Overview
- External ontology
- mediadive.medium:1410 (source link unavailable) — HALOBACTEROIDES MEDIUM
- Applications
-
- Microbial cultivation
- pH range
- {'min': 7.2, 'max': 7.4}
- High-metal flag
- True
- Notes
- Source: DSMZ | Link: https://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium1410.pdf
Composition
flowchart LR
CultureMech_000872["halobacteroides_medium"]
ing0_NH4Cl["NH4Cl"]
CultureMech_000872 --> ing0_NH4Cl
CHEBI_31206["CHEBI:31206"]
ing0_NH4Cl -.-> CHEBI_31206
ing1_K2HPO4["K2HPO4"]
CultureMech_000872 --> ing1_K2HPO4
CHEBI_131527["CHEBI:131527"]
ing1_K2HPO4 -.-> CHEBI_131527
ing2_KH2PO4["KH2PO4"]
CultureMech_000872 --> ing2_KH2PO4
CHEBI_63036["CHEBI:63036"]
ing2_KH2PO4 -.-> CHEBI_63036
ing3_CaCl2_x_2_H2O["CaCl2 x 2 H2O"]
CultureMech_000872 --> ing3_CaCl2_x_2_H2O
CHEBI_86158["CHEBI:86158"]
ing3_CaCl2_x_2_H2O -.-> CHEBI_86158
ing4_NaCl["NaCl"]
CultureMech_000872 --> ing4_NaCl
CHEBI_26710["CHEBI:26710"]
ing4_NaCl -.-> CHEBI_26710
ing5_KCl["KCl"]
CultureMech_000872 --> ing5_KCl
CHEBI_32588["CHEBI:32588"]
ing5_KCl -.-> CHEBI_32588
ing6_KNO3["KNO3"]
CultureMech_000872 --> ing6_KNO3
CHEBI_63043["CHEBI:63043"]
ing6_KNO3 -.-> CHEBI_63043
ing7_Glucose["Glucose"]
CultureMech_000872 --> ing7_Glucose
CHEBI_17234["CHEBI:17234"]
ing7_Glucose -.-> CHEBI_17234
ing8_Yeast_extract["Yeast extract"]
CultureMech_000872 --> ing8_Yeast_extract
ing9_MgCl2_x_6_H2O["MgCl2 x 6 H2O"]
CultureMech_000872 --> ing9_MgCl2_x_6_H2O
CHEBI_86345["CHEBI:86345"]
ing9_MgCl2_x_6_H2O -.-> CHEBI_86345
ing10_NaHCO3["NaHCO3"]
CultureMech_000872 --> ing10_NaHCO3
CHEBI_32139["CHEBI:32139"]
ing10_NaHCO3 -.-> CHEBI_32139
ing11_Nitrilotriacetic_acid["Nitrilotriacetic acid"]
CultureMech_000872 --> ing11_Nitrilotriacetic_acid
CHEBI_44557["CHEBI:44557"]
ing11_Nitrilotriacetic_acid -.-> CHEBI_44557
ing12_MgSO4_x_7_H2O["MgSO4 x 7 H2O"]
CultureMech_000872 --> ing12_MgSO4_x_7_H2O
CHEBI_31795["CHEBI:31795"]
ing12_MgSO4_x_7_H2O -.-> CHEBI_31795
ing13_MnSO4_x_H2O["MnSO4 x H2O"]
CultureMech_000872 --> ing13_MnSO4_x_H2O
CHEBI_86364["CHEBI:86364"]
ing13_MnSO4_x_H2O -.-> CHEBI_86364
ing14_FeSO4_x_7_H2O["FeSO4 x 7 H2O"]
CultureMech_000872 --> ing14_FeSO4_x_7_H2O
CHEBI_75836["CHEBI:75836"]
ing14_FeSO4_x_7_H2O -.-> CHEBI_75836
ing15_CoSO4_x_7_H2O["CoSO4 x 7 H2O"]
CultureMech_000872 --> ing15_CoSO4_x_7_H2O
CHEBI_91244["CHEBI:91244"]
ing15_CoSO4_x_7_H2O -.-> CHEBI_91244
ing16_ZnSO4_x_7_H2O["ZnSO4 x 7 H2O"]
CultureMech_000872 --> ing16_ZnSO4_x_7_H2O
CHEBI_32312["CHEBI:32312"]
ing16_ZnSO4_x_7_H2O -.-> CHEBI_32312
ing17_CuSO4_x_5_H2O["CuSO4 x 5 H2O"]
CultureMech_000872 --> ing17_CuSO4_x_5_H2O
CHEBI_31440["CHEBI:31440"]
ing17_CuSO4_x_5_H2O -.-> CHEBI_31440
ing18_AlK_SO4_2_x_12_H2O["AlK(SO4)2 x 12 H2O"]
CultureMech_000872 --> ing18_AlK_SO4_2_x_12_H2O
CHEBI_86465["CHEBI:86465"]
ing18_AlK_SO4_2_x_12_H2O -.-> CHEBI_86465
ing19_H3BO3["H3BO3"]
CultureMech_000872 --> ing19_H3BO3
CHEBI_33118["CHEBI:33118"]
ing19_H3BO3 -.-> CHEBI_33118
ing20_Na2MoO4_x_2_H2O["Na2MoO4 x 2 H2O"]
CultureMech_000872 --> ing20_Na2MoO4_x_2_H2O
CHEBI_75213["CHEBI:75213"]
ing20_Na2MoO4_x_2_H2O -.-> CHEBI_75213
ing21_NiCl2_x_6_H2O["NiCl2 x 6 H2O"]
CultureMech_000872 --> ing21_NiCl2_x_6_H2O
CHEBI_34887["CHEBI:34887"]
ing21_NiCl2_x_6_H2O -.-> CHEBI_34887
ing22_Na2SeO3_x_5_H2O["Na2SeO3 x 5 H2O"]
CultureMech_000872 --> ing22_Na2SeO3_x_5_H2O
CHEBI_131361["CHEBI:131361"]
ing22_Na2SeO3_x_5_H2O -.-> CHEBI_131361
Ingredients (23)
| # | Ingredient | Concentration | Ontology | Notes |
|---|---|---|---|---|
| 1 |
NH4Cl
Cl.H4N 53.492 g/mol
|
1 G_PER_L |
CHEBI:31206
ammonium chloride
|
|
| 2 |
K2HPO4
HO4P.2K 174.174 g/mol
|
0.3 G_PER_L |
CHEBI:131527
dipotassium hydrogen phosphate
|
|
| 3 |
KH2PO4
H2O4P.K 136.084 g/mol
|
0.3 G_PER_L |
CHEBI:63036
potassium dihydrogen phosphate
|
|
| 4 |
CaCl2 x 2 H2O
Ca.2Cl.2H2O 147.014 g/mol
|
0.1 G_PER_L |
CHEBI:86158
calcium chloride dihydrate
|
[Collapsed 2 identical duplicates: 0.1, 0.1] |
| 5 |
NaCl
Cl.Na 58.443 g/mol
|
251.0 G_PER_L |
CHEBI:26710
sodium chloride
|
[Merged 2 duplicates: 250.0, 1.0] |
| 6 |
KCl
Cl.K 74.551 g/mol
|
0.1 G_PER_L |
CHEBI:32588
potassium chloride
|
|
| 7 |
KNO3
K.NO3 101.102 g/mol
|
2 G_PER_L |
CHEBI:63043
potassium nitrate
|
|
| 8 |
Glucose
C6H12O6 180.156 g/mol
|
4 G_PER_L |
CHEBI:17234
glucose
|
|
| 9 | Yeast extract | 1 G_PER_L | ||
| 10 |
MgCl2 x 6 H2O
2Cl.6H2O.Mg 203.301 g/mol
|
1 G_PER_L |
CHEBI:86345
magnesium dichloride hexahydrate
|
|
| 11 |
NaHCO3
CHO3.Na 84.006 g/mol
|
2 G_PER_L |
CHEBI:32139
sodium hydrogencarbonate
|
|
| 12 |
Nitrilotriacetic acid
C6H9NO6 191.139 g/mol
|
1.5 G_PER_L |
CHEBI:44557
nitrilotriacetic acid
|
|
| 13 |
MgSO4 x 7 H2O
7H2O.Mg.O4S 246.473 g/mol
|
3 G_PER_L |
CHEBI:31795
magnesium sulfate heptahydrate
|
|
| 14 |
MnSO4 x H2O
H2O.Mn.O4S 169.016 g/mol
|
0.5 G_PER_L |
CHEBI:86364
manganese(II) sulfate monohydrate
|
|
| 15 |
FeSO4 x 7 H2O
Fe.7H2O.O4S 278.013 g/mol
|
0.1 G_PER_L |
CHEBI:75836
iron(2+) sulfate heptahydrate
|
|
| 16 |
CoSO4 x 7 H2O
Co.7H2O.O4S 281.101 g/mol
|
0.18 G_PER_L |
CHEBI:91244
cobalt(2+) sulfate heptahydrate
|
|
| 17 |
ZnSO4 x 7 H2O
7H2O.O4S.Zn 287.558 g/mol
|
0.18 G_PER_L |
CHEBI:32312
zinc sulfate heptahydrate
|
|
| 18 |
CuSO4 x 5 H2O
Cu.5H2O.O4S 249.684 g/mol
|
0.01 G_PER_L |
CHEBI:31440
copper(II) sulfate pentahydrate
|
|
| 19 |
AlK(SO4)2 x 12 H2O
Al.12H2O.K.2O4S 474.386 g/mol
|
0.02 G_PER_L |
CHEBI:86465
potassium aluminium sulfate dodecahydrate
|
|
| 20 |
H3BO3
H3BO3 61.833 g/mol
|
0.01 G_PER_L |
CHEBI:33118
boric acid
|
|
| 21 |
Na2MoO4 x 2 H2O
2H2O.MoO4.2Na 241.946 g/mol
|
0.01 G_PER_L |
CHEBI:75213
sodium molybdate dihydrate
|
|
| 22 |
NiCl2 x 6 H2O
Cl2Ni 129.599 g/mol
|
0.03 G_PER_L |
CHEBI:34887
nickel dichloride
|
|
| 23 |
Na2SeO3 x 5 H2O
5H2O.2Na.O3Se 263.012 g/mol
|
0.0003 G_PER_L |
CHEBI:131361
disodium selenite pentahydrate
|
Preparation
- Prepare the liquid medium. Adjust to pH 6.3 with 10 M KOH solution. Boil the medium in a water bath and cool under N2:CO2 (80:20 v/v) to room temperature. Dispense the medium into Hungate tubes or serum bottles under gassing with N2:CO2 (80:20 v/v). Sterilize by autoclaving. Before inoculation add from sterile stock solutions:
- Final pH 7.2 - 7.4
Target organisms
No target organisms are recorded.
Provenance
| Timestamp | Curator | Action | Notes |
|---|---|---|---|
| 2026-01-26T17:53:14.339510Z | mediadive-import | Imported from MediaDive | Source: DSMZ, ID: 1410 |
| 2026-03-13T03:09:01.129258Z | data-quality-cleanup-v1.0 | Data quality fixes | Merged 2 duplicate ingredient(s) |
| 2026-03-13T06:18:59.066105Z | culturemech-id-assigner-v1.0 | Assigned CultureMech ID | Assigned stable identifier: CultureMech:000872 |
| 2026-03-17T06:30:06.000000Z | mediaingredientmech-enrichment-v1.0 | Added MediaIngredientMech links | Linked ingredients to MediaIngredientMech identifiers |
| 2026-03-17T19:45:10.542439+00:00 | normalize_media_names_to_snake_case.py | NAME_NORMALIZED | Normalized name from "HALOBACTEROIDES MEDIUM" to "halobacteroides_medium" for consistent matching |
| 2026-05-20T06:01:21.971661+00:00 | migrate_ph_range.py | MIGRATED_PH_RANGE | ph_range '7.2-7.4' -> {'min': 7.2, 'max': 7.4} |
| 2026-06-05T07:41:07.663263+00:00 | mim-legacy-to-chebi-migration-v1.0 | Refreshed legacy MediaIngredientMech links to CHEBI keying | Replaced 21 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme. |
| 2026-06-10T07:37:34.943591+00:00 | primary-term-regrounding-v1.0 | Re-grounded wrong primary term id (OAK-verified) | 1 primary term(s); ingredient name resolved by OAK sqlite:obo:chebi. |
| 2026-06-10T07:46:59.478446+00:00 | primary-term-label-canonicalization-v1.0 | Canonicalized primary term label to OBO canonical (OAK) | 5 primary term(s); ingredient name resolved by OAK sqlite:obo:chebi. |
| 2026-09-02T07:38:10.121245+00:00 | repair_merged_duplicates.py | REPAIRED_SUMMED_DUPLICATE_MERGE | Collapsed 1 ingredient row(s) whose concentration was the SUM of identical duplicate values written by cleanup_media_quality.py. Every merged part was the same value, so the pre-merge value is unambiguous and no source lookup was needed. Rows whose merged parts differ were left untouched. |