hippea_jasoniae_medium

CultureMech:000821 bacterial COMPLEX LIQUID
Original name: HIPPEA JASONIAE MEDIUM

Overview

External ontology
mediadive.medium:1360 (source link unavailable) — HIPPEA JASONIAE MEDIUM
Applications
  • Microbial cultivation
pH
4.5
Notes
Source: DSMZ | Link: https://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium1360.pdf

Composition

flowchart LR
    CultureMech_000821["hippea_jasoniae_medium"]
    ing0_NaCl["NaCl"]
    CultureMech_000821 --> ing0_NaCl
    CHEBI_26710["CHEBI:26710"]
    ing0_NaCl -.-> CHEBI_26710
    ing1_MgCl2_x_6_H2O["MgCl2 x 6 H2O"]
    CultureMech_000821 --> ing1_MgCl2_x_6_H2O
    CHEBI_86345["CHEBI:86345"]
    ing1_MgCl2_x_6_H2O -.-> CHEBI_86345
    ing2_KCl["KCl"]
    CultureMech_000821 --> ing2_KCl
    CHEBI_32588["CHEBI:32588"]
    ing2_KCl -.-> CHEBI_32588
    ing3__NH4_2SO4["(NH4)2SO4"]
    CultureMech_000821 --> ing3__NH4_2SO4
    CHEBI_62946["CHEBI:62946"]
    ing3__NH4_2SO4 -.-> CHEBI_62946
    ing4_KH2PO4["KH2PO4"]
    CultureMech_000821 --> ing4_KH2PO4
    CHEBI_63036["CHEBI:63036"]
    ing4_KH2PO4 -.-> CHEBI_63036
    ing5_MgSO4_x_7_H2O["MgSO4 x 7 H2O"]
    CultureMech_000821 --> ing5_MgSO4_x_7_H2O
    CHEBI_31795["CHEBI:31795"]
    ing5_MgSO4_x_7_H2O -.-> CHEBI_31795
    ing6_CaCl2_x_2_H2O["CaCl2 x 2 H2O"]
    CultureMech_000821 --> ing6_CaCl2_x_2_H2O
    CHEBI_86158["CHEBI:86158"]
    ing6_CaCl2_x_2_H2O -.-> CHEBI_86158
    ing7_FeCl3_x_6_H2O["FeCl3 x 6 H2O"]
    CultureMech_000821 --> ing7_FeCl3_x_6_H2O
    CHEBI_86254["CHEBI:86254"]
    ing7_FeCl3_x_6_H2O -.-> CHEBI_86254
    ing8_Na_acetate["Na-acetate"]
    CultureMech_000821 --> ing8_Na_acetate
    CHEBI_32954["CHEBI:32954"]
    ing8_Na_acetate -.-> CHEBI_32954
    ing9_Trisodium_citrate_x_2_H2O["Trisodium citrate x 2 H2O"]
    CultureMech_000821 --> ing9_Trisodium_citrate_x_2_H2O
    CHEBI_53258["CHEBI:53258"]
    ing9_Trisodium_citrate_x_2_H2O -.-> CHEBI_53258
    ing10_Sodium_resazurin["Sodium resazurin"]
    CultureMech_000821 --> ing10_Sodium_resazurin
    CHEBI_8806["CHEBI:8806"]
    ing10_Sodium_resazurin -.-> CHEBI_8806
    ing11_Sulfur["Sulfur"]
    CultureMech_000821 --> ing11_Sulfur
    CHEBI_26833["CHEBI:26833"]
    ing11_Sulfur -.-> CHEBI_26833
    ing12_Yeast_extract["Yeast extract"]
    CultureMech_000821 --> ing12_Yeast_extract
    ing13_Na2S_x_9_H2O["Na2S x 9 H2O"]
    CultureMech_000821 --> ing13_Na2S_x_9_H2O
    CHEBI_76209["CHEBI:76209"]
    ing13_Na2S_x_9_H2O -.-> CHEBI_76209
    ing14_MnCl2_x_4_H2O["MnCl2 x 4 H2O"]
    CultureMech_000821 --> ing14_MnCl2_x_4_H2O
    CHEBI_86368["CHEBI:86368"]
    ing14_MnCl2_x_4_H2O -.-> CHEBI_86368
    ing15_Na2B4O7_x_10_H2O["Na2B4O7 x 10 H2O"]
    CultureMech_000821 --> ing15_Na2B4O7_x_10_H2O
    CHEBI_131366["CHEBI:131366"]
    ing15_Na2B4O7_x_10_H2O -.-> CHEBI_131366
    ing16_ZnSO4_x_7_H2O["ZnSO4 x 7 H2O"]
    CultureMech_000821 --> ing16_ZnSO4_x_7_H2O
    CHEBI_32312["CHEBI:32312"]
    ing16_ZnSO4_x_7_H2O -.-> CHEBI_32312
    ing17_CuCl2_x_2_H2O["CuCl2 x 2 H2O"]
    CultureMech_000821 --> ing17_CuCl2_x_2_H2O
    CHEBI_86318["CHEBI:86318"]
    ing17_CuCl2_x_2_H2O -.-> CHEBI_86318
    ing18_Na2MoO4_x_2_H2O["Na2MoO4 x 2 H2O"]
    CultureMech_000821 --> ing18_Na2MoO4_x_2_H2O
    CHEBI_75213["CHEBI:75213"]
    ing18_Na2MoO4_x_2_H2O -.-> CHEBI_75213
    ing19_VOSO4_x_2_H2O["VOSO4 x 2 H2O"]
    CultureMech_000821 --> ing19_VOSO4_x_2_H2O
    CHEBI_87009["CHEBI:87009"]
    ing19_VOSO4_x_2_H2O -.-> CHEBI_87009
    ing20_CoSO4_x_7_H2O["CoSO4 x 7 H2O"]
    CultureMech_000821 --> ing20_CoSO4_x_7_H2O
    CHEBI_91244["CHEBI:91244"]
    ing20_CoSO4_x_7_H2O -.-> CHEBI_91244
    ing21_Biotin["Biotin"]
    CultureMech_000821 --> ing21_Biotin
    CHEBI_15956["CHEBI:15956"]
    ing21_Biotin -.-> CHEBI_15956
    ing22_Folic_acid["Folic acid"]
    CultureMech_000821 --> ing22_Folic_acid
    CHEBI_27470["CHEBI:27470"]
    ing22_Folic_acid -.-> CHEBI_27470
    ing23_Pyridoxine_hydrochloride["Pyridoxine hydrochloride"]
    CultureMech_000821 --> ing23_Pyridoxine_hydrochloride
    CHEBI_30961["CHEBI:30961"]
    ing23_Pyridoxine_hydrochloride -.-> CHEBI_30961
    ing24_Thiamine_HCl["Thiamine HCl"]
    CultureMech_000821 --> ing24_Thiamine_HCl
    CHEBI_49105["CHEBI:49105"]
    ing24_Thiamine_HCl -.-> CHEBI_49105
    ing25_Riboflavin["Riboflavin"]
    CultureMech_000821 --> ing25_Riboflavin
    CHEBI_17015["CHEBI:17015"]
    ing25_Riboflavin -.-> CHEBI_17015
    ing26_Nicotinic_acid["Nicotinic acid"]
    CultureMech_000821 --> ing26_Nicotinic_acid
    CHEBI_15940["CHEBI:15940"]
    ing26_Nicotinic_acid -.-> CHEBI_15940
    ing27_Calcium_D_____pantothenate["Calcium D-(+)-pantothenate"]
    CultureMech_000821 --> ing27_Calcium_D_____pantothenate
    CHEBI_31345["CHEBI:31345"]
    ing27_Calcium_D_____pantothenate -.-> CHEBI_31345
    ing28_Vitamin_B12["Vitamin B12"]
    CultureMech_000821 --> ing28_Vitamin_B12
    CHEBI_176843["CHEBI:176843"]
    ing28_Vitamin_B12 -.-> CHEBI_176843
    ing29_p_Aminobenzoic_acid["p-Aminobenzoic acid"]
    CultureMech_000821 --> ing29_p_Aminobenzoic_acid
    CHEBI_30753["CHEBI:30753"]
    ing29_p_Aminobenzoic_acid -.-> CHEBI_30753
    CultureMech_000821 --> more["...1 more"]

Ingredients (31)

# Ingredient Concentration Ontology Notes
1 NaCl
Cl.Na 58.443 g/mol
26.0435 G_PER_L CHEBI:26710
sodium chloride
2 MgCl2 x 6 H2O
2Cl.6H2O.Mg 203.301 g/mol
1.48368 G_PER_L CHEBI:86345
magnesium dichloride hexahydrate
3 KCl
Cl.K 74.551 g/mol
0.445104 G_PER_L CHEBI:32588
potassium chloride
4 (NH4)2SO4
2H4N.O4S 132.141 g/mol
1.28586 G_PER_L CHEBI:62946
ammonium sulfate
5 KH2PO4
H2O4P.K 136.084 g/mol
0.276954 G_PER_L CHEBI:63036
potassium dihydrogen phosphate
6 MgSO4 x 7 H2O
7H2O.Mg.O4S 246.473 g/mol
0.24728 G_PER_L CHEBI:31795
magnesium sulfate heptahydrate
7 CaCl2 x 2 H2O
Ca.2Cl.2H2O 147.014 g/mol
0.0692384 G_PER_L CHEBI:86158
calcium chloride dihydrate
8 FeCl3 x 6 H2O
Cl3Fe.6H2O 270.294 g/mol
0.0197824 G_PER_L CHEBI:86254
iron trichloride hexahydrate
9 Na-acetate
C2H3O2.Na 82.034 g/mol
1.2364 G_PER_L CHEBI:32954
sodium acetate
10 Trisodium citrate x 2 H2O
C6H5O7.3Na 258.069 g/mol
2.90801 G_PER_L CHEBI:53258
sodium citrate
11 Sodium resazurin
C12H7NO4 229.191 g/mol
0.00049456 G_PER_L CHEBI:8806
Resazurin
12 Sulfur
S 32.067 g/mol
9.8912 G_PER_L CHEBI:26833
sulfur atom
 (powdered)
13 Yeast extract 0.49456 G_PER_L
14 Na2S x 9 H2O
9H2O.2Na.S 240.182 g/mol
0.49456 G_PER_L CHEBI:76209
sodium sulfide nonahydrate
15 MnCl2 x 4 H2O
Cl2Mn.4H2O 197.904 g/mol
0.18 G_PER_L CHEBI:86368
manganese(II) chloride tetrahydrate
16 Na2B4O7 x 10 H2O
10H2O.H4B4O9.2Na 417.401 g/mol
0.45 G_PER_L CHEBI:131366
disodium tetraborate decahydrate
17 ZnSO4 x 7 H2O
7H2O.O4S.Zn 287.558 g/mol
0.022 G_PER_L CHEBI:32312
zinc sulfate heptahydrate
18 CuCl2 x 2 H2O
Cl2Cu.2H2O 170.482 g/mol
0.005 G_PER_L CHEBI:86318
copper(II) chloride dihydrate
19 Na2MoO4 x 2 H2O
2H2O.MoO4.2Na 241.946 g/mol
0.003 G_PER_L CHEBI:75213
sodium molybdate dihydrate
20 VOSO4 x 2 H2O
2H2O.O4S.OV 199.036 g/mol
0.003 G_PER_L CHEBI:87009
vanadyl sulfate dihydrate
21 CoSO4 x 7 H2O
Co.7H2O.O4S 281.101 g/mol
0.001 G_PER_L CHEBI:91244
cobalt(2+) sulfate heptahydrate
22 Biotin
C10H16N2O3S 244.316 g/mol
0.02 G_PER_L CHEBI:15956
biotin
23 Folic acid
C19H19N7O6 441.404 g/mol
0.02 G_PER_L CHEBI:27470
folic acid
24 Pyridoxine hydrochloride
C8H11NO3.HCl 205.641 g/mol
0.1 G_PER_L CHEBI:30961
pyridoxine hydrochloride
25 Thiamine HCl
C12H17N4OS.Cl.HCl 337.276 g/mol
0.05 G_PER_L CHEBI:49105
thiamine hydrochloride
26 Riboflavin
C17H20N4O6 376.369 g/mol
0.05 G_PER_L CHEBI:17015
riboflavin
27 Nicotinic acid
C6H5NO2 123.111 g/mol
0.05 G_PER_L CHEBI:15940
nicotinic acid
28 Calcium D-(+)-pantothenate
2C9H16NO5.Ca 476.536 g/mol
0.05 G_PER_L CHEBI:31345
Calcium pantothenate
29 Vitamin B12 0.001 G_PER_L CHEBI:176843
vitamin B12
30 p-Aminobenzoic acid
C7H7NO2 137.138 g/mol
0.05 G_PER_L CHEBI:30753
4-aminobenzoic acid
31 (DL)-alpha-Lipoic acid
C8H14O2S2 206.332 g/mol
0.05 G_PER_L CHEBI:16494
lipoic acid

Preparation

  1. Dissolve ingredients (except sulfur, vitamins, yeast extract and sulfide), adjust pH to 3.5 with 10 N H2SO4 and sparge medium with 80% N2 and 20% CO2 gas mixture for 30 - 45 min to make it anoxic. Dispense medium under same gas atmosphere into serum vials (e.g., 20 ml medium in 100 ml bottles) that contain already the appropriate amount of sulfur. Sterilize medium by heating cultivation vessels in a water bath to 90 - 100°C for 1 - 2 hours on each of 3 successive days. Add vitamins (sterilized by filtration) and yeast extract from sterile anoxic stock solutions prepared under 100% N2 gas. Prior to inoculation, add sulfide from a sterile anoxic stock solution prepared under 100% N2 gas and adjust pH of complete medium to 4.5, if necessary.
  2. Adjust pH of final solution to 2 with 1 N HCl.

Target organisms

No target organisms are recorded.

Provenance

TimestampCuratorActionNotes
2026-01-26T17:53:14.195576Z mediadive-import Imported from MediaDive Source: DSMZ, ID: 1360
2026-01-28T19:48:25.129846Z chebi-enrichment Added CHEBI ontology terms to ingredients Enriched using MicrobeMediaParam and MediaDive chemical mappings
2026-02-06T05:10:33.865341Z invalid-chebi-removal Removed 1 invalid CHEBI ID(s) Removed CHEBI IDs with invalid format (7+ digits) from MicrobeMediaParam source data
2026-03-13T06:18:58.643518Z culturemech-id-assigner-v1.0 Assigned CultureMech ID Assigned stable identifier: CultureMech:000821
2026-03-17T06:30:18.000000Z mediaingredientmech-enrichment-v1.0 Added MediaIngredientMech links Linked ingredients to MediaIngredientMech identifiers
2026-03-17T19:45:10.081122+00:00 normalize_media_names_to_snake_case.py NAME_NORMALIZED Normalized name from "HIPPEA JASONIAE MEDIUM" to "hippea_jasoniae_medium" for consistent matching
2026-06-05T07:41:08.530564+00:00 mim-legacy-to-chebi-migration-v1.0 Refreshed legacy MediaIngredientMech links to CHEBI keying Replaced 27 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme.
2026-06-10T07:46:59.768715+00:00 primary-term-label-canonicalization-v1.0 Canonicalized primary term label to OBO canonical (OAK) 3 primary term(s); ingredient name resolved by OAK sqlite:obo:chebi.