desulfurispirillum_indicum_medium

CultureMech:000756 bacterial DEFINED LIQUID
Original name: DESULFURISPIRILLUM INDICUM MEDIUM

Overview

External ontology
mediadive.medium:1294 (source link unavailable) — DESULFURISPIRILLUM INDICUM MEDIUM
Applications
  • Microbial cultivation
pH range
{'min': 6.5, 'max': 6.8}
Notes
Source: DSMZ | Link: https://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium1294.pdf

Composition

flowchart LR
    CultureMech_000756["desulfurispirillum_indicum_medium"]
    ing0_NaCl["NaCl"]
    CultureMech_000756 --> ing0_NaCl
    CHEBI_26710["CHEBI:26710"]
    ing0_NaCl -.-> CHEBI_26710
    ing1_KCl["KCl"]
    CultureMech_000756 --> ing1_KCl
    CHEBI_32588["CHEBI:32588"]
    ing1_KCl -.-> CHEBI_32588
    ing2_KH2PO4["KH2PO4"]
    CultureMech_000756 --> ing2_KH2PO4
    CHEBI_63036["CHEBI:63036"]
    ing2_KH2PO4 -.-> CHEBI_63036
    ing3_NH4Cl["NH4Cl"]
    CultureMech_000756 --> ing3_NH4Cl
    CHEBI_31206["CHEBI:31206"]
    ing3_NH4Cl -.-> CHEBI_31206
    ing4_NaNO3["NaNO3"]
    CultureMech_000756 --> ing4_NaNO3
    CHEBI_63005["CHEBI:63005"]
    ing4_NaNO3 -.-> CHEBI_63005
    ing5_CaCl2_x_2_H2O["CaCl2 x 2 H2O"]
    CultureMech_000756 --> ing5_CaCl2_x_2_H2O
    CHEBI_86158["CHEBI:86158"]
    ing5_CaCl2_x_2_H2O -.-> CHEBI_86158
    ing6_MgCl2_x_6_H2O["MgCl2 x 6 H2O"]
    CultureMech_000756 --> ing6_MgCl2_x_6_H2O
    CHEBI_86345["CHEBI:86345"]
    ing6_MgCl2_x_6_H2O -.-> CHEBI_86345
    ing7_Sodium_resazurin["Sodium resazurin"]
    CultureMech_000756 --> ing7_Sodium_resazurin
    CHEBI_8806["CHEBI:8806"]
    ing7_Sodium_resazurin -.-> CHEBI_8806
    ing8_Na2CO3["Na2CO3"]
    CultureMech_000756 --> ing8_Na2CO3
    CHEBI_29377["CHEBI:29377"]
    ing8_Na2CO3 -.-> CHEBI_29377
    ing9_Na_pyruvate["Na-pyruvate"]
    CultureMech_000756 --> ing9_Na_pyruvate
    CHEBI_50144["CHEBI:50144"]
    ing9_Na_pyruvate -.-> CHEBI_50144
    ing10_Na2S_x_9_H2O["Na2S x 9 H2O"]
    CultureMech_000756 --> ing10_Na2S_x_9_H2O
    CHEBI_76209["CHEBI:76209"]
    ing10_Na2S_x_9_H2O -.-> CHEBI_76209
    ing11_HCl["HCl"]
    CultureMech_000756 --> ing11_HCl
    CHEBI_17883["CHEBI:17883"]
    ing11_HCl -.-> CHEBI_17883
    ing12_ZnCl2["ZnCl2"]
    CultureMech_000756 --> ing12_ZnCl2
    CHEBI_49976["CHEBI:49976"]
    ing12_ZnCl2 -.-> CHEBI_49976
    ing13_MnCl2_x_4_H2O["MnCl2 x 4 H2O"]
    CultureMech_000756 --> ing13_MnCl2_x_4_H2O
    CHEBI_86368["CHEBI:86368"]
    ing13_MnCl2_x_4_H2O -.-> CHEBI_86368
    ing14_H3BO3["H3BO3"]
    CultureMech_000756 --> ing14_H3BO3
    CHEBI_33118["CHEBI:33118"]
    ing14_H3BO3 -.-> CHEBI_33118
    ing15_CoCl2_x_6_H2O["CoCl2 x 6 H2O"]
    CultureMech_000756 --> ing15_CoCl2_x_6_H2O
    CHEBI_53503["CHEBI:53503"]
    ing15_CoCl2_x_6_H2O -.-> CHEBI_53503
    ing16_CuCl2_x_2_H2O["CuCl2 x 2 H2O"]
    CultureMech_000756 --> ing16_CuCl2_x_2_H2O
    CHEBI_86318["CHEBI:86318"]
    ing16_CuCl2_x_2_H2O -.-> CHEBI_86318
    ing17_NiCl2_x_6_H2O["NiCl2 x 6 H2O"]
    CultureMech_000756 --> ing17_NiCl2_x_6_H2O
    CHEBI_34887["CHEBI:34887"]
    ing17_NiCl2_x_6_H2O -.-> CHEBI_34887
    ing18_Na2MoO4_x_2_H2O["Na2MoO4 x 2 H2O"]
    CultureMech_000756 --> ing18_Na2MoO4_x_2_H2O
    CHEBI_75213["CHEBI:75213"]
    ing18_Na2MoO4_x_2_H2O -.-> CHEBI_75213
    ing19_NaOH["NaOH"]
    CultureMech_000756 --> ing19_NaOH
    CHEBI_32145["CHEBI:32145"]
    ing19_NaOH -.-> CHEBI_32145
    ing20_Na2SeO3_x_5_H2O["Na2SeO3 x 5 H2O"]
    CultureMech_000756 --> ing20_Na2SeO3_x_5_H2O
    CHEBI_131361["CHEBI:131361"]
    ing20_Na2SeO3_x_5_H2O -.-> CHEBI_131361
    ing21_Na2WO4_x_2_H2O["Na2WO4 x 2 H2O"]
    CultureMech_000756 --> ing21_Na2WO4_x_2_H2O
    CHEBI_63939["CHEBI:63939"]
    ing21_Na2WO4_x_2_H2O -.-> CHEBI_63939
    ing22_Biotin["Biotin"]
    CultureMech_000756 --> ing22_Biotin
    CHEBI_15956["CHEBI:15956"]
    ing22_Biotin -.-> CHEBI_15956
    ing23_Folic_acid["Folic acid"]
    CultureMech_000756 --> ing23_Folic_acid
    CHEBI_27470["CHEBI:27470"]
    ing23_Folic_acid -.-> CHEBI_27470
    ing24_Thiamine_HCl["Thiamine HCl"]
    CultureMech_000756 --> ing24_Thiamine_HCl
    CHEBI_49105["CHEBI:49105"]
    ing24_Thiamine_HCl -.-> CHEBI_49105
    ing25_Riboflavin["Riboflavin"]
    CultureMech_000756 --> ing25_Riboflavin
    CHEBI_17015["CHEBI:17015"]
    ing25_Riboflavin -.-> CHEBI_17015
    ing26_Calcium_D_____pantothenate["Calcium D-(+)-pantothenate"]
    CultureMech_000756 --> ing26_Calcium_D_____pantothenate
    CHEBI_31345["CHEBI:31345"]
    ing26_Calcium_D_____pantothenate -.-> CHEBI_31345
    ing27__DL__alpha_Lipoic_acid["(DL)-alpha-Lipoic acid"]
    CultureMech_000756 --> ing27__DL__alpha_Lipoic_acid
    CHEBI_16494["CHEBI:16494"]
    ing27__DL__alpha_Lipoic_acid -.-> CHEBI_16494
    ing28_D_____biotin["D-(+)-biotin"]
    CultureMech_000756 --> ing28_D_____biotin
    ing28_D_____biotin -.-> CHEBI_15956
    ing29_Calcium_pantothenate["Calcium pantothenate"]
    CultureMech_000756 --> ing29_Calcium_pantothenate
    ing29_Calcium_pantothenate -.-> CHEBI_31345

Ingredients (30)

# Ingredient Concentration Ontology Notes
1 NaCl
Cl.Na 58.443 g/mol
22.9084 G_PER_L CHEBI:26710
sodium chloride
2 KCl
Cl.K 74.551 g/mol
1.29482 G_PER_L CHEBI:32588
potassium chloride
3 KH2PO4
H2O4P.K 136.084 g/mol
0.199203 G_PER_L CHEBI:63036
potassium dihydrogen phosphate
4 NH4Cl
Cl.H4N 53.492 g/mol
0.498008 G_PER_L CHEBI:31206
ammonium chloride
5 NaNO3
NO3.Na 84.994 g/mol
0.896414 G_PER_L CHEBI:63005
sodium nitrate
6 CaCl2 x 2 H2O
Ca.2Cl.2H2O 147.014 g/mol
0.0996016 G_PER_L CHEBI:86158
calcium chloride dihydrate
7 MgCl2 x 6 H2O
2Cl.6H2O.Mg 203.301 g/mol
2.98805 G_PER_L CHEBI:86345
magnesium dichloride hexahydrate
8 Sodium resazurin
C12H7NO4 229.191 g/mol
0.000498008 G_PER_L CHEBI:8806
Resazurin
9 Na2CO3
CO3.2Na 105.988 g/mol
0.996016 G_PER_L CHEBI:29377
sodium carbonate
10 Na-pyruvate
C3H3O3.Na 110.044 g/mol
1.09562 G_PER_L CHEBI:50144
sodium pyruvate
11 Na2S x 9 H2O
9H2O.2Na.S 240.182 g/mol
0.0996016 G_PER_L CHEBI:76209
sodium sulfide nonahydrate
12 HCl
HCl 36.461 g/mol
2.5 G_PER_L CHEBI:17883
hydrogen chloride
13 ZnCl2
2Cl.Zn 136.296 g/mol
0.07 G_PER_L CHEBI:49976
zinc dichloride
14 MnCl2 x 4 H2O
Cl2Mn.4H2O 197.904 g/mol
0.1 G_PER_L CHEBI:86368
manganese(II) chloride tetrahydrate
15 H3BO3
H3BO3 61.833 g/mol
0.006 G_PER_L CHEBI:33118
boric acid
16 CoCl2 x 6 H2O
2Cl.Co.6H2O 237.929 g/mol
0.19 G_PER_L CHEBI:53503
cobalt chloride hexahydrate
17 CuCl2 x 2 H2O
Cl2Cu.2H2O 170.482 g/mol
0.002 G_PER_L CHEBI:86318
copper(II) chloride dihydrate
18 NiCl2 x 6 H2O
Cl2Ni 129.599 g/mol
0.024 G_PER_L CHEBI:34887
nickel dichloride
19 Na2MoO4 x 2 H2O
2H2O.MoO4.2Na 241.946 g/mol
0.036 G_PER_L CHEBI:75213
sodium molybdate dihydrate
20 NaOH
HO.Na 39.997 g/mol
0.5 G_PER_L CHEBI:32145
sodium hydroxide
21 Na2SeO3 x 5 H2O
5H2O.2Na.O3Se 263.012 g/mol
0.003 G_PER_L CHEBI:131361
disodium selenite pentahydrate
22 Na2WO4 x 2 H2O
2H2O.2Na.O4W 329.846 g/mol
0.004 G_PER_L CHEBI:63939
sodium tungstate dihydrate
23 Biotin
C10H16N2O3S 244.316 g/mol
0.02 G_PER_L CHEBI:15956
biotin
24 Folic acid
C19H19N7O6 441.404 g/mol
0.02 G_PER_L CHEBI:27470
folic acid
25 Thiamine HCl
C12H17N4OS.Cl.HCl 337.276 g/mol
0.05 G_PER_L CHEBI:49105
thiamine hydrochloride
26 Riboflavin
C17H20N4O6 376.369 g/mol
0.05 G_PER_L CHEBI:17015
riboflavin
27 Calcium D-(+)-pantothenate
2C9H16NO5.Ca 476.536 g/mol
0.05 G_PER_L CHEBI:31345
Calcium pantothenate
28 (DL)-alpha-Lipoic acid
C8H14O2S2 206.332 g/mol
0.05 G_PER_L CHEBI:16494
lipoic acid
29 D-(+)-biotin
C10H16N2O3S 244.316 g/mol
0.02 G_PER_L CHEBI:15956
biotin
30 Calcium pantothenate
2C9H16NO5.Ca 476.536 g/mol
0.1 G_PER_L CHEBI:31345
Calcium pantothenate

Preparation

  1. Dissolve ingredients (except carbonate, vitamins, pyruvate and sulfide) and sparge medium with 80% N2 and 20% CO2 gas mixture for 30 - 45 min to make it anoxic. Dispense medium under same gas atmosphere into anoxic Hungate-type tubes or serum vials and autoclave. Add vitamins, pyruvate and sulfide form anoxic stock solutions prepared under 100% N2 gas and sterilized by filtration. Add carbonate from a sterile anoxic stock solution prepared under 80% N2 and 20% CO2 gas mixture. Adjust pH of the complete medium to 6.5 - 6.8, if necessary.
  2. First dissolve FeCl2 in the HCl, then dilute in water, add and dissolve the other salts. Finally make up to 1000.00 ml.

Solutions

Trace element solution SL-10

Dose: 1 ML_PER_L

Stock composition
IngredientConcentrationNotes
FeCl2 x 4 H2O 1.5 G_PER_L

Seven vitamins solution

Dose: 1 ML_PER_L

Stock composition
IngredientConcentrationNotes
Thiamine-HCl x 2 H2O 0.2 G_PER_L
Nicotinic acid 0.2 G_PER_L [Merged 2 duplicates: 0.05, 0.2]
p-Aminobenzoic acid 0.08 G_PER_L [Merged 2 duplicates: 0.05, 0.08]
Pyridoxine hydrochloride 0.3 G_PER_L [Merged 2 duplicates: 0.1, 0.3]
Vitamin B12 0.1 G_PER_L [Merged 2 duplicates: 0.001, 0.1]

Wolin's vitamin solution (10x)

Dose: 1 ML_PER_L

Stock composition
IngredientConcentrationNotes
Nicotinic acid 0.05 G_PER_L [Merged 2 duplicates: 0.05, 0.2]
p-Aminobenzoic acid 0.05 G_PER_L [Merged 2 duplicates: 0.05, 0.08]
Pyridoxine hydrochloride 0.1 G_PER_L [Merged 2 duplicates: 0.1, 0.3]
Vitamin B12 0.001 G_PER_L [Merged 2 duplicates: 0.001, 0.1]

Target organisms

No target organisms are recorded.

Provenance

TimestampCuratorActionNotes
2026-01-26T17:53:14.018925Z mediadive-import Imported from MediaDive Source: DSMZ, ID: 1294
2026-02-06T05:10:30.478957Z invalid-chebi-removal Removed 1 invalid CHEBI ID(s) Removed CHEBI IDs with invalid format (7+ digits) from MicrobeMediaParam source data
2026-03-13T03:09:06.234967Z data-quality-cleanup-v1.0 Data quality fixes Merged 4 duplicate ingredient(s)
2026-03-13T06:18:58.123472Z culturemech-id-assigner-v1.0 Assigned CultureMech ID Assigned stable identifier: CultureMech:000756
2026-03-17T06:30:12.000000Z mediaingredientmech-enrichment-v1.0 Added MediaIngredientMech links Linked ingredients to MediaIngredientMech identifiers
2026-03-17T19:45:09.484137+00:00 normalize_media_names_to_snake_case.py NAME_NORMALIZED Normalized name from "DESULFURISPIRILLUM INDICUM MEDIUM" to "desulfurispirillum_indicum_medium" for consistent matching
2026-05-20T06:01:15.183980+00:00 migrate_ph_range.py MIGRATED_PH_RANGE ph_range '6.5-6.8' -> {'min': 6.5, 'max': 6.8}
2026-06-05T07:40:58.229496+00:00 mim-legacy-to-chebi-migration-v1.0 Refreshed legacy MediaIngredientMech links to CHEBI keying Replaced 33 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme.
2026-06-10T07:46:56.198088+00:00 primary-term-label-canonicalization-v1.0 Canonicalized primary term label to OBO canonical (OAK) 5 primary term(s); ingredient name resolved by OAK sqlite:obo:chebi.
2026-08-07T23:42:25.459651+00:00 apply_cocktail_nesting.py NESTED_FLATTENED_COCKTAIL Moved 6 stock-strength component(s) out of ingredients into 3 solution(s): 1 -> 'Trace element solution SL-10' @ 1 ml/l; 1 -> 'Seven vitamins solution' @ 1 ml/l; 0 -> "Wolin's vitamin solution (10x)" @ 1 ml/l. 4 component(s) were SUMMED across stocks by the flattening and are restored to each stock's own value, verified by exact sum: nicotinic acid, p-aminobenzoic acid, pyridoxine hydrochloride, vitamin b12. Each moved component matched the MediaDive stock by name AND value; bulk ingredients sharing a name were left in place (#150).