defluviitoga_medium

CultureMech:000785 bacterial COMPLEX LIQUID
Original name: DEFLUVIITOGA MEDIUM

Overview

External ontology
mediadive.medium:1328 (source link unavailable) — DEFLUVIITOGA MEDIUM
Applications
  • Microbial cultivation
pH
7.0
Notes
Source: DSMZ | Link: https://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium1328.pdf

Composition

flowchart LR
    CultureMech_000785["defluviitoga_medium"]
    ing0_KH2PO4["KH2PO4"]
    CultureMech_000785 --> ing0_KH2PO4
    CHEBI_63036["CHEBI:63036"]
    ing0_KH2PO4 -.-> CHEBI_63036
    ing1_K2HPO4["K2HPO4"]
    CultureMech_000785 --> ing1_K2HPO4
    CHEBI_131527["CHEBI:131527"]
    ing1_K2HPO4 -.-> CHEBI_131527
    ing2_NH4Cl["NH4Cl"]
    CultureMech_000785 --> ing2_NH4Cl
    CHEBI_31206["CHEBI:31206"]
    ing2_NH4Cl -.-> CHEBI_31206
    ing3_NaCl["NaCl"]
    CultureMech_000785 --> ing3_NaCl
    CHEBI_26710["CHEBI:26710"]
    ing3_NaCl -.-> CHEBI_26710
    ing4_KCl["KCl"]
    CultureMech_000785 --> ing4_KCl
    CHEBI_32588["CHEBI:32588"]
    ing4_KCl -.-> CHEBI_32588
    ing5_MgCl2_x_6_H2O["MgCl2 x 6 H2O"]
    CultureMech_000785 --> ing5_MgCl2_x_6_H2O
    CHEBI_86345["CHEBI:86345"]
    ing5_MgCl2_x_6_H2O -.-> CHEBI_86345
    ing6_CaCl2_x_2_H2O["CaCl2 x 2 H2O"]
    CultureMech_000785 --> ing6_CaCl2_x_2_H2O
    CHEBI_86158["CHEBI:86158"]
    ing6_CaCl2_x_2_H2O -.-> CHEBI_86158
    ing7_Yeast_extract["Yeast extract"]
    CultureMech_000785 --> ing7_Yeast_extract
    ing8_Sodium_resazurin["Sodium resazurin"]
    CultureMech_000785 --> ing8_Sodium_resazurin
    CHEBI_8806["CHEBI:8806"]
    ing8_Sodium_resazurin -.-> CHEBI_8806
    ing9_Sulfur["Sulfur"]
    CultureMech_000785 --> ing9_Sulfur
    CHEBI_26833["CHEBI:26833"]
    ing9_Sulfur -.-> CHEBI_26833
    ing10_L_Cysteine_HCl_x_H2O["L-Cysteine HCl x H2O"]
    CultureMech_000785 --> ing10_L_Cysteine_HCl_x_H2O
    CHEBI_91248["CHEBI:91248"]
    ing10_L_Cysteine_HCl_x_H2O -.-> CHEBI_91248
    ing11_Trypticase_peptone["Trypticase peptone"]
    CultureMech_000785 --> ing11_Trypticase_peptone
    ing12_Na2_fumarate["Na2-fumarate"]
    CultureMech_000785 --> ing12_Na2_fumarate
    CHEBI_115156["CHEBI:115156"]
    ing12_Na2_fumarate -.-> CHEBI_115156
    ing13_Na2CO3["Na2CO3"]
    CultureMech_000785 --> ing13_Na2CO3
    CHEBI_29377["CHEBI:29377"]
    ing13_Na2CO3 -.-> CHEBI_29377
    ing14_Na2S_x_9_H2O["Na2S x 9 H2O"]
    CultureMech_000785 --> ing14_Na2S_x_9_H2O
    CHEBI_76209["CHEBI:76209"]
    ing14_Na2S_x_9_H2O -.-> CHEBI_76209
    ing15_HCl["HCl"]
    CultureMech_000785 --> ing15_HCl
    CHEBI_17883["CHEBI:17883"]
    ing15_HCl -.-> CHEBI_17883
    ing16_FeCl2_x_4_H2O["FeCl2 x 4 H2O"]
    CultureMech_000785 --> ing16_FeCl2_x_4_H2O
    CHEBI_86249["CHEBI:86249"]
    ing16_FeCl2_x_4_H2O -.-> CHEBI_86249
    ing17_ZnCl2["ZnCl2"]
    CultureMech_000785 --> ing17_ZnCl2
    CHEBI_49976["CHEBI:49976"]
    ing17_ZnCl2 -.-> CHEBI_49976
    ing18_MnCl2_x_4_H2O["MnCl2 x 4 H2O"]
    CultureMech_000785 --> ing18_MnCl2_x_4_H2O
    CHEBI_86368["CHEBI:86368"]
    ing18_MnCl2_x_4_H2O -.-> CHEBI_86368
    ing19_H3BO3["H3BO3"]
    CultureMech_000785 --> ing19_H3BO3
    CHEBI_33118["CHEBI:33118"]
    ing19_H3BO3 -.-> CHEBI_33118
    ing20_CoCl2_x_6_H2O["CoCl2 x 6 H2O"]
    CultureMech_000785 --> ing20_CoCl2_x_6_H2O
    CHEBI_53503["CHEBI:53503"]
    ing20_CoCl2_x_6_H2O -.-> CHEBI_53503
    ing21_CuCl2_x_2_H2O["CuCl2 x 2 H2O"]
    CultureMech_000785 --> ing21_CuCl2_x_2_H2O
    CHEBI_86318["CHEBI:86318"]
    ing21_CuCl2_x_2_H2O -.-> CHEBI_86318
    ing22_NiCl2_x_6_H2O["NiCl2 x 6 H2O"]
    CultureMech_000785 --> ing22_NiCl2_x_6_H2O
    CHEBI_34887["CHEBI:34887"]
    ing22_NiCl2_x_6_H2O -.-> CHEBI_34887
    ing23_Na2MoO4_x_2_H2O["Na2MoO4 x 2 H2O"]
    CultureMech_000785 --> ing23_Na2MoO4_x_2_H2O
    CHEBI_75213["CHEBI:75213"]
    ing23_Na2MoO4_x_2_H2O -.-> CHEBI_75213

Ingredients (24)

# Ingredient Concentration Ontology Notes
1 KH2PO4
H2O4P.K 136.084 g/mol
0.3 G_PER_L CHEBI:63036
potassium dihydrogen phosphate
2 K2HPO4
HO4P.2K 174.174 g/mol
0.3 G_PER_L CHEBI:131527
dipotassium hydrogen phosphate
3 NH4Cl
Cl.H4N 53.492 g/mol
1 G_PER_L CHEBI:31206
ammonium chloride
4 NaCl
Cl.Na 58.443 g/mol
1 G_PER_L CHEBI:26710
sodium chloride
5 KCl
Cl.K 74.551 g/mol
0.1 G_PER_L CHEBI:32588
potassium chloride
6 MgCl2 x 6 H2O
2Cl.6H2O.Mg 203.301 g/mol
0.5 G_PER_L CHEBI:86345
magnesium dichloride hexahydrate
7 CaCl2 x 2 H2O
Ca.2Cl.2H2O 147.014 g/mol
0.1 G_PER_L CHEBI:86158
calcium chloride dihydrate
8 Yeast extract 1 G_PER_L
9 Sodium resazurin
C12H7NO4 229.191 g/mol
0.0005 G_PER_L CHEBI:8806
Resazurin
10 Sulfur
S 32.067 g/mol
10 G_PER_L CHEBI:26833
sulfur atom
11 L-Cysteine HCl x H2O
C3H7NO2S.H2O.HCl 175.637 g/mol
0.5 G_PER_L CHEBI:91248
L-cysteine hydrochloride hydrate
12 Trypticase peptone 2 G_PER_L
13 Na2-fumarate
C4H2O4.2Na 160.036 g/mol
3.2 G_PER_L CHEBI:115156
disodium fumarate
14 Na2CO3
CO3.2Na 105.988 g/mol
1 G_PER_L CHEBI:29377
sodium carbonate
15 Na2S x 9 H2O
9H2O.2Na.S 240.182 g/mol
0.5 G_PER_L CHEBI:76209
sodium sulfide nonahydrate
16 HCl
HCl 36.461 g/mol
2.5 G_PER_L CHEBI:17883
hydrogen chloride
17 FeCl2 x 4 H2O
Cl2Fe.4H2O 198.811 g/mol
1.5 G_PER_L CHEBI:86249
iron dichloride tetrahydrate
18 ZnCl2
2Cl.Zn 136.296 g/mol
0.07 G_PER_L CHEBI:49976
zinc dichloride
19 MnCl2 x 4 H2O
Cl2Mn.4H2O 197.904 g/mol
0.1 G_PER_L CHEBI:86368
manganese(II) chloride tetrahydrate
20 H3BO3
H3BO3 61.833 g/mol
0.006 G_PER_L CHEBI:33118
boric acid
21 CoCl2 x 6 H2O
2Cl.Co.6H2O 237.929 g/mol
0.19 G_PER_L CHEBI:53503
cobalt chloride hexahydrate
22 CuCl2 x 2 H2O
Cl2Cu.2H2O 170.482 g/mol
0.002 G_PER_L CHEBI:86318
copper(II) chloride dihydrate
23 NiCl2 x 6 H2O
Cl2Ni 129.599 g/mol
0.024 G_PER_L CHEBI:34887
nickel dichloride
24 Na2MoO4 x 2 H2O
2H2O.MoO4.2Na 241.946 g/mol
0.036 G_PER_L CHEBI:75213
sodium molybdate dihydrate

Preparation

  1. Dissolve ingredients (except sulfur, cysteine, peptone, fumarate, carbonate and sulfide) and sparge medium with 80% N2 and 20% CO2 gas mixture for 30 - 45 min to make it anoxic. Add and dissove cysteine, then dispense under 80% N2 and 20% CO2 gas atmosphere into anoxic Hungate-type tubes or serum vials containing already the appropriate amount of sulfur and autoclave at 121°C for 20 min. Add peptone, fumarate and sulfide from sterile anoxic stock solutions prepared under 100% N2 gas and carbonate from a sterile anoxic stock solution prepared under 80% N2 and 20% CO2 gas mixture. Adjust pH of the complete medium to 7.0.
  2. First dissolve FeCl2 in the HCl, then dilute in water, add and dissolve the other salts. Finally make up to 1000.00 ml.

Target organisms

No target organisms are recorded.

Provenance

TimestampCuratorActionNotes
2026-01-26T17:53:14.110773Z mediadive-import Imported from MediaDive Source: DSMZ, ID: 1328
2026-03-13T06:18:58.347640Z culturemech-id-assigner-v1.0 Assigned CultureMech ID Assigned stable identifier: CultureMech:000785
2026-03-17T06:30:23.000000Z mediaingredientmech-enrichment-v1.0 Added MediaIngredientMech links Linked ingredients to MediaIngredientMech identifiers
2026-03-17T19:45:09.744865+00:00 normalize_media_names_to_snake_case.py NAME_NORMALIZED Normalized name from "DEFLUVIITOGA MEDIUM" to "defluviitoga_medium" for consistent matching
2026-06-05T07:40:55.587743+00:00 mim-legacy-to-chebi-migration-v1.0 Refreshed legacy MediaIngredientMech links to CHEBI keying Replaced 21 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme.
2026-06-10T07:46:55.194148+00:00 primary-term-label-canonicalization-v1.0 Canonicalized primary term label to OBO canonical (OAK) 6 primary term(s); ingredient name resolved by OAK sqlite:obo:chebi.