bacillus_locisalis_medium

CultureMech:000771 bacterial COMPLEX SOLID_AGAR
Original name: BACILLUS LOCISALIS MEDIUM

Overview

External ontology
mediadive.medium:1309 (source link unavailable) — BACILLUS LOCISALIS MEDIUM
Applications
  • Microbial cultivation
Notes
Source: DSMZ | Link: https://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium1309.pdf

Composition

flowchart LR
    CultureMech_000771["bacillus_locisalis_medium"]
    ing0_Peptone["Peptone"]
    CultureMech_000771 --> ing0_Peptone
    ing1_Yeast_extract["Yeast extract"]
    CultureMech_000771 --> ing1_Yeast_extract
    ing2_Glucose["Glucose"]
    CultureMech_000771 --> ing2_Glucose
    CHEBI_17234["CHEBI:17234"]
    ing2_Glucose -.-> CHEBI_17234
    ing3_K2HPO4["K2HPO4"]
    CultureMech_000771 --> ing3_K2HPO4
    CHEBI_131527["CHEBI:131527"]
    ing3_K2HPO4 -.-> CHEBI_131527
    ing4_MgSO4_x_7_H2O["MgSO4 x 7 H2O"]
    CultureMech_000771 --> ing4_MgSO4_x_7_H2O
    CHEBI_31795["CHEBI:31795"]
    ing4_MgSO4_x_7_H2O -.-> CHEBI_31795
    ing5_Agar["Agar"]
    CultureMech_000771 --> ing5_Agar
    CHEBI_2509["CHEBI:2509"]
    ing5_Agar -.-> CHEBI_2509
    ing6_NaCl["NaCl"]
    CultureMech_000771 --> ing6_NaCl
    CHEBI_26710["CHEBI:26710"]
    ing6_NaCl -.-> CHEBI_26710
    ing7_Na2CO3["Na2CO3"]
    CultureMech_000771 --> ing7_Na2CO3
    CHEBI_29377["CHEBI:29377"]
    ing7_Na2CO3 -.-> CHEBI_29377

Ingredients (8)

# Ingredient Concentration Ontology Notes
1 Peptone 5 G_PER_L
2 Yeast extract 5 G_PER_L
3 Glucose
C6H12O6 180.156 g/mol
10 G_PER_L CHEBI:17234
glucose
4 K2HPO4
HO4P.2K 174.174 g/mol
1 G_PER_L CHEBI:131527
dipotassium hydrogen phosphate
5 MgSO4 x 7 H2O
7H2O.Mg.O4S 246.473 g/mol
0.2 G_PER_L CHEBI:31795
magnesium sulfate heptahydrate
6 Agar 20 G_PER_L CHEBI:2509
agar
7 NaCl
Cl.Na 58.443 g/mol
80 G_PER_L CHEBI:26710
sodium chloride
8 Na2CO3
CO3.2Na 105.988 g/mol
20 G_PER_L CHEBI:29377
sodium carbonate

Preparation

  1. The NaCl and Na2CO3 were dissolved and autoclaved separately and added to the organic components at 60°C before pouring the agar medium.

Target organisms

No target organisms are recorded.

Provenance

TimestampCuratorActionNotes
2026-01-26T17:53:14.063405Z mediadive-import Imported from MediaDive Source: DSMZ, ID: 1309
2026-03-13T06:18:58.243253Z culturemech-id-assigner-v1.0 Assigned CultureMech ID Assigned stable identifier: CultureMech:000771
2026-03-17T06:30:41.000000Z mediaingredientmech-enrichment-v1.0 Added MediaIngredientMech links Linked ingredients to MediaIngredientMech identifiers
2026-03-17T19:45:09.625082+00:00 normalize_media_names_to_snake_case.py NAME_NORMALIZED Normalized name from "BACILLUS LOCISALIS MEDIUM" to "bacillus_locisalis_medium" for consistent matching
2026-06-05T07:40:50.270366+00:00 mim-legacy-to-chebi-migration-v1.0 Refreshed legacy MediaIngredientMech links to CHEBI keying Replaced 6 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme.
2026-06-10T07:46:53.716478+00:00 primary-term-label-canonicalization-v1.0 Canonicalized primary term label to OBO canonical (OAK) 2 primary term(s); ingredient name resolved by OAK sqlite:obo:chebi.
2026-10-07T13:32:40.086487+00:00 codex CURATED_MEDIUM_CONCENTRATION_SOURCE_EVIDENCE Exact medium/source membership, ingredient inventory/forms, physical-state conditions, literal snippets, and arithmetic checked. Stock boundaries, source-only rows, preparation context, and variants remain explicit follow-up tasks; secondary chemical mappings unchanged.