amphibacillus_medium

CultureMech:000875 bacterial COMPLEX LIQUID
Original name: AMPHIBACILLUS MEDIUM

Overview

External ontology
mediadive.medium:1413 (source link unavailable) — AMPHIBACILLUS MEDIUM
Applications
  • Microbial cultivation
pH
10.0
Notes
Source: DSMZ | Link: https://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium1413.pdf

Composition

flowchart LR
    CultureMech_000875["amphibacillus_medium"]
    ing0_NaHCO3["NaHCO3"]
    CultureMech_000875 --> ing0_NaHCO3
    CHEBI_32139["CHEBI:32139"]
    ing0_NaHCO3 -.-> CHEBI_32139
    ing1_Na2CO3["Na2CO3"]
    CultureMech_000875 --> ing1_Na2CO3
    CHEBI_29377["CHEBI:29377"]
    ing1_Na2CO3 -.-> CHEBI_29377
    ing2_NaCl["NaCl"]
    CultureMech_000875 --> ing2_NaCl
    CHEBI_26710["CHEBI:26710"]
    ing2_NaCl -.-> CHEBI_26710
    ing3_K2HPO4["K2HPO4"]
    CultureMech_000875 --> ing3_K2HPO4
    CHEBI_131527["CHEBI:131527"]
    ing3_K2HPO4 -.-> CHEBI_131527
    ing4_MgSO4["MgSO4"]
    CultureMech_000875 --> ing4_MgSO4
    CHEBI_32599["CHEBI:32599"]
    ing4_MgSO4 -.-> CHEBI_32599
    ing5_Yeast_extract["Yeast extract"]
    CultureMech_000875 --> ing5_Yeast_extract
    ing6_Glucose["Glucose"]
    CultureMech_000875 --> ing6_Glucose
    CHEBI_17234["CHEBI:17234"]
    ing6_Glucose -.-> CHEBI_17234
    ing7_EDTA["EDTA"]
    CultureMech_000875 --> ing7_EDTA
    CHEBI_4735["CHEBI:4735"]
    ing7_EDTA -.-> CHEBI_4735
    ing8_FeSO4_x_7_H2O["FeSO4 x 7 H2O"]
    CultureMech_000875 --> ing8_FeSO4_x_7_H2O
    CHEBI_75836["CHEBI:75836"]
    ing8_FeSO4_x_7_H2O -.-> CHEBI_75836
    ing9_ZnSO4_x_7_H2O["ZnSO4 x 7 H2O"]
    CultureMech_000875 --> ing9_ZnSO4_x_7_H2O
    CHEBI_32312["CHEBI:32312"]
    ing9_ZnSO4_x_7_H2O -.-> CHEBI_32312
    ing10_MnCl2_x_4_H2O["MnCl2 x 4 H2O"]
    CultureMech_000875 --> ing10_MnCl2_x_4_H2O
    CHEBI_86368["CHEBI:86368"]
    ing10_MnCl2_x_4_H2O -.-> CHEBI_86368
    ing11_H3BO3["H3BO3"]
    CultureMech_000875 --> ing11_H3BO3
    CHEBI_33118["CHEBI:33118"]
    ing11_H3BO3 -.-> CHEBI_33118
    ing12_CoCl2_x_6_H2O["CoCl2 x 6 H2O"]
    CultureMech_000875 --> ing12_CoCl2_x_6_H2O
    CHEBI_53503["CHEBI:53503"]
    ing12_CoCl2_x_6_H2O -.-> CHEBI_53503
    ing13_CuCl2_x_2_H2O["CuCl2 x 2 H2O"]
    CultureMech_000875 --> ing13_CuCl2_x_2_H2O
    CHEBI_86318["CHEBI:86318"]
    ing13_CuCl2_x_2_H2O -.-> CHEBI_86318
    ing14_NiCl2_x_6_H2O["NiCl2 x 6 H2O"]
    CultureMech_000875 --> ing14_NiCl2_x_6_H2O
    CHEBI_34887["CHEBI:34887"]
    ing14_NiCl2_x_6_H2O -.-> CHEBI_34887
    ing15_Na2MoO4_x_2_H2O["Na2MoO4 x 2 H2O"]
    CultureMech_000875 --> ing15_Na2MoO4_x_2_H2O
    CHEBI_75213["CHEBI:75213"]
    ing15_Na2MoO4_x_2_H2O -.-> CHEBI_75213

Ingredients (16)

# Ingredient Concentration Ontology Notes
1 NaHCO3
CHO3.Na 84.006 g/mol
15 G_PER_L CHEBI:32139
sodium hydrogencarbonate
2 Na2CO3
CO3.2Na 105.988 g/mol
95 G_PER_L CHEBI:29377
sodium carbonate
3 NaCl
Cl.Na 58.443 g/mol
16 G_PER_L CHEBI:26710
sodium chloride
4 K2HPO4
HO4P.2K 174.174 g/mol
1 G_PER_L CHEBI:131527
dipotassium hydrogen phosphate
5 MgSO4
Mg.O4S 120.368 g/mol
0.12037 G_PER_L CHEBI:32599
magnesium sulfate
6 Yeast extract 0.5 G_PER_L
7 Glucose
C6H12O6 180.156 g/mol
2 G_PER_L CHEBI:17234
glucose
8 EDTA
C10H16N2O8 292.244 g/mol
5 G_PER_L CHEBI:4735
ethylenediaminetetraacetic acid
9 FeSO4 x 7 H2O
Fe.7H2O.O4S 278.013 g/mol
2.2 G_PER_L CHEBI:75836
iron(2+) sulfate heptahydrate
10 ZnSO4 x 7 H2O
7H2O.O4S.Zn 287.558 g/mol
0.1 G_PER_L CHEBI:32312
zinc sulfate heptahydrate
11 MnCl2 x 4 H2O
Cl2Mn.4H2O 197.904 g/mol
0.03 G_PER_L CHEBI:86368
manganese(II) chloride tetrahydrate
12 H3BO3
H3BO3 61.833 g/mol
0.03 G_PER_L CHEBI:33118
boric acid
13 CoCl2 x 6 H2O
2Cl.Co.6H2O 237.929 g/mol
0.2 G_PER_L CHEBI:53503
cobalt chloride hexahydrate
14 CuCl2 x 2 H2O
Cl2Cu.2H2O 170.482 g/mol
0.03 G_PER_L CHEBI:86318
copper(II) chloride dihydrate
15 NiCl2 x 6 H2O
Cl2Ni 129.599 g/mol
0.03 G_PER_L CHEBI:34887
nickel dichloride
16 Na2MoO4 x 2 H2O
2H2O.MoO4.2Na 241.946 g/mol
0.03 G_PER_L CHEBI:75213
sodium molybdate dihydrate

Preparation

  1. Prepare the liquid medium. Dispense the medium into serum bottles. After sterilization pH 10.0. Before inoculation add from sterile stock solutions:
  2. pH 3.0-4.0

Target organisms

No target organisms are recorded.

Provenance

TimestampCuratorActionNotes
2026-01-26T17:53:14.351133Z mediadive-import Imported from MediaDive Source: DSMZ, ID: 1413
2026-03-13T06:18:59.096919Z culturemech-id-assigner-v1.0 Assigned CultureMech ID Assigned stable identifier: CultureMech:000875
2026-03-17T06:29:58.000000Z mediaingredientmech-enrichment-v1.0 Added MediaIngredientMech links Linked ingredients to MediaIngredientMech identifiers
2026-03-17T19:45:10.575685+00:00 normalize_media_names_to_snake_case.py NAME_NORMALIZED Normalized name from "AMPHIBACILLUS MEDIUM" to "amphibacillus_medium" for consistent matching
2026-06-05T07:40:48.632606+00:00 mim-legacy-to-chebi-migration-v1.0 Refreshed legacy MediaIngredientMech links to CHEBI keying Replaced 15 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme.
2026-06-10T07:46:53.081209+00:00 primary-term-label-canonicalization-v1.0 Canonicalized primary term label to OBO canonical (OAK) 4 primary term(s); ingredient name resolved by OAK sqlite:obo:chebi.