED_PH_RANGE ph_range '5-5.3' -> {'min': 5.0, 'max': 5.3}
2026-06-05T07:40:53.634016+00:00 mim-legacy-to-chebi-migration-v1.0 Refreshed legacy MediaIngredientMech links to CHEBI keying Replaced 21 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme.
2026-06-10T07:46:54.591323+00:00 primary-term-label-canonicalization-v1.0 Canonicalized primary term label to OBO canonical (OAK) 3 primary term(s); ingredient name resolved by OAK sqlite:obo:chebi.
2026-08-07T07:05:21.738469+00:00 apply_cocktail_nesting.py NESTED_FLATTENED_COCKTAIL Moved 5 stock-strength component(s) out of ingredients into 2 solution(s): 1 -> 'Trace element solution SL-11' @ 1 ml/l; 4 -> 'Seven vitamins solution' @ 1 ml/l. Each moved component matched the MediaDive stock by name AND value; bulk ingredients sharing a name were left in place (#150).