Overview
- External ontology
- mediadive.medium:1323 (source link unavailable) — METHYLOCAPSA AUREA MEDIUM
- Applications
-
- Microbial cultivation
- pH range
- {'min': 5.0, 'max': 5.8}
- Notes
- Source: DSMZ | Link: https://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium1323.pdf
Composition
flowchart LR
CultureMech_000780["methylocapsa_aurea_medium"]
ing0_NaNO3["NaNO3"]
CultureMech_000780 --> ing0_NaNO3
CHEBI_63005["CHEBI:63005"]
ing0_NaNO3 -.-> CHEBI_63005
ing1_MgSO4_x_6_H2O["MgSO4 x 6 H2O"]
CultureMech_000780 --> ing1_MgSO4_x_6_H2O
CHEBI_32599["CHEBI:32599"]
ing1_MgSO4_x_6_H2O -.-> CHEBI_32599
ing2_CaCl2_x_2_H2O["CaCl2 x 2 H2O"]
CultureMech_000780 --> ing2_CaCl2_x_2_H2O
CHEBI_86158["CHEBI:86158"]
ing2_CaCl2_x_2_H2O -.-> CHEBI_86158
ing3_EDTA["EDTA"]
CultureMech_000780 --> ing3_EDTA
CHEBI_4735["CHEBI:4735"]
ing3_EDTA -.-> CHEBI_4735
ing4_CuCl2_x_5_H2O["CuCl2 x 5 H2O"]
CultureMech_000780 --> ing4_CuCl2_x_5_H2O
CHEBI_91245["CHEBI:91245"]
ing4_CuCl2_x_5_H2O -.-> CHEBI_91245
ing5_FeSO4_x_7_H2O["FeSO4 x 7 H2O"]
CultureMech_000780 --> ing5_FeSO4_x_7_H2O
CHEBI_75836["CHEBI:75836"]
ing5_FeSO4_x_7_H2O -.-> CHEBI_75836
ing6_ZnSO4_x_7_H2O["ZnSO4 x 7 H2O"]
CultureMech_000780 --> ing6_ZnSO4_x_7_H2O
CHEBI_32312["CHEBI:32312"]
ing6_ZnSO4_x_7_H2O -.-> CHEBI_32312
ing7_NiCl2_x_6_H2O["NiCl2 x 6 H2O"]
CultureMech_000780 --> ing7_NiCl2_x_6_H2O
CHEBI_34887["CHEBI:34887"]
ing7_NiCl2_x_6_H2O -.-> CHEBI_34887
ing8_CoCl2_x_6_H2O["CoCl2 x 6 H2O"]
CultureMech_000780 --> ing8_CoCl2_x_6_H2O
CHEBI_53503["CHEBI:53503"]
ing8_CoCl2_x_6_H2O -.-> CHEBI_53503
ing9_Na2MoO4["Na2MoO4"]
CultureMech_000780 --> ing9_Na2MoO4
CHEBI_75215["CHEBI:75215"]
ing9_Na2MoO4 -.-> CHEBI_75215
ing10_Na2_EDTA["Na2-EDTA"]
CultureMech_000780 --> ing10_Na2_EDTA
CHEBI_64734["CHEBI:64734"]
ing10_Na2_EDTA -.-> CHEBI_64734
ing11_K2HPO4_x_3_H2O["K2HPO4 x 3 H2O"]
CultureMech_000780 --> ing11_K2HPO4_x_3_H2O
CHEBI_131527["CHEBI:131527"]
ing11_K2HPO4_x_3_H2O -.-> CHEBI_131527
ing12_KH2PO4["KH2PO4"]
CultureMech_000780 --> ing12_KH2PO4
CHEBI_63036["CHEBI:63036"]
ing12_KH2PO4 -.-> CHEBI_63036
Ingredients (13)
| # | Ingredient | Concentration | Ontology | Notes |
|---|---|---|---|---|
| 1 |
NaNO3
NO3.Na 84.994 g/mol
|
0.2 G_PER_L |
CHEBI:63005
sodium nitrate
|
|
| 2 |
MgSO4 x 6 H2O
Mg.O4S 120.368 g/mol
|
0.2 G_PER_L |
CHEBI:32599
magnesium sulfate
|
|
| 3 |
CaCl2 x 2 H2O
Ca.2Cl.2H2O 147.014 g/mol
|
0.04 G_PER_L |
CHEBI:86158
calcium chloride dihydrate
|
|
| 4 |
EDTA
C10H16N2O8 292.244 g/mol
|
5 G_PER_L |
CHEBI:4735
ethylenediaminetetraacetic acid
|
|
| 5 |
CuCl2 x 5 H2O
Cl2Cu.5H2O 224.527 g/mol
|
0.1 G_PER_L |
CHEBI:91245
copper(II) chloride pentahydrate
|
|
| 6 |
FeSO4 x 7 H2O
Fe.7H2O.O4S 278.013 g/mol
|
3.54 G_PER_L |
CHEBI:75836
iron(2+) sulfate heptahydrate
|
[Merged 2 duplicates: 2.0, 1.54] |
| 7 |
ZnSO4 x 7 H2O
7H2O.O4S.Zn 287.558 g/mol
|
0.1 G_PER_L |
CHEBI:32312
zinc sulfate heptahydrate
|
|
| 8 |
NiCl2 x 6 H2O
Cl2Ni 129.599 g/mol
|
0.02 G_PER_L |
CHEBI:34887
nickel dichloride
|
|
| 9 |
CoCl2 x 6 H2O
2Cl.Co.6H2O 237.929 g/mol
|
0.2 G_PER_L |
CHEBI:53503
cobalt chloride hexahydrate
|
|
| 10 |
Na2MoO4
MoO4.2Na 205.916 g/mol
|
0.03 G_PER_L |
CHEBI:75215
sodium molybdate (anhydrous)
|
|
| 11 |
Na2-EDTA
C10H14N2O8.2Na 336.208 g/mol
|
2.06 G_PER_L |
CHEBI:64734
EDTA disodium salt (anhydrous)
|
|
| 12 |
K2HPO4 x 3 H2O
HO4P.2K 174.174 g/mol
|
1.71 G_PER_L |
CHEBI:131527
dipotassium hydrogen phosphate
|
|
| 13 |
KH2PO4
H2O4P.K 136.084 g/mol
|
12.58 G_PER_L |
CHEBI:63036
potassium dihydrogen phosphate
|
Preparation
- Final pH 5.0-5.8.
- 20-30% methane is added to the gas phase. The strain should be grown with shaking.
- pH 5.8
Target organisms
No target organisms are recorded.
Provenance
| Timestamp | Curator | Action | Notes |
|---|---|---|---|
| 2026-01-26T17:53:14.097189Z | mediadive-import | Imported from MediaDive | Source: DSMZ, ID: 1323 |
| 2026-03-13T03:08:46.065579Z | data-quality-cleanup-v1.0 | Data quality fixes | Merged 1 duplicate ingredient(s) |
| 2026-03-13T06:18:58.307166Z | culturemech-id-assigner-v1.0 | Assigned CultureMech ID | Assigned stable identifier: CultureMech:000780 |
| 2026-03-17T06:29:49.000000Z | mediaingredientmech-enrichment-v1.0 | Added MediaIngredientMech links | Linked ingredients to MediaIngredientMech identifiers |
| 2026-03-17T19:45:09.700315+00:00 | normalize_media_names_to_snake_case.py | NAME_NORMALIZED | Normalized name from "METHYLOCAPSA AUREA MEDIUM" to "methylocapsa_aurea_medium" for consistent matching |
| 2026-05-20T06:01:56.734654+00:00 | migrate_ph_range.py | MIGRATED_PH_RANGE | ph_range '5-5.8' -> {'min': 5.0, 'max': 5.8} |
| 2026-06-05T07:41:33.056996+00:00 | mim-legacy-to-chebi-migration-v1.0 | Refreshed legacy MediaIngredientMech links to CHEBI keying | Replaced 12 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme. |
| 2026-06-10T07:47:07.531247+00:00 | primary-term-label-canonicalization-v1.0 | Canonicalized primary term label to OBO canonical (OAK) | 3 primary term(s); ingredient name resolved by OAK sqlite:obo:chebi. |