Overview
- External ontology
- mediadive.medium:1308 (source link unavailable) — PY4S
- Applications
-
- Microbial cultivation
- pH
- 7.3
- Notes
- Source: DSMZ | Link: https://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium1308.pdf
Composition
flowchart LR
CultureMech_000770["py4s"]
ing0_Trypticase_peptone["Trypticase peptone"]
CultureMech_000770 --> ing0_Trypticase_peptone
ing1_Yeast_extract["Yeast extract"]
CultureMech_000770 --> ing1_Yeast_extract
ing2_Glucose["Glucose"]
CultureMech_000770 --> ing2_Glucose
CHEBI_17234["CHEBI:17234"]
ing2_Glucose -.-> CHEBI_17234
ing3_Cellobiose["Cellobiose"]
CultureMech_000770 --> ing3_Cellobiose
CHEBI_17057["CHEBI:17057"]
ing3_Cellobiose -.-> CHEBI_17057
ing4_Maltose["Maltose"]
CultureMech_000770 --> ing4_Maltose
CHEBI_17306["CHEBI:17306"]
ing4_Maltose -.-> CHEBI_17306
ing5_Starch["Starch"]
CultureMech_000770 --> ing5_Starch
CHEBI_28017["CHEBI:28017"]
ing5_Starch -.-> CHEBI_28017
ing6_L_Cysteine_HCl_x_H2O["L-Cysteine HCl x H2O"]
CultureMech_000770 --> ing6_L_Cysteine_HCl_x_H2O
CHEBI_91248["CHEBI:91248"]
ing6_L_Cysteine_HCl_x_H2O -.-> CHEBI_91248
ing7_Sodium_resazurin["Sodium resazurin"]
CultureMech_000770 --> ing7_Sodium_resazurin
CHEBI_8806["CHEBI:8806"]
ing7_Sodium_resazurin -.-> CHEBI_8806
ing8_Na2CO3["Na2CO3"]
CultureMech_000770 --> ing8_Na2CO3
CHEBI_29377["CHEBI:29377"]
ing8_Na2CO3 -.-> CHEBI_29377
ing9_Vitamin_B12["Vitamin B12"]
CultureMech_000770 --> ing9_Vitamin_B12
CHEBI_176843["CHEBI:176843"]
ing9_Vitamin_B12 -.-> CHEBI_176843
ing10_K2HPO4["K2HPO4"]
CultureMech_000770 --> ing10_K2HPO4
CHEBI_131527["CHEBI:131527"]
ing10_K2HPO4 -.-> CHEBI_131527
ing11_CaCl2_x_2_H2O["CaCl2 x 2 H2O"]
CultureMech_000770 --> ing11_CaCl2_x_2_H2O
CHEBI_86158["CHEBI:86158"]
ing11_CaCl2_x_2_H2O -.-> CHEBI_86158
ing12_MgSO4_x_7_H2O["MgSO4 x 7 H2O"]
CultureMech_000770 --> ing12_MgSO4_x_7_H2O
CHEBI_31795["CHEBI:31795"]
ing12_MgSO4_x_7_H2O -.-> CHEBI_31795
ing13_KH2PO4["KH2PO4"]
CultureMech_000770 --> ing13_KH2PO4
CHEBI_63036["CHEBI:63036"]
ing13_KH2PO4 -.-> CHEBI_63036
ing14__NH4_2SO4["(NH4)2SO4"]
CultureMech_000770 --> ing14__NH4_2SO4
CHEBI_62946["CHEBI:62946"]
ing14__NH4_2SO4 -.-> CHEBI_62946
ing15_NaCl["NaCl"]
CultureMech_000770 --> ing15_NaCl
CHEBI_26710["CHEBI:26710"]
ing15_NaCl -.-> CHEBI_26710
Ingredients (16)
| # | Ingredient | Concentration | Ontology | Notes |
|---|---|---|---|---|
| 1 | Trypticase peptone | 10 G_PER_L | ||
| 2 | Yeast extract | 5 G_PER_L | ||
| 3 |
Glucose
C6H12O6 180.156 g/mol
|
0.25 G_PER_L |
CHEBI:17234
glucose
|
|
| 4 |
Cellobiose
C12H22O11 342.297 g/mol
|
0.25 G_PER_L |
CHEBI:17057
cellobiose
|
|
| 5 |
Maltose
C12H22O11 342.297 g/mol
|
0.25 G_PER_L |
CHEBI:17306
maltose
|
|
| 6 | Starch | 0.25 G_PER_L |
CHEBI:28017
starch
|
|
| 7 |
L-Cysteine HCl x H2O
C3H7NO2S.H2O.HCl 175.637 g/mol
|
0.5 G_PER_L |
CHEBI:91248
L-cysteine hydrochloride hydrate
|
|
| 8 |
Sodium resazurin
C12H7NO4 229.191 g/mol
|
0.001 G_PER_L |
CHEBI:8806
Resazurin
|
|
| 9 |
Na2CO3
CO3.2Na 105.988 g/mol
|
0.2 G_PER_L |
CHEBI:29377
sodium carbonate
|
|
| 10 | Vitamin B12 | 1e-05 G_PER_L |
CHEBI:176843
vitamin B12
|
|
| 11 |
K2HPO4
HO4P.2K 174.174 g/mol
|
6 G_PER_L |
CHEBI:131527
dipotassium hydrogen phosphate
|
|
| 12 |
CaCl2 x 2 H2O
Ca.2Cl.2H2O 147.014 g/mol
|
1.2 G_PER_L |
CHEBI:86158
calcium chloride dihydrate
|
|
| 13 |
MgSO4 x 7 H2O
7H2O.Mg.O4S 246.473 g/mol
|
1.2 G_PER_L |
CHEBI:31795
magnesium sulfate heptahydrate
|
|
| 14 |
KH2PO4
H2O4P.K 136.084 g/mol
|
6 G_PER_L |
CHEBI:63036
potassium dihydrogen phosphate
|
|
| 15 |
(NH4)2SO4
2H4N.O4S 132.141 g/mol
|
12 G_PER_L |
CHEBI:62946
ammonium sulfate
|
|
| 16 |
NaCl
Cl.Na 58.443 g/mol
|
12 G_PER_L |
CHEBI:26710
sodium chloride
|
Preparation
- The cysteine, Na2CO3, and vitamin B12 are added after the medium has been boiled and cooled under CO2. Adjust pH to 7.3 Distribute under N2 and autoclave.
Target organisms
No target organisms are recorded.
Provenance
| Timestamp | Curator | Action | Notes |
|---|---|---|---|
| 2026-01-26T17:53:14.060860Z | mediadive-import | Imported from MediaDive | Source: DSMZ, ID: 1308 |
| 2026-03-13T06:18:58.237852Z | culturemech-id-assigner-v1.0 | Assigned CultureMech ID | Assigned stable identifier: CultureMech:000770 |
| 2026-03-17T06:30:09.000000Z | mediaingredientmech-enrichment-v1.0 | Added MediaIngredientMech links | Linked ingredients to MediaIngredientMech identifiers |
| 2026-03-17T19:45:09.614098+00:00 | normalize_media_names_to_snake_case.py | NAME_NORMALIZED | Normalized name from "PY4S" to "py4s" for consistent matching |
| 2026-06-05T07:41:42.875884+00:00 | mim-legacy-to-chebi-migration-v1.0 | Refreshed legacy MediaIngredientMech links to CHEBI keying | Replaced 13 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme. |
| 2026-06-10T07:47:10.528664+00:00 | primary-term-label-canonicalization-v1.0 | Canonicalized primary term label to OBO canonical (OAK) | 4 primary term(s); ingredient name resolved by OAK sqlite:obo:chebi. |