succinate_minimal_salts_medium_sms

CultureMech:000833 bacterial COMPLEX LIQUID
Original name: SUCCINATE MINIMAL SALTS MEDIUM (SMS)

Overview

External ontology
mediadive.medium:1372 (source link unavailable) — SUCCINATE MINIMAL SALTS MEDIUM (SMS)
Applications
  • Microbial cultivation
pH
6.8
Notes
Source: DSMZ | Link: https://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium1372.pdf

Composition

flowchart LR
    CultureMech_000833["succinate_minimal_salts_medium_sms"]
    ing0_EDTA["EDTA"]
    CultureMech_000833 --> ing0_EDTA
    CHEBI_4735["CHEBI:4735"]
    ing0_EDTA -.-> CHEBI_4735
    ing1_KH2PO4["KH2PO4"]
    CultureMech_000833 --> ing1_KH2PO4
    CHEBI_63036["CHEBI:63036"]
    ing1_KH2PO4 -.-> CHEBI_63036
    ing2_K2HPO4["K2HPO4"]
    CultureMech_000833 --> ing2_K2HPO4
    CHEBI_131527["CHEBI:131527"]
    ing2_K2HPO4 -.-> CHEBI_131527
    ing3_NH4Cl["NH4Cl"]
    CultureMech_000833 --> ing3_NH4Cl
    CHEBI_31206["CHEBI:31206"]
    ing3_NH4Cl -.-> CHEBI_31206
    ing4_MgSO4_x_7_H2O["MgSO4 x 7 H2O"]
    CultureMech_000833 --> ing4_MgSO4_x_7_H2O
    CHEBI_31795["CHEBI:31795"]
    ing4_MgSO4_x_7_H2O -.-> CHEBI_31795
    ing5_CaCl2_x_6_H2O["CaCl2 x 6 H2O"]
    CultureMech_000833 --> ing5_CaCl2_x_6_H2O
    CHEBI_91243["CHEBI:91243"]
    ing5_CaCl2_x_6_H2O -.-> CHEBI_91243
    ing6_Sodium_succinate["Sodium succinate"]
    CultureMech_000833 --> ing6_Sodium_succinate
    CHEBI_63675["CHEBI:63675"]
    ing6_Sodium_succinate -.-> CHEBI_63675
    ing7_Yeast_extract["Yeast extract"]
    CultureMech_000833 --> ing7_Yeast_extract
    ing8_FeSO4_x_6_H2O["FeSO4 x 6 H2O"]
    CultureMech_000833 --> ing8_FeSO4_x_6_H2O
    CHEBI_75832["CHEBI:75832"]
    ing8_FeSO4_x_6_H2O -.-> CHEBI_75832
    ing9_ZnSO4_x_7_H2O["ZnSO4 x 7 H2O"]
    CultureMech_000833 --> ing9_ZnSO4_x_7_H2O
    CHEBI_32312["CHEBI:32312"]
    ing9_ZnSO4_x_7_H2O -.-> CHEBI_32312
    ing10_MnCl2_x_4_H2O["MnCl2 x 4 H2O"]
    CultureMech_000833 --> ing10_MnCl2_x_4_H2O
    CHEBI_86368["CHEBI:86368"]
    ing10_MnCl2_x_4_H2O -.-> CHEBI_86368
    ing11_H3BO3["H3BO3"]
    CultureMech_000833 --> ing11_H3BO3
    CHEBI_33118["CHEBI:33118"]
    ing11_H3BO3 -.-> CHEBI_33118
    ing12_CoCl2_x_6_H2O["CoCl2 x 6 H2O"]
    CultureMech_000833 --> ing12_CoCl2_x_6_H2O
    CHEBI_53503["CHEBI:53503"]
    ing12_CoCl2_x_6_H2O -.-> CHEBI_53503
    ing13_CuCl2_x_2_H2O["CuCl2 x 2 H2O"]
    CultureMech_000833 --> ing13_CuCl2_x_2_H2O
    CHEBI_86318["CHEBI:86318"]
    ing13_CuCl2_x_2_H2O -.-> CHEBI_86318
    ing14_NiCl2_x_6_H2O["NiCl2 x 6 H2O"]
    CultureMech_000833 --> ing14_NiCl2_x_6_H2O
    CHEBI_34887["CHEBI:34887"]
    ing14_NiCl2_x_6_H2O -.-> CHEBI_34887
    ing15_Na2MoO4_x_2_H2O["Na2MoO4 x 2 H2O"]
    CultureMech_000833 --> ing15_Na2MoO4_x_2_H2O
    CHEBI_75213["CHEBI:75213"]
    ing15_Na2MoO4_x_2_H2O -.-> CHEBI_75213
    ing16_Biotin["Biotin"]
    CultureMech_000833 --> ing16_Biotin
    CHEBI_15956["CHEBI:15956"]
    ing16_Biotin -.-> CHEBI_15956
    ing17_Thiamine_HCl_x_2_H2O["Thiamine-HCl x 2 H2O"]
    CultureMech_000833 --> ing17_Thiamine_HCl_x_2_H2O
    CHEBI_132751["CHEBI:132751"]
    ing17_Thiamine_HCl_x_2_H2O -.-> CHEBI_132751
    ing18_Nicotinic_acid["Nicotinic acid"]
    CultureMech_000833 --> ing18_Nicotinic_acid
    CHEBI_15940["CHEBI:15940"]
    ing18_Nicotinic_acid -.-> CHEBI_15940
    ing19_Vitamin_B12["Vitamin B12"]
    CultureMech_000833 --> ing19_Vitamin_B12
    CHEBI_176843["CHEBI:176843"]
    ing19_Vitamin_B12 -.-> CHEBI_176843

Ingredients (20)

# Ingredient Concentration Ontology Notes
1 EDTA
C10H16N2O8 292.244 g/mol
0.01 G_PER_L CHEBI:4735
ethylenediaminetetraacetic acid
2 KH2PO4
H2O4P.K 136.084 g/mol
0.6 G_PER_L CHEBI:63036
potassium dihydrogen phosphate
3 K2HPO4
HO4P.2K 174.174 g/mol
0.9 G_PER_L CHEBI:131527
dipotassium hydrogen phosphate
4 NH4Cl
Cl.H4N 53.492 g/mol
1 G_PER_L CHEBI:31206
ammonium chloride
5 MgSO4 x 7 H2O
7H2O.Mg.O4S 246.473 g/mol
0.2 G_PER_L CHEBI:31795
magnesium sulfate heptahydrate
6 CaCl2 x 6 H2O
Ca.2Cl.6H2O 219.074 g/mol
0.075 G_PER_L CHEBI:91243
calcium chloride hexahydrate
7 Sodium succinate
C4H4O4.2Na 162.052 g/mol
2.2 G_PER_L CHEBI:63675
sodium succinate (anhydrous)
8 Yeast extract 0.1 G_PER_L
9 FeSO4 x 6 H2O
Fe.O4S 151.908 g/mol
0.3 G_PER_L CHEBI:75832
iron(2+) sulfate (anhydrous)
10 ZnSO4 x 7 H2O
7H2O.O4S.Zn 287.558 g/mol
0.005 G_PER_L CHEBI:32312
zinc sulfate heptahydrate
11 MnCl2 x 4 H2O
Cl2Mn.4H2O 197.904 g/mol
0.003 G_PER_L CHEBI:86368
manganese(II) chloride tetrahydrate
12 H3BO3
H3BO3 61.833 g/mol
0.002 G_PER_L CHEBI:33118
boric acid
13 CoCl2 x 6 H2O
2Cl.Co.6H2O 237.929 g/mol
0.005 G_PER_L CHEBI:53503
cobalt chloride hexahydrate
14 CuCl2 x 2 H2O
Cl2Cu.2H2O 170.482 g/mol
0.001 G_PER_L CHEBI:86318
copper(II) chloride dihydrate
15 NiCl2 x 6 H2O
Cl2Ni 129.599 g/mol
0.002 G_PER_L CHEBI:34887
nickel dichloride
16 Na2MoO4 x 2 H2O
2H2O.MoO4.2Na 241.946 g/mol
0.003 G_PER_L CHEBI:75213
sodium molybdate dihydrate
17 Biotin
C10H16N2O3S 244.316 g/mol
0.08 G_PER_L CHEBI:15956
biotin
18 Thiamine-HCl x 2 H2O
C12H18N4OS.2Cl.2H2O 373.306 g/mol
0.4 G_PER_L CHEBI:132751
thiamine hydrochloride dihydrate
19 Nicotinic acid
C6H5NO2 123.111 g/mol
0.4 G_PER_L CHEBI:15940
nicotinic acid
20 Vitamin B12 0.02 G_PER_L CHEBI:176843
vitamin B12

Preparation

  1. Adjust pH to 6.8.
  2. Prepare the medium without the vitamin solution.
  3. Autoclave and add the vitamin solution from a filter-sterilised stock solution.
  4. Incubate strains in liquid culture with air headspace in closed vessels.

Target organisms

No target organisms are recorded.

Provenance

TimestampCuratorActionNotes
2026-01-26T17:53:14.229353Z mediadive-import Imported from MediaDive Source: DSMZ, ID: 1372
2026-03-13T06:18:58.727615Z culturemech-id-assigner-v1.0 Assigned CultureMech ID Assigned stable identifier: CultureMech:000833
2026-03-17T06:30:57.000000Z mediaingredientmech-enrichment-v1.0 Added MediaIngredientMech links Linked ingredients to MediaIngredientMech identifiers
2026-03-17T19:45:10.174485+00:00 normalize_media_names_to_snake_case.py NAME_NORMALIZED Normalized name from "SUCCINATE MINIMAL SALTS MEDIUM (SMS)" to "succinate_minimal_salts_medium_sms" for consistent matching
2026-06-05T07:41:47.205154+00:00 mim-legacy-to-chebi-migration-v1.0 Refreshed legacy MediaIngredientMech links to CHEBI keying Replaced 19 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme.
2026-06-10T07:47:11.920015+00:00 primary-term-label-canonicalization-v1.0 Canonicalized primary term label to OBO canonical (OAK) 4 primary term(s); ingredient name resolved by OAK sqlite:obo:chebi.