haliscomenobacter_medium

CultureMech:000808 bacterial COMPLEX LIQUID
Original name: HALISCOMENOBACTER MEDIUM

Overview

External ontology
mediadive.medium:134 (source link unavailable) — HALISCOMENOBACTER MEDIUM
Applications
  • Microbial cultivation
pH
7.5
Notes
Source: DSMZ | Link: https://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium134.pdf Commercial Product: Tryptic Soy Broth (TSB) / Tryptic Soy Agar (TSA) Supplier: Multiple suppliers (BD, Sigma, Neogen) Catalog: Multiple (22092 Sigma, 211825 BD) Source: https://en.wikipedia.org/wiki/Tryptic_soy_broth Accessed: 2026-03-28 Constituent composition researched from product specification.

Composition

flowchart LR
    CultureMech_000808["haliscomenobacter_medium"]
    ing0_KH2PO4["KH2PO4"]
    CultureMech_000808 --> ing0_KH2PO4
    CHEBI_63036["CHEBI:63036"]
    ing0_KH2PO4 -.-> CHEBI_63036
    ing1_K2HPO4["K2HPO4"]
    CultureMech_000808 --> ing1_K2HPO4
    CHEBI_131527["CHEBI:131527"]
    ing1_K2HPO4 -.-> CHEBI_131527
    ing2_Na2HPO4_x_2_H2O["Na2HPO4 x 2 H2O"]
    CultureMech_000808 --> ing2_Na2HPO4_x_2_H2O
    CHEBI_91258["CHEBI:91258"]
    ing2_Na2HPO4_x_2_H2O -.-> CHEBI_91258
    ing3_CaCl2_x_2_H2O["CaCl2 x 2 H2O"]
    CultureMech_000808 --> ing3_CaCl2_x_2_H2O
    CHEBI_86158["CHEBI:86158"]
    ing3_CaCl2_x_2_H2O -.-> CHEBI_86158
    ing4_MgSO4_x_7_H2O["MgSO4 x 7 H2O"]
    CultureMech_000808 --> ing4_MgSO4_x_7_H2O
    CHEBI_31795["CHEBI:31795"]
    ing4_MgSO4_x_7_H2O -.-> CHEBI_31795
    ing5_FeCl3_x_6_H2O["FeCl3 x 6 H2O"]
    CultureMech_000808 --> ing5_FeCl3_x_6_H2O
    CHEBI_86254["CHEBI:86254"]
    ing5_FeCl3_x_6_H2O -.-> CHEBI_86254
    ing6_MnSO4_x_H2O["MnSO4 x H2O"]
    CultureMech_000808 --> ing6_MnSO4_x_H2O
    CHEBI_86364["CHEBI:86364"]
    ing6_MnSO4_x_H2O -.-> CHEBI_86364
    ing7_Glutamic_acid["Glutamic acid"]
    CultureMech_000808 --> ing7_Glutamic_acid
    CHEBI_18237["CHEBI:18237"]
    ing7_Glutamic_acid -.-> CHEBI_18237
    ing8_Pancreatic_digest_of_casein["Pancreatic digest of casein"]
    CultureMech_000808 --> ing8_Pancreatic_digest_of_casein
    ing9_Peptic_digest_of_soybean_meal["Peptic digest of soybean meal"]
    CultureMech_000808 --> ing9_Peptic_digest_of_soybean_meal
    ing10_Glucose["Glucose"]
    CultureMech_000808 --> ing10_Glucose
    CHEBI_17234["CHEBI:17234"]
    ing10_Glucose -.-> CHEBI_17234
    ing11_Sodium_chloride["Sodium chloride"]
    CultureMech_000808 --> ing11_Sodium_chloride
    CHEBI_26710["CHEBI:26710"]
    ing11_Sodium_chloride -.-> CHEBI_26710
    ing12_Dipotassium_phosphate["Dipotassium phosphate"]
    CultureMech_000808 --> ing12_Dipotassium_phosphate
    ing12_Dipotassium_phosphate -.-> CHEBI_131527
    ing13_Agar["Agar"]
    CultureMech_000808 --> ing13_Agar
    CHEBI_2509["CHEBI:2509"]
    ing13_Agar -.-> CHEBI_2509
    ing14_Thiamine_HCl_x_2_H2O["Thiamine-HCl x 2 H2O"]
    CultureMech_000808 --> ing14_Thiamine_HCl_x_2_H2O
    CHEBI_132751["CHEBI:132751"]
    ing14_Thiamine_HCl_x_2_H2O -.-> CHEBI_132751
    ing15_Vitamin_B12["Vitamin B12"]
    CultureMech_000808 --> ing15_Vitamin_B12
    CHEBI_176843["CHEBI:176843"]
    ing15_Vitamin_B12 -.-> CHEBI_176843
    ing16_Glucose["Glucose"]
    CultureMech_000808 --> ing16_Glucose
    ing16_Glucose -.-> CHEBI_17234
    ing17_ZnSO4_x_7_H2O["ZnSO4 x 7 H2O"]
    CultureMech_000808 --> ing17_ZnSO4_x_7_H2O
    CHEBI_32312["CHEBI:32312"]
    ing17_ZnSO4_x_7_H2O -.-> CHEBI_32312
    ing18_MnCl2_x_4_H2O["MnCl2 x 4 H2O"]
    CultureMech_000808 --> ing18_MnCl2_x_4_H2O
    CHEBI_86368["CHEBI:86368"]
    ing18_MnCl2_x_4_H2O -.-> CHEBI_86368
    ing19_H3BO3["H3BO3"]
    CultureMech_000808 --> ing19_H3BO3
    CHEBI_33118["CHEBI:33118"]
    ing19_H3BO3 -.-> CHEBI_33118
    ing20_CoCl2_x_6_H2O["CoCl2 x 6 H2O"]
    CultureMech_000808 --> ing20_CoCl2_x_6_H2O
    CHEBI_53503["CHEBI:53503"]
    ing20_CoCl2_x_6_H2O -.-> CHEBI_53503
    ing21_CuCl2_x_2_H2O["CuCl2 x 2 H2O"]
    CultureMech_000808 --> ing21_CuCl2_x_2_H2O
    CHEBI_86318["CHEBI:86318"]
    ing21_CuCl2_x_2_H2O -.-> CHEBI_86318
    ing22_NiCl2_x_6_H2O["NiCl2 x 6 H2O"]
    CultureMech_000808 --> ing22_NiCl2_x_6_H2O
    CHEBI_34887["CHEBI:34887"]
    ing22_NiCl2_x_6_H2O -.-> CHEBI_34887
    ing23_Na2MoO4_x_2_H2O["Na2MoO4 x 2 H2O"]
    CultureMech_000808 --> ing23_Na2MoO4_x_2_H2O
    CHEBI_75213["CHEBI:75213"]
    ing23_Na2MoO4_x_2_H2O -.-> CHEBI_75213

Ingredients (24)

# Ingredient Concentration Ontology Notes
1 KH2PO4
H2O4P.K 136.084 g/mol
0.027 G_PER_L CHEBI:63036
potassium dihydrogen phosphate
2 K2HPO4
HO4P.2K 174.174 g/mol
0.04 G_PER_L CHEBI:131527
dipotassium hydrogen phosphate
3 Na2HPO4 x 2 H2O
2H2O.HO4P.2Na 177.988 g/mol
0.04 G_PER_L CHEBI:91258
disodium hydrogenphosphate dihydrate
4 CaCl2 x 2 H2O
Ca.2Cl.2H2O 147.014 g/mol
0.05 G_PER_L CHEBI:86158
calcium chloride dihydrate
5 MgSO4 x 7 H2O
7H2O.Mg.O4S 246.473 g/mol
0.075 G_PER_L CHEBI:31795
magnesium sulfate heptahydrate
6 FeCl3 x 6 H2O
Cl3Fe.6H2O 270.294 g/mol
0.005 G_PER_L CHEBI:86254
iron trichloride hexahydrate
7 MnSO4 x H2O
H2O.Mn.O4S 169.016 g/mol
0.003 G_PER_L CHEBI:86364
manganese(II) sulfate monohydrate
8 Glutamic acid
C5H9NO4 147.13 g/mol
1.31 G_PER_L CHEBI:18237
glutamic acid
9 Pancreatic digest of casein
Multiple suppliers (BD, Sigma, Neogen) Tryptic Soy Broth (TSB) / Tryptic Soy Agar (TSA) Multiple (22092 Sigma, 211825 BD)
17.0 G_PER_L MICRO:0000182 (source link unavailable)
tryptone
 Pancreatic enzymatic digest of casein providing amino acids and peptides; Constituent of Tryptic Soy Broth (TSB) / Tryptic Soy Agar (TSA) commercial product
10 Peptic digest of soybean meal
Multiple suppliers (BD, Sigma, Neogen) Tryptic Soy Broth (TSB) / Tryptic Soy Agar (TSA) Multiple (22092 Sigma, 211825 BD)
3.0 G_PER_L FOODON:03315720
vegetable protein, hydrolyzed
 Peptic digest of soybean meal providing additional peptides and carbohydrates; Constituent of Tryptic Soy Broth (TSB) / Tryptic Soy Agar (TSA) commercial product
11 Glucose
C6H12O6 180.156 g/mol
Multiple suppliers (BD, Sigma, Neogen) Tryptic Soy Broth (TSB) / Tryptic Soy Agar (TSA) Multiple (22092 Sigma, 211825 BD)
2.5 G_PER_L CHEBI:17234
glucose
 Carbon/energy source; Constituent of Tryptic Soy Broth (TSB) / Tryptic Soy Agar (TSA) commercial product
12 Sodium chloride
Cl.Na 58.443 g/mol
Multiple suppliers (BD, Sigma, Neogen) Tryptic Soy Broth (TSB) / Tryptic Soy Agar (TSA) Multiple (22092 Sigma, 211825 BD)
5.0 G_PER_L CHEBI:26710
sodium chloride
 Maintains osmotic equilibrium; Constituent of Tryptic Soy Broth (TSB) / Tryptic Soy Agar (TSA) commercial product
13 Dipotassium phosphate
HO4P.2K 174.174 g/mol
Multiple suppliers (BD, Sigma, Neogen) Tryptic Soy Broth (TSB) / Tryptic Soy Agar (TSA) Multiple (22092 Sigma, 211825 BD)
2.5 G_PER_L CHEBI:131527
dipotassium hydrogen phosphate
 pH buffer; Constituent of Tryptic Soy Broth (TSB) / Tryptic Soy Agar (TSA) commercial product
14 Agar
Multiple suppliers (BD, Sigma, Neogen) Tryptic Soy Broth (TSB) / Tryptic Soy Agar (TSA) Multiple (22092 Sigma, 211825 BD)
15.0 G_PER_L CHEBI:2509
agar
 Solidifying agent (for TSA only, not in TSB); Constituent of Tryptic Soy Broth (TSB) / Tryptic Soy Agar (TSA) commercial product
15 Thiamine-HCl x 2 H2O
C12H18N4OS.2Cl.2H2O 373.306 g/mol
0.0004 G_PER_L CHEBI:132751
thiamine hydrochloride dihydrate
16 Vitamin B12 1e-05 G_PER_L CHEBI:176843
vitamin B12
17 Glucose
C6H12O6 180.156 g/mol
2 G_PER_L CHEBI:17234
glucose
18 ZnSO4 x 7 H2O
7H2O.O4S.Zn 287.558 g/mol
0.1 G_PER_L CHEBI:32312
zinc sulfate heptahydrate
19 MnCl2 x 4 H2O
Cl2Mn.4H2O 197.904 g/mol
0.03 G_PER_L CHEBI:86368
manganese(II) chloride tetrahydrate
20 H3BO3
H3BO3 61.833 g/mol
0.3 G_PER_L CHEBI:33118
boric acid
21 CoCl2 x 6 H2O
2Cl.Co.6H2O 237.929 g/mol
0.2 G_PER_L CHEBI:53503
cobalt chloride hexahydrate
22 CuCl2 x 2 H2O
Cl2Cu.2H2O 170.482 g/mol
0.01 G_PER_L CHEBI:86318
copper(II) chloride dihydrate
23 NiCl2 x 6 H2O
Cl2Ni 129.599 g/mol
0.02 G_PER_L CHEBI:34887
nickel dichloride
24 Na2MoO4 x 2 H2O
2H2O.MoO4.2Na 241.946 g/mol
0.03 G_PER_L CHEBI:75213
sodium molybdate dihydrate

Preparation

  1. Adjust pH to 7.5. The vitamins are sterilized by filtration. The glucose is dissolved in 5 ml of distilled water and sterilized separately by autoclaving.

Target organisms

No target organisms are recorded.

Provenance

TimestampCuratorActionNotes
2026-01-26T17:53:10.269264Z mediadive-import Imported from MediaDive Source: DSMZ, ID: 134
2026-03-13T06:18:58.506656Z culturemech-id-assigner-v1.0 Assigned CultureMech ID Assigned stable identifier: CultureMech:000808
2026-03-17T06:30:42.000000Z mediaingredientmech-enrichment-v1.0 Added MediaIngredientMech links Linked ingredients to MediaIngredientMech identifiers
2026-03-17T19:45:09.928789+00:00 normalize_media_names_to_snake_case.py NAME_NORMALIZED Normalized name from "HALISCOMENOBACTER MEDIUM" to "haliscomenobacter_medium" for consistent matching
2026-06-05T07:41:07.221115+00:00 mim-legacy-to-chebi-migration-v1.0 Refreshed legacy MediaIngredientMech links to CHEBI keying Replaced 18 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme.
2026-06-10T07:46:59.329373+00:00 primary-term-label-canonicalization-v1.0 Canonicalized primary term label to OBO canonical (OAK) 3 primary term(s); ingredient name resolved by OAK sqlite:obo:chebi.