Overview
- External ontology
- mediadive.medium:1355 (source link unavailable) — HYPHOMICROBIUM MEDIUM
- Applications
-
- Microbial cultivation
- pH
- 6.0
- Notes
- Source: DSMZ | Link: https://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium1355.pdf
Composition
flowchart LR
CultureMech_000813["hyphomicrobium_medium"]
ing0_K2HPO4["K2HPO4"]
CultureMech_000813 --> ing0_K2HPO4
CHEBI_131527["CHEBI:131527"]
ing0_K2HPO4 -.-> CHEBI_131527
ing1_NaH2PO4["NaH2PO4"]
CultureMech_000813 --> ing1_NaH2PO4
CHEBI_37585["CHEBI:37585"]
ing1_NaH2PO4 -.-> CHEBI_37585
ing2__NH4_2SO4["(NH4)2SO4"]
CultureMech_000813 --> ing2__NH4_2SO4
CHEBI_62946["CHEBI:62946"]
ing2__NH4_2SO4 -.-> CHEBI_62946
ing3_MgSO4_x_7_H2O["MgSO4 x 7 H2O"]
CultureMech_000813 --> ing3_MgSO4_x_7_H2O
CHEBI_31795["CHEBI:31795"]
ing3_MgSO4_x_7_H2O -.-> CHEBI_31795
ing4_Agar["Agar"]
CultureMech_000813 --> ing4_Agar
CHEBI_2509["CHEBI:2509"]
ing4_Agar -.-> CHEBI_2509
ing5_Methylamine["Methylamine"]
CultureMech_000813 --> ing5_Methylamine
CHEBI_16830["CHEBI:16830"]
ing5_Methylamine -.-> CHEBI_16830
ing6_EDTA["EDTA"]
CultureMech_000813 --> ing6_EDTA
CHEBI_4735["CHEBI:4735"]
ing6_EDTA -.-> CHEBI_4735
ing7_ZnSO4_x_7_H2O["ZnSO4 x 7 H2O"]
CultureMech_000813 --> ing7_ZnSO4_x_7_H2O
CHEBI_32312["CHEBI:32312"]
ing7_ZnSO4_x_7_H2O -.-> CHEBI_32312
ing8_CaCl2_x_2_H2O["CaCl2 x 2 H2O"]
CultureMech_000813 --> ing8_CaCl2_x_2_H2O
CHEBI_86158["CHEBI:86158"]
ing8_CaCl2_x_2_H2O -.-> CHEBI_86158
ing9_MnCl2_x_4_H2O["MnCl2 x 4 H2O"]
CultureMech_000813 --> ing9_MnCl2_x_4_H2O
CHEBI_86368["CHEBI:86368"]
ing9_MnCl2_x_4_H2O -.-> CHEBI_86368
ing10_FeSO4_x_7_H2O["FeSO4 x 7 H2O"]
CultureMech_000813 --> ing10_FeSO4_x_7_H2O
CHEBI_75836["CHEBI:75836"]
ing10_FeSO4_x_7_H2O -.-> CHEBI_75836
ing11__NH4_6Mo7O24_x_4_H2O["(NH4)6Mo7O24 x 4 H2O"]
CultureMech_000813 --> ing11__NH4_6Mo7O24_x_4_H2O
CHEBI_91249["CHEBI:91249"]
ing11__NH4_6Mo7O24_x_4_H2O -.-> CHEBI_91249
ing12_CuSO4_x_5_H2O["CuSO4 x 5 H2O"]
CultureMech_000813 --> ing12_CuSO4_x_5_H2O
CHEBI_31440["CHEBI:31440"]
ing12_CuSO4_x_5_H2O -.-> CHEBI_31440
ing13_CoCl2_x_6_H2O["CoCl2 x 6 H2O"]
CultureMech_000813 --> ing13_CoCl2_x_6_H2O
CHEBI_53503["CHEBI:53503"]
ing13_CoCl2_x_6_H2O -.-> CHEBI_53503
Ingredients (14)
| # | Ingredient | Concentration | Ontology | Notes |
|---|---|---|---|---|
| 1 |
K2HPO4
HO4P.2K 174.174 g/mol
|
2.5 G_PER_L |
CHEBI:131527
dipotassium hydrogen phosphate
|
|
| 2 |
NaH2PO4
H2O4P.Na 119.976 g/mol
|
2.25 G_PER_L |
CHEBI:37585
sodium dihydrogenphosphate
|
|
| 3 |
(NH4)2SO4
2H4N.O4S 132.141 g/mol
|
0.5 G_PER_L |
CHEBI:62946
ammonium sulfate
|
|
| 4 |
MgSO4 x 7 H2O
7H2O.Mg.O4S 246.473 g/mol
|
0.2 G_PER_L |
CHEBI:31795
magnesium sulfate heptahydrate
|
|
| 5 | Agar | 12 G_PER_L |
CHEBI:2509
agar
|
(for solid medium) |
| 6 |
Methylamine
CH5N 31.058 g/mol
|
2 G_PER_L |
CHEBI:16830
methylamine
|
|
| 7 |
EDTA
C10H16N2O8 292.244 g/mol
|
10 G_PER_L |
CHEBI:4735
ethylenediaminetetraacetic acid
|
|
| 8 |
ZnSO4 x 7 H2O
7H2O.O4S.Zn 287.558 g/mol
|
4.4 G_PER_L |
CHEBI:32312
zinc sulfate heptahydrate
|
|
| 9 |
CaCl2 x 2 H2O
Ca.2Cl.2H2O 147.014 g/mol
|
1.466 G_PER_L |
CHEBI:86158
calcium chloride dihydrate
|
|
| 10 |
MnCl2 x 4 H2O
Cl2Mn.4H2O 197.904 g/mol
|
1.012 G_PER_L |
CHEBI:86368
manganese(II) chloride tetrahydrate
|
|
| 11 |
FeSO4 x 7 H2O
Fe.7H2O.O4S 278.013 g/mol
|
0.998 G_PER_L |
CHEBI:75836
iron(2+) sulfate heptahydrate
|
|
| 12 |
(NH4)6Mo7O24 x 4 H2O
2H4N.MoO4 196.014 g/mol
|
0.22 G_PER_L |
CHEBI:91249
ammonium molybdate
|
|
| 13 |
CuSO4 x 5 H2O
Cu.5H2O.O4S 249.684 g/mol
|
0.314 G_PER_L |
CHEBI:31440
copper(II) sulfate pentahydrate
|
|
| 14 |
CoCl2 x 6 H2O
2Cl.Co.6H2O 237.929 g/mol
|
0.322 G_PER_L |
CHEBI:53503
cobalt chloride hexahydrate
|
Preparation
- Adjust to pH 6 and sterilize by autoclaving. The medium may be solidified by adding 12.0 g/l agar. After autoclaving add sterile methylamine (0.2% v/v) to the sterile main solution.
- Adjust pH to 6.0 with NaOH.
Target organisms
No target organisms are recorded.
Provenance
| Timestamp | Curator | Action | Notes |
|---|---|---|---|
| 2026-01-26T17:53:14.180315Z | mediadive-import | Imported from MediaDive | Source: DSMZ, ID: 1355 |
| 2026-03-13T06:18:58.547274Z | culturemech-id-assigner-v1.0 | Assigned CultureMech ID | Assigned stable identifier: CultureMech:000813 |
| 2026-03-17T06:30:27.000000Z | mediaingredientmech-enrichment-v1.0 | Added MediaIngredientMech links | Linked ingredients to MediaIngredientMech identifiers |
| 2026-03-17T19:45:09.973767+00:00 | normalize_media_names_to_snake_case.py | NAME_NORMALIZED | Normalized name from "HYPHOMICROBIUM MEDIUM" to "hyphomicrobium_medium" for consistent matching |
| 2026-06-05T07:41:09.198231+00:00 | mim-legacy-to-chebi-migration-v1.0 | Refreshed legacy MediaIngredientMech links to CHEBI keying | Replaced 13 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme. |
| 2026-06-10T07:46:59.984874+00:00 | primary-term-label-canonicalization-v1.0 | Canonicalized primary term label to OBO canonical (OAK) | 3 primary term(s); ingredient name resolved by OAK sqlite:obo:chebi. |