Overview
- External ontology
- mediadive.medium:1321 (source link unavailable) — ACIDIHALOBACTER FERROOXIDANS MEDIUM
- Applications
-
- Microbial cultivation
- Notes
- Source: DSMZ | Link: https://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium1321.pdf
Composition
flowchart LR
CultureMech_000778["acidihalobacter_ferrooxidans_medium"]
ing0_NaCl["NaCl"]
CultureMech_000778 --> ing0_NaCl
CHEBI_26710["CHEBI:26710"]
ing0_NaCl -.-> CHEBI_26710
ing1__NH4_2SO4["(NH4)2SO4"]
CultureMech_000778 --> ing1__NH4_2SO4
CHEBI_62946["CHEBI:62946"]
ing1__NH4_2SO4 -.-> CHEBI_62946
ing2_MgSO4_x_7_H2O["MgSO4 x 7 H2O"]
CultureMech_000778 --> ing2_MgSO4_x_7_H2O
CHEBI_31795["CHEBI:31795"]
ing2_MgSO4_x_7_H2O -.-> CHEBI_31795
ing3_K2HPO4["K2HPO4"]
CultureMech_000778 --> ing3_K2HPO4
CHEBI_131527["CHEBI:131527"]
ing3_K2HPO4 -.-> CHEBI_131527
ing4_FeSO4_x_7_H2O["FeSO4 x 7 H2O"]
CultureMech_000778 --> ing4_FeSO4_x_7_H2O
CHEBI_75836["CHEBI:75836"]
ing4_FeSO4_x_7_H2O -.-> CHEBI_75836
ing5_K2S4O6["K2S4O6"]
CultureMech_000778 --> ing5_K2S4O6
CHEBI_86466["CHEBI:86466"]
ing5_K2S4O6 -.-> CHEBI_86466
ing6_Yeast_extract["Yeast extract"]
CultureMech_000778 --> ing6_Yeast_extract
Ingredients (7)
| # | Ingredient | Concentration | Ontology | Notes |
|---|---|---|---|---|
| 1 |
NaCl
Cl.Na 58.443 g/mol
|
16.6667 G_PER_L |
CHEBI:26710
sodium chloride
|
|
| 2 |
(NH4)2SO4
2H4N.O4S 132.141 g/mol
|
0.222222 G_PER_L |
CHEBI:62946
ammonium sulfate
|
|
| 3 |
MgSO4 x 7 H2O
7H2O.Mg.O4S 246.473 g/mol
|
0.444444 G_PER_L |
CHEBI:31795
magnesium sulfate heptahydrate
|
|
| 4 |
K2HPO4
HO4P.2K 174.174 g/mol
|
0.111111 G_PER_L |
CHEBI:131527
dipotassium hydrogen phosphate
|
|
| 5 |
FeSO4 x 7 H2O
Fe.7H2O.O4S 278.013 g/mol
|
139 G_PER_L |
CHEBI:75836
iron(2+) sulfate heptahydrate
|
|
| 6 |
K2S4O6
2K.O6S4 302.458 g/mol
|
30 G_PER_L |
CHEBI:86466
potassium tetrathionate
|
|
| 7 | Yeast extract | 10 G_PER_L |
Preparation
- Combine solution A and solution B after cooling and add solutions C and D to complete the medium.
- Note: Use 10% (v/v) as inoculum.
- Adjust the pH to 1.7 with H2SO4 and autoclave at 121°C for 20 min.
- Adjust the pH to 1.25 and autoclave solution at 115°C for 10 min or sterilize by filtration.
- Sterilize by filtration.
- Autoclave at 121°C for 20 min.
Target organisms
No target organisms are recorded.
Provenance
| Timestamp | Curator | Action | Notes |
|---|---|---|---|
| 2026-01-26T17:53:14.092035Z | mediadive-import | Imported from MediaDive | Source: DSMZ, ID: 1321 |
| 2026-01-28T19:48:30.528278Z | chebi-enrichment | Added CHEBI ontology terms to ingredients | Enriched using MicrobeMediaParam and MediaDive chemical mappings |
| 2026-03-13T06:18:58.292491Z | culturemech-id-assigner-v1.0 | Assigned CultureMech ID | Assigned stable identifier: CultureMech:000778 |
| 2026-03-17T06:30:30.000000Z | mediaingredientmech-enrichment-v1.0 | Added MediaIngredientMech links | Linked ingredients to MediaIngredientMech identifiers |
| 2026-03-17T19:45:09.683857+00:00 | normalize_media_names_to_snake_case.py | NAME_NORMALIZED | Normalized name from "ACIDIHALOBACTER FERROOXIDANS MEDIUM" to "acidihalobacter_ferrooxidans_medium" for consistent matching |
| 2026-06-05T07:40:47.226882+00:00 | mim-legacy-to-chebi-migration-v1.0 | Refreshed legacy MediaIngredientMech links to CHEBI keying | Replaced 6 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme. |
| 2026-06-10T07:46:52.566908+00:00 | primary-term-label-canonicalization-v1.0 | Canonicalized primary term label to OBO canonical (OAK) | 2 primary term(s); ingredient name resolved by OAK sqlite:obo:chebi. |