freshwater_hd_medium

CultureMech:000774 bacterial COMPLEX SOLID_AGAR
Original name: FRESHWATER HD MEDIUM

Overview

External ontology
mediadive.medium:1312 (source link unavailable) — FRESHWATER HD MEDIUM
Applications
  • Microbial cultivation
pH
7.2
Notes
Source: DSMZ | Link: https://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium1312.pdf

Composition

flowchart LR
    CultureMech_000774["freshwater_hd_medium"]
    ing0_CaCO3["CaCO3"]
    CultureMech_000774 --> ing0_CaCO3
    CHEBI_3311["CHEBI:3311"]
    ing0_CaCO3 -.-> CHEBI_3311
    ing1_NaHCO3["NaHCO3"]
    CultureMech_000774 --> ing1_NaHCO3
    CHEBI_32139["CHEBI:32139"]
    ing1_NaHCO3 -.-> CHEBI_32139
    ing2_Peptone["Peptone"]
    CultureMech_000774 --> ing2_Peptone
    ing3_D_Glucose["D-Glucose"]
    CultureMech_000774 --> ing3_D_Glucose
    CHEBI_17634["CHEBI:17634"]
    ing3_D_Glucose -.-> CHEBI_17634
    ing4_Yeast_extract["Yeast extract"]
    CultureMech_000774 --> ing4_Yeast_extract
    ing5_HEPES["HEPES"]
    CultureMech_000774 --> ing5_HEPES
    CHEBI_46756["CHEBI:46756"]
    ing5_HEPES -.-> CHEBI_46756
    ing6_Agar["Agar"]
    CultureMech_000774 --> ing6_Agar
    CHEBI_2509["CHEBI:2509"]
    ing6_Agar -.-> CHEBI_2509
    ing7_NH4Cl["NH4Cl"]
    CultureMech_000774 --> ing7_NH4Cl
    CHEBI_31206["CHEBI:31206"]
    ing7_NH4Cl -.-> CHEBI_31206
    ing8_KH2PO4["KH2PO4"]
    CultureMech_000774 --> ing8_KH2PO4
    CHEBI_63036["CHEBI:63036"]
    ing8_KH2PO4 -.-> CHEBI_63036
    ing9_KNO3["KNO3"]
    CultureMech_000774 --> ing9_KNO3
    CHEBI_63043["CHEBI:63043"]
    ing9_KNO3 -.-> CHEBI_63043
    ing10_MgSO4_x_7_H2O["MgSO4 x 7 H2O"]
    CultureMech_000774 --> ing10_MgSO4_x_7_H2O
    CHEBI_31795["CHEBI:31795"]
    ing10_MgSO4_x_7_H2O -.-> CHEBI_31795
    ing11_CaCl2_x_2_H2O["CaCl2 x 2 H2O"]
    CultureMech_000774 --> ing11_CaCl2_x_2_H2O
    CHEBI_86158["CHEBI:86158"]
    ing11_CaCl2_x_2_H2O -.-> CHEBI_86158

Ingredients (12)

# Ingredient Concentration Ontology Notes
1 CaCO3
CO3.Ca 100.086 g/mol
0.025 G_PER_L CHEBI:3311
calcium carbonate
2 NaHCO3
CHO3.Na 84.006 g/mol
0.025 G_PER_L CHEBI:32139
sodium hydrogencarbonate
3 Peptone 0.5 G_PER_L
4 D-Glucose
C6H12O6 180.156 g/mol
0.1 G_PER_L CHEBI:17634
D-glucose
5 Yeast extract 0.25 G_PER_L
6 HEPES 2.38 G_PER_L CHEBI:46756
HEPES
7 Agar 14 G_PER_L CHEBI:2509
agar
 (if appropriate)
8 NH4Cl
Cl.H4N 53.492 g/mol
0.0053 G_PER_L CHEBI:31206
ammonium chloride
9 KH2PO4
H2O4P.K 136.084 g/mol
0.014 G_PER_L CHEBI:63036
potassium dihydrogen phosphate
10 KNO3
K.NO3 101.102 g/mol
0.1 G_PER_L CHEBI:63043
potassium nitrate
11 MgSO4 x 7 H2O
7H2O.Mg.O4S 246.473 g/mol
0.493 G_PER_L CHEBI:31795
magnesium sulfate heptahydrate
12 CaCl2 x 2 H2O
Ca.2Cl.2H2O 147.014 g/mol
0.147 G_PER_L CHEBI:86158
calcium chloride dihydrate

Preparation

  1. Dissolve carbonates in 900 ml distilled water by stirring for 5 h or longer. Add other components while stirring, adjust pH to 7.2 with KOH. Autoclave.
  2. Autoclave solution A and B separately and combine after cooling.

Target organisms

No target organisms are recorded.

Provenance

TimestampCuratorActionNotes
2026-01-26T17:53:14.069915Z mediadive-import Imported from MediaDive Source: DSMZ, ID: 1312
2026-03-13T06:18:58.261637Z culturemech-id-assigner-v1.0 Assigned CultureMech ID Assigned stable identifier: CultureMech:000774
2026-03-17T06:30:45.000000Z mediaingredientmech-enrichment-v1.0 Added MediaIngredientMech links Linked ingredients to MediaIngredientMech identifiers
2026-03-17T19:45:09.650253+00:00 normalize_media_names_to_snake_case.py NAME_NORMALIZED Normalized name from "FRESHWATER HD MEDIUM" to "freshwater_hd_medium" for consistent matching
2026-06-05T07:41:05.440699+00:00 mim-legacy-to-chebi-migration-v1.0 Refreshed legacy MediaIngredientMech links to CHEBI keying Replaced 9 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme.
2026-06-10T07:37:34.886411+00:00 primary-term-regrounding-v1.0 Re-grounded wrong primary term id (OAK-verified) 1 primary term(s); ingredient name resolved by OAK sqlite:obo:chebi.
2026-06-10T07:46:58.790709+00:00 primary-term-label-canonicalization-v1.0 Canonicalized primary term label to OBO canonical (OAK) 3 primary term(s); ingredient name resolved by OAK sqlite:obo:chebi.