Overview
- External ontology
- mediadive.medium:1300 (source link unavailable) — ACM-MEDIUM FOR VERMINEPHROBACTER
- Applications
-
- Microbial cultivation
- pH range
- {'min': 7.2, 'max': 7.5}
- Notes
- Source: DSMZ | Link: https://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium1300.pdf
Composition
flowchart LR
CultureMech_000763["acm_medium_for_verminephrobacter"]
ing0_Yeast_extract["Yeast extract"]
CultureMech_000763 --> ing0_Yeast_extract
ing1_Casamino_acids["Casamino acids"]
CultureMech_000763 --> ing1_Casamino_acids
ing2_Na_pyruvate["Na-pyruvate"]
CultureMech_000763 --> ing2_Na_pyruvate
CHEBI_50144["CHEBI:50144"]
ing2_Na_pyruvate -.-> CHEBI_50144
ing3_L_Glutamine["L-Glutamine"]
CultureMech_000763 --> ing3_L_Glutamine
CHEBI_18050["CHEBI:18050"]
ing3_L_Glutamine -.-> CHEBI_18050
ing4_MgSO4_x_7_H2O["MgSO4 x 7 H2O"]
CultureMech_000763 --> ing4_MgSO4_x_7_H2O
CHEBI_31795["CHEBI:31795"]
ing4_MgSO4_x_7_H2O -.-> CHEBI_31795
ing5_MOPS["MOPS"]
CultureMech_000763 --> ing5_MOPS
CHEBI_39074["CHEBI:39074"]
ing5_MOPS -.-> CHEBI_39074
ing6_KH2PO4["KH2PO4"]
CultureMech_000763 --> ing6_KH2PO4
CHEBI_63036["CHEBI:63036"]
ing6_KH2PO4 -.-> CHEBI_63036
Ingredients (7)
| # | Ingredient | Concentration | Ontology | Notes |
|---|---|---|---|---|
| 1 | Yeast extract | 0.555556 G_PER_L | ||
| 2 | Casamino acids | 0.222222 G_PER_L | ||
| 3 |
Na-pyruvate
C3H3O3.Na 110.044 g/mol
|
0.555556 G_PER_L |
CHEBI:50144
sodium pyruvate
|
|
| 4 |
L-Glutamine
C5H10N2O3 146.146 g/mol
|
0.555556 G_PER_L |
CHEBI:18050
L-glutamine
|
|
| 5 |
MgSO4 x 7 H2O
7H2O.Mg.O4S 246.473 g/mol
|
0.333333 G_PER_L |
CHEBI:31795
magnesium sulfate heptahydrate
|
|
| 6 | MOPS | 2.22222 G_PER_L |
CHEBI:39074
MOPS
|
|
| 7 |
KH2PO4
H2O4P.K 136.084 g/mol
|
3 G_PER_L |
CHEBI:63036
potassium dihydrogen phosphate
|
Preparation
- Autoclave solutions A and B separately and combine after cooling. Adjust pH to 7.2 -7.5.
- Add Phytagel instead of agar to solidify medium if necessary. Verminephrobacter strains did not grow on plates in our hands.
Target organisms
No target organisms are recorded.
Provenance
| Timestamp | Curator | Action | Notes |
|---|---|---|---|
| 2026-01-26T17:53:14.039614Z | mediadive-import | Imported from MediaDive | Source: DSMZ, ID: 1300 |
| 2026-01-28T19:48:32.854454Z | chebi-enrichment | Added CHEBI ontology terms to ingredients | Enriched using MicrobeMediaParam and MediaDive chemical mappings |
| 2026-03-13T06:18:58.179028Z | culturemech-id-assigner-v1.0 | Assigned CultureMech ID | Assigned stable identifier: CultureMech:000763 |
| 2026-03-17T06:30:34.000000Z | mediaingredientmech-enrichment-v1.0 | Added MediaIngredientMech links | Linked ingredients to MediaIngredientMech identifiers |
| 2026-03-17T19:45:09.547190+00:00 | normalize_media_names_to_snake_case.py | NAME_NORMALIZED | Normalized name from "ACM-MEDIUM FOR VERMINEPHROBACTER" to "acm_medium_for_verminephrobacter" for consistent matching |
| 2026-05-20T06:01:07.579578+00:00 | migrate_ph_range.py | MIGRATED_PH_RANGE | ph_range '7.2-7.5' -> {'min': 7.2, 'max': 7.5} |
| 2026-06-05T07:40:47.470562+00:00 | mim-legacy-to-chebi-migration-v1.0 | Refreshed legacy MediaIngredientMech links to CHEBI keying | Replaced 6 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme. |
| 2026-06-08T23:19:35.518481+00:00 | chebi-regrounding-v1.0 | Corrected CHEBI ingredient grounding (audit G21/G22) | Re-grounded 2 term reference(s) to the correct CHEBI (see reports/chebi_grounding_audit.md). Label-conditional fixes for pyridoxine/pyridoxamine, glycerol, MnSO4, Ca(NO3)2, selenate; casamino acids de-grounded (mixture, no single CHEBI). |
| 2026-06-10T07:46:52.660747+00:00 | primary-term-label-canonicalization-v1.0 | Canonicalized primary term label to OBO canonical (OAK) | 2 primary term(s); ingredient name resolved by OAK sqlite:obo:chebi. |