Overview
- External ontology
- mediadive.medium:1357 (source link unavailable) — MAB MEDIUM
- Applications
-
- Microbial cultivation
- pH
- 9.8
- Notes
- Source: DSMZ | Link: https://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium1357.pdf
Composition
flowchart LR
CultureMech_000815["mab_medium"]
ing0_NaCl["NaCl"]
CultureMech_000815 --> ing0_NaCl
CHEBI_26710["CHEBI:26710"]
ing0_NaCl -.-> CHEBI_26710
ing1_NH4Cl["NH4Cl"]
CultureMech_000815 --> ing1_NH4Cl
CHEBI_31206["CHEBI:31206"]
ing1_NH4Cl -.-> CHEBI_31206
ing2_K2HPO4["K2HPO4"]
CultureMech_000815 --> ing2_K2HPO4
CHEBI_131527["CHEBI:131527"]
ing2_K2HPO4 -.-> CHEBI_131527
ing3_MgSO4_x_7_H2O["MgSO4 x 7 H2O"]
CultureMech_000815 --> ing3_MgSO4_x_7_H2O
CHEBI_31795["CHEBI:31795"]
ing3_MgSO4_x_7_H2O -.-> CHEBI_31795
ing4_CaCl2_x_2_H2O["CaCl2 x 2 H2O"]
CultureMech_000815 --> ing4_CaCl2_x_2_H2O
CHEBI_86158["CHEBI:86158"]
ing4_CaCl2_x_2_H2O -.-> CHEBI_86158
ing5_FeSO4_x_7_H2O["FeSO4 x 7 H2O"]
CultureMech_000815 --> ing5_FeSO4_x_7_H2O
CHEBI_75836["CHEBI:75836"]
ing5_FeSO4_x_7_H2O -.-> CHEBI_75836
ing6_MnSO4_x_H2O["MnSO4 x H2O"]
CultureMech_000815 --> ing6_MnSO4_x_H2O
CHEBI_86364["CHEBI:86364"]
ing6_MnSO4_x_H2O -.-> CHEBI_86364
ing7_CoCl2_x_6_H2O["CoCl2 x 6 H2O"]
CultureMech_000815 --> ing7_CoCl2_x_6_H2O
CHEBI_53503["CHEBI:53503"]
ing7_CoCl2_x_6_H2O -.-> CHEBI_53503
ing8_ZnSO4_x_7_H2O["ZnSO4 x 7 H2O"]
CultureMech_000815 --> ing8_ZnSO4_x_7_H2O
CHEBI_32312["CHEBI:32312"]
ing8_ZnSO4_x_7_H2O -.-> CHEBI_32312
ing9_Resazurin["Resazurin"]
CultureMech_000815 --> ing9_Resazurin
CHEBI_8806["CHEBI:8806"]
ing9_Resazurin -.-> CHEBI_8806
ing10_Na2CO3["Na2CO3"]
CultureMech_000815 --> ing10_Na2CO3
CHEBI_29377["CHEBI:29377"]
ing10_Na2CO3 -.-> CHEBI_29377
ing11_NaHCO3["NaHCO3"]
CultureMech_000815 --> ing11_NaHCO3
CHEBI_32139["CHEBI:32139"]
ing11_NaHCO3 -.-> CHEBI_32139
ing12_Cysteine_HCl["Cysteine HCl"]
CultureMech_000815 --> ing12_Cysteine_HCl
ing13_CuSO4_x_2_H2O["CuSO4 x 2 H2O"]
CultureMech_000815 --> ing13_CuSO4_x_2_H2O
CHEBI_23414["CHEBI:23414"]
ing13_CuSO4_x_2_H2O -.-> CHEBI_23414
ing14_Nitrilotriacetic_acid["Nitrilotriacetic acid"]
CultureMech_000815 --> ing14_Nitrilotriacetic_acid
CHEBI_44557["CHEBI:44557"]
ing14_Nitrilotriacetic_acid -.-> CHEBI_44557
ing15_AlK_SO4_2_x_12_H2O["AlK(SO4)2 x 12 H2O"]
CultureMech_000815 --> ing15_AlK_SO4_2_x_12_H2O
CHEBI_86465["CHEBI:86465"]
ing15_AlK_SO4_2_x_12_H2O -.-> CHEBI_86465
ing16_H3BO3["H3BO3"]
CultureMech_000815 --> ing16_H3BO3
CHEBI_33118["CHEBI:33118"]
ing16_H3BO3 -.-> CHEBI_33118
ing17_Na2MoO4_x_2_H2O["Na2MoO4 x 2 H2O"]
CultureMech_000815 --> ing17_Na2MoO4_x_2_H2O
CHEBI_75213["CHEBI:75213"]
ing17_Na2MoO4_x_2_H2O -.-> CHEBI_75213
ing18_Pyridoxine_hydrochloride["Pyridoxine hydrochloride"]
CultureMech_000815 --> ing18_Pyridoxine_hydrochloride
CHEBI_30961["CHEBI:30961"]
ing18_Pyridoxine_hydrochloride -.-> CHEBI_30961
ing19_Thiamine_HCl["Thiamine HCl"]
CultureMech_000815 --> ing19_Thiamine_HCl
CHEBI_49105["CHEBI:49105"]
ing19_Thiamine_HCl -.-> CHEBI_49105
ing20_Riboflavin["Riboflavin"]
CultureMech_000815 --> ing20_Riboflavin
CHEBI_17015["CHEBI:17015"]
ing20_Riboflavin -.-> CHEBI_17015
ing21_Nicotinic_acid["Nicotinic acid"]
CultureMech_000815 --> ing21_Nicotinic_acid
CHEBI_15940["CHEBI:15940"]
ing21_Nicotinic_acid -.-> CHEBI_15940
ing22_p_Aminobenzoic_acid["p-Aminobenzoic acid"]
CultureMech_000815 --> ing22_p_Aminobenzoic_acid
CHEBI_30753["CHEBI:30753"]
ing22_p_Aminobenzoic_acid -.-> CHEBI_30753
ing23_Lipoic_acid["Lipoic acid"]
CultureMech_000815 --> ing23_Lipoic_acid
CHEBI_16494["CHEBI:16494"]
ing23_Lipoic_acid -.-> CHEBI_16494
ing24_Biotin["Biotin"]
CultureMech_000815 --> ing24_Biotin
CHEBI_15956["CHEBI:15956"]
ing24_Biotin -.-> CHEBI_15956
ing25_Folic_acid["Folic acid"]
CultureMech_000815 --> ing25_Folic_acid
CHEBI_27470["CHEBI:27470"]
ing25_Folic_acid -.-> CHEBI_27470
ing26_Vitamin_B12["Vitamin B12"]
CultureMech_000815 --> ing26_Vitamin_B12
CHEBI_176843["CHEBI:176843"]
ing26_Vitamin_B12 -.-> CHEBI_176843
Ingredients (27)
| # | Ingredient | Concentration | Ontology | Notes |
|---|---|---|---|---|
| 1 |
NaCl
Cl.Na 58.443 g/mol
|
10 G_PER_L |
CHEBI:26710
sodium chloride
|
|
| 2 |
NH4Cl
Cl.H4N 53.492 g/mol
|
0.85 G_PER_L |
CHEBI:31206
ammonium chloride
|
|
| 3 |
K2HPO4
HO4P.2K 174.174 g/mol
|
0.48 G_PER_L |
CHEBI:131527
dipotassium hydrogen phosphate
|
|
| 4 |
MgSO4 x 7 H2O
7H2O.Mg.O4S 246.473 g/mol
|
0.12 G_PER_L |
CHEBI:31795
magnesium sulfate heptahydrate
|
|
| 5 |
CaCl2 x 2 H2O
Ca.2Cl.2H2O 147.014 g/mol
|
0.061 G_PER_L |
CHEBI:86158
calcium chloride dihydrate
|
|
| 6 |
FeSO4 x 7 H2O
Fe.7H2O.O4S 278.013 g/mol
|
0.021 G_PER_L |
CHEBI:75836
iron(2+) sulfate heptahydrate
|
|
| 7 |
MnSO4 x H2O
H2O.Mn.O4S 169.016 g/mol
|
0.005 G_PER_L |
CHEBI:86364
manganese(II) sulfate monohydrate
|
|
| 8 |
CoCl2 x 6 H2O
2Cl.Co.6H2O 237.929 g/mol
|
0.001 G_PER_L |
CHEBI:53503
cobalt chloride hexahydrate
|
|
| 9 |
ZnSO4 x 7 H2O
7H2O.O4S.Zn 287.558 g/mol
|
0.001 G_PER_L |
CHEBI:32312
zinc sulfate heptahydrate
|
|
| 10 |
Resazurin
C12H7NO4 229.191 g/mol
|
1e-06 G_PER_L |
CHEBI:8806
Resazurin
|
|
| 11 |
Na2CO3
CO3.2Na 105.988 g/mol
|
10.6 G_PER_L |
CHEBI:29377
sodium carbonate
|
|
| 12 |
NaHCO3
CHO3.Na 84.006 g/mol
|
13.6 G_PER_L |
CHEBI:32139
sodium hydrogencarbonate
|
|
| 13 | Cysteine HCl | 0.7 G_PER_L | ||
| 14 |
CuSO4 x 2 H2O
Cu.O4S 159.609 g/mol
|
0.1 G_PER_L |
CHEBI:23414
copper(II) sulfate
|
|
| 15 |
Nitrilotriacetic acid
C6H9NO6 191.139 g/mol
|
15 G_PER_L |
CHEBI:44557
nitrilotriacetic acid
|
|
| 16 |
AlK(SO4)2 x 12 H2O
Al.12H2O.K.2O4S 474.386 g/mol
|
0.1 G_PER_L |
CHEBI:86465
potassium aluminium sulfate dodecahydrate
|
|
| 17 |
H3BO3
H3BO3 61.833 g/mol
|
0.1 G_PER_L |
CHEBI:33118
boric acid
|
|
| 18 |
Na2MoO4 x 2 H2O
2H2O.MoO4.2Na 241.946 g/mol
|
0.1 G_PER_L |
CHEBI:75213
sodium molybdate dihydrate
|
|
| 19 |
Pyridoxine hydrochloride
C8H11NO3.HCl 205.641 g/mol
|
0.0001 G_PER_L |
CHEBI:30961
pyridoxine hydrochloride
|
|
| 20 |
Thiamine HCl
C12H17N4OS.Cl.HCl 337.276 g/mol
|
5e-05 G_PER_L |
CHEBI:49105
thiamine hydrochloride
|
|
| 21 |
Riboflavin
C17H20N4O6 376.369 g/mol
|
5e-05 G_PER_L |
CHEBI:17015
riboflavin
|
|
| 22 |
Nicotinic acid
C6H5NO2 123.111 g/mol
|
5e-05 G_PER_L |
CHEBI:15940
nicotinic acid
|
|
| 23 |
p-Aminobenzoic acid
C7H7NO2 137.138 g/mol
|
5e-05 G_PER_L |
CHEBI:30753
4-aminobenzoic acid
|
|
| 24 |
Lipoic acid
C8H14O2S2 206.332 g/mol
|
5e-05 G_PER_L |
CHEBI:16494
lipoic acid
|
|
| 25 |
Biotin
C10H16N2O3S 244.316 g/mol
|
2e-05 G_PER_L |
CHEBI:15956
biotin
|
|
| 26 |
Folic acid
C19H19N7O6 441.404 g/mol
|
2e-05 G_PER_L |
CHEBI:27470
folic acid
|
|
| 27 | Vitamin B12 | 5e-06 G_PER_L |
CHEBI:176843
vitamin B12
|
Preparation
- Prepare the liquid medium without Na2CO3, NaHCO3, Vitamin solution and cysteine HCl. Boil and then cool under N2 to room temperature. Add Na2CO3 and NaHCO3. Dispense into suitable vessels for anaerobic growth (serum bottles or Hungate tubes sealed with rubber stoppers) and sterilize by autoclaving. After autoclaving add cysteine HCl from sterile stock solution and 10 ml of the vitamin solution to 1 litre of the sterile main solution. Final pH 9.8.
Target organisms
No target organisms are recorded.
Provenance
| Timestamp | Curator | Action | Notes |
|---|---|---|---|
| 2026-01-26T17:53:14.184729Z | mediadive-import | Imported from MediaDive | Source: DSMZ, ID: 1357 |
| 2026-03-13T06:18:58.563071Z | culturemech-id-assigner-v1.0 | Assigned CultureMech ID | Assigned stable identifier: CultureMech:000815 |
| 2026-03-17T06:30:35.000000Z | mediaingredientmech-enrichment-v1.0 | Added MediaIngredientMech links | Linked ingredients to MediaIngredientMech identifiers |
| 2026-03-17T19:45:09.991393+00:00 | normalize_media_names_to_snake_case.py | NAME_NORMALIZED | Normalized name from "MAB MEDIUM" to "mab_medium" for consistent matching |
| 2026-06-05T07:41:12.449242+00:00 | mim-legacy-to-chebi-migration-v1.0 | Refreshed legacy MediaIngredientMech links to CHEBI keying | Replaced 26 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme. |
| 2026-06-10T07:47:00.765796+00:00 | primary-term-label-canonicalization-v1.0 | Canonicalized primary term label to OBO canonical (OAK) | 5 primary term(s); ingredient name resolved by OAK sqlite:obo:chebi. |