Overview
- External ontology
- mediadive.medium:1296 (source link unavailable) — M1DG-MEDIUM
- Applications
-
- Microbial cultivation
- pH
- 7.0
- Notes
- Source: DSMZ | Link: https://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium1296.pdf
Composition
flowchart LR
CultureMech_000758["m1dg_medium"]
ing0_Casitone["Casitone"]
CultureMech_000758 --> ing0_Casitone
ing1_Glucose["Glucose"]
CultureMech_000758 --> ing1_Glucose
CHEBI_17234["CHEBI:17234"]
ing1_Glucose -.-> CHEBI_17234
ing2_CaCl2_x_2_H2O["CaCl2 x 2 H2O"]
CultureMech_000758 --> ing2_CaCl2_x_2_H2O
CHEBI_86158["CHEBI:86158"]
ing2_CaCl2_x_2_H2O -.-> CHEBI_86158
ing3_MgSO4_x_7_H2O["MgSO4 x 7 H2O"]
CultureMech_000758 --> ing3_MgSO4_x_7_H2O
CHEBI_31795["CHEBI:31795"]
ing3_MgSO4_x_7_H2O -.-> CHEBI_31795
ing4_Agar["Agar"]
CultureMech_000758 --> ing4_Agar
CHEBI_2509["CHEBI:2509"]
ing4_Agar -.-> CHEBI_2509
Ingredients (5)
| # | Ingredient | Concentration | Ontology | Notes |
|---|---|---|---|---|
| 1 | Casitone | 5 G_PER_L | ||
| 2 |
Glucose
C6H12O6 180.156 g/mol
|
3.5 G_PER_L |
CHEBI:17234
glucose
|
|
| 3 |
CaCl2 x 2 H2O
Ca.2Cl.2H2O 147.014 g/mol
|
0.5 G_PER_L |
CHEBI:86158
calcium chloride dihydrate
|
|
| 4 |
MgSO4 x 7 H2O
7H2O.Mg.O4S 246.473 g/mol
|
2 G_PER_L |
CHEBI:31795
magnesium sulfate heptahydrate
|
|
| 5 | Agar | 14 G_PER_L |
CHEBI:2509
agar
|
Preparation
- Adjust pH to 7.0 with KOH.
Target organisms
No target organisms are recorded.
Provenance
| Timestamp | Curator | Action | Notes |
|---|---|---|---|
| 2026-01-26T17:53:14.025800Z | mediadive-import | Imported from MediaDive | Source: DSMZ, ID: 1296 |
| 2026-03-13T06:18:58.133957Z | culturemech-id-assigner-v1.0 | Assigned CultureMech ID | Assigned stable identifier: CultureMech:000758 |
| 2026-03-17T06:29:41.000000Z | mediaingredientmech-enrichment-v1.0 | Added MediaIngredientMech links | Linked ingredients to MediaIngredientMech identifiers |
| 2026-03-17T19:45:09.497015+00:00 | normalize_media_names_to_snake_case.py | NAME_NORMALIZED | Normalized name from "M1DG-MEDIUM" to "m1dg_medium" for consistent matching |
| 2026-06-05T07:41:11.776823+00:00 | mim-legacy-to-chebi-migration-v1.0 | Refreshed legacy MediaIngredientMech links to CHEBI keying | Replaced 4 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme. |
| 2026-10-07T13:32:40.086487+00:00 | codex | CURATED_MEDIUM_CONCENTRATION_SOURCE_EVIDENCE | Exact medium/source membership, ingredient inventory/forms, physical-state conditions, literal snippets, and arithmetic checked. Stock boundaries, source-only rows, preparation context, and variants remain explicit follow-up tasks; secondary chemical mappings unchanged. |