ectothiorhodospira_magna_medium

CultureMech:000829 bacterial COMPLEX LIQUID
Original name: ECTOTHIORHODOSPIRA MAGNA MEDIUM

Overview

External ontology
mediadive.medium:1369 (source link unavailable) — ECTOTHIORHODOSPIRA MAGNA MEDIUM
Applications
  • Microbial cultivation
pH range
{'min': 9.0, 'max': 9.5}
Notes
Source: DSMZ | Link: https://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium1369.pdf

Composition

flowchart LR
    CultureMech_000829["ectothiorhodospira_magna_medium"]
    ing0_KH2PO4["KH2PO4"]
    CultureMech_000829 --> ing0_KH2PO4
    CHEBI_63036["CHEBI:63036"]
    ing0_KH2PO4 -.-> CHEBI_63036
    ing1_NaCl["NaCl"]
    CultureMech_000829 --> ing1_NaCl
    CHEBI_26710["CHEBI:26710"]
    ing1_NaCl -.-> CHEBI_26710
    ing2_NH4Cl["NH4Cl"]
    CultureMech_000829 --> ing2_NH4Cl
    CHEBI_31206["CHEBI:31206"]
    ing2_NH4Cl -.-> CHEBI_31206
    ing3_MgCl2_x_6_H2O["MgCl2 x 6 H2O"]
    CultureMech_000829 --> ing3_MgCl2_x_6_H2O
    CHEBI_86345["CHEBI:86345"]
    ing3_MgCl2_x_6_H2O -.-> CHEBI_86345
    ing4_Na_acetate["Na-acetate"]
    CultureMech_000829 --> ing4_Na_acetate
    CHEBI_32954["CHEBI:32954"]
    ing4_Na_acetate -.-> CHEBI_32954
    ing5_Na2S2O3["Na2S2O3"]
    CultureMech_000829 --> ing5_Na2S2O3
    CHEBI_132112["CHEBI:132112"]
    ing5_Na2S2O3 -.-> CHEBI_132112
    ing6_Na2S_x_9_H2O["Na2S x 9 H2O"]
    CultureMech_000829 --> ing6_Na2S_x_9_H2O
    CHEBI_76209["CHEBI:76209"]
    ing6_Na2S_x_9_H2O -.-> CHEBI_76209
    ing7_NaHCO3["NaHCO3"]
    CultureMech_000829 --> ing7_NaHCO3
    CHEBI_32139["CHEBI:32139"]
    ing7_NaHCO3 -.-> CHEBI_32139
    ing8_Na2CO3["Na2CO3"]
    CultureMech_000829 --> ing8_Na2CO3
    CHEBI_29377["CHEBI:29377"]
    ing8_Na2CO3 -.-> CHEBI_29377
    ing9_Yeast_extract["Yeast extract"]
    CultureMech_000829 --> ing9_Yeast_extract
    ing10_Vitamin_B12["Vitamin B12"]
    CultureMech_000829 --> ing10_Vitamin_B12
    CHEBI_176843["CHEBI:176843"]
    ing10_Vitamin_B12 -.-> CHEBI_176843
    ing11_EDTA["EDTA"]
    CultureMech_000829 --> ing11_EDTA
    CHEBI_4735["CHEBI:4735"]
    ing11_EDTA -.-> CHEBI_4735
    ing12_FeSO4_x_7_H2O["FeSO4 x 7 H2O"]
    CultureMech_000829 --> ing12_FeSO4_x_7_H2O
    CHEBI_75836["CHEBI:75836"]
    ing12_FeSO4_x_7_H2O -.-> CHEBI_75836
    ing13_ZnSO4_x_7_H2O["ZnSO4 x 7 H2O"]
    CultureMech_000829 --> ing13_ZnSO4_x_7_H2O
    CHEBI_32312["CHEBI:32312"]
    ing13_ZnSO4_x_7_H2O -.-> CHEBI_32312
    ing14_MnCl2_x_4_H2O["MnCl2 x 4 H2O"]
    CultureMech_000829 --> ing14_MnCl2_x_4_H2O
    CHEBI_86368["CHEBI:86368"]
    ing14_MnCl2_x_4_H2O -.-> CHEBI_86368
    ing15_H3BO3["H3BO3"]
    CultureMech_000829 --> ing15_H3BO3
    CHEBI_33118["CHEBI:33118"]
    ing15_H3BO3 -.-> CHEBI_33118
    ing16_CoCl2_x_6_H2O["CoCl2 x 6 H2O"]
    CultureMech_000829 --> ing16_CoCl2_x_6_H2O
    CHEBI_53503["CHEBI:53503"]
    ing16_CoCl2_x_6_H2O -.-> CHEBI_53503
    ing17_CuCl2_x_2_H2O["CuCl2 x 2 H2O"]
    CultureMech_000829 --> ing17_CuCl2_x_2_H2O
    CHEBI_86318["CHEBI:86318"]
    ing17_CuCl2_x_2_H2O -.-> CHEBI_86318
    ing18_NiCl2_x_6_H2O["NiCl2 x 6 H2O"]
    CultureMech_000829 --> ing18_NiCl2_x_6_H2O
    CHEBI_34887["CHEBI:34887"]
    ing18_NiCl2_x_6_H2O -.-> CHEBI_34887
    ing19_Na2MoO4_x_2_H2O["Na2MoO4 x 2 H2O"]
    CultureMech_000829 --> ing19_Na2MoO4_x_2_H2O
    CHEBI_75213["CHEBI:75213"]
    ing19_Na2MoO4_x_2_H2O -.-> CHEBI_75213

Ingredients (20)

# Ingredient Concentration Ontology Notes
1 KH2PO4
H2O4P.K 136.084 g/mol
0.5 G_PER_L CHEBI:63036
potassium dihydrogen phosphate
2 NaCl
Cl.Na 58.443 g/mol
30 G_PER_L CHEBI:26710
sodium chloride
3 NH4Cl
Cl.H4N 53.492 g/mol
0.5 G_PER_L CHEBI:31206
ammonium chloride
4 MgCl2 x 6 H2O
2Cl.6H2O.Mg 203.301 g/mol
0.2 G_PER_L CHEBI:86345
magnesium dichloride hexahydrate
5 Na-acetate
C2H3O2.Na 82.034 g/mol
1 G_PER_L CHEBI:32954
sodium acetate
6 Na2S2O3
2Na.O3S2 158.111 g/mol
0.5 G_PER_L CHEBI:132112
sodium thiosulfate
7 Na2S x 9 H2O
9H2O.2Na.S 240.182 g/mol
0.5 G_PER_L CHEBI:76209
sodium sulfide nonahydrate
8 NaHCO3
CHO3.Na 84.006 g/mol
5 G_PER_L CHEBI:32139
sodium hydrogencarbonate
9 Na2CO3
CO3.2Na 105.988 g/mol
5 G_PER_L CHEBI:29377
sodium carbonate
10 Yeast extract 0.1 G_PER_L
11 Vitamin B12 1e-05 G_PER_L CHEBI:176843
vitamin B12
12 EDTA
C10H16N2O8 292.244 g/mol
5 G_PER_L CHEBI:4735
ethylenediaminetetraacetic acid
13 FeSO4 x 7 H2O
Fe.7H2O.O4S 278.013 g/mol
2.2 G_PER_L CHEBI:75836
iron(2+) sulfate heptahydrate
14 ZnSO4 x 7 H2O
7H2O.O4S.Zn 287.558 g/mol
0.1 G_PER_L CHEBI:32312
zinc sulfate heptahydrate
15 MnCl2 x 4 H2O
Cl2Mn.4H2O 197.904 g/mol
0.03 G_PER_L CHEBI:86368
manganese(II) chloride tetrahydrate
16 H3BO3
H3BO3 61.833 g/mol
0.03 G_PER_L CHEBI:33118
boric acid
17 CoCl2 x 6 H2O
2Cl.Co.6H2O 237.929 g/mol
0.2 G_PER_L CHEBI:53503
cobalt chloride hexahydrate
18 CuCl2 x 2 H2O
Cl2Cu.2H2O 170.482 g/mol
0.03 G_PER_L CHEBI:86318
copper(II) chloride dihydrate
19 NiCl2 x 6 H2O
Cl2Ni 129.599 g/mol
0.03 G_PER_L CHEBI:34887
nickel dichloride
20 Na2MoO4 x 2 H2O
2H2O.MoO4.2Na 241.946 g/mol
0.03 G_PER_L CHEBI:75213
sodium molybdate dihydrate

Preparation

  1. pH 9.0-9.5
  2. Prepare the medium without the NaHCO3, Na2S x 9 H2O, Na2CO3 and vitamin B12, under a nitrogen atmosphere.
  3. Autoclave and add the vitamin B12 from a filter-sterilised stock solution and the NaHCO3 (autoclaved in sealed, half full vessels), Na2S x 9 H2O, Na2CO3 from sterile stock solutions. The final pH of the medium should be 9.0-9.5.
  4. pH 3.0-4.0

Target organisms

No target organisms are recorded.

Provenance

TimestampCuratorActionNotes
2026-01-26T17:53:14.221510Z mediadive-import Imported from MediaDive Source: DSMZ, ID: 1369
2026-03-13T06:18:58.700633Z culturemech-id-assigner-v1.0 Assigned CultureMech ID Assigned stable identifier: CultureMech:000829
2026-03-17T06:30:03.000000Z mediaingredientmech-enrichment-v1.0 Added MediaIngredientMech links Linked ingredients to MediaIngredientMech identifiers
2026-03-17T19:45:10.143997+00:00 normalize_media_names_to_snake_case.py NAME_NORMALIZED Normalized name from "ECTOTHIORHODOSPIRA MAGNA MEDIUM" to "ectothiorhodospira_magna_medium" for consistent matching
2026-05-20T06:01:16.048678+00:00 migrate_ph_range.py MIGRATED_PH_RANGE ph_range '9-9.5' -> {'min': 9.0, 'max': 9.5}
2026-06-05T07:40:59.322975+00:00 mim-legacy-to-chebi-migration-v1.0 Refreshed legacy MediaIngredientMech links to CHEBI keying Replaced 18 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme.
2026-06-10T07:37:34.787749+00:00 primary-term-regrounding-v1.0 Re-grounded wrong primary term id (OAK-verified) 1 primary term(s); ingredient name resolved by OAK sqlite:obo:chebi.
2026-06-10T07:46:56.621963+00:00 primary-term-label-canonicalization-v1.0 Canonicalized primary term label to OBO canonical (OAK) 6 primary term(s); ingredient name resolved by OAK sqlite:obo:chebi.