Overview
- External ontology
- mediadive.medium:1371 (source link unavailable) — NBRC 274 MEDIUM
- Applications
-
- Microbial cultivation
- pH
- 7.2
- Notes
- Source: DSMZ | Link: https://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium1371.pdf
Composition
flowchart LR
CultureMech_000832["nbrc_274_medium"]
ing0_Bacto_Tryptone["Bacto Tryptone"]
CultureMech_000832 --> ing0_Bacto_Tryptone
ing1_Beef_extract["Beef extract"]
CultureMech_000832 --> ing1_Beef_extract
ing2_Yeast_extract["Yeast extract"]
CultureMech_000832 --> ing2_Yeast_extract
ing3_Sodium_acetate["Sodium acetate"]
CultureMech_000832 --> ing3_Sodium_acetate
CHEBI_32954["CHEBI:32954"]
ing3_Sodium_acetate -.-> CHEBI_32954
ing4_Agar["Agar"]
CultureMech_000832 --> ing4_Agar
CHEBI_2509["CHEBI:2509"]
ing4_Agar -.-> CHEBI_2509
Ingredients (5)
| # | Ingredient | Concentration | Ontology | Notes |
|---|---|---|---|---|
| 1 | Bacto Tryptone | 0.5 G_PER_L |
MICRO:0000182 (source link unavailable)
Tryptone
|
|
| 2 | Beef extract | 0.2 G_PER_L |
FOODON:03302088
Beef extract
|
|
| 3 | Yeast extract | 0.5 G_PER_L |
FOODON:03315426
Yeast extract
|
|
| 4 |
Sodium acetate
C2H3O2.Na 82.034 g/mol
|
0.2 G_PER_L |
CHEBI:32954
sodium acetate
|
|
| 5 | Agar | 15 G_PER_L |
CHEBI:2509
agar
|
(for solid medium) |
Preparation
- Adjust pH to 7.2. The medium may be solidified by adding 15 g/l agar.
Target organisms
No target organisms are recorded.
Provenance
| Timestamp | Curator | Action | Notes |
|---|---|---|---|
| 2026-01-26T17:53:14.227901Z | mediadive-import | Imported from MediaDive | Source: DSMZ, ID: 1371 |
| 2026-03-13T06:18:58.719997Z | culturemech-id-assigner-v1.0 | Assigned CultureMech ID | Assigned stable identifier: CultureMech:000832 |
| 2026-03-17T06:30:11.000000Z | mediaingredientmech-enrichment-v1.0 | Added MediaIngredientMech links | Linked ingredients to MediaIngredientMech identifiers |
| 2026-03-17T19:45:10.166425+00:00 | normalize_media_names_to_snake_case.py | NAME_NORMALIZED | Normalized name from "NBRC 274 MEDIUM" to "nbrc_274_medium" for consistent matching |
| 2026-06-05T07:41:39.516446+00:00 | mim-legacy-to-chebi-migration-v1.0 | Refreshed legacy MediaIngredientMech links to CHEBI keying | Replaced 2 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme. |
| 2026-09-13T00:00:00-07:00 | repair_bacterial_score10_exact_terms_batch6.py | GROUNDED_BACTERIAL_SCORE10_EXACT_TERMS_BATCH6 | Applied local mappings for Bacto Tryptone, Beef extract, Yeast extract. |
| 2026-10-07T13:32:40.086487+00:00 | codex | CURATED_MEDIUM_CONCENTRATION_SOURCE_EVIDENCE | Exact medium/source membership, ingredient inventory/forms, physical-state conditions, literal snippets, and arithmetic checked. Stock boundaries, source-only rows, preparation context, and variants remain explicit follow-up tasks; secondary chemical mappings unchanged. |