bacillus_acidocaldarius_medium

CultureMech:000861 bacterial COMPLEX SOLID_AGAR
Original name: BACILLUS ACIDOCALDARIUS MEDIUM

Overview

External ontology
mediadive.medium:13 (source link unavailable) — BACILLUS ACIDOCALDARIUS MEDIUM
Applications
  • Microbial cultivation
Notes
Source: DSMZ | Link: https://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium13.pdf

Ingredients (0)

No ingredients recorded.

Preparation

  1. Sterilize solution A and B separately at 121°C for 15 min. Cool to 50°C and then combine.

Solutions

Solution A

Dose: 0.5 L

Stock composition
IngredientConcentrationNotes
Yeast extract 2 G_PER_L
(NH4)2SO4 0.4 G_PER_L
MgSO4 x 7 H2O 1 G_PER_L
CaCl2 x 2 H2O 0.5 G_PER_L
KH2PO4 1.2 G_PER_L
Distilled water 0.5 L

Solution B

Dose: 0.5 L

Stock composition
IngredientConcentrationNotes
Glucose 2 G_PER_L
Agar 40 G_PER_L
Distilled water 0.5 L

Target organisms

No target organisms are recorded.

Provenance

TimestampCuratorActionNotes
2026-01-26T17:53:09.980173Z mediadive-import Imported from MediaDive Source: DSMZ, ID: 13
2026-03-13T06:18:58.981326Z culturemech-id-assigner-v1.0 Assigned CultureMech ID Assigned stable identifier: CultureMech:000861
2026-03-17T06:30:36.000000Z mediaingredientmech-enrichment-v1.0 Added MediaIngredientMech links Linked ingredients to MediaIngredientMech identifiers
2026-03-17T19:45:10.447297+00:00 normalize_media_names_to_snake_case.py NAME_NORMALIZED Normalized name from "BACILLUS ACIDOCALDARIUS MEDIUM" to "bacillus_acidocaldarius_medium" for consistent matching
2026-06-05T07:40:50.222357+00:00 mim-legacy-to-chebi-migration-v1.0 Refreshed legacy MediaIngredientMech links to CHEBI keying Replaced 6 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme.
2026-06-10T07:46:53.698082+00:00 primary-term-label-canonicalization-v1.0 Canonicalized primary term label to OBO canonical (OAK) 2 primary term(s); ingredient name resolved by OAK sqlite:obo:chebi.
2026-10-07T11:55:39.847156+00:00 codex RESTORED_REVIEWED_MEDIUM_STOCK_BOUNDARIES Exact source graph, complete ingredient inventory, amounts, and original PDF recipe chain checked. Numeric concentrations and chemical mappings preserved; stock doses and water are explicit batch amounts. No final-medium stock dilution inferred. Parent/variant relationships remain separately unreviewed.