Overview
- External ontology
- mediadive.medium:1352 (source link unavailable) — FUCHSIELLA ALKALIACETIGENA MEDIUM
- Applications
-
- Microbial cultivation
- pH range
- {'min': 8.8, 'max': 9.5}
- Notes
- Source: DSMZ | Link: https://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium1352.pdf
Composition
flowchart LR
CultureMech_000811["fuchsiella_alkaliacetigena_medium"]
ing0_KH2PO4["KH2PO4"]
CultureMech_000811 --> ing0_KH2PO4
CHEBI_63036["CHEBI:63036"]
ing0_KH2PO4 -.-> CHEBI_63036
ing1_MgCl2_x_6_H2O["MgCl2 x 6 H2O"]
CultureMech_000811 --> ing1_MgCl2_x_6_H2O
CHEBI_86345["CHEBI:86345"]
ing1_MgCl2_x_6_H2O -.-> CHEBI_86345
ing2_NH4Cl["NH4Cl"]
CultureMech_000811 --> ing2_NH4Cl
CHEBI_31206["CHEBI:31206"]
ing2_NH4Cl -.-> CHEBI_31206
ing3_KCl["KCl"]
CultureMech_000811 --> ing3_KCl
CHEBI_32588["CHEBI:32588"]
ing3_KCl -.-> CHEBI_32588
ing4_NaCl["NaCl"]
CultureMech_000811 --> ing4_NaCl
CHEBI_26710["CHEBI:26710"]
ing4_NaCl -.-> CHEBI_26710
ing5_Na2CO3["Na2CO3"]
CultureMech_000811 --> ing5_Na2CO3
CHEBI_29377["CHEBI:29377"]
ing5_Na2CO3 -.-> CHEBI_29377
ing6_NaHCO3["NaHCO3"]
CultureMech_000811 --> ing6_NaHCO3
CHEBI_32139["CHEBI:32139"]
ing6_NaHCO3 -.-> CHEBI_32139
ing7_Resazurin["Resazurin"]
CultureMech_000811 --> ing7_Resazurin
CHEBI_8806["CHEBI:8806"]
ing7_Resazurin -.-> CHEBI_8806
ing8_Yeast_extract["Yeast extract"]
CultureMech_000811 --> ing8_Yeast_extract
ing9_Ethanol["Ethanol"]
CultureMech_000811 --> ing9_Ethanol
CHEBI_16236["CHEBI:16236"]
ing9_Ethanol -.-> CHEBI_16236
ing10_Na2S_x_9_H2O["Na2S x 9 H2O"]
CultureMech_000811 --> ing10_Na2S_x_9_H2O
CHEBI_76209["CHEBI:76209"]
ing10_Na2S_x_9_H2O -.-> CHEBI_76209
ing11_NaNO3["NaNO3"]
CultureMech_000811 --> ing11_NaNO3
CHEBI_63005["CHEBI:63005"]
ing11_NaNO3 -.-> CHEBI_63005
ing12_EDTA["EDTA"]
CultureMech_000811 --> ing12_EDTA
CHEBI_4735["CHEBI:4735"]
ing12_EDTA -.-> CHEBI_4735
ing13_FeSO4_x_7_H2O["FeSO4 x 7 H2O"]
CultureMech_000811 --> ing13_FeSO4_x_7_H2O
CHEBI_75836["CHEBI:75836"]
ing13_FeSO4_x_7_H2O -.-> CHEBI_75836
ing14_Biotin["Biotin"]
CultureMech_000811 --> ing14_Biotin
CHEBI_15956["CHEBI:15956"]
ing14_Biotin -.-> CHEBI_15956
ing15_Folic_acid["Folic acid"]
CultureMech_000811 --> ing15_Folic_acid
CHEBI_27470["CHEBI:27470"]
ing15_Folic_acid -.-> CHEBI_27470
ing16_Pyridoxine_hydrochloride["Pyridoxine hydrochloride"]
CultureMech_000811 --> ing16_Pyridoxine_hydrochloride
CHEBI_30961["CHEBI:30961"]
ing16_Pyridoxine_hydrochloride -.-> CHEBI_30961
ing17_Thiamine_HCl["Thiamine HCl"]
CultureMech_000811 --> ing17_Thiamine_HCl
CHEBI_49105["CHEBI:49105"]
ing17_Thiamine_HCl -.-> CHEBI_49105
ing18_Riboflavin["Riboflavin"]
CultureMech_000811 --> ing18_Riboflavin
CHEBI_17015["CHEBI:17015"]
ing18_Riboflavin -.-> CHEBI_17015
ing19_Nicotinic_acid["Nicotinic acid"]
CultureMech_000811 --> ing19_Nicotinic_acid
CHEBI_15940["CHEBI:15940"]
ing19_Nicotinic_acid -.-> CHEBI_15940
ing20_Calcium_D_____pantothenate["Calcium D-(+)-pantothenate"]
CultureMech_000811 --> ing20_Calcium_D_____pantothenate
CHEBI_31345["CHEBI:31345"]
ing20_Calcium_D_____pantothenate -.-> CHEBI_31345
ing21_Vitamin_B12["Vitamin B12"]
CultureMech_000811 --> ing21_Vitamin_B12
CHEBI_176843["CHEBI:176843"]
ing21_Vitamin_B12 -.-> CHEBI_176843
ing22_p_Aminobenzoic_acid["p-Aminobenzoic acid"]
CultureMech_000811 --> ing22_p_Aminobenzoic_acid
CHEBI_30753["CHEBI:30753"]
ing22_p_Aminobenzoic_acid -.-> CHEBI_30753
ing23__DL__alpha_Lipoic_acid["(DL)-alpha-Lipoic acid"]
CultureMech_000811 --> ing23__DL__alpha_Lipoic_acid
CHEBI_16494["CHEBI:16494"]
ing23__DL__alpha_Lipoic_acid -.-> CHEBI_16494
ing24_ZnSO4_x_7_H2O["ZnSO4 x 7 H2O"]
CultureMech_000811 --> ing24_ZnSO4_x_7_H2O
CHEBI_32312["CHEBI:32312"]
ing24_ZnSO4_x_7_H2O -.-> CHEBI_32312
ing25_MnCl2_x_4_H2O["MnCl2 x 4 H2O"]
CultureMech_000811 --> ing25_MnCl2_x_4_H2O
CHEBI_86368["CHEBI:86368"]
ing25_MnCl2_x_4_H2O -.-> CHEBI_86368
ing26_H3BO3["H3BO3"]
CultureMech_000811 --> ing26_H3BO3
CHEBI_33118["CHEBI:33118"]
ing26_H3BO3 -.-> CHEBI_33118
ing27_CoCl2_x_6_H2O["CoCl2 x 6 H2O"]
CultureMech_000811 --> ing27_CoCl2_x_6_H2O
CHEBI_53503["CHEBI:53503"]
ing27_CoCl2_x_6_H2O -.-> CHEBI_53503
ing28_CuCl2_x_2_H2O["CuCl2 x 2 H2O"]
CultureMech_000811 --> ing28_CuCl2_x_2_H2O
CHEBI_86318["CHEBI:86318"]
ing28_CuCl2_x_2_H2O -.-> CHEBI_86318
ing29_NiCl2_x_6_H2O["NiCl2 x 6 H2O"]
CultureMech_000811 --> ing29_NiCl2_x_6_H2O
CHEBI_34887["CHEBI:34887"]
ing29_NiCl2_x_6_H2O -.-> CHEBI_34887
CultureMech_000811 --> more["...1 more"]
Ingredients (31)
| # | Ingredient | Concentration | Ontology | Notes |
|---|---|---|---|---|
| 1 |
KH2PO4
H2O4P.K 136.084 g/mol
|
0.195886 G_PER_L |
CHEBI:63036
potassium dihydrogen phosphate
|
|
| 2 |
MgCl2 x 6 H2O
2Cl.6H2O.Mg 203.301 g/mol
|
0.0979432 G_PER_L |
CHEBI:86345
magnesium dichloride hexahydrate
|
|
| 3 |
NH4Cl
Cl.H4N 53.492 g/mol
|
0.489716 G_PER_L |
CHEBI:31206
ammonium chloride
|
|
| 4 |
KCl
Cl.K 74.551 g/mol
|
0.195886 G_PER_L |
CHEBI:32588
potassium chloride
|
|
| 5 |
NaCl
Cl.Na 58.443 g/mol
|
58.7659 G_PER_L |
CHEBI:26710
sodium chloride
|
|
| 6 |
Na2CO3
CO3.2Na 105.988 g/mol
|
66.8952 G_PER_L |
CHEBI:29377
sodium carbonate
|
|
| 7 |
NaHCO3
CHO3.Na 84.006 g/mol
|
37.5122 G_PER_L |
CHEBI:32139
sodium hydrogencarbonate
|
|
| 8 |
Resazurin
C12H7NO4 229.191 g/mol
|
0.000489716 G_PER_L |
CHEBI:8806
Resazurin
|
|
| 9 | Yeast extract | 0.0489716 G_PER_L | ||
| 10 |
Ethanol
C2H6O 46.069 g/mol
|
10 G_PER_L |
CHEBI:16236
ethanol
|
|
| 11 |
Na2S x 9 H2O
9H2O.2Na.S 240.182 g/mol
|
0.979432 G_PER_L |
CHEBI:76209
sodium sulfide nonahydrate
|
|
| 12 |
NaNO3
NO3.Na 84.994 g/mol
|
0.832517 G_PER_L |
CHEBI:63005
sodium nitrate
|
|
| 13 |
EDTA
C10H16N2O8 292.244 g/mol
|
0.5 G_PER_L |
CHEBI:4735
ethylenediaminetetraacetic acid
|
|
| 14 |
FeSO4 x 7 H2O
Fe.7H2O.O4S 278.013 g/mol
|
0.2 G_PER_L |
CHEBI:75836
iron(2+) sulfate heptahydrate
|
|
| 15 |
Biotin
C10H16N2O3S 244.316 g/mol
|
0.002 G_PER_L |
CHEBI:15956
biotin
|
|
| 16 |
Folic acid
C19H19N7O6 441.404 g/mol
|
0.002 G_PER_L |
CHEBI:27470
folic acid
|
|
| 17 |
Pyridoxine hydrochloride
C8H11NO3.HCl 205.641 g/mol
|
0.01 G_PER_L |
CHEBI:30961
pyridoxine hydrochloride
|
|
| 18 |
Thiamine HCl
C12H17N4OS.Cl.HCl 337.276 g/mol
|
0.005 G_PER_L |
CHEBI:49105
thiamine hydrochloride
|
|
| 19 |
Riboflavin
C17H20N4O6 376.369 g/mol
|
0.005 G_PER_L |
CHEBI:17015
riboflavin
|
|
| 20 |
Nicotinic acid
C6H5NO2 123.111 g/mol
|
0.005 G_PER_L |
CHEBI:15940
nicotinic acid
|
|
| 21 |
Calcium D-(+)-pantothenate
2C9H16NO5.Ca 476.536 g/mol
|
0.005 G_PER_L |
CHEBI:31345
Calcium pantothenate
|
|
| 22 | Vitamin B12 | 0.0001 G_PER_L |
CHEBI:176843
vitamin B12
|
|
| 23 |
p-Aminobenzoic acid
C7H7NO2 137.138 g/mol
|
0.005 G_PER_L |
CHEBI:30753
4-aminobenzoic acid
|
|
| 24 |
(DL)-alpha-Lipoic acid
C8H14O2S2 206.332 g/mol
|
0.005 G_PER_L |
CHEBI:16494
lipoic acid
|
|
| 25 |
ZnSO4 x 7 H2O
7H2O.O4S.Zn 287.558 g/mol
|
0.1 G_PER_L |
CHEBI:32312
zinc sulfate heptahydrate
|
|
| 26 |
MnCl2 x 4 H2O
Cl2Mn.4H2O 197.904 g/mol
|
0.03 G_PER_L |
CHEBI:86368
manganese(II) chloride tetrahydrate
|
|
| 27 |
H3BO3
H3BO3 61.833 g/mol
|
0.3 G_PER_L |
CHEBI:33118
boric acid
|
|
| 28 |
CoCl2 x 6 H2O
2Cl.Co.6H2O 237.929 g/mol
|
0.2 G_PER_L |
CHEBI:53503
cobalt chloride hexahydrate
|
|
| 29 |
CuCl2 x 2 H2O
Cl2Cu.2H2O 170.482 g/mol
|
0.01 G_PER_L |
CHEBI:86318
copper(II) chloride dihydrate
|
|
| 30 |
NiCl2 x 6 H2O
Cl2Ni 129.599 g/mol
|
0.02 G_PER_L |
CHEBI:34887
nickel dichloride
|
|
| 31 |
Na2MoO4 x 2 H2O
2H2O.MoO4.2Na 241.946 g/mol
|
0.03 G_PER_L |
CHEBI:75213
sodium molybdate dihydrate
|
Preparation
- pH 8.8- 9.5; gas atmosphere: 100% N2.
- Dissolve ingredients except the sodium carbonate, sodium bicarbonate, ethanol, and sodium sulphide. Boil medium for a few minutes and cool to room temperature while flushing with N2. Add and dissolve sodium carbonate, sodium bicarbonate and sodium sulphide while gassing the head space only. Dispense and autoclave under N2. Before use add 10 ml/l (v/v) of 50% (v/v) ethanol solution (flushed and autoclaved under N2).
Target organisms
No target organisms are recorded.
Provenance
| Timestamp | Curator | Action | Notes |
|---|---|---|---|
| 2026-01-26T17:53:14.169377Z | mediadive-import | Imported from MediaDive | Source: DSMZ, ID: 1352 |
| 2026-02-06T05:10:49.855866Z | invalid-chebi-removal | Removed 1 invalid CHEBI ID(s) | Removed CHEBI IDs with invalid format (7+ digits) from MicrobeMediaParam source data |
| 2026-03-13T06:18:58.527325Z | culturemech-id-assigner-v1.0 | Assigned CultureMech ID | Assigned stable identifier: CultureMech:000811 |
| 2026-03-17T06:30:47.000000Z | mediaingredientmech-enrichment-v1.0 | Added MediaIngredientMech links | Linked ingredients to MediaIngredientMech identifiers |
| 2026-03-17T19:45:09.951306+00:00 | normalize_media_names_to_snake_case.py | NAME_NORMALIZED | Normalized name from "FUCHSIELLA ALKALIACETIGENA MEDIUM" to "fuchsiella_alkaliacetigena_medium" for consistent matching |
| 2026-05-20T06:01:20.556441+00:00 | migrate_ph_range.py | MIGRATED_PH_RANGE | ph_range '8.8-9.5' -> {'min': 8.8, 'max': 9.5} |
| 2026-06-05T07:41:05.595272+00:00 | mim-legacy-to-chebi-migration-v1.0 | Refreshed legacy MediaIngredientMech links to CHEBI keying | Replaced 28 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme. |
| 2026-06-10T07:46:58.841759+00:00 | primary-term-label-canonicalization-v1.0 | Canonicalized primary term label to OBO canonical (OAK) | 4 primary term(s); ingredient name resolved by OAK sqlite:obo:chebi. |