nitrolancetus_medium

CultureMech:000845 bacterial DEFINED LIQUID
Original name: NITROLANCETUS MEDIUM

Overview

External ontology
mediadive.medium:1385 (source link unavailable) — NITROLANCETUS MEDIUM
Applications
  • Microbial cultivation
pH
7.2
Notes
Source: DSMZ | Link: https://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium1385.pdf

Composition

flowchart LR
    CultureMech_000845["nitrolancetus_medium"]
    ing0_KH2PO4["KH2PO4"]
    CultureMech_000845 --> ing0_KH2PO4
    CHEBI_63036["CHEBI:63036"]
    ing0_KH2PO4 -.-> CHEBI_63036
    ing1_CaCl2_x_2_H2O["CaCl2 x 2 H2O"]
    CultureMech_000845 --> ing1_CaCl2_x_2_H2O
    CHEBI_86158["CHEBI:86158"]
    ing1_CaCl2_x_2_H2O -.-> CHEBI_86158
    ing2_MgSO4_x_7_H2O["MgSO4 x 7 H2O"]
    CultureMech_000845 --> ing2_MgSO4_x_7_H2O
    CHEBI_31795["CHEBI:31795"]
    ing2_MgSO4_x_7_H2O -.-> CHEBI_31795
    ing3_NH4HCO3["NH4HCO3"]
    CultureMech_000845 --> ing3_NH4HCO3
    CHEBI_184335["CHEBI:184335"]
    ing3_NH4HCO3 -.-> CHEBI_184335
    ing4_KNO2["KNO2"]
    CultureMech_000845 --> ing4_KNO2
    CHEBI_232610["CHEBI:232610"]
    ing4_KNO2 -.-> CHEBI_232610
    ing5_Sodium_formate["Sodium formate"]
    CultureMech_000845 --> ing5_Sodium_formate
    CHEBI_62965["CHEBI:62965"]
    ing5_Sodium_formate -.-> CHEBI_62965
    ing6_EDTA["EDTA"]
    CultureMech_000845 --> ing6_EDTA
    CHEBI_4735["CHEBI:4735"]
    ing6_EDTA -.-> CHEBI_4735
    ing7_FeSO4_x_7_H2O["FeSO4 x 7 H2O"]
    CultureMech_000845 --> ing7_FeSO4_x_7_H2O
    CHEBI_75836["CHEBI:75836"]
    ing7_FeSO4_x_7_H2O -.-> CHEBI_75836
    ing8_ZnSO4_x_7_H2O["ZnSO4 x 7 H2O"]
    CultureMech_000845 --> ing8_ZnSO4_x_7_H2O
    CHEBI_32312["CHEBI:32312"]
    ing8_ZnSO4_x_7_H2O -.-> CHEBI_32312
    ing9_H3BO3["H3BO3"]
    CultureMech_000845 --> ing9_H3BO3
    CHEBI_33118["CHEBI:33118"]
    ing9_H3BO3 -.-> CHEBI_33118
    ing10_CoCl2_x_6_H2O["CoCl2 x 6 H2O"]
    CultureMech_000845 --> ing10_CoCl2_x_6_H2O
    CHEBI_53503["CHEBI:53503"]
    ing10_CoCl2_x_6_H2O -.-> CHEBI_53503
    ing11_NiCl2_x_6_H2O["NiCl2 x 6 H2O"]
    CultureMech_000845 --> ing11_NiCl2_x_6_H2O
    CHEBI_34887["CHEBI:34887"]
    ing11_NiCl2_x_6_H2O -.-> CHEBI_34887
    ing12_Na2MoO4_x_2_H2O["Na2MoO4 x 2 H2O"]
    CultureMech_000845 --> ing12_Na2MoO4_x_2_H2O
    CHEBI_75213["CHEBI:75213"]
    ing12_Na2MoO4_x_2_H2O -.-> CHEBI_75213
    ing13_MnCl2_x_4_H2O["MnCl2 x 4 H2O"]
    CultureMech_000845 --> ing13_MnCl2_x_4_H2O
    CHEBI_86368["CHEBI:86368"]
    ing13_MnCl2_x_4_H2O -.-> CHEBI_86368
    ing14_CuCl2_x_2_H2O["CuCl2 x 2 H2O"]
    CultureMech_000845 --> ing14_CuCl2_x_2_H2O
    CHEBI_86318["CHEBI:86318"]
    ing14_CuCl2_x_2_H2O -.-> CHEBI_86318

Ingredients (15)

# Ingredient Concentration Ontology Notes
1 KH2PO4
H2O4P.K 136.084 g/mol
0.15 G_PER_L CHEBI:63036
potassium dihydrogen phosphate
2 CaCl2 x 2 H2O
Ca.2Cl.2H2O 147.014 g/mol
0.05 G_PER_L CHEBI:86158
calcium chloride dihydrate
3 MgSO4 x 7 H2O
7H2O.Mg.O4S 246.473 g/mol
0.26 G_PER_L CHEBI:31795
magnesium sulfate heptahydrate
4 NH4HCO3
CHO3.H4N 79.055 g/mol
0.395 G_PER_L CHEBI:184335
Ammonium bicarbonate
5 KNO2
K.NO2 85.103 g/mol
4.25 G_PER_L CHEBI:232610
potassium nitrite
6 Sodium formate
CHO2.Na 68.007 g/mol
2.72 G_PER_L CHEBI:62965
sodium formate
7 EDTA
C10H16N2O8 292.244 g/mol
0.5 G_PER_L CHEBI:4735
ethylenediaminetetraacetic acid
8 FeSO4 x 7 H2O
Fe.7H2O.O4S 278.013 g/mol
0.2 G_PER_L CHEBI:75836
iron(2+) sulfate heptahydrate
9 ZnSO4 x 7 H2O
7H2O.O4S.Zn 287.558 g/mol
0.01 G_PER_L CHEBI:32312
zinc sulfate heptahydrate
10 H3BO3
H3BO3 61.833 g/mol
0.03 G_PER_L CHEBI:33118
boric acid
11 CoCl2 x 6 H2O
2Cl.Co.6H2O 237.929 g/mol
0.02 G_PER_L CHEBI:53503
cobalt chloride hexahydrate
12 NiCl2 x 6 H2O
Cl2Ni 129.599 g/mol
0.002 G_PER_L CHEBI:34887
nickel dichloride
13 Na2MoO4 x 2 H2O
2H2O.MoO4.2Na 241.946 g/mol
0.003 G_PER_L CHEBI:75213
sodium molybdate dihydrate
14 MnCl2 x 4 H2O
Cl2Mn.4H2O 197.904 g/mol
0.003 G_PER_L CHEBI:86368
manganese(II) chloride tetrahydrate
15 CuCl2 x 2 H2O
Cl2Cu.2H2O 170.482 g/mol
0.001 G_PER_L CHEBI:86318
copper(II) chloride dihydrate

Preparation

  1. Prepare the liquid medium. Adjust to pH 7.2. Dispense the medium into serum bottles. Inject 10 % CO2. Sterilize by autoclaving. Before inoculation add from sterile stock solutions:

Target organisms

No target organisms are recorded.

Provenance

TimestampCuratorActionNotes
2026-01-26T17:53:14.270297Z mediadive-import Imported from MediaDive Source: DSMZ, ID: 1385
2026-01-28T19:48:28.480089Z chebi-enrichment Added CHEBI ontology terms to ingredients Enriched using MicrobeMediaParam and MediaDive chemical mappings
2026-03-13T06:18:58.846747Z culturemech-id-assigner-v1.0 Assigned CultureMech ID Assigned stable identifier: CultureMech:000845
2026-03-17T06:30:25.000000Z mediaingredientmech-enrichment-v1.0 Added MediaIngredientMech links Linked ingredients to MediaIngredientMech identifiers
2026-03-17T19:45:10.305280+00:00 normalize_media_names_to_snake_case.py NAME_NORMALIZED Normalized name from "NITROLANCETUS MEDIUM" to "nitrolancetus_medium" for consistent matching
2026-06-05T07:41:39.812480+00:00 mim-legacy-to-chebi-migration-v1.0 Refreshed legacy MediaIngredientMech links to CHEBI keying Replaced 14 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme.
2026-06-10T07:47:09.597545+00:00 primary-term-label-canonicalization-v1.0 Canonicalized primary term label to OBO canonical (OAK) 2 primary term(s); ingredient name resolved by OAK sqlite:obo:chebi.
2026-08-20T19:03:00.135028+00:00 apply_mim_groundings.py Adopted MIM published grounding Grounded 1 ingredient(s) from MIM's ingredient_mappings.sssom.tsv (2026-08-18), exactMatch only, where this record carried no CHEBI term (#308).