Overview
- External ontology
- mediadive.medium:1394 (source link unavailable) — R2A MEDIUM WITH SEAWATER 50%
- Applications
-
- Microbial cultivation
- pH
- 7.0
- Notes
- Source: DSMZ | Link: https://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium1394.pdf
Composition
flowchart LR
CultureMech_000855["r2a_medium_with_seawater_50"]
ing0_Yeast_extract["Yeast extract"]
CultureMech_000855 --> ing0_Yeast_extract
ing1_Proteose_peptone["Proteose peptone"]
CultureMech_000855 --> ing1_Proteose_peptone
ing2_Casamino_acids["Casamino acids"]
CultureMech_000855 --> ing2_Casamino_acids
ing3_Glucose["Glucose"]
CultureMech_000855 --> ing3_Glucose
CHEBI_17234["CHEBI:17234"]
ing3_Glucose -.-> CHEBI_17234
ing4_Starch["Starch"]
CultureMech_000855 --> ing4_Starch
CHEBI_28017["CHEBI:28017"]
ing4_Starch -.-> CHEBI_28017
ing5_Na_pyruvate["Na-pyruvate"]
CultureMech_000855 --> ing5_Na_pyruvate
CHEBI_50144["CHEBI:50144"]
ing5_Na_pyruvate -.-> CHEBI_50144
ing6_K2HPO4["K2HPO4"]
CultureMech_000855 --> ing6_K2HPO4
CHEBI_131527["CHEBI:131527"]
ing6_K2HPO4 -.-> CHEBI_131527
ing7_MgSO4_x_7_H2O["MgSO4 x 7 H2O"]
CultureMech_000855 --> ing7_MgSO4_x_7_H2O
CHEBI_31795["CHEBI:31795"]
ing7_MgSO4_x_7_H2O -.-> CHEBI_31795
ing8_FeSO4["FeSO4"]
CultureMech_000855 --> ing8_FeSO4
CHEBI_75832["CHEBI:75832"]
ing8_FeSO4 -.-> CHEBI_75832
ing9_Sea_water["Sea water"]
CultureMech_000855 --> ing9_Sea_water
ing10_Agar["Agar"]
CultureMech_000855 --> ing10_Agar
CHEBI_2509["CHEBI:2509"]
ing10_Agar -.-> CHEBI_2509
Ingredients (11)
| # | Ingredient | Concentration | Ontology | Notes |
|---|---|---|---|---|
| 1 | Yeast extract | 0.5 G_PER_L | ||
| 2 | Proteose peptone | 0.5 G_PER_L | ||
| 3 | Casamino acids | 0.5 G_PER_L | ||
| 4 |
Glucose
C6H12O6 180.156 g/mol
|
0.5 G_PER_L |
CHEBI:17234
glucose
|
|
| 5 | Starch | 0.5 G_PER_L |
CHEBI:28017
starch
|
|
| 6 |
Na-pyruvate
C3H3O3.Na 110.044 g/mol
|
0.3 G_PER_L |
CHEBI:50144
sodium pyruvate
|
|
| 7 |
K2HPO4
HO4P.2K 174.174 g/mol
|
0.3 G_PER_L |
CHEBI:131527
dipotassium hydrogen phosphate
|
|
| 8 |
MgSO4 x 7 H2O
7H2O.Mg.O4S 246.473 g/mol
|
0.05 G_PER_L |
CHEBI:31795
magnesium sulfate heptahydrate
|
|
| 9 |
FeSO4
Fe.O4S 151.908 g/mol
|
0.001 G_PER_L |
CHEBI:75832
iron(2+) sulfate (anhydrous)
|
|
| 10 | Sea water | 500 G_PER_L | ||
| 11 | Agar | 20 G_PER_L |
CHEBI:2509
agar
|
(for solid medium) |
Preparation
- Adjust to pH 7.0 and sterilize by autoclaving. The medium may be solidified by adding 20.0 g/l agar.
Target organisms
No target organisms are recorded.
Provenance
| Timestamp | Curator | Action | Notes |
|---|---|---|---|
| 2026-01-26T17:53:14.296348Z | mediadive-import | Imported from MediaDive | Source: DSMZ, ID: 1394 |
| 2026-01-28T19:48:11.124545Z | chebi-enrichment | Added CHEBI ontology terms to ingredients | Enriched using MicrobeMediaParam and MediaDive chemical mappings |
| 2026-03-13T06:18:58.929505Z | culturemech-id-assigner-v1.0 | Assigned CultureMech ID | Assigned stable identifier: CultureMech:000855 |
| 2026-03-17T06:29:48.000000Z | mediaingredientmech-enrichment-v1.0 | Added MediaIngredientMech links | Linked ingredients to MediaIngredientMech identifiers |
| 2026-03-17T19:45:10.392080+00:00 | normalize_media_names_to_snake_case.py | NAME_NORMALIZED | Normalized name from "R2A MEDIUM WITH SEAWATER 50%" to "r2a_medium_with_seawater_50" for consistent matching |
| 2026-06-05T07:41:43.656608+00:00 | mim-legacy-to-chebi-migration-v1.0 | Refreshed legacy MediaIngredientMech links to CHEBI keying | Replaced 8 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme. |
| 2026-06-08T23:19:50.038271+00:00 | chebi-regrounding-v1.0 | Corrected CHEBI ingredient grounding (audit G21/G22) | Re-grounded 2 term reference(s) to the correct CHEBI (see reports/chebi_grounding_audit.md). Label-conditional fixes for pyridoxine/pyridoxamine, glycerol, MnSO4, Ca(NO3)2, selenate; casamino acids de-grounded (mixture, no single CHEBI). |
| 2026-06-10T07:47:10.792010+00:00 | primary-term-label-canonicalization-v1.0 | Canonicalized primary term label to OBO canonical (OAK) | 2 primary term(s); ingredient name resolved by OAK sqlite:obo:chebi. |