mineral_lactate_medium_low_carbon_content

CultureMech:000840 bacterial DEFINED LIQUID
Original name: MINERAL LACTATE MEDIUM (low carbon content)

Overview

External ontology
mediadive.medium:137 (source link unavailable) — MINERAL LACTATE MEDIUM (low carbon content)
Applications
  • Microbial cultivation
pH
7.0
Notes
Source: DSMZ | Link: https://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium137.pdf

Composition

flowchart LR
    CultureMech_000840["mineral_lactate_medium_low_carbon_content"]
    ing0_Na_lactate["Na-lactate"]
    CultureMech_000840 --> ing0_Na_lactate
    CHEBI_75228["CHEBI:75228"]
    ing0_Na_lactate -.-> CHEBI_75228
    ing1_K2HPO4_x_3_H2O["K2HPO4 x 3 H2O"]
    CultureMech_000840 --> ing1_K2HPO4_x_3_H2O
    CHEBI_131527["CHEBI:131527"]
    ing1_K2HPO4_x_3_H2O -.-> CHEBI_131527
    ing2_KH2PO4["KH2PO4"]
    CultureMech_000840 --> ing2_KH2PO4
    CHEBI_63036["CHEBI:63036"]
    ing2_KH2PO4 -.-> CHEBI_63036
    ing3_NH4Cl["NH4Cl"]
    CultureMech_000840 --> ing3_NH4Cl
    CHEBI_31206["CHEBI:31206"]
    ing3_NH4Cl -.-> CHEBI_31206
    ing4_MgSO4_x_7_H2O["MgSO4 x 7 H2O"]
    CultureMech_000840 --> ing4_MgSO4_x_7_H2O
    CHEBI_31795["CHEBI:31795"]
    ing4_MgSO4_x_7_H2O -.-> CHEBI_31795
    ing5_NaCl["NaCl"]
    CultureMech_000840 --> ing5_NaCl
    CHEBI_26710["CHEBI:26710"]
    ing5_NaCl -.-> CHEBI_26710
    ing6_CaCl2_x_2_H2O["CaCl2 x 2 H2O"]
    CultureMech_000840 --> ing6_CaCl2_x_2_H2O
    CHEBI_86158["CHEBI:86158"]
    ing6_CaCl2_x_2_H2O -.-> CHEBI_86158
    ing7_Na2_EDTA["Na2-EDTA"]
    CultureMech_000840 --> ing7_Na2_EDTA
    CHEBI_64734["CHEBI:64734"]
    ing7_Na2_EDTA -.-> CHEBI_64734
    ing8__NH4_6Mo7O24_x_4_H2O["(NH4)6Mo7O24 x 4 H2O"]
    CultureMech_000840 --> ing8__NH4_6Mo7O24_x_4_H2O
    CHEBI_91249["CHEBI:91249"]
    ing8__NH4_6Mo7O24_x_4_H2O -.-> CHEBI_91249

Ingredients (9)

# Ingredient Concentration Ontology Notes
1 Na-lactate
C3H5O3.Na 112.06 g/mol
0.5 G_PER_L CHEBI:75228
sodium lactate
2 K2HPO4 x 3 H2O
HO4P.2K 174.174 g/mol
1.13 G_PER_L CHEBI:131527
dipotassium hydrogen phosphate
3 KH2PO4
H2O4P.K 136.084 g/mol
0.88 G_PER_L CHEBI:63036
potassium dihydrogen phosphate
4 NH4Cl
Cl.H4N 53.492 g/mol
1 G_PER_L CHEBI:31206
ammonium chloride
5 MgSO4 x 7 H2O
7H2O.Mg.O4S 246.473 g/mol
0.5 G_PER_L CHEBI:31795
magnesium sulfate heptahydrate
6 NaCl
Cl.Na 58.443 g/mol
1 G_PER_L CHEBI:26710
sodium chloride
7 CaCl2 x 2 H2O
Ca.2Cl.2H2O 147.014 g/mol
5.545 G_PER_L CHEBI:86158
calcium chloride dihydrate
 [Merged 2 duplicates: 0.005, 5.54]
8 Na2-EDTA
C10H14N2O8.2Na 336.208 g/mol
50 G_PER_L CHEBI:64734
EDTA disodium salt (anhydrous)
9 (NH4)6Mo7O24 x 4 H2O
2H4N.MoO4 196.014 g/mol
1.1 G_PER_L CHEBI:91249
ammonium molybdate

Preparation

  1. Adjust pH to 7.0.
  2. Dissolve EDTA in distilled water, adjust pH to 7 using 2 N KOH, then add remaining compounds. Adjust pH of final solution to 6.0 with KOH.

Solutions

Trace element solution (Vishniac & Santer, 1957)

Dose: 1.2 ML_PER_L

Stock composition
IngredientConcentrationNotes
ZnSO4 x 7 H2O 22 G_PER_L
MnCl2 x 4 H2O 5.06 G_PER_L
FeSO4 x 7 H2O 5 G_PER_L
CuSO4 x 5 H2O 1.57 G_PER_L
CoCl2 x 6 H2O 1.61 G_PER_L

Target organisms

No target organisms are recorded.

Provenance

TimestampCuratorActionNotes
2026-01-26T17:53:10.292853Z mediadive-import Imported from MediaDive Source: DSMZ, ID: 137
2026-03-13T03:08:56.815902Z data-quality-cleanup-v1.0 Data quality fixes Merged 1 duplicate ingredient(s)
2026-03-13T06:18:58.806167Z culturemech-id-assigner-v1.0 Assigned CultureMech ID Assigned stable identifier: CultureMech:000840
2026-03-17T06:30:01.000000Z mediaingredientmech-enrichment-v1.0 Added MediaIngredientMech links Linked ingredients to MediaIngredientMech identifiers
2026-03-17T19:45:10.260261+00:00 normalize_media_names_to_snake_case.py NAME_NORMALIZED Normalized name from "MINERAL LACTATE MEDIUM (low carbon content)" to "mineral_lactate_medium_low_carbon_content" for consistent matching
2026-06-05T07:41:34.057309+00:00 mim-legacy-to-chebi-migration-v1.0 Refreshed legacy MediaIngredientMech links to CHEBI keying Replaced 13 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme.
2026-06-10T07:47:07.847454+00:00 primary-term-label-canonicalization-v1.0 Canonicalized primary term label to OBO canonical (OAK) 5 primary term(s); ingredient name resolved by OAK sqlite:obo:chebi.
2026-08-07T07:05:22.622702+00:00 apply_cocktail_nesting.py NESTED_FLATTENED_COCKTAIL Moved 5 stock-strength component(s) out of ingredients into 1 solution(s): 5 -> 'Trace element solution (Vishniac & Santer, 1957)' @ 1.2 ml/l. Each moved component matched the MediaDive stock by name AND value; bulk ingredients sharing a name were left in place (#150).