planctomycetales_bacterium_medium

CultureMech:000822 bacterial COMPLEX LIQUID
Original name: PLANCTOMYCETALES BACTERIUM MEDIUM

Overview

External ontology
mediadive.medium:1361 (source link unavailable) — PLANCTOMYCETALES BACTERIUM MEDIUM
Applications
  • Microbial cultivation
pH
7.0
Notes
Source: DSMZ | Link: https://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium1361.pdf

Composition

flowchart LR
    CultureMech_000822["planctomycetales_bacterium_medium"]
    ing0_KH2PO4["KH2PO4"]
    CultureMech_000822 --> ing0_KH2PO4
    CHEBI_63036["CHEBI:63036"]
    ing0_KH2PO4 -.-> CHEBI_63036
    ing1_MgCl2_x_6_H2O["MgCl2 x 6 H2O"]
    CultureMech_000822 --> ing1_MgCl2_x_6_H2O
    CHEBI_86345["CHEBI:86345"]
    ing1_MgCl2_x_6_H2O -.-> CHEBI_86345
    ing2_CaCl2_x_2_H2O["CaCl2 x 2 H2O"]
    CultureMech_000822 --> ing2_CaCl2_x_2_H2O
    CHEBI_86158["CHEBI:86158"]
    ing2_CaCl2_x_2_H2O -.-> CHEBI_86158
    ing3_NH4Cl["NH4Cl"]
    CultureMech_000822 --> ing3_NH4Cl
    CHEBI_31206["CHEBI:31206"]
    ing3_NH4Cl -.-> CHEBI_31206
    ing4_Glucose["Glucose"]
    CultureMech_000822 --> ing4_Glucose
    CHEBI_17234["CHEBI:17234"]
    ing4_Glucose -.-> CHEBI_17234
    ing5_Yeast_extract["Yeast extract"]
    CultureMech_000822 --> ing5_Yeast_extract
    ing6_Resazurin["Resazurin"]
    CultureMech_000822 --> ing6_Resazurin
    CHEBI_8806["CHEBI:8806"]
    ing6_Resazurin -.-> CHEBI_8806
    ing7_NaHCO3["NaHCO3"]
    CultureMech_000822 --> ing7_NaHCO3
    CHEBI_32139["CHEBI:32139"]
    ing7_NaHCO3 -.-> CHEBI_32139
    ing8_Na2S_x_9_H2O["Na2S x 9 H2O"]
    CultureMech_000822 --> ing8_Na2S_x_9_H2O
    CHEBI_76209["CHEBI:76209"]
    ing8_Na2S_x_9_H2O -.-> CHEBI_76209
    ing9_Cysteine_HCl["Cysteine HCl"]
    CultureMech_000822 --> ing9_Cysteine_HCl
    ing10_Biotin["Biotin"]
    CultureMech_000822 --> ing10_Biotin
    CHEBI_15956["CHEBI:15956"]
    ing10_Biotin -.-> CHEBI_15956
    ing11_Folic_acid["Folic acid"]
    CultureMech_000822 --> ing11_Folic_acid
    CHEBI_27470["CHEBI:27470"]
    ing11_Folic_acid -.-> CHEBI_27470
    ing12_Pyridoxine_hydrochloride["Pyridoxine hydrochloride"]
    CultureMech_000822 --> ing12_Pyridoxine_hydrochloride
    CHEBI_30961["CHEBI:30961"]
    ing12_Pyridoxine_hydrochloride -.-> CHEBI_30961
    ing13_Thiamine_HCl_x_2_H2O["Thiamine-HCl x 2 H2O"]
    CultureMech_000822 --> ing13_Thiamine_HCl_x_2_H2O
    CHEBI_132751["CHEBI:132751"]
    ing13_Thiamine_HCl_x_2_H2O -.-> CHEBI_132751
    ing14_Riboflavin["Riboflavin"]
    CultureMech_000822 --> ing14_Riboflavin
    CHEBI_17015["CHEBI:17015"]
    ing14_Riboflavin -.-> CHEBI_17015
    ing15_Nicotinic_acid["Nicotinic acid"]
    CultureMech_000822 --> ing15_Nicotinic_acid
    CHEBI_15940["CHEBI:15940"]
    ing15_Nicotinic_acid -.-> CHEBI_15940
    ing16_D_Calcium_pantothenate["D-Calcium pantothenate"]
    CultureMech_000822 --> ing16_D_Calcium_pantothenate
    CHEBI_31345["CHEBI:31345"]
    ing16_D_Calcium_pantothenate -.-> CHEBI_31345
    ing17_Vitamin_B12["Vitamin B12"]
    CultureMech_000822 --> ing17_Vitamin_B12
    CHEBI_176843["CHEBI:176843"]
    ing17_Vitamin_B12 -.-> CHEBI_176843
    ing18_p_Aminobenzoic_acid["p-Aminobenzoic acid"]
    CultureMech_000822 --> ing18_p_Aminobenzoic_acid
    CHEBI_30753["CHEBI:30753"]
    ing18_p_Aminobenzoic_acid -.-> CHEBI_30753
    ing19_Lipoic_acid["Lipoic acid"]
    CultureMech_000822 --> ing19_Lipoic_acid
    CHEBI_16494["CHEBI:16494"]
    ing19_Lipoic_acid -.-> CHEBI_16494
    ing20_Nitrilotriacetic_acid["Nitrilotriacetic acid"]
    CultureMech_000822 --> ing20_Nitrilotriacetic_acid
    CHEBI_44557["CHEBI:44557"]
    ing20_Nitrilotriacetic_acid -.-> CHEBI_44557
    ing21_FeCl3_x_6_H2O["FeCl3 x 6 H2O"]
    CultureMech_000822 --> ing21_FeCl3_x_6_H2O
    CHEBI_86254["CHEBI:86254"]
    ing21_FeCl3_x_6_H2O -.-> CHEBI_86254
    ing22_MnCl2_x_4_H2O["MnCl2 x 4 H2O"]
    CultureMech_000822 --> ing22_MnCl2_x_4_H2O
    CHEBI_86368["CHEBI:86368"]
    ing22_MnCl2_x_4_H2O -.-> CHEBI_86368
    ing23_CoCl2_x_6_H2O["CoCl2 x 6 H2O"]
    CultureMech_000822 --> ing23_CoCl2_x_6_H2O
    CHEBI_53503["CHEBI:53503"]
    ing23_CoCl2_x_6_H2O -.-> CHEBI_53503
    ing24_ZnCl2["ZnCl2"]
    CultureMech_000822 --> ing24_ZnCl2
    CHEBI_49976["CHEBI:49976"]
    ing24_ZnCl2 -.-> CHEBI_49976
    ing25_CuCl2_x_2_H2O["CuCl2 x 2 H2O"]
    CultureMech_000822 --> ing25_CuCl2_x_2_H2O
    CHEBI_86318["CHEBI:86318"]
    ing25_CuCl2_x_2_H2O -.-> CHEBI_86318
    ing26_H3BO3["H3BO3"]
    CultureMech_000822 --> ing26_H3BO3
    CHEBI_33118["CHEBI:33118"]
    ing26_H3BO3 -.-> CHEBI_33118
    ing27_Na2MoO4_x_2_H2O["Na2MoO4 x 2 H2O"]
    CultureMech_000822 --> ing27_Na2MoO4_x_2_H2O
    CHEBI_75213["CHEBI:75213"]
    ing27_Na2MoO4_x_2_H2O -.-> CHEBI_75213
    ing28_NiCl2_x_6_H2O["NiCl2 x 6 H2O"]
    CultureMech_000822 --> ing28_NiCl2_x_6_H2O
    CHEBI_34887["CHEBI:34887"]
    ing28_NiCl2_x_6_H2O -.-> CHEBI_34887
    ing29_Na2SeO3_x_5_H2O["Na2SeO3 x 5 H2O"]
    CultureMech_000822 --> ing29_Na2SeO3_x_5_H2O
    CHEBI_131361["CHEBI:131361"]
    ing29_Na2SeO3_x_5_H2O -.-> CHEBI_131361

Ingredients (30)

# Ingredient Concentration Ontology Notes
1 KH2PO4
H2O4P.K 136.084 g/mol
0.14 G_PER_L CHEBI:63036
potassium dihydrogen phosphate
2 MgCl2 x 6 H2O
2Cl.6H2O.Mg 203.301 g/mol
0.2 G_PER_L CHEBI:86345
magnesium dichloride hexahydrate
3 CaCl2 x 2 H2O
Ca.2Cl.2H2O 147.014 g/mol
0.25 G_PER_L CHEBI:86158
calcium chloride dihydrate
 [Merged 2 duplicates: 0.15, 0.1]
4 NH4Cl
Cl.H4N 53.492 g/mol
0.54 G_PER_L CHEBI:31206
ammonium chloride
5 Glucose
C6H12O6 180.156 g/mol
0.18 G_PER_L CHEBI:17234
glucose
6 Yeast extract 0.2 G_PER_L
7 Resazurin
C12H7NO4 229.191 g/mol
0.5 G_PER_L CHEBI:8806
Resazurin
8 NaHCO3
CHO3.Na 84.006 g/mol
2.5 G_PER_L CHEBI:32139
sodium hydrogencarbonate
9 Na2S x 9 H2O
9H2O.2Na.S 240.182 g/mol
0.3 G_PER_L CHEBI:76209
sodium sulfide nonahydrate
10 Cysteine HCl 0.3 G_PER_L
11 Biotin
C10H16N2O3S 244.316 g/mol
0.002 G_PER_L CHEBI:15956
biotin
12 Folic acid
C19H19N7O6 441.404 g/mol
0.002 G_PER_L CHEBI:27470
folic acid
13 Pyridoxine hydrochloride
C8H11NO3.HCl 205.641 g/mol
0.01 G_PER_L CHEBI:30961
pyridoxine hydrochloride
14 Thiamine-HCl x 2 H2O
C12H18N4OS.2Cl.2H2O 373.306 g/mol
0.005 G_PER_L CHEBI:132751
thiamine hydrochloride dihydrate
15 Riboflavin
C17H20N4O6 376.369 g/mol
0.005 G_PER_L CHEBI:17015
riboflavin
16 Nicotinic acid
C6H5NO2 123.111 g/mol
0.005 G_PER_L CHEBI:15940
nicotinic acid
17 D-Calcium pantothenate
2C9H16NO5.Ca 476.536 g/mol
0.005 G_PER_L CHEBI:31345
Calcium pantothenate
18 Vitamin B12 0.0001 G_PER_L CHEBI:176843
vitamin B12
19 p-Aminobenzoic acid
C7H7NO2 137.138 g/mol
0.005 G_PER_L CHEBI:30753
4-aminobenzoic acid
20 Lipoic acid
C8H14O2S2 206.332 g/mol
0.005 G_PER_L CHEBI:16494
lipoic acid
21 Nitrilotriacetic acid
C6H9NO6 191.139 g/mol
12.8 G_PER_L CHEBI:44557
nitrilotriacetic acid
22 FeCl3 x 6 H2O
Cl3Fe.6H2O 270.294 g/mol
1.35 G_PER_L CHEBI:86254
iron trichloride hexahydrate
23 MnCl2 x 4 H2O
Cl2Mn.4H2O 197.904 g/mol
0.1 G_PER_L CHEBI:86368
manganese(II) chloride tetrahydrate
24 CoCl2 x 6 H2O
2Cl.Co.6H2O 237.929 g/mol
0.024 G_PER_L CHEBI:53503
cobalt chloride hexahydrate
25 ZnCl2
2Cl.Zn 136.296 g/mol
0.1 G_PER_L CHEBI:49976
zinc dichloride
26 CuCl2 x 2 H2O
Cl2Cu.2H2O 170.482 g/mol
0.025 G_PER_L CHEBI:86318
copper(II) chloride dihydrate
27 H3BO3
H3BO3 61.833 g/mol
0.01 G_PER_L CHEBI:33118
boric acid
28 Na2MoO4 x 2 H2O
2H2O.MoO4.2Na 241.946 g/mol
0.024 G_PER_L CHEBI:75213
sodium molybdate dihydrate
29 NiCl2 x 6 H2O
Cl2Ni 129.599 g/mol
0.12 G_PER_L CHEBI:34887
nickel dichloride
30 Na2SeO3 x 5 H2O
5H2O.2Na.O3Se 263.012 g/mol
0.004 G_PER_L CHEBI:131361
disodium selenite pentahydrate

Preparation

  1. Prepare the medium without yeast extract, glucose, NaHCO3, cysteine hydrochloride, vitamin solution and Na2S x 9 H2O. Boil and then cool under a gas atmosphere of N2:CO2 (80:20 v/v). Dispense the medium into Hungate tubes or serum bottles under gassing with N2:CO2 (80:20 v/v) and add the NaHCO3. The final pH should be 7.0. Autoclave the medium and add from sterile stock solutions yeast extract, glucose, cysteine hydrochloride, vitamin solution and Na2S x 9 H2O.The final pH should be 7.0.

Target organisms

No target organisms are recorded.

Provenance

TimestampCuratorActionNotes
2026-01-26T17:53:14.200661Z mediadive-import Imported from MediaDive Source: DSMZ, ID: 1361
2026-01-28T19:48:35.310390Z chebi-enrichment Added CHEBI ontology terms to ingredients Enriched using MicrobeMediaParam and MediaDive chemical mappings
2026-03-13T03:09:30.437424Z data-quality-cleanup-v1.0 Data quality fixes Merged 1 duplicate ingredient(s)
2026-03-13T06:18:58.657645Z culturemech-id-assigner-v1.0 Assigned CultureMech ID Assigned stable identifier: CultureMech:000822
2026-03-17T06:30:40.000000Z mediaingredientmech-enrichment-v1.0 Added MediaIngredientMech links Linked ingredients to MediaIngredientMech identifiers
2026-03-17T19:45:10.096594+00:00 normalize_media_names_to_snake_case.py NAME_NORMALIZED Normalized name from "PLANCTOMYCETALES BACTERIUM MEDIUM" to "planctomycetales_bacterium_medium" for consistent matching
2026-06-05T07:41:42.180345+00:00 mim-legacy-to-chebi-migration-v1.0 Refreshed legacy MediaIngredientMech links to CHEBI keying Replaced 28 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme.
2026-06-10T07:47:10.295184+00:00 primary-term-label-canonicalization-v1.0 Canonicalized primary term label to OBO canonical (OAK) 3 primary term(s); ingredient name resolved by OAK sqlite:obo:chebi.