Overview
- External ontology
- mediadive.medium:1403 (source link unavailable) — METHYLOROSULA MEDIUM V-022
- Applications
-
- Microbial cultivation
- pH
- 6.0
- Notes
- Source: DSMZ | Link: https://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium1403.pdf
Composition
flowchart LR
CultureMech_000864["methylorosula_medium_v_022"]
ing0__NH4_2SO4["(NH4)2SO4"]
CultureMech_000864 --> ing0__NH4_2SO4
CHEBI_62946["CHEBI:62946"]
ing0__NH4_2SO4 -.-> CHEBI_62946
ing1_MgCl2_x_6_H2O["MgCl2 x 6 H2O"]
CultureMech_000864 --> ing1_MgCl2_x_6_H2O
CHEBI_86345["CHEBI:86345"]
ing1_MgCl2_x_6_H2O -.-> CHEBI_86345
ing2_KH2PO4["KH2PO4"]
CultureMech_000864 --> ing2_KH2PO4
CHEBI_63036["CHEBI:63036"]
ing2_KH2PO4 -.-> CHEBI_63036
ing3_Methanol["Methanol"]
CultureMech_000864 --> ing3_Methanol
CHEBI_17790["CHEBI:17790"]
ing3_Methanol -.-> CHEBI_17790
ing4_Na2_EDTA["Na2-EDTA"]
CultureMech_000864 --> ing4_Na2_EDTA
CHEBI_64734["CHEBI:64734"]
ing4_Na2_EDTA -.-> CHEBI_64734
ing5_FeSO4_x_7_H2O["FeSO4 x 7 H2O"]
CultureMech_000864 --> ing5_FeSO4_x_7_H2O
CHEBI_75836["CHEBI:75836"]
ing5_FeSO4_x_7_H2O -.-> CHEBI_75836
ing6_ZnSO4_x_7_H2O["ZnSO4 x 7 H2O"]
CultureMech_000864 --> ing6_ZnSO4_x_7_H2O
CHEBI_32312["CHEBI:32312"]
ing6_ZnSO4_x_7_H2O -.-> CHEBI_32312
ing7_H3BO3["H3BO3"]
CultureMech_000864 --> ing7_H3BO3
CHEBI_33118["CHEBI:33118"]
ing7_H3BO3 -.-> CHEBI_33118
ing8_CoCl2_x_6_H2O["CoCl2 x 6 H2O"]
CultureMech_000864 --> ing8_CoCl2_x_6_H2O
CHEBI_53503["CHEBI:53503"]
ing8_CoCl2_x_6_H2O -.-> CHEBI_53503
ing9_CaCl2_x_2_H2O["CaCl2 x 2 H2O"]
CultureMech_000864 --> ing9_CaCl2_x_2_H2O
CHEBI_86158["CHEBI:86158"]
ing9_CaCl2_x_2_H2O -.-> CHEBI_86158
ing10_NiCl2_x_6_H2O["NiCl2 x 6 H2O"]
CultureMech_000864 --> ing10_NiCl2_x_6_H2O
CHEBI_34887["CHEBI:34887"]
ing10_NiCl2_x_6_H2O -.-> CHEBI_34887
ing11_Na2MoO4_x_2_H2O["Na2MoO4 x 2 H2O"]
CultureMech_000864 --> ing11_Na2MoO4_x_2_H2O
CHEBI_75213["CHEBI:75213"]
ing11_Na2MoO4_x_2_H2O -.-> CHEBI_75213
Ingredients (12)
| # | Ingredient | Concentration | Ontology | Notes |
|---|---|---|---|---|
| 1 |
(NH4)2SO4
2H4N.O4S 132.141 g/mol
|
0.05 G_PER_L |
CHEBI:62946
ammonium sulfate
|
|
| 2 |
MgCl2 x 6 H2O
2Cl.6H2O.Mg 203.301 g/mol
|
0.043000000000000003 G_PER_L |
CHEBI:86345
magnesium dichloride hexahydrate
|
[Merged 2 duplicates: 0.04, 0.003] |
| 3 |
KH2PO4
H2O4P.K 136.084 g/mol
|
0.07 G_PER_L |
CHEBI:63036
potassium dihydrogen phosphate
|
|
| 4 |
Methanol
CH4O 32.042 g/mol
|
0.5 G_PER_L |
CHEBI:17790
methanol
|
|
| 5 |
Na2-EDTA
C10H14N2O8.2Na 336.208 g/mol
|
0.5 G_PER_L |
CHEBI:64734
EDTA disodium salt (anhydrous)
|
|
| 6 |
FeSO4 x 7 H2O
Fe.7H2O.O4S 278.013 g/mol
|
0.2 G_PER_L |
CHEBI:75836
iron(2+) sulfate heptahydrate
|
|
| 7 |
ZnSO4 x 7 H2O
7H2O.O4S.Zn 287.558 g/mol
|
0.01 G_PER_L |
CHEBI:32312
zinc sulfate heptahydrate
|
|
| 8 |
H3BO3
H3BO3 61.833 g/mol
|
0.03 G_PER_L |
CHEBI:33118
boric acid
|
|
| 9 |
CoCl2 x 6 H2O
2Cl.Co.6H2O 237.929 g/mol
|
0.02 G_PER_L |
CHEBI:53503
cobalt chloride hexahydrate
|
|
| 10 |
CaCl2 x 2 H2O
Ca.2Cl.2H2O 147.014 g/mol
|
0.001 G_PER_L |
CHEBI:86158
calcium chloride dihydrate
|
|
| 11 |
NiCl2 x 6 H2O
Cl2Ni 129.599 g/mol
|
0.002 G_PER_L |
CHEBI:34887
nickel dichloride
|
|
| 12 |
Na2MoO4 x 2 H2O
2H2O.MoO4.2Na 241.946 g/mol
|
0.003 G_PER_L |
CHEBI:75213
sodium molybdate dihydrate
|
Preparation
- Prepare the liquid medium. Adjust to pH 6.0. Dispense the medium in tubes or flasks fitted with screw caps. Sterilize by autoclaving. Before inoculation add filter sterilized CH3OH to a final concentration of 0.5% v/v.
Target organisms
No target organisms are recorded.
Provenance
| Timestamp | Curator | Action | Notes |
|---|---|---|---|
| 2026-01-26T17:53:14.319976Z | mediadive-import | Imported from MediaDive | Source: DSMZ, ID: 1403 |
| 2026-03-13T03:08:39.219580Z | data-quality-cleanup-v1.0 | Data quality fixes | Merged 1 duplicate ingredient(s) |
| 2026-03-13T06:18:58.999102Z | culturemech-id-assigner-v1.0 | Assigned CultureMech ID | Assigned stable identifier: CultureMech:000864 |
| 2026-03-17T06:29:41.000000Z | mediaingredientmech-enrichment-v1.0 | Added MediaIngredientMech links | Linked ingredients to MediaIngredientMech identifiers |
| 2026-03-17T19:45:10.466947+00:00 | normalize_media_names_to_snake_case.py | NAME_NORMALIZED | Normalized name from "METHYLOROSULA MEDIUM V-022" to "methylorosula_medium_v_022" for consistent matching |
| 2026-06-05T07:41:33.377204+00:00 | mim-legacy-to-chebi-migration-v1.0 | Refreshed legacy MediaIngredientMech links to CHEBI keying | Replaced 12 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme. |
| 2026-06-10T07:47:07.653903+00:00 | primary-term-label-canonicalization-v1.0 | Canonicalized primary term label to OBO canonical (OAK) | 3 primary term(s); ingredient name resolved by OAK sqlite:obo:chebi. |