algoriphagus_aquaeductus_medium

CultureMech:000789 bacterial COMPLEX SOLID_AGAR
Original name: ALGORIPHAGUS AQUAEDUCTUS MEDIUM

Overview

External ontology
mediadive.medium:1332 (source link unavailable) — ALGORIPHAGUS AQUAEDUCTUS MEDIUM
Applications
  • Microbial cultivation
pH
8.0
Notes
Source: DSMZ | Link: https://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium1332.pdf

Composition

flowchart LR
    CultureMech_000789["algoriphagus_aquaeductus_medium"]
    ing0_Meat_extract["Meat extract"]
    CultureMech_000789 --> ing0_Meat_extract
    ing1_NH4Cl["NH4Cl"]
    CultureMech_000789 --> ing1_NH4Cl
    CHEBI_31206["CHEBI:31206"]
    ing1_NH4Cl -.-> CHEBI_31206
    ing2_MgSO4_x_7_H2O["MgSO4 x 7 H2O"]
    CultureMech_000789 --> ing2_MgSO4_x_7_H2O
    CHEBI_31795["CHEBI:31795"]
    ing2_MgSO4_x_7_H2O -.-> CHEBI_31795
    ing3_CaCl2_x_2_H2O["CaCl2 x 2 H2O"]
    CultureMech_000789 --> ing3_CaCl2_x_2_H2O
    CHEBI_86158["CHEBI:86158"]
    ing3_CaCl2_x_2_H2O -.-> CHEBI_86158
    ing4_KH2PO4["KH2PO4"]
    CultureMech_000789 --> ing4_KH2PO4
    CHEBI_63036["CHEBI:63036"]
    ing4_KH2PO4 -.-> CHEBI_63036
    ing5_Agar["Agar"]
    CultureMech_000789 --> ing5_Agar
    CHEBI_2509["CHEBI:2509"]
    ing5_Agar -.-> CHEBI_2509
    ing6_Na2_EDTA["Na2-EDTA"]
    CultureMech_000789 --> ing6_Na2_EDTA
    CHEBI_64734["CHEBI:64734"]
    ing6_Na2_EDTA -.-> CHEBI_64734
    ing7_CoCl2_x_6_H2O["CoCl2 x 6 H2O"]
    CultureMech_000789 --> ing7_CoCl2_x_6_H2O
    CHEBI_53503["CHEBI:53503"]
    ing7_CoCl2_x_6_H2O -.-> CHEBI_53503
    ing8_MnCl2_x_2_H2O["MnCl2 x 2 H2O"]
    CultureMech_000789 --> ing8_MnCl2_x_2_H2O
    CHEBI_131395["CHEBI:131395"]
    ing8_MnCl2_x_2_H2O -.-> CHEBI_131395
    ing9_ZnSO4_x_7_H2O["ZnSO4 x 7 H2O"]
    CultureMech_000789 --> ing9_ZnSO4_x_7_H2O
    CHEBI_32312["CHEBI:32312"]
    ing9_ZnSO4_x_7_H2O -.-> CHEBI_32312
    ing10_NiCl2_x_6_H2O["NiCl2 x 6 H2O"]
    CultureMech_000789 --> ing10_NiCl2_x_6_H2O
    CHEBI_34887["CHEBI:34887"]
    ing10_NiCl2_x_6_H2O -.-> CHEBI_34887
    ing11_Na2MoO4_x_2_H2O["Na2MoO4 x 2 H2O"]
    CultureMech_000789 --> ing11_Na2MoO4_x_2_H2O
    CHEBI_75213["CHEBI:75213"]
    ing11_Na2MoO4_x_2_H2O -.-> CHEBI_75213
    ing12_H3BO3["H3BO3"]
    CultureMech_000789 --> ing12_H3BO3
    CHEBI_33118["CHEBI:33118"]
    ing12_H3BO3 -.-> CHEBI_33118
    ing13_CuCl2_x_2_H2O["CuCl2 x 2 H2O"]
    CultureMech_000789 --> ing13_CuCl2_x_2_H2O
    CHEBI_86318["CHEBI:86318"]
    ing13_CuCl2_x_2_H2O -.-> CHEBI_86318
    ing14_p_Aminobenzoic_acid["p-Aminobenzoic acid"]
    CultureMech_000789 --> ing14_p_Aminobenzoic_acid
    CHEBI_30753["CHEBI:30753"]
    ing14_p_Aminobenzoic_acid -.-> CHEBI_30753
    ing15_D_____biotin["D-(+)-biotin"]
    CultureMech_000789 --> ing15_D_____biotin
    CHEBI_15956["CHEBI:15956"]
    ing15_D_____biotin -.-> CHEBI_15956
    ing16_Calcium_pantothenate["Calcium pantothenate"]
    CultureMech_000789 --> ing16_Calcium_pantothenate
    CHEBI_31345["CHEBI:31345"]
    ing16_Calcium_pantothenate -.-> CHEBI_31345

Ingredients (17)

# Ingredient Concentration Ontology Notes
1 Meat extract 20 G_PER_L
2 NH4Cl
Cl.H4N 53.492 g/mol
1 G_PER_L CHEBI:31206
ammonium chloride
3 MgSO4 x 7 H2O
7H2O.Mg.O4S 246.473 g/mol
0.2 G_PER_L CHEBI:31795
magnesium sulfate heptahydrate
4 CaCl2 x 2 H2O
Ca.2Cl.2H2O 147.014 g/mol
0.01 G_PER_L CHEBI:86158
calcium chloride dihydrate
5 KH2PO4
H2O4P.K 136.084 g/mol
0.5 G_PER_L CHEBI:63036
potassium dihydrogen phosphate
6 Agar 15 G_PER_L CHEBI:2509
agar
 (for solid medium)
7 Na2-EDTA
C10H14N2O8.2Na 336.208 g/mol
5.2 G_PER_L CHEBI:64734
EDTA disodium salt (anhydrous)
8 CoCl2 x 6 H2O
2Cl.Co.6H2O 237.929 g/mol
0.19 G_PER_L CHEBI:53503
cobalt chloride hexahydrate
9 MnCl2 x 2 H2O
Cl2Mn.2H2O 161.874 g/mol
0.1 G_PER_L CHEBI:131395
manganese(II) chloride dihydrate
10 ZnSO4 x 7 H2O
7H2O.O4S.Zn 287.558 g/mol
0.15 G_PER_L CHEBI:32312
zinc sulfate heptahydrate
11 NiCl2 x 6 H2O
Cl2Ni 129.599 g/mol
0.024 G_PER_L CHEBI:34887
nickel dichloride
12 Na2MoO4 x 2 H2O
2H2O.MoO4.2Na 241.946 g/mol
0.036 G_PER_L CHEBI:75213
sodium molybdate dihydrate
13 H3BO3
H3BO3 61.833 g/mol
0.062 G_PER_L CHEBI:33118
boric acid
14 CuCl2 x 2 H2O
Cl2Cu.2H2O 170.482 g/mol
0.017 G_PER_L CHEBI:86318
copper(II) chloride dihydrate
15 p-Aminobenzoic acid
C7H7NO2 137.138 g/mol
0.08 G_PER_L CHEBI:30753
4-aminobenzoic acid
16 D-(+)-biotin
C10H16N2O3S 244.316 g/mol
0.02 G_PER_L CHEBI:15956
biotin
17 Calcium pantothenate
2C9H16NO5.Ca 476.536 g/mol
0.1 G_PER_L CHEBI:31345
Calcium pantothenate

Preparation

  1. Adjust pH to 8.0 before autoclaving. The medium may be solidified by adding 15 g/l agar.
  2. Adjust pH to 6.8.
  3. Filter sterilise and add to the medium after it has been autoclaved.

Solutions

Seven vitamins solution

Dose: 3 ML_PER_L

Stock composition
IngredientConcentrationNotes
Vitamin B12 0.1 G_PER_L
Nicotinic acid 0.2 G_PER_L
Pyridoxine hydrochloride 0.3 G_PER_L
Thiamine-HCl x 2 H2O 0.2 G_PER_L

Trace element solution SL-8

Dose: 1 ML_PER_L

Stock composition
IngredientConcentrationNotes
FeSO4 x 7 H2O 2 G_PER_L

Target organisms

No target organisms are recorded.

Provenance

TimestampCuratorActionNotes
2026-01-26T17:53:14.123578Z mediadive-import Imported from MediaDive Source: DSMZ, ID: 1332
2026-03-13T06:18:58.383026Z culturemech-id-assigner-v1.0 Assigned CultureMech ID Assigned stable identifier: CultureMech:000789
2026-03-17T06:30:52.000000Z mediaingredientmech-enrichment-v1.0 Added MediaIngredientMech links Linked ingredients to MediaIngredientMech identifiers
2026-03-17T19:45:09.783887+00:00 normalize_media_names_to_snake_case.py NAME_NORMALIZED Normalized name from "ALGORIPHAGUS AQUAEDUCTUS MEDIUM" to "algoriphagus_aquaeductus_medium" for consistent matching
2026-06-05T07:40:47.727332+00:00 mim-legacy-to-chebi-migration-v1.0 Refreshed legacy MediaIngredientMech links to CHEBI keying Replaced 20 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme.
2026-06-10T07:46:52.740659+00:00 primary-term-label-canonicalization-v1.0 Canonicalized primary term label to OBO canonical (OAK) 3 primary term(s); ingredient name resolved by OAK sqlite:obo:chebi.
2026-08-07T07:05:21.596757+00:00 apply_cocktail_nesting.py NESTED_FLATTENED_COCKTAIL Moved 5 stock-strength component(s) out of ingredients into 2 solution(s): 4 -> 'Seven vitamins solution' @ 3 ml/l; 1 -> 'Trace element solution SL-8' @ 1 ml/l. Each moved component matched the MediaDive stock by name AND value; bulk ingredients sharing a name were left in place (#150).