lyu03_medium

CultureMech:000802 bacterial COMPLEX LIQUID
Original name: LYU03 Medium

Overview

External ontology
mediadive.medium:1344 (source link unavailable) — LYU03 Medium
Applications
  • Microbial cultivation
pH
8.3
Notes
Source: DSMZ | Link: https://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium1344.pdf

Composition

flowchart LR
    CultureMech_000802["lyu03_medium"]
    ing0_Taps["Taps"]
    CultureMech_000802 --> ing0_Taps
    ing1_Yeast_extract["Yeast extract"]
    CultureMech_000802 --> ing1_Yeast_extract
    ing2_Sodium_acetate["Sodium acetate"]
    CultureMech_000802 --> ing2_Sodium_acetate
    CHEBI_32954["CHEBI:32954"]
    ing2_Sodium_acetate -.-> CHEBI_32954
    ing3_Disodium_succinate["Disodium succinate"]
    CultureMech_000802 --> ing3_Disodium_succinate
    CHEBI_63675["CHEBI:63675"]
    ing3_Disodium_succinate -.-> CHEBI_63675
    ing4_Na2S2O3_x_5_H2O["Na2S2O3 x 5 H2O"]
    CultureMech_000802 --> ing4_Na2S2O3_x_5_H2O
    CHEBI_32150["CHEBI:32150"]
    ing4_Na2S2O3_x_5_H2O -.-> CHEBI_32150
    ing5_CaCl2_x_2_H2O["CaCl2 x 2 H2O"]
    CultureMech_000802 --> ing5_CaCl2_x_2_H2O
    CHEBI_86158["CHEBI:86158"]
    ing5_CaCl2_x_2_H2O -.-> CHEBI_86158
    ing6_MgSO4_x_7_H2O["MgSO4 x 7 H2O"]
    CultureMech_000802 --> ing6_MgSO4_x_7_H2O
    CHEBI_31795["CHEBI:31795"]
    ing6_MgSO4_x_7_H2O -.-> CHEBI_31795
    ing7_NH4Cl["NH4Cl"]
    CultureMech_000802 --> ing7_NH4Cl
    CHEBI_31206["CHEBI:31206"]
    ing7_NH4Cl -.-> CHEBI_31206
    ing8_KH2PO4["KH2PO4"]
    CultureMech_000802 --> ing8_KH2PO4
    CHEBI_63036["CHEBI:63036"]
    ing8_KH2PO4 -.-> CHEBI_63036
    ing9_Na2_EDTA["Na2-EDTA"]
    CultureMech_000802 --> ing9_Na2_EDTA
    CHEBI_64734["CHEBI:64734"]
    ing9_Na2_EDTA -.-> CHEBI_64734
    ing10_Vitamin_B12["Vitamin B12"]
    CultureMech_000802 --> ing10_Vitamin_B12
    CHEBI_176843["CHEBI:176843"]
    ing10_Vitamin_B12 -.-> CHEBI_176843
    ing11_EDTA["EDTA"]
    CultureMech_000802 --> ing11_EDTA
    CHEBI_4735["CHEBI:4735"]
    ing11_EDTA -.-> CHEBI_4735
    ing12_CoCl2_x_6_H2O["CoCl2 x 6 H2O"]
    CultureMech_000802 --> ing12_CoCl2_x_6_H2O
    CHEBI_53503["CHEBI:53503"]
    ing12_CoCl2_x_6_H2O -.-> CHEBI_53503
    ing13_MnCl2_x_4_H2O["MnCl2 x 4 H2O"]
    CultureMech_000802 --> ing13_MnCl2_x_4_H2O
    CHEBI_86368["CHEBI:86368"]
    ing13_MnCl2_x_4_H2O -.-> CHEBI_86368
    ing14_FeCl2_x_4_H2O["FeCl2 x 4 H2O"]
    CultureMech_000802 --> ing14_FeCl2_x_4_H2O
    CHEBI_86249["CHEBI:86249"]
    ing14_FeCl2_x_4_H2O -.-> CHEBI_86249
    ing15_H3BO3["H3BO3"]
    CultureMech_000802 --> ing15_H3BO3
    CHEBI_33118["CHEBI:33118"]
    ing15_H3BO3 -.-> CHEBI_33118
    ing16_CuCl2_x_2_H2O["CuCl2 x 2 H2O"]
    CultureMech_000802 --> ing16_CuCl2_x_2_H2O
    CHEBI_86318["CHEBI:86318"]
    ing16_CuCl2_x_2_H2O -.-> CHEBI_86318
    ing17_Na2MoO4_x_2_H2O["Na2MoO4 x 2 H2O"]
    CultureMech_000802 --> ing17_Na2MoO4_x_2_H2O
    CHEBI_75213["CHEBI:75213"]
    ing17_Na2MoO4_x_2_H2O -.-> CHEBI_75213
    ing18_NiCl2_x_6_H2O["NiCl2 x 6 H2O"]
    CultureMech_000802 --> ing18_NiCl2_x_6_H2O
    CHEBI_34887["CHEBI:34887"]
    ing18_NiCl2_x_6_H2O -.-> CHEBI_34887
    ing19_ZnCl2["ZnCl2"]
    CultureMech_000802 --> ing19_ZnCl2
    CHEBI_49976["CHEBI:49976"]
    ing19_ZnCl2 -.-> CHEBI_49976
    ing20_VOSO4_x_2_H2O["VOSO4 x 2 H2O"]
    CultureMech_000802 --> ing20_VOSO4_x_2_H2O
    CHEBI_87009["CHEBI:87009"]
    ing20_VOSO4_x_2_H2O -.-> CHEBI_87009
    ing21_Na2WO4_x_2_H2O["Na2WO4 x 2 H2O"]
    CultureMech_000802 --> ing21_Na2WO4_x_2_H2O
    CHEBI_63939["CHEBI:63939"]
    ing21_Na2WO4_x_2_H2O -.-> CHEBI_63939
    ing22_NaHSeO3["NaHSeO3"]
    CultureMech_000802 --> ing22_NaHSeO3
    ing23_Na2S_x_9_H2O["Na2S x 9 H2O"]
    CultureMech_000802 --> ing23_Na2S_x_9_H2O
    CHEBI_76209["CHEBI:76209"]
    ing23_Na2S_x_9_H2O -.-> CHEBI_76209
    ing24_NaHCO3["NaHCO3"]
    CultureMech_000802 --> ing24_NaHCO3
    CHEBI_32139["CHEBI:32139"]
    ing24_NaHCO3 -.-> CHEBI_32139
    ing25_Biotin["Biotin"]
    CultureMech_000802 --> ing25_Biotin
    CHEBI_15956["CHEBI:15956"]
    ing25_Biotin -.-> CHEBI_15956
    ing26_Pyridoxamine_hydrochloride["Pyridoxamine hydrochloride"]
    CultureMech_000802 --> ing26_Pyridoxamine_hydrochloride
    CHEBI_131531["CHEBI:131531"]
    ing26_Pyridoxamine_hydrochloride -.-> CHEBI_131531
    ing27_p_Aminobenzoic_acid["p-Aminobenzoic acid"]
    CultureMech_000802 --> ing27_p_Aminobenzoic_acid
    CHEBI_30753["CHEBI:30753"]
    ing27_p_Aminobenzoic_acid -.-> CHEBI_30753
    ing28_Niacin["Niacin"]
    CultureMech_000802 --> ing28_Niacin
    CHEBI_15940["CHEBI:15940"]
    ing28_Niacin -.-> CHEBI_15940
    ing29_Thiamine_HCl["Thiamine HCl"]
    CultureMech_000802 --> ing29_Thiamine_HCl
    CHEBI_49105["CHEBI:49105"]
    ing29_Thiamine_HCl -.-> CHEBI_49105

Ingredients (30)

# Ingredient Concentration Ontology Notes
1 Taps 17.4 G_PER_L
2 Yeast extract 0.1 G_PER_L
3 Sodium acetate
C2H3O2.Na 82.034 g/mol
1 G_PER_L CHEBI:32954
sodium acetate
4 Disodium succinate
C4H4O4.2Na 162.052 g/mol
1 G_PER_L CHEBI:63675
sodium succinate (anhydrous)
5 Na2S2O3 x 5 H2O
5H2O.2Na.O3S2 248.186 g/mol
0.1 G_PER_L CHEBI:32150
sodium thiosulfate pentahydrate
6 CaCl2 x 2 H2O
Ca.2Cl.2H2O 147.014 g/mol
0.075 G_PER_L CHEBI:86158
calcium chloride dihydrate
7 MgSO4 x 7 H2O
7H2O.Mg.O4S 246.473 g/mol
0.2 G_PER_L CHEBI:31795
magnesium sulfate heptahydrate
8 NH4Cl
Cl.H4N 53.492 g/mol
0.5 G_PER_L CHEBI:31206
ammonium chloride
9 KH2PO4
H2O4P.K 136.084 g/mol
0.5 G_PER_L CHEBI:63036
potassium dihydrogen phosphate
10 Na2-EDTA
C10H14N2O8.2Na 336.208 g/mol
0.005 G_PER_L CHEBI:64734
EDTA disodium salt (anhydrous)
11 Vitamin B12 0.05004 G_PER_L CHEBI:176843
vitamin B12
 [Merged 2 duplicates: 4e-05, 0.05]
12 EDTA
C10H16N2O8 292.244 g/mol
5.2 G_PER_L CHEBI:4735
ethylenediaminetetraacetic acid
13 CoCl2 x 6 H2O
2Cl.Co.6H2O 237.929 g/mol
0.19 G_PER_L CHEBI:53503
cobalt chloride hexahydrate
14 MnCl2 x 4 H2O
Cl2Mn.4H2O 197.904 g/mol
0.1 G_PER_L CHEBI:86368
manganese(II) chloride tetrahydrate
15 FeCl2 x 4 H2O
Cl2Fe.4H2O 198.811 g/mol
1.5 G_PER_L CHEBI:86249
iron dichloride tetrahydrate
16 H3BO3
H3BO3 61.833 g/mol
0.006 G_PER_L CHEBI:33118
boric acid
17 CuCl2 x 2 H2O
Cl2Cu.2H2O 170.482 g/mol
0.017 G_PER_L CHEBI:86318
copper(II) chloride dihydrate
18 Na2MoO4 x 2 H2O
2H2O.MoO4.2Na 241.946 g/mol
0.188 G_PER_L CHEBI:75213
sodium molybdate dihydrate
19 NiCl2 x 6 H2O
Cl2Ni 129.599 g/mol
0.025 G_PER_L CHEBI:34887
nickel dichloride
20 ZnCl2
2Cl.Zn 136.296 g/mol
0.07 G_PER_L CHEBI:49976
zinc dichloride
21 VOSO4 x 2 H2O
2H2O.O4S.OV 199.036 g/mol
0.03 G_PER_L CHEBI:87009
vanadyl sulfate dihydrate
22 Na2WO4 x 2 H2O
2H2O.2Na.O4W 329.846 g/mol
0.002 G_PER_L CHEBI:63939
sodium tungstate dihydrate
23 NaHSeO3 0.002 G_PER_L
24 Na2S x 9 H2O
9H2O.2Na.S 240.182 g/mol
15 G_PER_L CHEBI:76209
sodium sulfide nonahydrate
25 NaHCO3
CHO3.Na 84.006 g/mol
83.3333 G_PER_L CHEBI:32139
sodium hydrogencarbonate
26 Biotin
C10H16N2O3S 244.316 g/mol
0.05 G_PER_L CHEBI:15956
biotin
27 Pyridoxamine hydrochloride
C8H12N2O2.HCl 204.657 g/mol
0.1 G_PER_L CHEBI:131531
pyridoxamine hydrochloride
28 p-Aminobenzoic acid
C7H7NO2 137.138 g/mol
0.3 G_PER_L CHEBI:30753
4-aminobenzoic acid
29 Niacin
C6H5NO2 123.111 g/mol
0.5 G_PER_L CHEBI:15940
nicotinic acid
30 Thiamine HCl
C12H17N4OS.Cl.HCl 337.276 g/mol
0.5 G_PER_L CHEBI:49105
thiamine hydrochloride

Preparation

  1. pH 8.3
  2. Mix ingredients vitamin B12, vitamin mixture, bicarbonate solution and neutralized sulfide solution. Dispense the medium into suitable culture vessels under a stream of nitrogen gas, seal with butyl rubber stoppers and autoclave at 121°C for 15 min. Separately autoclave neutralized sulfide solution under a N2 atmosphere. Sterilize Vitamin B12 solution, vitamin mixture, bicarbonate solution by filtration. Prior to inoculation, aseptically and anaerobically add vitamin B12 solution, vitamin mixture, bicarbonate solution and neutralized sulfide solution.
  3. EDTA should be fully dissolved before adding the other components. Store at 4°C.
  4. The sulfide solution is prepared in a vessel sealed with a butyl rubber.
  5. Autoclave at 121°C for 15 min under a N2 atmosphere. After cooling to room temperature the pH is adjusted to about 7.3 by adding of sterile 2N H2SO4 drop-wise with a syringe without opening the bottle. The vessel should be shaken continuously to avoid precipitation of elemental sulfur. The final solution should be transparent yellow.

Target organisms

No target organisms are recorded.

Provenance

TimestampCuratorActionNotes
2026-01-26T17:53:14.152403Z mediadive-import Imported from MediaDive Source: DSMZ, ID: 1344
2026-03-13T03:09:31.565640Z data-quality-cleanup-v1.0 Data quality fixes Merged 1 duplicate ingredient(s)
2026-03-13T06:18:58.472364Z culturemech-id-assigner-v1.0 Assigned CultureMech ID Assigned stable identifier: CultureMech:000802
2026-03-17T06:30:41.000000Z mediaingredientmech-enrichment-v1.0 Added MediaIngredientMech links Linked ingredients to MediaIngredientMech identifiers
2026-03-17T19:45:09.889910+00:00 normalize_media_names_to_snake_case.py NAME_NORMALIZED Normalized name from "LYU03 Medium" to "lyu03_medium" for consistent matching
2026-06-05T07:41:11.594512+00:00 mim-legacy-to-chebi-migration-v1.0 Refreshed legacy MediaIngredientMech links to CHEBI keying Replaced 27 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme.
2026-06-10T07:47:00.626856+00:00 primary-term-label-canonicalization-v1.0 Canonicalized primary term label to OBO canonical (OAK) 3 primary term(s); ingredient name resolved by OAK sqlite:obo:chebi.