Overview
- External ontology
- mediadive.medium:1406 (source link unavailable) — HALARSENATIBACTER MEDIUM
- Applications
-
- Microbial cultivation
- pH
- 9.5
- High-metal flag
- True
- Notes
- Source: DSMZ | Link: https://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium1406.pdf
Composition
flowchart LR
CultureMech_000867["halarsenatibacter_medium"]
ing0_NaCl["NaCl"]
CultureMech_000867 --> ing0_NaCl
CHEBI_26710["CHEBI:26710"]
ing0_NaCl -.-> CHEBI_26710
ing1_Na2SO4["Na2SO4"]
CultureMech_000867 --> ing1_Na2SO4
CHEBI_32149["CHEBI:32149"]
ing1_Na2SO4 -.-> CHEBI_32149
ing2_K2SO4["K2SO4"]
CultureMech_000867 --> ing2_K2SO4
CHEBI_32036["CHEBI:32036"]
ing2_K2SO4 -.-> CHEBI_32036
ing3__NH4_2SO4["(NH4)2SO4"]
CultureMech_000867 --> ing3__NH4_2SO4
CHEBI_62946["CHEBI:62946"]
ing3__NH4_2SO4 -.-> CHEBI_62946
ing4_KH2PO4["KH2PO4"]
CultureMech_000867 --> ing4_KH2PO4
CHEBI_63036["CHEBI:63036"]
ing4_KH2PO4 -.-> CHEBI_63036
ing5_K2HPO4["K2HPO4"]
CultureMech_000867 --> ing5_K2HPO4
CHEBI_131527["CHEBI:131527"]
ing5_K2HPO4 -.-> CHEBI_131527
ing6_MgSO4_x_7_H2O["MgSO4 x 7 H2O"]
CultureMech_000867 --> ing6_MgSO4_x_7_H2O
CHEBI_31795["CHEBI:31795"]
ing6_MgSO4_x_7_H2O -.-> CHEBI_31795
ing7_Na2WO4["Na2WO4"]
CultureMech_000867 --> ing7_Na2WO4
CHEBI_63940["CHEBI:63940"]
ing7_Na2WO4 -.-> CHEBI_63940
ing8_H3BO3["H3BO3"]
CultureMech_000867 --> ing8_H3BO3
CHEBI_33118["CHEBI:33118"]
ing8_H3BO3 -.-> CHEBI_33118
ing9_Na2SeO4["Na2SeO4"]
CultureMech_000867 --> ing9_Na2SeO4
CHEBI_77775["CHEBI:77775"]
ing9_Na2SeO4 -.-> CHEBI_77775
ing10_Na2CO3["Na2CO3"]
CultureMech_000867 --> ing10_Na2CO3
CHEBI_29377["CHEBI:29377"]
ing10_Na2CO3 -.-> CHEBI_29377
ing11_NaHCO3["NaHCO3"]
CultureMech_000867 --> ing11_NaHCO3
CHEBI_32139["CHEBI:32139"]
ing11_NaHCO3 -.-> CHEBI_32139
ing12_Yeast_extract["Yeast extract"]
CultureMech_000867 --> ing12_Yeast_extract
ing13_Na_lactate["Na-lactate"]
CultureMech_000867 --> ing13_Na_lactate
CHEBI_75228["CHEBI:75228"]
ing13_Na_lactate -.-> CHEBI_75228
ing14_Arsenate["Arsenate"]
CultureMech_000867 --> ing14_Arsenate
CHEBI_29125["CHEBI:29125"]
ing14_Arsenate -.-> CHEBI_29125
ing15_Cysteine_HCl["Cysteine HCl"]
CultureMech_000867 --> ing15_Cysteine_HCl
ing16_Biotin["Biotin"]
CultureMech_000867 --> ing16_Biotin
CHEBI_15956["CHEBI:15956"]
ing16_Biotin -.-> CHEBI_15956
ing17_Folic_acid["Folic acid"]
CultureMech_000867 --> ing17_Folic_acid
CHEBI_27470["CHEBI:27470"]
ing17_Folic_acid -.-> CHEBI_27470
ing18_Pyridoxine_hydrochloride["Pyridoxine hydrochloride"]
CultureMech_000867 --> ing18_Pyridoxine_hydrochloride
CHEBI_30961["CHEBI:30961"]
ing18_Pyridoxine_hydrochloride -.-> CHEBI_30961
ing19_Thiamine_HCl_x_2_H2O["Thiamine-HCl x 2 H2O"]
CultureMech_000867 --> ing19_Thiamine_HCl_x_2_H2O
CHEBI_132751["CHEBI:132751"]
ing19_Thiamine_HCl_x_2_H2O -.-> CHEBI_132751
ing20_Riboflavin["Riboflavin"]
CultureMech_000867 --> ing20_Riboflavin
CHEBI_17015["CHEBI:17015"]
ing20_Riboflavin -.-> CHEBI_17015
ing21_Nicotinic_acid["Nicotinic acid"]
CultureMech_000867 --> ing21_Nicotinic_acid
CHEBI_15940["CHEBI:15940"]
ing21_Nicotinic_acid -.-> CHEBI_15940
ing22_D_Calcium_pantothenate["D-Calcium pantothenate"]
CultureMech_000867 --> ing22_D_Calcium_pantothenate
CHEBI_31345["CHEBI:31345"]
ing22_D_Calcium_pantothenate -.-> CHEBI_31345
ing23_Vitamin_B12["Vitamin B12"]
CultureMech_000867 --> ing23_Vitamin_B12
CHEBI_176843["CHEBI:176843"]
ing23_Vitamin_B12 -.-> CHEBI_176843
ing24_p_Aminobenzoic_acid["p-Aminobenzoic acid"]
CultureMech_000867 --> ing24_p_Aminobenzoic_acid
CHEBI_30753["CHEBI:30753"]
ing24_p_Aminobenzoic_acid -.-> CHEBI_30753
ing25_Lipoic_acid["Lipoic acid"]
CultureMech_000867 --> ing25_Lipoic_acid
CHEBI_16494["CHEBI:16494"]
ing25_Lipoic_acid -.-> CHEBI_16494
ing26_HCl["HCl"]
CultureMech_000867 --> ing26_HCl
CHEBI_17883["CHEBI:17883"]
ing26_HCl -.-> CHEBI_17883
ing27_FeSO4_x_7_H2O["FeSO4 x 7 H2O"]
CultureMech_000867 --> ing27_FeSO4_x_7_H2O
CHEBI_75836["CHEBI:75836"]
ing27_FeSO4_x_7_H2O -.-> CHEBI_75836
ing28_ZnCl2["ZnCl2"]
CultureMech_000867 --> ing28_ZnCl2
CHEBI_49976["CHEBI:49976"]
ing28_ZnCl2 -.-> CHEBI_49976
ing29_MnCl2_x_4_H2O["MnCl2 x 4 H2O"]
CultureMech_000867 --> ing29_MnCl2_x_4_H2O
CHEBI_86368["CHEBI:86368"]
ing29_MnCl2_x_4_H2O -.-> CHEBI_86368
CultureMech_000867 --> more["...4 more"]
Ingredients (34)
| # | Ingredient | Concentration | Ontology | Notes |
|---|---|---|---|---|
| 1 |
NaCl
Cl.Na 58.443 g/mol
|
151 G_PER_L |
CHEBI:26710
sodium chloride
|
|
| 2 |
Na2SO4
2Na.O4S 142.043 g/mol
|
84 G_PER_L |
CHEBI:32149
sodium sulfate
|
|
| 3 |
K2SO4
2K.O4S 174.259 g/mol
|
25 G_PER_L |
CHEBI:32036
potassium sulfate
|
|
| 4 |
(NH4)2SO4
2H4N.O4S 132.141 g/mol
|
0.05 G_PER_L |
CHEBI:62946
ammonium sulfate
|
|
| 5 |
KH2PO4
H2O4P.K 136.084 g/mol
|
0.08 G_PER_L |
CHEBI:63036
potassium dihydrogen phosphate
|
|
| 6 |
K2HPO4
HO4P.2K 174.174 g/mol
|
0.15 G_PER_L |
CHEBI:131527
dipotassium hydrogen phosphate
|
|
| 7 |
MgSO4 x 7 H2O
7H2O.Mg.O4S 246.473 g/mol
|
0.025 G_PER_L |
CHEBI:31795
magnesium sulfate heptahydrate
|
|
| 8 |
Na2WO4
2Na.O4W 293.816 g/mol
|
0.075 G_PER_L |
CHEBI:63940
sodium tungstate
|
|
| 9 |
H3BO3
H3BO3 61.833 g/mol
|
4.3 G_PER_L |
CHEBI:33118
boric acid
|
[Merged 2 duplicates: 4.0, 0.3] |
| 10 |
Na2SeO4
2Na.O4Se 188.936 g/mol
|
1e-05 G_PER_L |
CHEBI:77775
sodium selenate
|
|
| 11 |
Na2CO3
CO3.2Na 105.988 g/mol
|
36 G_PER_L |
CHEBI:29377
sodium carbonate
|
|
| 12 |
NaHCO3
CHO3.Na 84.006 g/mol
|
6 G_PER_L |
CHEBI:32139
sodium hydrogencarbonate
|
(after autoclaving) |
| 13 | Yeast extract | 1 G_PER_L | (after autoclaving) | |
| 14 |
Na-lactate
C3H5O3.Na 112.06 g/mol
|
1.1206 G_PER_L |
CHEBI:75228
sodium lactate
|
(after autoclaving) |
| 15 |
Arsenate
AsO4 138.918 g/mol
|
0.694595 G_PER_L |
CHEBI:29125
arsenate(3-)
|
(after autoclaving) |
| 16 | Cysteine HCl | 0.25 G_PER_L | (after autoclaving) | |
| 17 |
Biotin
C10H16N2O3S 244.316 g/mol
|
0.002 G_PER_L |
CHEBI:15956
biotin
|
|
| 18 |
Folic acid
C19H19N7O6 441.404 g/mol
|
0.002 G_PER_L |
CHEBI:27470
folic acid
|
|
| 19 |
Pyridoxine hydrochloride
C8H11NO3.HCl 205.641 g/mol
|
0.01 G_PER_L |
CHEBI:30961
pyridoxine hydrochloride
|
|
| 20 |
Thiamine-HCl x 2 H2O
C12H18N4OS.2Cl.2H2O 373.306 g/mol
|
0.005 G_PER_L |
CHEBI:132751
thiamine hydrochloride dihydrate
|
|
| 21 |
Riboflavin
C17H20N4O6 376.369 g/mol
|
0.005 G_PER_L |
CHEBI:17015
riboflavin
|
|
| 22 |
Nicotinic acid
C6H5NO2 123.111 g/mol
|
0.005 G_PER_L |
CHEBI:15940
nicotinic acid
|
|
| 23 |
D-Calcium pantothenate
2C9H16NO5.Ca 476.536 g/mol
|
0.005 G_PER_L |
CHEBI:31345
Calcium pantothenate
|
|
| 24 | Vitamin B12 | 0.0001 G_PER_L |
CHEBI:176843
vitamin B12
|
|
| 25 |
p-Aminobenzoic acid
C7H7NO2 137.138 g/mol
|
0.005 G_PER_L |
CHEBI:30753
4-aminobenzoic acid
|
|
| 26 |
Lipoic acid
C8H14O2S2 206.332 g/mol
|
0.005 G_PER_L |
CHEBI:16494
lipoic acid
|
|
| 27 |
HCl
HCl 36.461 g/mol
|
1.925 G_PER_L |
CHEBI:17883
hydrogen chloride
|
|
| 28 |
FeSO4 x 7 H2O
Fe.7H2O.O4S 278.013 g/mol
|
1.5 G_PER_L |
CHEBI:75836
iron(2+) sulfate heptahydrate
|
|
| 29 |
ZnCl2
2Cl.Zn 136.296 g/mol
|
0.07 G_PER_L |
CHEBI:49976
zinc dichloride
|
|
| 30 |
MnCl2 x 4 H2O
Cl2Mn.4H2O 197.904 g/mol
|
0.1 G_PER_L |
CHEBI:86368
manganese(II) chloride tetrahydrate
|
|
| 31 |
CoCl2 x 6 H2O
2Cl.Co.6H2O 237.929 g/mol
|
0.19 G_PER_L |
CHEBI:53503
cobalt chloride hexahydrate
|
|
| 32 |
CuCl2 x 2 H2O
Cl2Cu.2H2O 170.482 g/mol
|
0.002 G_PER_L |
CHEBI:86318
copper(II) chloride dihydrate
|
|
| 33 |
NiCl2 x 6 H2O
Cl2Ni 129.599 g/mol
|
0.024 G_PER_L |
CHEBI:34887
nickel dichloride
|
|
| 34 |
Na2MoO4 x 2 H2O
2H2O.MoO4.2Na 241.946 g/mol
|
0.036 G_PER_L |
CHEBI:75213
sodium molybdate dihydrate
|
Preparation
- Prepare the liquid medium without the following. NaHCO3, yeast extract, Na-lactate, arsenate. Adjust to pH 9.5. Boil the medium in a water bath and cool under N2. to room temperature. Dispense the medium into Hungate tubes or serum bottles under gassing with N2. Sterilize by autoclaving. Before inoculation add from sterile stock solutions:
Target organisms
No target organisms are recorded.
Provenance
| Timestamp | Curator | Action | Notes |
|---|---|---|---|
| 2026-01-26T17:53:14.328340Z | mediadive-import | Imported from MediaDive | Source: DSMZ, ID: 1406 |
| 2026-01-28T19:48:07.988014Z | chebi-enrichment | Added CHEBI ontology terms to ingredients | Enriched using MicrobeMediaParam and MediaDive chemical mappings |
| 2026-03-13T03:08:39.764037Z | data-quality-cleanup-v1.0 | Data quality fixes | Merged 1 duplicate ingredient(s) |
| 2026-03-13T06:18:59.028551Z | culturemech-id-assigner-v1.0 | Assigned CultureMech ID | Assigned stable identifier: CultureMech:000867 |
| 2026-03-17T06:29:41.000000Z | mediaingredientmech-enrichment-v1.0 | Added MediaIngredientMech links | Linked ingredients to MediaIngredientMech identifiers |
| 2026-03-17T19:45:10.500158+00:00 | normalize_media_names_to_snake_case.py | NAME_NORMALIZED | Normalized name from "HALARSENATIBACTER MEDIUM" to "halarsenatibacter_medium" for consistent matching |
| 2026-06-05T07:41:07.131569+00:00 | mim-legacy-to-chebi-migration-v1.0 | Refreshed legacy MediaIngredientMech links to CHEBI keying | Replaced 31 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme. |
| 2026-06-10T07:37:34.936860+00:00 | primary-term-regrounding-v1.0 | Re-grounded wrong primary term id (OAK-verified) | 1 primary term(s); ingredient name resolved by OAK sqlite:obo:chebi. |
| 2026-06-10T07:46:59.305657+00:00 | primary-term-label-canonicalization-v1.0 | Canonicalized primary term label to OBO canonical (OAK) | 10 primary term(s); ingredient name resolved by OAK sqlite:obo:chebi. |