phaeovibrio_sulphidiphilus_medium

CultureMech:000817 bacterial COMPLEX LIQUID
Original name: PHAEOVIBRIO SULPHIDIPHILUS MEDIUM

Overview

External ontology
mediadive.medium:1359 (source link unavailable) — PHAEOVIBRIO SULPHIDIPHILUS MEDIUM
Applications
  • Microbial cultivation
pH range
{'min': 6.6, 'max': 7.4}
Notes
Source: DSMZ | Link: https://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium1359.pdf

Composition

flowchart LR
    CultureMech_000817["phaeovibrio_sulphidiphilus_medium"]
    ing0_KH2PO4["KH2PO4"]
    CultureMech_000817 --> ing0_KH2PO4
    CHEBI_63036["CHEBI:63036"]
    ing0_KH2PO4 -.-> CHEBI_63036
    ing1_MgSO4_x_7_H2O["MgSO4 x 7 H2O"]
    CultureMech_000817 --> ing1_MgSO4_x_7_H2O
    CHEBI_31795["CHEBI:31795"]
    ing1_MgSO4_x_7_H2O -.-> CHEBI_31795
    ing2_NaCl["NaCl"]
    CultureMech_000817 --> ing2_NaCl
    CHEBI_26710["CHEBI:26710"]
    ing2_NaCl -.-> CHEBI_26710
    ing3_NH4Cl["NH4Cl"]
    CultureMech_000817 --> ing3_NH4Cl
    CHEBI_31206["CHEBI:31206"]
    ing3_NH4Cl -.-> CHEBI_31206
    ing4_CaCl2_x_2_H2O["CaCl2 x 2 H2O"]
    CultureMech_000817 --> ing4_CaCl2_x_2_H2O
    CHEBI_86158["CHEBI:86158"]
    ing4_CaCl2_x_2_H2O -.-> CHEBI_86158
    ing5_Na_pyruvate["Na pyruvate"]
    CultureMech_000817 --> ing5_Na_pyruvate
    CHEBI_50144["CHEBI:50144"]
    ing5_Na_pyruvate -.-> CHEBI_50144
    ing6_Sodium_succinate["Sodium succinate"]
    CultureMech_000817 --> ing6_Sodium_succinate
    CHEBI_63675["CHEBI:63675"]
    ing6_Sodium_succinate -.-> CHEBI_63675
    ing7_Na_acetate["Na acetate"]
    CultureMech_000817 --> ing7_Na_acetate
    CHEBI_32954["CHEBI:32954"]
    ing7_Na_acetate -.-> CHEBI_32954
    ing8_Yeast_extract["Yeast extract"]
    CultureMech_000817 --> ing8_Yeast_extract
    ing9_Ferric_citrate["Ferric citrate"]
    CultureMech_000817 --> ing9_Ferric_citrate
    CHEBI_144421["CHEBI:144421"]
    ing9_Ferric_citrate -.-> CHEBI_144421
    ing10_NaHCO3["NaHCO3"]
    CultureMech_000817 --> ing10_NaHCO3
    CHEBI_32139["CHEBI:32139"]
    ing10_NaHCO3 -.-> CHEBI_32139
    ing11_Na2S_x_9_H2O["Na2S x 9 H2O"]
    CultureMech_000817 --> ing11_Na2S_x_9_H2O
    CHEBI_76209["CHEBI:76209"]
    ing11_Na2S_x_9_H2O -.-> CHEBI_76209
    ing12_Vitamin_B12["Vitamin B12"]
    CultureMech_000817 --> ing12_Vitamin_B12
    CHEBI_176843["CHEBI:176843"]
    ing12_Vitamin_B12 -.-> CHEBI_176843
    ing13_HCl["HCl"]
    CultureMech_000817 --> ing13_HCl
    CHEBI_17883["CHEBI:17883"]
    ing13_HCl -.-> CHEBI_17883
    ing14_ZnCl2["ZnCl2"]
    CultureMech_000817 --> ing14_ZnCl2
    CHEBI_49976["CHEBI:49976"]
    ing14_ZnCl2 -.-> CHEBI_49976
    ing15_MnCl2_x_4_H2O["MnCl2 x 4 H2O"]
    CultureMech_000817 --> ing15_MnCl2_x_4_H2O
    CHEBI_86368["CHEBI:86368"]
    ing15_MnCl2_x_4_H2O -.-> CHEBI_86368
    ing16_H3BO3["H3BO3"]
    CultureMech_000817 --> ing16_H3BO3
    CHEBI_33118["CHEBI:33118"]
    ing16_H3BO3 -.-> CHEBI_33118
    ing17_CoCl2_x_6_H2O["CoCl2 x 6 H2O"]
    CultureMech_000817 --> ing17_CoCl2_x_6_H2O
    CHEBI_53503["CHEBI:53503"]
    ing17_CoCl2_x_6_H2O -.-> CHEBI_53503
    ing18_CuCl2_x_2_H2O["CuCl2 x 2 H2O"]
    CultureMech_000817 --> ing18_CuCl2_x_2_H2O
    CHEBI_86318["CHEBI:86318"]
    ing18_CuCl2_x_2_H2O -.-> CHEBI_86318
    ing19_NiCl2_x_6_H2O["NiCl2 x 6 H2O"]
    CultureMech_000817 --> ing19_NiCl2_x_6_H2O
    CHEBI_34887["CHEBI:34887"]
    ing19_NiCl2_x_6_H2O -.-> CHEBI_34887
    ing20_Na2MoO4_x_2_H2O["Na2MoO4 x 2 H2O"]
    CultureMech_000817 --> ing20_Na2MoO4_x_2_H2O
    CHEBI_75213["CHEBI:75213"]
    ing20_Na2MoO4_x_2_H2O -.-> CHEBI_75213

Ingredients (21)

# Ingredient Concentration Ontology Notes
1 KH2PO4
H2O4P.K 136.084 g/mol
0.5 G_PER_L CHEBI:63036
potassium dihydrogen phosphate
2 MgSO4 x 7 H2O
7H2O.Mg.O4S 246.473 g/mol
0.5 G_PER_L CHEBI:31795
magnesium sulfate heptahydrate
3 NaCl
Cl.Na 58.443 g/mol
0.4 G_PER_L CHEBI:26710
sodium chloride
4 NH4Cl
Cl.H4N 53.492 g/mol
0.68 G_PER_L CHEBI:31206
ammonium chloride
5 CaCl2 x 2 H2O
Ca.2Cl.2H2O 147.014 g/mol
0.25 G_PER_L CHEBI:86158
calcium chloride dihydrate
6 Na pyruvate
C3H3O3.Na 110.044 g/mol
0.5 G_PER_L CHEBI:50144
sodium pyruvate
7 Sodium succinate
C4H4O4.2Na 162.052 g/mol
0.5 G_PER_L CHEBI:63675
sodium succinate (anhydrous)
8 Na acetate
C2H3O2.Na 82.034 g/mol
0.5 G_PER_L CHEBI:32954
sodium acetate
9 Yeast extract 0.5 G_PER_L
10 Ferric citrate
C6H5FeO7 244.944 g/mol
0.005 G_PER_L CHEBI:144421
iron(III) citrate
11 NaHCO3
CHO3.Na 84.006 g/mol
1.5 G_PER_L CHEBI:32139
sodium hydrogencarbonate
12 Na2S x 9 H2O
9H2O.2Na.S 240.182 g/mol
0.240182 G_PER_L CHEBI:76209
sodium sulfide nonahydrate
13 Vitamin B12 1 G_PER_L CHEBI:176843
vitamin B12
14 HCl
HCl 36.461 g/mol
1 G_PER_L CHEBI:17883
hydrogen chloride
15 ZnCl2
2Cl.Zn 136.296 g/mol
0.0699301 G_PER_L CHEBI:49976
zinc dichloride
16 MnCl2 x 4 H2O
Cl2Mn.4H2O 197.904 g/mol
0.0999001 G_PER_L CHEBI:86368
manganese(II) chloride tetrahydrate
17 H3BO3
H3BO3 61.833 g/mol
0.0599401 G_PER_L CHEBI:33118
boric acid
18 CoCl2 x 6 H2O
2Cl.Co.6H2O 237.929 g/mol
0.1998 G_PER_L CHEBI:53503
cobalt chloride hexahydrate
19 CuCl2 x 2 H2O
Cl2Cu.2H2O 170.482 g/mol
0.01998 G_PER_L CHEBI:86318
copper(II) chloride dihydrate
20 NiCl2 x 6 H2O
Cl2Ni 129.599 g/mol
0.01998 G_PER_L CHEBI:34887
nickel dichloride
21 Na2MoO4 x 2 H2O
2H2O.MoO4.2Na 241.946 g/mol
0.03996 G_PER_L CHEBI:75213
sodium molybdate dihydrate

Preparation

  1. Prepare the basal medium under nitrogen.
  2. NaHCO3 solution (autoclaved in sealed, half full vessels under CO2), Na2S x 9H2O solution (autoclaved) and vitamin B12 solution (filter sterilised) is added to the autoclaved medium and the final pH adjusted to 6.6 - 7.4.

Target organisms

No target organisms are recorded.

Provenance

TimestampCuratorActionNotes
2026-01-26T17:53:14.192377Z mediadive-import Imported from MediaDive Source: DSMZ, ID: 1359
2026-03-13T06:18:58.583098Z culturemech-id-assigner-v1.0 Assigned CultureMech ID Assigned stable identifier: CultureMech:000817
2026-03-17T06:30:41.000000Z mediaingredientmech-enrichment-v1.0 Added MediaIngredientMech links Linked ingredients to MediaIngredientMech identifiers
2026-03-17T19:45:10.015018+00:00 normalize_media_names_to_snake_case.py NAME_NORMALIZED Normalized name from "PHAEOVIBRIO SULPHIDIPHILUS MEDIUM" to "phaeovibrio_sulphidiphilus_medium" for consistent matching
2026-05-20T06:02:02.406986+00:00 migrate_ph_range.py MIGRATED_PH_RANGE ph_range '6.6-7.4' -> {'min': 6.6, 'max': 7.4}
2026-06-05T07:41:41.697079+00:00 mim-legacy-to-chebi-migration-v1.0 Refreshed legacy MediaIngredientMech links to CHEBI keying Replaced 20 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme.
2026-06-10T07:47:10.162918+00:00 primary-term-label-canonicalization-v1.0 Canonicalized primary term label to OBO canonical (OAK) 5 primary term(s); ingredient name resolved by OAK sqlite:obo:chebi.