haloarchaeal_medium_mh_1

CultureMech:000857 archaea COMPLEX SOLID_AGAR
Original name: HALOARCHAEAL MEDIUM MH-1

Overview

External ontology
mediadive.medium:1396 (source link unavailable) — HALOARCHAEAL MEDIUM MH-1
Applications
  • Microbial cultivation
pH
4.5
High-metal flag
True
Notes
Source: DSMZ | Link: https://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium1396.pdf

Composition

flowchart LR
    CultureMech_000857["haloarchaeal_medium_mh_1"]
    ing0_L_Glutamic_acid["L-Glutamic acid"]
    CultureMech_000857 --> ing0_L_Glutamic_acid
    CHEBI_16015["CHEBI:16015"]
    ing0_L_Glutamic_acid -.-> CHEBI_16015
    ing1_Trisodium_citrate_x_2_H2O["Trisodium citrate x 2 H2O"]
    CultureMech_000857 --> ing1_Trisodium_citrate_x_2_H2O
    CHEBI_53258["CHEBI:53258"]
    ing1_Trisodium_citrate_x_2_H2O -.-> CHEBI_53258
    ing2_KH2PO4["KH2PO4"]
    CultureMech_000857 --> ing2_KH2PO4
    CHEBI_63036["CHEBI:63036"]
    ing2_KH2PO4 -.-> CHEBI_63036
    ing3_NH4Cl["NH4Cl"]
    CultureMech_000857 --> ing3_NH4Cl
    CHEBI_31206["CHEBI:31206"]
    ing3_NH4Cl -.-> CHEBI_31206
    ing4_K2SO4["K2SO4"]
    CultureMech_000857 --> ing4_K2SO4
    CHEBI_32036["CHEBI:32036"]
    ing4_K2SO4 -.-> CHEBI_32036
    ing5_NaCl["NaCl"]
    CultureMech_000857 --> ing5_NaCl
    CHEBI_26710["CHEBI:26710"]
    ing5_NaCl -.-> CHEBI_26710
    ing6_Casamino_acids["Casamino acids"]
    CultureMech_000857 --> ing6_Casamino_acids
    ing7_Yeast_extract["Yeast extract"]
    CultureMech_000857 --> ing7_Yeast_extract
    ing8_FeSO4_x_6_H2O["FeSO4 x 6 H2O"]
    CultureMech_000857 --> ing8_FeSO4_x_6_H2O
    CHEBI_75832["CHEBI:75832"]
    ing8_FeSO4_x_6_H2O -.-> CHEBI_75832
    ing9_Agar["Agar"]
    CultureMech_000857 --> ing9_Agar
    CHEBI_2509["CHEBI:2509"]
    ing9_Agar -.-> CHEBI_2509
    ing10_CoCl2_x_6_H2O["CoCl2 x 6 H2O"]
    CultureMech_000857 --> ing10_CoCl2_x_6_H2O
    CHEBI_53503["CHEBI:53503"]
    ing10_CoCl2_x_6_H2O -.-> CHEBI_53503
    ing11_MnCl2_x_4_H2O["MnCl2 x 4 H2O"]
    CultureMech_000857 --> ing11_MnCl2_x_4_H2O
    CHEBI_86368["CHEBI:86368"]
    ing11_MnCl2_x_4_H2O -.-> CHEBI_86368
    ing12_ZnCl2["ZnCl2"]
    CultureMech_000857 --> ing12_ZnCl2
    CHEBI_49976["CHEBI:49976"]
    ing12_ZnCl2 -.-> CHEBI_49976
    ing13_H3BO4["H3BO4"]
    CultureMech_000857 --> ing13_H3BO4
    ing14_Na2MoO4_x_2_H2O["Na2MoO4 x 2 H2O"]
    CultureMech_000857 --> ing14_Na2MoO4_x_2_H2O
    CHEBI_75213["CHEBI:75213"]
    ing14_Na2MoO4_x_2_H2O -.-> CHEBI_75213
    ing15_NiCl2_x_6_H2O["NiCl2 x 6 H2O"]
    CultureMech_000857 --> ing15_NiCl2_x_6_H2O
    CHEBI_34887["CHEBI:34887"]
    ing15_NiCl2_x_6_H2O -.-> CHEBI_34887
    ing16_CuCl2_x_6_H2O["CuCl2 x 6 H2O"]
    CultureMech_000857 --> ing16_CuCl2_x_6_H2O
    CHEBI_49553["CHEBI:49553"]
    ing16_CuCl2_x_6_H2O -.-> CHEBI_49553
    ing17_AlCl3["AlCl3"]
    CultureMech_000857 --> ing17_AlCl3
    CHEBI_30114["CHEBI:30114"]
    ing17_AlCl3 -.-> CHEBI_30114
    ing18_Na2WO4_x_2_H2O["Na2WO4 x 2 H2O"]
    CultureMech_000857 --> ing18_Na2WO4_x_2_H2O
    CHEBI_63939["CHEBI:63939"]
    ing18_Na2WO4_x_2_H2O -.-> CHEBI_63939
    ing19_CaCl2_x_2_H2O["CaCl2 x 2 H2O"]
    CultureMech_000857 --> ing19_CaCl2_x_2_H2O
    CHEBI_86158["CHEBI:86158"]
    ing19_CaCl2_x_2_H2O -.-> CHEBI_86158
    ing20_BaCl2_x_2_H2O["BaCl2 x 2 H2O"]
    CultureMech_000857 --> ing20_BaCl2_x_2_H2O
    CHEBI_86153["CHEBI:86153"]
    ing20_BaCl2_x_2_H2O -.-> CHEBI_86153
    ing21_Na2S2O3_x_5_H2O["Na2S2O3 x 5 H2O"]
    CultureMech_000857 --> ing21_Na2S2O3_x_5_H2O
    CHEBI_32150["CHEBI:32150"]
    ing21_Na2S2O3_x_5_H2O -.-> CHEBI_32150

Ingredients (22)

# Ingredient Concentration Ontology Notes
1 L-Glutamic acid
C5H9NO4 147.13 g/mol
2.49377 G_PER_L CHEBI:16015
L-glutamic acid
2 Trisodium citrate x 2 H2O
C6H5O7.3Na 258.069 g/mol
2.49377 G_PER_L CHEBI:53258
sodium citrate
3 KH2PO4
H2O4P.K 136.084 g/mol
1.24688 G_PER_L CHEBI:63036
potassium dihydrogen phosphate
4 NH4Cl
Cl.H4N 53.492 g/mol
1.24688 G_PER_L CHEBI:31206
ammonium chloride
5 K2SO4
2K.O4S 174.259 g/mol
6.23441 G_PER_L CHEBI:32036
potassium sulfate
6 NaCl
Cl.Na 58.443 g/mol
249.377 G_PER_L CHEBI:26710
sodium chloride
7 Casamino acids 4.98753 G_PER_L
8 Yeast extract 2.49377 G_PER_L
9 FeSO4 x 6 H2O
Fe.O4S 151.908 g/mol
0.00498753 G_PER_L CHEBI:75832
iron(2+) sulfate (anhydrous)
10 Agar 24.9377 G_PER_L CHEBI:2509
agar
11 CoCl2 x 6 H2O
2Cl.Co.6H2O 237.929 g/mol
0.3 G_PER_L CHEBI:53503
cobalt chloride hexahydrate
12 MnCl2 x 4 H2O
Cl2Mn.4H2O 197.904 g/mol
0.1 G_PER_L CHEBI:86368
manganese(II) chloride tetrahydrate
13 ZnCl2
2Cl.Zn 136.296 g/mol
0.1 G_PER_L CHEBI:49976
zinc dichloride
14 H3BO4 0.02 G_PER_L
15 Na2MoO4 x 2 H2O
2H2O.MoO4.2Na 241.946 g/mol
0.1 G_PER_L CHEBI:75213
sodium molybdate dihydrate
16 NiCl2 x 6 H2O
Cl2Ni 129.599 g/mol
0.1 G_PER_L CHEBI:34887
nickel dichloride
17 CuCl2 x 6 H2O
Cl2Cu 134.452 g/mol
0.01 G_PER_L CHEBI:49553
copper(II) chloride
18 AlCl3
AlCl3 133.341 g/mol
0.04 G_PER_L CHEBI:30114
aluminium trichloride
19 Na2WO4 x 2 H2O
2H2O.2Na.O4W 329.846 g/mol
0.02 G_PER_L CHEBI:63939
sodium tungstate dihydrate
20 CaCl2 x 2 H2O
Ca.2Cl.2H2O 147.014 g/mol
1 G_PER_L CHEBI:86158
calcium chloride dihydrate
21 BaCl2 x 2 H2O
Ba.2Cl.2H2O 244.264 g/mol
0.01 G_PER_L CHEBI:86153
barium chloride dihydrate
22 Na2S2O3 x 5 H2O
5H2O.2Na.O3S2 248.186 g/mol
2 G_PER_L CHEBI:32150
sodium thiosulfate pentahydrate

Preparation

  1. Adjust to pH 4.5 with 40% KOH and sterilize by autoclaving.
  2. Adjust to pH 4.0.

Target organisms

No target organisms are recorded.

Provenance

TimestampCuratorActionNotes
2026-01-26T17:53:14.300524Z mediadive-import Imported from MediaDive Source: DSMZ, ID: 1396
2026-01-28T19:48:09.637340Z chebi-enrichment Added CHEBI ontology terms to ingredients Enriched using MicrobeMediaParam and MediaDive chemical mappings
2026-03-13T06:18:58.944561Z culturemech-id-assigner-v1.0 Assigned CultureMech ID Assigned stable identifier: CultureMech:000857
2026-03-17T06:29:45.000000Z mediaingredientmech-enrichment-v1.0 Added MediaIngredientMech links Linked ingredients to MediaIngredientMech identifiers
2026-03-17T19:45:10.407692+00:00 normalize_media_names_to_snake_case.py NAME_NORMALIZED Normalized name from "HALOARCHAEAL MEDIUM MH-1" to "haloarchaeal_medium_mh_1" for consistent matching
2026-06-05T07:41:07.472960+00:00 mim-legacy-to-chebi-migration-v1.0 Refreshed legacy MediaIngredientMech links to CHEBI keying Replaced 20 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme.
2026-06-08T23:19:39.583880+00:00 chebi-regrounding-v1.0 Corrected CHEBI ingredient grounding (shared-id regrounding audit, G21/G22/G24) Part of the shared-id CHEBI regrounding audit (G21/G22/G24): one or more ingredient groundings on this record that had been collapsed onto a chemically non-specific or shared CHEBI id were re-grounded to the correct chemically-specific term, verified against OAK chebi.db rdfs:label. See reports/chebi_grounding_audit.md.
2026-06-10T07:46:59.432769+00:00 primary-term-label-canonicalization-v1.0 Canonicalized primary term label to OBO canonical (OAK) 4 primary term(s); ingredient name resolved by OAK sqlite:obo:chebi.