mo_cfxi_medium

CultureMech:000839 bacterial COMPLEX LIQUID
Original name: MO-CFXI MEDIUM

Overview

External ontology
mediadive.medium:1378 (source link unavailable) — MO-CFXI MEDIUM
Applications
  • Microbial cultivation
pH
7.0
Notes
Source: DSMZ | Link: https://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium1378.pdf

Composition

flowchart LR
    CultureMech_000839["mo_cfxi_medium"]
    ing0_NaCl["NaCl"]
    CultureMech_000839 --> ing0_NaCl
    CHEBI_26710["CHEBI:26710"]
    ing0_NaCl -.-> CHEBI_26710
    ing1_MgCl2_x_6_H2O["MgCl2 x 6 H2O"]
    CultureMech_000839 --> ing1_MgCl2_x_6_H2O
    CHEBI_86345["CHEBI:86345"]
    ing1_MgCl2_x_6_H2O -.-> CHEBI_86345
    ing2_KCl["KCl"]
    CultureMech_000839 --> ing2_KCl
    CHEBI_32588["CHEBI:32588"]
    ing2_KCl -.-> CHEBI_32588
    ing3_CaCl2_x_2_H2O["CaCl2 x 2 H2O"]
    CultureMech_000839 --> ing3_CaCl2_x_2_H2O
    CHEBI_86158["CHEBI:86158"]
    ing3_CaCl2_x_2_H2O -.-> CHEBI_86158
    ing4_NH4Cl["NH4Cl"]
    CultureMech_000839 --> ing4_NH4Cl
    CHEBI_31206["CHEBI:31206"]
    ing4_NH4Cl -.-> CHEBI_31206
    ing5_KH2PO4["KH2PO4"]
    CultureMech_000839 --> ing5_KH2PO4
    CHEBI_63036["CHEBI:63036"]
    ing5_KH2PO4 -.-> CHEBI_63036
    ing6_Tryptone["Tryptone"]
    CultureMech_000839 --> ing6_Tryptone
    ing7_Glucose["Glucose"]
    CultureMech_000839 --> ing7_Glucose
    CHEBI_17234["CHEBI:17234"]
    ing7_Glucose -.-> CHEBI_17234
    ing8_Sodium_pyruvate["Sodium pyruvate"]
    CultureMech_000839 --> ing8_Sodium_pyruvate
    CHEBI_50144["CHEBI:50144"]
    ing8_Sodium_pyruvate -.-> CHEBI_50144
    ing9_Yeast_extract["Yeast extract"]
    CultureMech_000839 --> ing9_Yeast_extract
    ing10_NaHCO3["NaHCO3"]
    CultureMech_000839 --> ing10_NaHCO3
    CHEBI_32139["CHEBI:32139"]
    ing10_NaHCO3 -.-> CHEBI_32139
    ing11_Cysteine_HCl["Cysteine-HCl"]
    CultureMech_000839 --> ing11_Cysteine_HCl
    CHEBI_91247["CHEBI:91247"]
    ing11_Cysteine_HCl -.-> CHEBI_91247
    ing12_Resazurin["Resazurin"]
    CultureMech_000839 --> ing12_Resazurin
    CHEBI_8806["CHEBI:8806"]
    ing12_Resazurin -.-> CHEBI_8806
    ing13_Na2S["Na2S"]
    CultureMech_000839 --> ing13_Na2S
    CHEBI_76208["CHEBI:76208"]
    ing13_Na2S -.-> CHEBI_76208
    ing14_FeCl2_x_4_H2O["FeCl2 x 4 H2O"]
    CultureMech_000839 --> ing14_FeCl2_x_4_H2O
    CHEBI_86249["CHEBI:86249"]
    ing14_FeCl2_x_4_H2O -.-> CHEBI_86249
    ing15_ZnCl2["ZnCl2"]
    CultureMech_000839 --> ing15_ZnCl2
    CHEBI_49976["CHEBI:49976"]
    ing15_ZnCl2 -.-> CHEBI_49976
    ing16_MnCl2_x_4_H2O["MnCl2 x 4 H2O"]
    CultureMech_000839 --> ing16_MnCl2_x_4_H2O
    CHEBI_86368["CHEBI:86368"]
    ing16_MnCl2_x_4_H2O -.-> CHEBI_86368
    ing17_Na2SeO4["Na2SeO4"]
    CultureMech_000839 --> ing17_Na2SeO4
    CHEBI_77775["CHEBI:77775"]
    ing17_Na2SeO4 -.-> CHEBI_77775
    ing18_H3BO3["H3BO3"]
    CultureMech_000839 --> ing18_H3BO3
    CHEBI_33118["CHEBI:33118"]
    ing18_H3BO3 -.-> CHEBI_33118
    ing19_Na2WO4_x_2_H2O["Na2WO4 x 2 H2O"]
    CultureMech_000839 --> ing19_Na2WO4_x_2_H2O
    CHEBI_63939["CHEBI:63939"]
    ing19_Na2WO4_x_2_H2O -.-> CHEBI_63939
    ing20_CuCl2_x_2_H2O["CuCl2 x 2 H2O"]
    CultureMech_000839 --> ing20_CuCl2_x_2_H2O
    CHEBI_86318["CHEBI:86318"]
    ing20_CuCl2_x_2_H2O -.-> CHEBI_86318
    ing21_CoCl2_x_6_H2O["CoCl2 x 6 H2O"]
    CultureMech_000839 --> ing21_CoCl2_x_6_H2O
    CHEBI_53503["CHEBI:53503"]
    ing21_CoCl2_x_6_H2O -.-> CHEBI_53503
    ing22_AlCl3["AlCl3"]
    CultureMech_000839 --> ing22_AlCl3
    CHEBI_30114["CHEBI:30114"]
    ing22_AlCl3 -.-> CHEBI_30114
    ing23_NiCl2["NiCl2"]
    CultureMech_000839 --> ing23_NiCl2
    CHEBI_34887["CHEBI:34887"]
    ing23_NiCl2 -.-> CHEBI_34887
    ing24_Na2MoO4_x_2_H2O["Na2MoO4 x 2 H2O"]
    CultureMech_000839 --> ing24_Na2MoO4_x_2_H2O
    CHEBI_75213["CHEBI:75213"]
    ing24_Na2MoO4_x_2_H2O -.-> CHEBI_75213
    ing25_Biotin["Biotin"]
    CultureMech_000839 --> ing25_Biotin
    CHEBI_15956["CHEBI:15956"]
    ing25_Biotin -.-> CHEBI_15956
    ing26_Folic_acid["Folic acid"]
    CultureMech_000839 --> ing26_Folic_acid
    CHEBI_27470["CHEBI:27470"]
    ing26_Folic_acid -.-> CHEBI_27470
    ing27_Pyridoxine_hydrochloride["Pyridoxine hydrochloride"]
    CultureMech_000839 --> ing27_Pyridoxine_hydrochloride
    CHEBI_30961["CHEBI:30961"]
    ing27_Pyridoxine_hydrochloride -.-> CHEBI_30961
    ing28_Thiamine_HCl_x_2_H2O["Thiamine-HCl x 2 H2O"]
    CultureMech_000839 --> ing28_Thiamine_HCl_x_2_H2O
    CHEBI_132751["CHEBI:132751"]
    ing28_Thiamine_HCl_x_2_H2O -.-> CHEBI_132751
    ing29_Riboflavin["Riboflavin"]
    CultureMech_000839 --> ing29_Riboflavin
    CHEBI_17015["CHEBI:17015"]
    ing29_Riboflavin -.-> CHEBI_17015
    CultureMech_000839 --> more["...5 more"]

Ingredients (35)

# Ingredient Concentration Ontology Notes
1 NaCl
Cl.Na 58.443 g/mol
25 G_PER_L CHEBI:26710
sodium chloride
2 MgCl2 x 6 H2O
2Cl.6H2O.Mg 203.301 g/mol
4 G_PER_L CHEBI:86345
magnesium dichloride hexahydrate
3 KCl
Cl.K 74.551 g/mol
0.5 G_PER_L CHEBI:32588
potassium chloride
4 CaCl2 x 2 H2O
Ca.2Cl.2H2O 147.014 g/mol
1 G_PER_L CHEBI:86158
calcium chloride dihydrate
5 NH4Cl
Cl.H4N 53.492 g/mol
0.535 G_PER_L CHEBI:31206
ammonium chloride
6 KH2PO4
H2O4P.K 136.084 g/mol
0.1 G_PER_L CHEBI:63036
potassium dihydrogen phosphate
7 Tryptone 1 G_PER_L
8 Glucose
C6H12O6 180.156 g/mol
1.8 G_PER_L CHEBI:17234
glucose
9 Sodium pyruvate
C3H3O3.Na 110.044 g/mol
0.55 G_PER_L CHEBI:50144
sodium pyruvate
10 Yeast extract 1 G_PER_L
11 NaHCO3
CHO3.Na 84.006 g/mol
2 G_PER_L CHEBI:32139
sodium hydrogencarbonate
12 Cysteine-HCl
C3H7NO2S.HCl 157.622 g/mol
0.3 G_PER_L CHEBI:91247
L-cysteine hydrochloride
13 Resazurin
C12H7NO4 229.191 g/mol
1 G_PER_L CHEBI:8806
Resazurin
14 Na2S
2Na.S 78.047 g/mol
0.3 G_PER_L CHEBI:76208
sodium sulfide (anhydrous)
15 FeCl2 x 4 H2O
Cl2Fe.4H2O 198.811 g/mol
1.27 G_PER_L CHEBI:86249
iron dichloride tetrahydrate
16 ZnCl2
2Cl.Zn 136.296 g/mol
0.14 G_PER_L CHEBI:49976
zinc dichloride
17 MnCl2 x 4 H2O
Cl2Mn.4H2O 197.904 g/mol
0.2 G_PER_L CHEBI:86368
manganese(II) chloride tetrahydrate
18 Na2SeO4
2Na.O4Se 188.936 g/mol
0.002 G_PER_L CHEBI:77775
sodium selenate
19 H3BO3
H3BO3 61.833 g/mol
0.006 G_PER_L CHEBI:33118
boric acid
20 Na2WO4 x 2 H2O
2H2O.2Na.O4W 329.846 g/mol
0.003 G_PER_L CHEBI:63939
sodium tungstate dihydrate
21 CuCl2 x 2 H2O
Cl2Cu.2H2O 170.482 g/mol
0.001 G_PER_L CHEBI:86318
copper(II) chloride dihydrate
22 CoCl2 x 6 H2O
2Cl.Co.6H2O 237.929 g/mol
0.13 G_PER_L CHEBI:53503
cobalt chloride hexahydrate
23 AlCl3
AlCl3 133.341 g/mol
0.01 G_PER_L CHEBI:30114
aluminium trichloride
24 NiCl2
Cl2Ni 129.599 g/mol
0.01 G_PER_L CHEBI:34887
nickel dichloride
25 Na2MoO4 x 2 H2O
2H2O.MoO4.2Na 241.946 g/mol
0.02 G_PER_L CHEBI:75213
sodium molybdate dihydrate
26 Biotin
C10H16N2O3S 244.316 g/mol
0.0049 G_PER_L CHEBI:15956
biotin
27 Folic acid
C19H19N7O6 441.404 g/mol
0.0088 G_PER_L CHEBI:27470
folic acid
28 Pyridoxine hydrochloride
C8H11NO3.HCl 205.641 g/mol
0.0041 G_PER_L CHEBI:30961
pyridoxine hydrochloride
29 Thiamine-HCl x 2 H2O
C12H18N4OS.2Cl.2H2O 373.306 g/mol
0.0067 G_PER_L CHEBI:132751
thiamine hydrochloride dihydrate
30 Riboflavin
C17H20N4O6 376.369 g/mol
0.0075 G_PER_L CHEBI:17015
riboflavin
31 Nicotinic acid
C6H5NO2 123.111 g/mol
0.0024 G_PER_L CHEBI:15940
nicotinic acid
32 D-Calcium pantothenate
2C9H16NO5.Ca 476.536 g/mol
0.0095 G_PER_L CHEBI:31345
Calcium pantothenate
33 Vitamin B12 0.0001 G_PER_L CHEBI:176843
vitamin B12
34 p-Aminobenzoic acid
C7H7NO2 137.138 g/mol
0.0027 G_PER_L CHEBI:30753
4-aminobenzoic acid
35 Lipoic acid
C8H14O2S2 206.332 g/mol
0.0041 G_PER_L CHEBI:16494
lipoic acid

Preparation

  1. pH 7.0
  2. Prepare under N2/CO2 (80/20) gas.
  3. Autoclave and add the following components after autoclaving from stock solutions:
  4. sterile Na2S solution end concentration 0.03%
  5. and filter-sterile Vitamin solution (see below) 0.2 ml / 100 ml.

Target organisms

No target organisms are recorded.

Provenance

TimestampCuratorActionNotes
2026-01-26T17:53:14.250687Z mediadive-import Imported from MediaDive Source: DSMZ, ID: 1378
2026-01-28T19:48:26.718566Z chebi-enrichment Added CHEBI ontology terms to ingredients Enriched using MicrobeMediaParam and MediaDive chemical mappings
2026-03-13T06:18:58.796035Z culturemech-id-assigner-v1.0 Assigned CultureMech ID Assigned stable identifier: CultureMech:000839
2026-03-17T06:30:22.000000Z mediaingredientmech-enrichment-v1.0 Added MediaIngredientMech links Linked ingredients to MediaIngredientMech identifiers
2026-03-17T19:45:10.248870+00:00 normalize_media_names_to_snake_case.py NAME_NORMALIZED Normalized name from "MO-CFXI MEDIUM" to "mo_cfxi_medium" for consistent matching
2026-06-05T07:41:35.875087+00:00 mim-legacy-to-chebi-migration-v1.0 Refreshed legacy MediaIngredientMech links to CHEBI keying Replaced 31 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme.
2026-06-10T07:37:35.616409+00:00 primary-term-regrounding-v1.0 Re-grounded wrong primary term id (OAK-verified) 1 primary term(s); ingredient name resolved by OAK sqlite:obo:chebi.
2026-06-10T07:47:08.406463+00:00 primary-term-label-canonicalization-v1.0 Canonicalized primary term label to OBO canonical (OAK) 4 primary term(s); ingredient name resolved by OAK sqlite:obo:chebi.