methanocella_conradii_medium

CultureMech:000776 archaea COMPLEX LIQUID
Original name: METHANOCELLA CONRADII MEDIUM

Overview

External ontology
mediadive.medium:1318 (source link unavailable) — METHANOCELLA CONRADII MEDIUM
Applications
  • Microbial cultivation
pH
7.0
Notes
Source: DSMZ | Link: https://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium1318.pdf Composition verified against https://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium1318.pdf on 2026-08-25 (SHA-256 f2ed1e926a6f1e63b27c3a3690b0d42a871220ee01e43d3ef5a85643fd1d3834). Direct quantities and stock additions were scaled from the printed 1004 ml batch to a one-litre final basis.

Composition

flowchart LR
    CultureMech_000776["methanocella_conradii_medium"]
    ing0_MgCl2_x_6_H2O["MgCl2 x 6 H2O"]
    CultureMech_000776 --> ing0_MgCl2_x_6_H2O
    CHEBI_86345["CHEBI:86345"]
    ing0_MgCl2_x_6_H2O -.-> CHEBI_86345
    ing1_CaCl2_x_2_H2O["CaCl2 x 2 H2O"]
    CultureMech_000776 --> ing1_CaCl2_x_2_H2O
    CHEBI_86158["CHEBI:86158"]
    ing1_CaCl2_x_2_H2O -.-> CHEBI_86158
    ing2_NH4Cl["NH4Cl"]
    CultureMech_000776 --> ing2_NH4Cl
    CHEBI_31206["CHEBI:31206"]
    ing2_NH4Cl -.-> CHEBI_31206
    ing3_KH2PO4["KH2PO4"]
    CultureMech_000776 --> ing3_KH2PO4
    CHEBI_63036["CHEBI:63036"]
    ing3_KH2PO4 -.-> CHEBI_63036
    ing4_KCl["KCl"]
    CultureMech_000776 --> ing4_KCl
    CHEBI_32588["CHEBI:32588"]
    ing4_KCl -.-> CHEBI_32588
    ing5_Yeast_extract["Yeast extract"]
    CultureMech_000776 --> ing5_Yeast_extract
    ing6_Na_acetate["Na-acetate"]
    CultureMech_000776 --> ing6_Na_acetate
    CHEBI_32954["CHEBI:32954"]
    ing6_Na_acetate -.-> CHEBI_32954
    ing7_Na2CO3["Na2CO3"]
    CultureMech_000776 --> ing7_Na2CO3
    CHEBI_29377["CHEBI:29377"]
    ing7_Na2CO3 -.-> CHEBI_29377
    ing8_L_Cysteine_HCl_x_H2O["L-Cysteine HCl x H2O"]
    CultureMech_000776 --> ing8_L_Cysteine_HCl_x_H2O
    CHEBI_91248["CHEBI:91248"]
    ing8_L_Cysteine_HCl_x_H2O -.-> CHEBI_91248
    ing9_DL_Dithiothreitol["DL-Dithiothreitol"]
    CultureMech_000776 --> ing9_DL_Dithiothreitol
    CHEBI_18320["CHEBI:18320"]
    ing9_DL_Dithiothreitol -.-> CHEBI_18320
    ing10_Distilled_water["Distilled water"]
    CultureMech_000776 --> ing10_Distilled_water
    CHEBI_15377["CHEBI:15377"]
    ing10_Distilled_water -.-> CHEBI_15377

Ingredients (11)

# Ingredient Concentration Ontology Notes
1 MgCl2 x 6 H2O
2Cl.6H2O.Mg 203.301 g/mol
0.398406 G_PER_L CHEBI:86345
magnesium dichloride hexahydrate
2 CaCl2 x 2 H2O
Ca.2Cl.2H2O 147.014 g/mol
0.099602 G_PER_L CHEBI:86158
calcium chloride dihydrate
3 NH4Cl
Cl.H4N 53.492 g/mol
0.099602 G_PER_L CHEBI:31206
ammonium chloride
4 KH2PO4
H2O4P.K 136.084 g/mol
0.199203 G_PER_L CHEBI:63036
potassium dihydrogen phosphate
5 KCl
Cl.K 74.551 g/mol
0.498008 G_PER_L CHEBI:32588
potassium chloride
6 Yeast extract 0.199203 G_PER_L FOODON:03315426
yeast extract
7 Na-acetate
C2H3O2.Na 82.034 g/mol
0.089641 G_PER_L CHEBI:32954
sodium acetate
8 Na2CO3
CO3.2Na 105.988 g/mol
0.996016 G_PER_L CHEBI:29377
sodium carbonate
 The source requires addition from a sterile anoxic stock under 80% N2 and 20% CO2 but does not specify that stock's strength or volume.
9 L-Cysteine HCl x H2O
C3H7NO2S.H2O.HCl 175.637 g/mol
0.298805 G_PER_L CHEBI:91248
L-cysteine hydrochloride hydrate
 The source requires addition from a sterile anoxic stock under 100% N2 but does not specify that stock's strength or volume.
10 DL-Dithiothreitol
C4H10O2S2 154.256 g/mol
0.298805 G_PER_L CHEBI:18320
1,4-dithiothreitol
 The source requires addition from a sterile anoxic stock under 100% N2 but does not specify that stock's strength or volume.
11 Distilled water
H2O 18.015 g/mol
996.015936 ML_PER_L CHEBI:15377
water
 The source prints 1000 ml water in a 1004 ml final batch.

Preparation

  1. Dissolve all ingredients except carbonate, vitamins, cysteine, and DTT. Include SL-10, selenite-tungstate, and resazurin stocks.
  2. Sparge with 80% N2 and 20% CO2 for 30-45 minutes, dispense under that atmosphere to 30% of vessel volume, and autoclave.
  3. Add filter-sterilized vitamin stocks and sterile anoxic cysteine and DTT stocks prepared under 100% N2. Add carbonate from a sterile anoxic stock prepared under 80% N2 and 20% CO2.
  4. The complete medium should have pH 7.0.
  5. After inoculation, pressurize vials to 1 bar overpressure with sterile 100% H2 gas.
  6. For SL-10, first dissolve FeCl2 in 25% HCl, dilute in water, add the other salts, and make to 1 litre.

Solutions

Trace element solution SL-10

Dose: 0.996016 ML_PER_L

Stock composition
IngredientConcentrationNotes
HCl (25%) 10 ML_PER_L
FeCl2 x 4 H2O 1.50 G_PER_L
ZnCl2 0.070 G_PER_L
MnCl2 x 4 H2O 0.100 G_PER_L
H3BO3 0.006 G_PER_L
CoCl2 x 6 H2O 0.190 G_PER_L MIM maps the hydrated source label to cobalt dichloride.
CuCl2 x 2 H2O 0.002 G_PER_L
NiCl2 x 6 H2O 0.024 G_PER_L MIM maps the hydrated source label to nickel dichloride.
Na2MoO4 x 2 H2O 0.036 G_PER_L
Distilled water 990 ML_PER_L
Add 1 ml in the printed 1004 ml batch. Formula is from DSMZ medium 320 as reproduced in the reviewed DSMZ 1318 PDF.

Selenite-tungstate solution

Dose: 0.996016 ML_PER_L

Stock composition
IngredientConcentrationNotes
NaOH 0.50 G_PER_L
Na2SeO3 x 5 H2O 0.003 G_PER_L
Na2WO4 x 2 H2O 0.004 G_PER_L
Distilled water 1000 ML_PER_L
Add 1 ml in the printed 1004 ml batch. Formula is from DSMZ medium 385 as reproduced in the reviewed PDF.

Sodium resazurin solution (0.1% w/v)

Dose: 0.498008 ML_PER_L

Stock composition
IngredientConcentrationNotes
Resazurin 1.0 G_PER_L
Distilled water 1000 ML_PER_L
Add 0.5 ml in the printed 1004 ml batch.

Wolin's vitamin solution (10x)

Dose: 0.996016 ML_PER_L

Stock composition
IngredientConcentrationNotes
Biotin 0.020 G_PER_L
Folic acid 0.020 G_PER_L
Pyridoxine hydrochloride 0.100 G_PER_L
Thiamine HCl 0.050 G_PER_L
Riboflavin 0.050 G_PER_L
Nicotinic acid 0.050 G_PER_L
Calcium D-(+)-pantothenate 0.050 G_PER_L
Vitamin B12 0.001 G_PER_L
p-Aminobenzoic acid 0.050 G_PER_L
(DL)-alpha-Lipoic acid 0.050 G_PER_L
Distilled water 1000 ML_PER_L
Add 1 ml in the printed 1004 ml batch. Formula is from DSMZ medium 120 as reproduced in the reviewed PDF.

Seven vitamins solution

Dose: 0.996016 ML_PER_L

Stock composition
IngredientConcentrationNotes
Vitamin B12 0.100 G_PER_L
p-Aminobenzoic acid 0.080 G_PER_L
D-(+)-biotin 0.020 G_PER_L
Nicotinic acid 0.200 G_PER_L
Calcium pantothenate 0.100 G_PER_L
Pyridoxine hydrochloride 0.300 G_PER_L
Thiamine-HCl x 2 H2O 0.200 G_PER_L
Distilled water 1000 ML_PER_L
Add 1 ml in the printed 1004 ml batch. Formula is from DSMZ medium 503 as reproduced in the reviewed PDF.

Target organisms

No target organisms are recorded.

Provenance

TimestampCuratorActionNotes
2026-01-26T17:53:14.084566Z mediadive-import Imported from MediaDive Source: DSMZ, ID: 1318
2026-02-06T05:10:55.885337Z invalid-chebi-removal Removed 1 invalid CHEBI ID(s) Removed CHEBI IDs with invalid format (7+ digits) from MicrobeMediaParam source data
2026-03-13T03:09:45.014100Z data-quality-cleanup-v1.0 Data quality fixes Merged 4 duplicate ingredient(s)
2026-03-13T06:18:58.277456Z culturemech-id-assigner-v1.0 Assigned CultureMech ID Assigned stable identifier: CultureMech:000776
2026-03-17T06:30:58.000000Z mediaingredientmech-enrichment-v1.0 Added MediaIngredientMech links Linked ingredients to MediaIngredientMech identifiers
2026-03-17T19:45:09.667344+00:00 normalize_media_names_to_snake_case.py NAME_NORMALIZED Normalized name from "METHANOCELLA CONRADII MEDIUM" to "methanocella_conradii_medium" for consistent matching
2026-06-05T07:41:31.123867+00:00 mim-legacy-to-chebi-migration-v1.0 Refreshed legacy MediaIngredientMech links to CHEBI keying Replaced 33 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme.
2026-06-10T07:47:06.820442+00:00 primary-term-label-canonicalization-v1.0 Canonicalized primary term label to OBO canonical (OAK) 5 primary term(s); ingredient name resolved by OAK sqlite:obo:chebi.
2026-08-07T23:42:25.187707+00:00 apply_cocktail_nesting.py NESTED_FLATTENED_COCKTAIL Moved 6 stock-strength component(s) out of ingredients into 3 solution(s): 1 -> 'Trace element solution SL-10' @ 1 ml/l; 1 -> 'Seven vitamins solution' @ 1 ml/l; 0 -> "Wolin's vitamin solution (10x)" @ 1 ml/l. 4 component(s) were SUMMED across stocks by the flattening and are restored to each stock's own value, verified by exact sum: nicotinic acid, p-aminobenzoic acid, pyridoxine hydrochloride, vitamin b12. Each moved component matched the MediaDive stock by name AND value; bulk ingredients sharing a name were left in place (#150).
2026-08-25T00:00:00-07:00 repair_dsmz_1318_family.py CORRECTED_DSMZ_1318_SOLUTION_STRUCTURE Corrected final-volume scaling, restored trace and selenite stocks, separated Wolin and seven-vitamin stocks, and removed gas atmospheres from ingredients. Identity objects were verified against MIM.