half_strength_r2a_medium_in_75_seawater

Overview

External ontology

mediadive.medium:1320 (source link unavailable) — HALF STRENGTH R2A MEDIUM IN 75% SEAWATER

Applications

• Microbial cultivation

Notes

Source: DSMZ | Link: https://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium1320.pdf

Composition

flowchart LR
    CultureMech_000777["half_strength_r2a_medium_in_75_seawater"]
    ing0_Yeast_extract["Yeast extract"]
    CultureMech_000777 --> ing0_Yeast_extract
    ing1_Proteose_peptone["Proteose peptone"]
    CultureMech_000777 --> ing1_Proteose_peptone
    ing2_Casamino_acids["Casamino acids"]
    CultureMech_000777 --> ing2_Casamino_acids
    ing3_Glucose["Glucose"]
    CultureMech_000777 --> ing3_Glucose
    CHEBI_17234["CHEBI:17234"]
    ing3_Glucose -.-> CHEBI_17234
    ing4_Starch["Starch"]
    CultureMech_000777 --> ing4_Starch
    CHEBI_28017["CHEBI:28017"]
    ing4_Starch -.-> CHEBI_28017
    ing5_Na_pyruvate["Na-pyruvate"]
    CultureMech_000777 --> ing5_Na_pyruvate
    CHEBI_50144["CHEBI:50144"]
    ing5_Na_pyruvate -.-> CHEBI_50144
    ing6_K2HPO4["K2HPO4"]
    CultureMech_000777 --> ing6_K2HPO4
    CHEBI_131527["CHEBI:131527"]
    ing6_K2HPO4 -.-> CHEBI_131527
    ing7_MgSO4_x_7_H2O["MgSO4 x 7 H2O"]
    CultureMech_000777 --> ing7_MgSO4_x_7_H2O
    CHEBI_31795["CHEBI:31795"]
    ing7_MgSO4_x_7_H2O -.-> CHEBI_31795
    ing8_Agar["Agar"]
    CultureMech_000777 --> ing8_Agar
    CHEBI_2509["CHEBI:2509"]
    ing8_Agar -.-> CHEBI_2509

Ingredients (9)

Preparation

1. Refer to the composition of DSMZ medium 830 and either make up the medium in 2 litres of 75% seawater or use half of the amounts of the components specified and make up the medium in 1 litre of 75% seawater. Liquid medium can be prepared by leaving out the agar.
2. Final pH 7.2; adjust with crystalline K2HPO4 or KH2PO4 before adding agar. Add agar, heat medium to boiling to dissolve agar, and autoclave for 15 min at 121°C.

Target organisms

No target organisms are recorded.

Provenance