rhodovulum_medium

CultureMech:000851 bacterial COMPLEX LIQUID
Original name: RHODOVULUM MEDIUM

Overview

External ontology
mediadive.medium:1390 (source link unavailable) — RHODOVULUM MEDIUM
Applications
  • Microbial cultivation
pH
7.0
Notes
Source: DSMZ | Link: https://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium1390.pdf

Composition

flowchart LR
    CultureMech_000851["rhodovulum_medium"]
    ing0_Yeast_extract["Yeast extract"]
    CultureMech_000851 --> ing0_Yeast_extract
    ing1_KH2PO4["KH2PO4"]
    CultureMech_000851 --> ing1_KH2PO4
    CHEBI_63036["CHEBI:63036"]
    ing1_KH2PO4 -.-> CHEBI_63036
    ing2_NH4Cl["NH4Cl"]
    CultureMech_000851 --> ing2_NH4Cl
    CHEBI_31206["CHEBI:31206"]
    ing2_NH4Cl -.-> CHEBI_31206
    ing3_Na2SO4["Na2SO4"]
    CultureMech_000851 --> ing3_Na2SO4
    CHEBI_32149["CHEBI:32149"]
    ing3_Na2SO4 -.-> CHEBI_32149
    ing4_NaCl["NaCl"]
    CultureMech_000851 --> ing4_NaCl
    CHEBI_26710["CHEBI:26710"]
    ing4_NaCl -.-> CHEBI_26710
    ing5_NaHCO3["NaHCO3"]
    CultureMech_000851 --> ing5_NaHCO3
    CHEBI_32139["CHEBI:32139"]
    ing5_NaHCO3 -.-> CHEBI_32139
    ing6_Na2S_x_9_H2O["Na2S x 9 H2O"]
    CultureMech_000851 --> ing6_Na2S_x_9_H2O
    CHEBI_76209["CHEBI:76209"]
    ing6_Na2S_x_9_H2O -.-> CHEBI_76209
    ing7_MgCl2_x_6_H2O["MgCl2 x 6 H2O"]
    CultureMech_000851 --> ing7_MgCl2_x_6_H2O
    CHEBI_86345["CHEBI:86345"]
    ing7_MgCl2_x_6_H2O -.-> CHEBI_86345
    ing8_CaCl2_x_2_H2O["CaCl2 x 2 H2O"]
    CultureMech_000851 --> ing8_CaCl2_x_2_H2O
    CHEBI_86158["CHEBI:86158"]
    ing8_CaCl2_x_2_H2O -.-> CHEBI_86158
    ing9_Na_ascorbate["Na-ascorbate"]
    CultureMech_000851 --> ing9_Na_ascorbate
    CHEBI_113451["CHEBI:113451"]
    ing9_Na_ascorbate -.-> CHEBI_113451
    ing10_Na_malate["Na-malate"]
    CultureMech_000851 --> ing10_Na_malate
    CHEBI_91260["CHEBI:91260"]
    ing10_Na_malate -.-> CHEBI_91260
    ing11_Vitamin_B12["Vitamin B12"]
    CultureMech_000851 --> ing11_Vitamin_B12
    CHEBI_176843["CHEBI:176843"]
    ing11_Vitamin_B12 -.-> CHEBI_176843
    ing12_CoCl2_x_6_H2O["CoCl2 x 6 H2O"]
    CultureMech_000851 --> ing12_CoCl2_x_6_H2O
    CHEBI_53503["CHEBI:53503"]
    ing12_CoCl2_x_6_H2O -.-> CHEBI_53503
    ing13_NiCl2_x_6_H2O["NiCl2 x 6 H2O"]
    CultureMech_000851 --> ing13_NiCl2_x_6_H2O
    CHEBI_34887["CHEBI:34887"]
    ing13_NiCl2_x_6_H2O -.-> CHEBI_34887
    ing14_CuCl2_x_2_H2O["CuCl2 x 2 H2O"]
    CultureMech_000851 --> ing14_CuCl2_x_2_H2O
    CHEBI_86318["CHEBI:86318"]
    ing14_CuCl2_x_2_H2O -.-> CHEBI_86318
    ing15_MnCl2_x_4_H2O["MnCl2 x 4 H2O"]
    CultureMech_000851 --> ing15_MnCl2_x_4_H2O
    CHEBI_86368["CHEBI:86368"]
    ing15_MnCl2_x_4_H2O -.-> CHEBI_86368
    ing16_ZnCl2["ZnCl2"]
    CultureMech_000851 --> ing16_ZnCl2
    CHEBI_49976["CHEBI:49976"]
    ing16_ZnCl2 -.-> CHEBI_49976
    ing17_H3BO3["H3BO3"]
    CultureMech_000851 --> ing17_H3BO3
    CHEBI_33118["CHEBI:33118"]
    ing17_H3BO3 -.-> CHEBI_33118
    ing18_Na2MoO4_x_2_H2O["Na2MoO4 x 2 H2O"]
    CultureMech_000851 --> ing18_Na2MoO4_x_2_H2O
    CHEBI_75213["CHEBI:75213"]
    ing18_Na2MoO4_x_2_H2O -.-> CHEBI_75213
    ing19_Na2SeO3_x_5_H2O["Na2SeO3 x 5 H2O"]
    CultureMech_000851 --> ing19_Na2SeO3_x_5_H2O
    CHEBI_131361["CHEBI:131361"]
    ing19_Na2SeO3_x_5_H2O -.-> CHEBI_131361
    ing20_Pyridoxal_hydrochloride["Pyridoxal hydrochloride"]
    CultureMech_000851 --> ing20_Pyridoxal_hydrochloride
    CHEBI_131529["CHEBI:131529"]
    ing20_Pyridoxal_hydrochloride -.-> CHEBI_131529
    ing21_Calcium_pantothenate["Calcium pantothenate"]
    CultureMech_000851 --> ing21_Calcium_pantothenate
    CHEBI_31345["CHEBI:31345"]
    ing21_Calcium_pantothenate -.-> CHEBI_31345

Ingredients (22)

# Ingredient Concentration Ontology Notes
1 Yeast extract 0.1 G_PER_L
2 KH2PO4
H2O4P.K 136.084 g/mol
1 G_PER_L CHEBI:63036
potassium dihydrogen phosphate
3 NH4Cl
Cl.H4N 53.492 g/mol
1 G_PER_L CHEBI:31206
ammonium chloride
4 Na2SO4
2Na.O4S 142.043 g/mol
0.7 G_PER_L CHEBI:32149
sodium sulfate
5 NaCl
Cl.Na 58.443 g/mol
120 G_PER_L CHEBI:26710
sodium chloride
6 NaHCO3
CHO3.Na 84.006 g/mol
3 G_PER_L CHEBI:32139
sodium hydrogencarbonate
7 Na2S x 9 H2O
9H2O.2Na.S 240.182 g/mol
0.2 G_PER_L CHEBI:76209
sodium sulfide nonahydrate
8 MgCl2 x 6 H2O
2Cl.6H2O.Mg 203.301 g/mol
0.5 G_PER_L CHEBI:86345
magnesium dichloride hexahydrate
9 CaCl2 x 2 H2O
Ca.2Cl.2H2O 147.014 g/mol
0.1 G_PER_L CHEBI:86158
calcium chloride dihydrate
10 Na-ascorbate
C6H7O6.Na 198.106 g/mol
0.5 G_PER_L CHEBI:113451
sodium ascorbate
11 Na-malate 1 G_PER_L CHEBI:91260
disodium malate
12 Vitamin B12 0.05004 G_PER_L CHEBI:176843
vitamin B12
 [Merged 3 duplicates: 2e-05, 2e-05, 0.05]
13 CoCl2 x 6 H2O
2Cl.Co.6H2O 237.929 g/mol
0.25 G_PER_L CHEBI:53503
cobalt chloride hexahydrate
14 NiCl2 x 6 H2O
Cl2Ni 129.599 g/mol
0.01 G_PER_L CHEBI:34887
nickel dichloride
15 CuCl2 x 2 H2O
Cl2Cu.2H2O 170.482 g/mol
0.01 G_PER_L CHEBI:86318
copper(II) chloride dihydrate
16 MnCl2 x 4 H2O
Cl2Mn.4H2O 197.904 g/mol
0.07 G_PER_L CHEBI:86368
manganese(II) chloride tetrahydrate
17 ZnCl2
2Cl.Zn 136.296 g/mol
0.1 G_PER_L CHEBI:49976
zinc dichloride
18 H3BO3
H3BO3 61.833 g/mol
0.5 G_PER_L CHEBI:33118
boric acid
19 Na2MoO4 x 2 H2O
2H2O.MoO4.2Na 241.946 g/mol
0.03 G_PER_L CHEBI:75213
sodium molybdate dihydrate
20 Na2SeO3 x 5 H2O
5H2O.2Na.O3Se 263.012 g/mol
0.01 G_PER_L CHEBI:131361
disodium selenite pentahydrate
21 Pyridoxal hydrochloride
C8H9NO3.HCl 203.625 g/mol
0.1 G_PER_L CHEBI:131529
pyridoxal hydrochloride
22 Calcium pantothenate
2C9H16NO5.Ca 476.536 g/mol
0.1 G_PER_L CHEBI:31345
Calcium pantothenate

Preparation

  1. Adjust ph to 7.0
  2. Prepare the medium under N2 gas without the NaHCO3, Na2S x 9 H2O, VA-Vitamin solution and vitamin B12. Autoclave and add the VA-Vitamin solution and vitamin B12 from a filter-sterilised stock solution and the NaHCO3, Na2S x 9 H2O, from sterile stock solutions. The final pH of the medium should be 7.0.
  3. Adjust pH to 2.0 - 3.0.

Solutions

Trace element solution (SLA)

Dose: 1 ML_PER_L

Stock composition
IngredientConcentrationNotes
FeCl2 x 4 H2O 1.8 G_PER_L

Vitamin solution (VA)

Dose: 1 ML_PER_L

Stock composition
IngredientConcentrationNotes
Biotin 0.1 G_PER_L
Nicotinic acid amide 0.35 G_PER_L
Thiamine-HCl x 2 H2O 0.3 G_PER_L
p-Aminobenzoic acid 0.2 G_PER_L

Target organisms

No target organisms are recorded.

Provenance

TimestampCuratorActionNotes
2026-01-26T17:53:14.283557Z mediadive-import Imported from MediaDive Source: DSMZ, ID: 1390
2026-03-13T03:08:39.406104Z data-quality-cleanup-v1.0 Data quality fixes Merged 2 duplicate ingredient(s)
2026-03-13T06:18:58.892512Z culturemech-id-assigner-v1.0 Assigned CultureMech ID Assigned stable identifier: CultureMech:000851
2026-03-17T06:29:41.000000Z mediaingredientmech-enrichment-v1.0 Added MediaIngredientMech links Linked ingredients to MediaIngredientMech identifiers
2026-03-17T19:45:10.354641+00:00 normalize_media_names_to_snake_case.py NAME_NORMALIZED Normalized name from "RHODOVULUM MEDIUM" to "rhodovulum_medium" for consistent matching
2026-06-05T07:41:44.519459+00:00 mim-legacy-to-chebi-migration-v1.0 Refreshed legacy MediaIngredientMech links to CHEBI keying Replaced 26 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme.
2026-06-10T07:47:11.029565+00:00 primary-term-label-canonicalization-v1.0 Canonicalized primary term label to OBO canonical (OAK) 5 primary term(s); ingredient name resolved by OAK sqlite:obo:chebi.
2026-08-07T07:05:22.820523+00:00 apply_cocktail_nesting.py NESTED_FLATTENED_COCKTAIL Moved 5 stock-strength component(s) out of ingredients into 2 solution(s): 1 -> 'Trace element solution (SLA)' @ 1 ml/l; 4 -> 'Vitamin solution (VA)' @ 1 ml/l. Each moved component matched the MediaDive stock by name AND value; bulk ingredients sharing a name were left in place (#150).